Collecting and preserving biological samples from challenging environments for DNA analysis.

Buś MM, Allen M

Biopreserv Biobank 12 (1) 17-22 [2014-02-00; online 2014-03-14]

Biological materials collected in harsh environments such as archaeological excavations, at crime scenes, after mass disasters, in museums, or non-invasively in the field constitute a highly valuable source of genetic information. However, poor quality and limited quantity of the DNA extracted from these samples can be extremely challenging during further analyses. Here we have reviewed how degradation, decomposition, and contamination can affect DNA analysis, and how correct sample collection and storage methods will ensure the best possible conditions for further genetic analysis. Furthermore, highly efficient protocols for collection, decontamination, and extraction of DNA from minute amounts of biological material are presented.

NGI Stockholm (Genomics Applications)

NGI Stockholm (Genomics Production)

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PubMed 24620766

DOI 10.1089/bio.2013.0060

Crossref 10.1089/bio.2013.0060