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Liebmann T, Blom H, Aperia A, Brismar H
Optical Nanoscopy Opt Nanoscopy 2 (1) 6 [2013-00-00; online 2013-00-00]
Integrated Microscopy Technologies Stockholm [Technology development]
DOI 10.1186/2192-2853-2-6
Crossref 10.1186/2192-2853-2-6
We have utilized PALM for dissection of nanoscale localization in mature cultured neurons and demonstrated similar topology and quantification estimates with PAGFP and mEos. PALM topology assessments of the sodium pump appeared similar to previous STED studies, though quantification estimates varied, implying that labeling strategies, sample analysis and choice of nanoscopic imaging method can be critical factors for correct molecular quantification.