The age and genomic integrity of neurons after cortical stroke in humans.

Huttner HB, Bergmann O, Salehpour M, Rácz A, Tatarishvili J, Lindgren E, Csonka T, Csiba L, Hortobágyi T, Méhes G, Englund E, Solnestam BW, Zdunek S, Scharenberg C, Ström L, Ståhl P, Sigurgeirsson B, Dahl A, Schwab S, Possnert G, Bernard S, Kokaia Z, Lindvall O, Lundeberg J, Frisén J

Nat. Neurosci. 17 (6) 801-803 [2014-06-00; online 2014-04-22]

It has been unclear whether ischemic stroke induces neurogenesis or neuronal DNA rearrangements in the human neocortex. Using immunohistochemistry; transcriptome, genome and ploidy analyses; and determination of nuclear bomb test-derived (14)C concentration in neuronal DNA, we found neither to be the case. A large proportion of cortical neurons displayed DNA fragmentation and DNA repair a short time after stroke, whereas neurons at chronic stages after stroke showed DNA integrity, demonstrating the relevance of an intact genome for survival.

NGI Stockholm (Genomics Applications)

NGI Stockholm (Genomics Production)

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PubMed 24747576

DOI 10.1038/nn.3706

Crossref 10.1038/nn.3706

GEO GSE56267

SRA SRP040676

nn.3706