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Romay C, Gonzalez R, Pizarro M, Lissi E
J Protein Chem 19 (2) 151-155 [2000-02-00; online 2000-08-17]
Hydrochlorous acid bleaches c-phycocyanin visible absorbance with a second-order rate constant (pH 7.4) of 1.3x10(3) M(-1) s(-1). In excess of protein, ca. 0.16 bilin moieties are disrupted by each reacted HOCl molecule. This indicates that the main reaction takes place at the apoprotein level, with a total rate constant (in monomeric units concentration) of 2.5x10(4) M(-1) s(-1). This rate constant is too low to provide protection to other biomolecules under physiological conditions. The reported antiinflammatory properties of phycocyanin are not then related to the removal of HOCl. On the other hand, the rather slow reaction rate with HOCI could be beneficial to its role as antiinflammatory agent since it will allow the protein to maintain its integrity at the inflammation locus.
Glycoproteomics and MS Proteomics [Service]
PubMed 10945439
DOI 10.1023/a:1007038801482
Crossref 10.1023/a:1007038801482