CUBIC-f: An optimized clearing method for cell tracing and evaluation of neurite density in the salamander brain

Pinheiro T, Mayor I, Edwards S, Joven A, Kantzer CG, Kirkham M, Simon A

Journal of Neuroscience Methods 348 (-) 109002 [2021-01-00; online 2021-01-00]

Although tissue clearing and subsequent whole-brain imaging is now possible, standard protocols need to be adjusted to the innate properties of each specific tissue for optimal results. This work modifies exiting protocols to clear fragile brain samples and documents a downstream pipeline for image processing and data analysis.

Integrated Microscopy Technologies Stockholm [Technology development]

QC bibliography QC xrefs

DOI 10.1016/j.jneumeth.2020.109002

Crossref 10.1016/j.jneumeth.2020.109002