Identification and verification of microRNAs by high-throughput sequencing.

Hällman J, Avesson L, Reimegård J, Käller M, Söderbom F

Methods Mol. Biol. 983 (-) 125-138 [2013-03-16; online 2013-03-16]

High-throughput sequencing methods have become invaluable for detection and analysis of small RNAs. The results are millions of sequences that need to be carefully analyzed by computational methods and preferentially verified by different experimental techniques. Here we describe how to use high-throughput sequencing followed by bioinformatics and northern blot to identify one particular class of small RNA, microRNAs.

NGI Stockholm (Genomics Applications)

NGI Stockholm (Genomics Production)

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PubMed 23494305

DOI 10.1007/978-1-62703-302-2_7

Crossref 10.1007/978-1-62703-302-2_7