Is label-free LC-MS/MS ready for biomarker discovery?

Sandin M, Chawade A, Levander F

Proteomics Clin Appl 9 (3-4) 289-294 [2015-04-00; online 2015-02-07]

Label-free LC-MS methods are attractive for high-throughput quantitative proteomics, as the sample processing is straightforward and can be scaled to a large number of samples. Label-free methods therefore facilitate biomarker discovery in studies involving dozens of clinical samples. However, despite the increased popularity of label-free workflows, there is a hesitance in the research community to use it in clinical proteomics studies. Therefore, we here discuss pros and cons of label-free LC-MS/MS for biomarker discovery, and delineate the main prerequisites for its successful employment. Furthermore, we cite studies where label-free LC-MS/MS was successfully used to identify novel biomarkers, and foresee an increased acceptance of label-free techniques by the proteomics community in the near future.

Bioinformatics Support and Infrastructure

QC bibliography QC xrefs

PubMed 25656266

DOI 10.1002/prca.201400202

Crossref 10.1002/prca.201400202