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Malmström E, Malmström L, Hauri S, Mohanty T, Scott A, Karlsson C, Gueto-Tettay C, Åhrman E, Nozohoor S, Tingstedt B, Regner S, Elfving P, Bjermer L, Forsvall A, Doyle A, Magnusson M, Hedenfalk I, Kannisto P, Brandt C, Nilsson E, Dahlin LB, Malm J, Linder A, Niméus E, Malmström J
Cell 188 (10) 2810-2822.e16 [2025-05-15; online 2025-04-08]
The plasma proteome is maintained by the influx and efflux of proteins from surrounding organs and cells. To quantify the extent to which different organs and cells impact the plasma proteome in healthy and diseased conditions, we developed a mass-spectrometry-based proteomics strategy to infer the tissue origin of proteins detected in human plasma. We first constructed an extensive human proteome atlas from 18 vascularized organs and the 8 most abundant cell types in blood. The atlas was interfaced with previous RNA and protein atlases to objectively define proteome-wide protein-organ associations to infer the origin and enable the reproducible quantification of organ-specific proteins in plasma. We demonstrate that the resource can determine disease-specific quantitative changes of organ-enriched protein panels in six separate patient cohorts, including sepsis, pancreatitis, and myocardial injury. The strategy can be extended to other diseases to advance our understanding of the processes contributing to plasma proteome dynamics.
Clinical Proteomics Lund [Technology development]
PubMed 40203824
DOI 10.1016/j.cell.2025.03.013
Crossref 10.1016/j.cell.2025.03.013
pii: S0092-8674(25)00286-7