Sequential closure of the cytoplasm and then the periplasm during cell division in Escherichia coli.

Skoog K, Söderström B, Widengren J, von Heijne G, Daley DO

J. Bacteriol. 194 (3) 584-586 [2012-02-00; online 2011-11-22]

To visualize the latter stages of cell division in live Escherichia coli, we have carried out fluorescence recovery after photobleaching (FRAP) on 121 cells expressing cytoplasmic green fluorescent protein and periplasmic mCherry. Our data show conclusively that the cytoplasm is sealed prior to the periplasm during the division event.

Integrated Microscopy Technologies Stockholm [Collaborative]

PubMed 22101847

DOI 10.1128/JB.06091-11

Crossref 10.1128/JB.06091-11

pii: JB.06091-11
pmc: PMC3264074