{"entity": "researcher", "timestamp": "2026-07-11T05:44:32.553Z", "family": "Huber", "given": "Thomas", "initials": "T", "orcid": "0000-0002-1844-262X", "affiliations": ["Laboratory of Chemical Biology and Signal Transduction, The Rockefeller University, 1230 York Ave., New York, NY 10065, USA."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/f730be20f6dd4b8db1756bfed4aa1170.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/f730be20f6dd4b8db1756bfed4aa1170"}}, "publications": [{"entity": "publication", "iuid": "c4121567e5bb46d5bfee7bf4443780a2", "links": {"self": {"href": "https://publications.scilifelab.se/publication/c4121567e5bb46d5bfee7bf4443780a2.json"}, "display": {"href": "https://publications.scilifelab.se/publication/c4121567e5bb46d5bfee7bf4443780a2"}}, "title": "Multiplexed analysis of the secretin-like GPCR-RAMP interactome.", "authors": [{"family": "Lorenzen", "given": "Emily", "initials": "E", "orcid": "0000-0002-0008-0966", "researcher": {"href": "https://publications.scilifelab.se/researcher/e009a3686e804b808372ef5cd3eabaf3.json"}}, {"family": "Dodig-Crnkovi\u0107", "given": "Tea", "initials": "T", "orcid": "0000-0002-2875-896X", "researcher": {"href": "https://publications.scilifelab.se/researcher/cf18af5b676b449693945249fc1767e4.json"}}, {"family": "Kotliar", "given": "Ilana B", "initials": "IB", "orcid": "0000-0001-9533-4828", "researcher": {"href": "https://publications.scilifelab.se/researcher/ddf97aaf7767453a9a165e6198fda679.json"}}, {"family": "Pin", "given": "Elisa", "initials": "E", "orcid": "0000-0002-2158-2674", "researcher": {"href": "https://publications.scilifelab.se/researcher/ccb4db02b9784587b62020716ab87247.json"}}, {"family": "Ceraudo", "given": "Emilie", "initials": "E", "orcid": "0000-0001-6563-9751", "researcher": {"href": "https://publications.scilifelab.se/researcher/58ba695a85a84fe2a7986ea3f3eff4ab.json"}}, {"family": "Vaughan", "given": "Roger D", "initials": "RD"}, {"family": "Uhl\u00e8n", "given": "Mathias", "initials": "M", "orcid": "0000-0002-4858-8056", "researcher": {"href": "https://publications.scilifelab.se/researcher/ff81da3cb0cf4262873b993a1b06798c.json"}}, {"family": "Huber", "given": "Thomas", "initials": "T", "orcid": "0000-0002-1844-262X", "researcher": {"href": "https://publications.scilifelab.se/researcher/f730be20f6dd4b8db1756bfed4aa1170.json"}}, {"family": "Schwenk", "given": "Jochen M", "initials": "JM", "orcid": "0000-0001-8141-8449", "researcher": {"href": "https://publications.scilifelab.se/researcher/aba5822711b246b397fffacb7ae403b3.json"}}, {"family": "Sakmar", "given": "Thomas P", "initials": "TP", "orcid": "0000-0002-2836-8953", "researcher": {"href": "https://publications.scilifelab.se/researcher/1130157044664f8b994b94e3b199cc30.json"}}], "type": "journal article", "published": "2019-09-00", "journal": {"title": "Sci Adv", "issn": "2375-2548", "volume": "5", "issue": "9", "pages": "eaaw2778", "issn-l": "2375-2548"}, "abstract": "Receptor activity-modifying proteins (RAMPs) have been shown to modulate the functions of several G protein-coupled receptors (GPCRs), but potential direct interactions among the three known RAMPs and hundreds of GPCRs have never been investigated. Focusing mainly on the secretin-like family of GPCRs, we engineered epitope-tagged GPCRs and RAMPs, and developed a multiplexed suspension bead array (SBA) immunoassay to detect GPCR-RAMP complexes from detergent-solubilized lysates. Using 64 antibodies raised against the native proteins and 4 antibodies targeting the epitope tags, we mapped the interactions among 23 GPCRs and 3 RAMPs. We validated nearly all previously reported secretin-like GPCR-RAMP interactions, and also found previously unidentified RAMP interactions with additional secretin-like GPCRs, chemokine receptors, and orphan receptors. The results provide a complete interactome of secretin-like GPCRs with RAMPs. The SBA strategy will be useful to search for additional GPCR-RAMP complexes and other interacting membrane protein pairs in cell lines and tissues.", "doi": "10.1126/sciadv.aaw2778", "pmid": "31555726", "labels": {"Affinity Proteomics Stockholm": "Collaborative"}, "xrefs": [{"db": "pii", "key": "aaw2778"}, {"db": "pmc", "key": "PMC6750928"}], "notes": [], "created": "2020-01-08T10:24:44.506Z", "modified": "2021-07-08T12:07:33.919Z"}]}