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Pustovalova Y, Mayzel M, Orekhov VY
Angew. Chem. Int. Ed. 57 (43) 14043-14045 [2018-10-22; online 2018-10-01]
NMR studies of intrinsically disordered proteins and other complex biomolecular systems require spectra with the highest resolution and dimensionality. An efficient approach, extra-large NMR spectroscopy, is presented for experimental data collection, reconstruction, and handling of very large NMR spectra by a combination of the radial and non-uniform sampling, a new processing algorithm, and rigorous statistical validation. We demonstrate the first high-quality reconstruction of a full seven-dimensional HNCOCACONH and two five-dimensional HACACONH and HN(CA)CONH experiments for a representative intrinsically disordered protein α-synuclein. XLSY will significantly enhance the NMR toolbox in challenging biomolecular studies.
Swedish NMR Centre (SNC) [Technology development]
PubMed 30175546
DOI 10.1002/anie.201806144
Crossref 10.1002/anie.201806144