(R,S)-dichlorprop herbicide in agricultural soil induces proliferation and expression of multiple dioxygenase-encoding genes in the indigenous microbial community.

Paulin MM, Nicolaisen MH, Sørensen J

Environ. Microbiol. 13 (6) 1513-1523 [2011-06-00; online 2011-03-23]

We investigated the effect of (R,S)-dichlorprop herbicide addition to soil microcosms on the degrading indigenous microbial community by targeting multiple α-ketoglutarate-dependent (α-KG) dioxygenase-encoding genes (rdpA, sdpA and tfdA group I) at both gene and transcript level. The soil microbial community responded with high growth of potential degraders as measured by the abundance of dioxygenase-encoding genes using quantitative real-time PCR (qPCR). rdpA DNA was not detectable in unamended soil but reached over 10⁶ copies g⁻¹ soil after amendment. sdpA and tfdA were both present prior to amendment at levels of ~5 × 10⁴ and ~ 10² copies g⁻¹ soil, respectively, and both reached over 10⁵copies g⁻¹ soil. While expression of all three target genes was detected during two cycles of herbicide degradation, a time-shift occurred between maximum expression of each gene. Gene diversity by denaturing gradient gel electrophoresis (DGGE) uncovered a diversity of sdpA and tfdA genes at the DNA level while rdpA remained highly conserved. However, mRNA profiles indicated that all transcribed tfdA sequences were class III genes while rdpA transcripts shared 100% identity to rdpA of Delftia acidovorans MC1 and sdpA transcripts shared 100% identity to sdpA from Sphingomonas herbicidovorans MH. This is the first report to describe expression dynamics of multiple α-KG dioxygenase-encoding genes in the indigenous microbial community as related to degradation of a phenoxypropionate herbicide in soil.

NGI Uppsala (Uppsala Genome Center)

National Genomics Infrastructure

PubMed 21418495

DOI 10.1111/j.1462-2920.2011.02456.x

Crossref 10.1111/j.1462-2920.2011.02456.x

GENBANK: HQ009355
GENBANK: HQ009356
GENBANK: HQ009357
GENBANK: HQ009358
GENBANK: HQ009359
GENBANK: HQ009360
GENBANK: HQ009361
GENBANK: HQ009362
GENBANK: HQ009363
GENBANK: HQ009364
GENBANK: HQ009365
GENBANK: HQ009366
GENBANK: HQ009367
GENBANK: HQ009368
GENBANK: HQ009369
GENBANK: HQ009370
GENBANK: HQ009371
GENBANK: HQ009372
GENBANK: HQ009373
GENBANK: HQ009374
GENBANK: HQ009375
GENBANK: HQ009376
GENBANK: HQ009377
GENBANK: HQ009378
GENBANK: HQ009379
GENBANK: HQ009380
GENBANK: HQ009381
GENBANK: HQ009382
GENBANK: HQ009383
GENBANK: HQ009384
GENBANK: HQ009385
GENBANK: HQ009386
GENBANK: HQ009387
GENBANK: HQ009388
GENBANK: HQ009389
GENBANK: HQ009390
GENBANK: HQ009391
GENBANK: HQ009392
GENBANK: HQ009393
GENBANK: HQ009394
GENBANK: HQ009395
GENBANK: HQ009396
GENBANK: HQ009397
GENBANK: HQ009398