Effect of RNA silencing suppression activity of chrysanthemum virus B p12 protein on small RNA species.

Vetukuri RR, Kalyandurg PB, Saripella GV, Sen D, Gil JF, Lukhovitskaya NI, Grenville-Briggs LJ, Savenkov EI

Arch Virol 165 (12) 2953-2959 [2020-12-00; online 2020-10-10]

Chrysanthemum virus B encodes a multifunctional p12 protein that acts as a transcriptional activator in the nucleus and as a suppressor of RNA silencing in the cytoplasm. Here, we investigated the impact of p12 on accumulation of major classes of small RNAs (sRNAs). The results show dramatic changes in the sRNA profiles characterised by an overall reduction in sRNA accumulation, changes in the pattern of size distribution of canonical siRNAs and in the ratio between sense and antisense strands, lower abundance of siRNAs with a U residue at the 5'-terminus, and changes in the expression of certain miRNAs, most of which were downregulated.

NGI Stockholm (Genomics Applications) [Service]

NGI Stockholm (Genomics Production) [Service]

National Genomics Infrastructure [Service]

PubMed 33040310

DOI 10.1007/s00705-020-04832-y

Crossref 10.1007/s00705-020-04832-y

pii: 10.1007/s00705-020-04832-y
pmc: PMC7588395