Mapping-by-sequencing accelerates forward genetics in barley.

Mascher M, Jost M, Kuon JE, Himmelbach A, AƟfalg A, Beier S, Scholz U, Graner A, Stein N

Genome Biol. 15 (6) R78 [2014-06-10; online 2014-06-10]

Mapping-by-sequencing has emerged as a powerful technique for genetic mapping in several plant and animal species. As this resequencing-based method requires a reference genome, its application to complex plant genomes with incomplete and fragmented sequence resources remains challenging. We perform exome sequencing of phenotypic bulks of a mapping population of barley segregating for a mutant phenotype that increases the rate of leaf initiation. Read depth analysis identifies a candidate gene, which is confirmed by the analysis of independent mutant alleles. Our method illustrates how the genomic resources of barley together with exome resequencing can underpin mapping-by-sequencing.

NGI Stockholm (Genomics Applications)

NGI Stockholm (Genomics Production)

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PubMed 24917130

DOI 10.1186/gb-2014-15-6-r78

Crossref 10.1186/gb-2014-15-6-r78

gb-2014-15-6-r78

pmc PMC4073093