{"entity": "researcher", "timestamp": "2026-07-20T23:09:41.991Z", "family": "Schexnaydre", "given": "Erin", "initials": "E", "orcid": "0000-0002-2993-8647", "affiliations": ["Department of Clinical Microbiology, Ume\u00e5 University, 90185, Ume\u00e5, Sweden.", "The Laboratory for Molecular Infection Medicine Sweden (MIMS), Ume\u00e5 University, 90187, Ume\u00e5, Sweden.", "Wallenberg Centre for Molecular Medicine, Ume\u00e5 University, 90187, Ume\u00e5, Sweden.", "Department of Medical Biochemistry and Biophysics, Ume\u00e5 University, 90187, Ume\u00e5, Sweden.", "Ume\u00e5 Centre for Microbial Research, Ume\u00e5 University, 90187, Ume\u00e5, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/f643b7350ed5404aac418ce43d907f27.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/f643b7350ed5404aac418ce43d907f27"}}, "publications": [{"entity": "publication", "iuid": "b017a77ea50343cb82a59c299292a958", "links": {"self": {"href": "https://publications.scilifelab.se/publication/b017a77ea50343cb82a59c299292a958.json"}, "display": {"href": "https://publications.scilifelab.se/publication/b017a77ea50343cb82a59c299292a958"}}, "title": "Cryo-electron tomography reveals coupled flavivirus replication, budding and maturation.", "authors": [{"family": "Dahmane", "given": "Selma", "initials": "S", "orcid": "0000-0003-4193-1496", "researcher": {"href": "https://publications.scilifelab.se/researcher/78124ba9dbac444aaee032c91c235a88.json"}}, {"family": "Schexnaydre", "given": "Erin", "initials": "E", "orcid": "0000-0002-2993-8647", "researcher": {"href": "https://publications.scilifelab.se/researcher/f643b7350ed5404aac418ce43d907f27.json"}}, {"family": "Zhang", "given": "Jianguo", "initials": "J"}, {"family": "Singh", "given": "Bina K", "initials": "BK"}, {"family": "Rosendal", "given": "Ebba", "initials": "E", "orcid": "0000-0001-8512-0535", "researcher": {"href": "https://publications.scilifelab.se/researcher/dda3f5ae552241eea145e011afdfad20.json"}}, {"family": "Chotiwan", "given": "Nunya", "initials": "N", "orcid": "0000-0003-3214-6605", "researcher": {"href": "https://publications.scilifelab.se/researcher/aba9da05817a4a8ea7037131000d6a7f.json"}}, {"family": "Sharma", "given": "Kiran B", "initials": "KB"}, {"family": "Nilsson", "given": "Emma", "initials": "E", "orcid": "0000-0001-8123-3292", "researcher": {"href": "https://publications.scilifelab.se/researcher/6070627ce8644379afe4a239a23c0f93.json"}}, {"family": "Peters", "given": "Marie B A", "initials": "MBA", "orcid": "0000-0001-8994-0864", "researcher": {"href": "https://publications.scilifelab.se/researcher/df4733590d054742b732c2028a8f5e8a.json"}}, {"family": "Yau", "given": "Wai-Lok", "initials": "WL"}, {"family": "R\u00f6nfeldt", "given": "Sebastian", "initials": "S"}, {"family": "Lundmark", "given": "Richard", "initials": "R", "orcid": "0000-0001-9104-724X", "researcher": {"href": "https://publications.scilifelab.se/researcher/3e1b756caa79468dab0f960e43cd61d3.json"}}, {"family": "Barad", "given": "Benjamin A", "initials": "BA", "orcid": "0000-0002-1016-862X", "researcher": {"href": "https://publications.scilifelab.se/researcher/8a118a58f2f140bab794786ac581d3ac.json"}}, {"family": "Grotjahn", "given": "Danielle A", "initials": "DA", "orcid": "0000-0001-5908-7882", "researcher": {"href": "https://publications.scilifelab.se/researcher/e9c2292eb2294f56bcc068a7a01737a8.json"}}, {"family": "Liese", "given": "Susanne", "initials": "S"}, {"family": "Carlson", "given": "Andreas", "initials": "A", "orcid": "0000-0002-3068-9983", "researcher": {"href": "https://publications.scilifelab.se/researcher/39f4cc3bec4747da984c7c382a8ca5ae.json"}}, {"family": "\u00d6verby", "given": "Anna K", "initials": "AK", "orcid": "0000-0001-6553-0940", "researcher": {"href": "https://publications.scilifelab.se/researcher/506b0e2b2d884f868df73c7663b9ffb7.json"}}, {"family": "Carlson", "given": "Lars-Anders", "initials": "LA", "orcid": "0000-0003-2342-6488", "researcher": {"href": "https://publications.scilifelab.se/researcher/ba0e366ce21e49b48212bbed5a0a7bd1.json"}}], "type": "journal article", "published": "2026-01-20", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "17", "issue": "1", "pages": "828", "issn-l": "2041-1723"}, "abstract": "Flaviviruses replicate their genomes in replication organelles (ROs) formed as bud-like invaginations on the endoplasmic reticulum membrane, which also functions as the site for virion assembly. While this localization is well established, it is not known to what extent viral membrane remodeling, genome replication, virion assembly, and maturation are coordinated. Here, we image tick-borne flavivirus replication in human cells using cryo-electron tomography. We find that the RO membrane bud is shaped by a combination of a curvature-establishing membrane modification and the pressure from intraluminal template RNA. A protein complex at the RO base extends to an adjacent membrane, where immature virus particles bud. Naturally occurring furin site variants determine whether virus particles mature in the immediate vicinity of ROs. We further visualize replication in mouse brain tissue by cryo-electron tomography. Taken together, these findings reveal a close spatial coupling of flavivirus genome replication, budding, and maturation.", "doi": "10.1038/s41467-026-68483-4", "pmid": "41559045", "labels": {"Cryo-EM": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC12824359"}, {"db": "pii", "key": "10.1038/s41467-026-68483-4"}], "notes": [], "created": "2026-06-24T10:27:41.675Z", "modified": "2026-06-24T10:28:01.603Z"}, {"entity": "publication", "iuid": "3365d1fc9ac7482a996bbeb10f42de8e", "links": {"self": {"href": "https://publications.scilifelab.se/publication/3365d1fc9ac7482a996bbeb10f42de8e.json"}, "display": {"href": "https://publications.scilifelab.se/publication/3365d1fc9ac7482a996bbeb10f42de8e"}}, "title": "Cryo-electron tomography reveals coupled flavivirus replication, budding and maturation.", "authors": [{"family": "Dahmane", "given": "Selma", "initials": "S", "orcid": "0000-0003-4193-1496", "researcher": {"href": "https://publications.scilifelab.se/researcher/78124ba9dbac444aaee032c91c235a88.json"}}, {"family": "Schexnaydre", "given": "Erin", "initials": "E", "orcid": "0000-0002-2993-8647", "researcher": {"href": "https://publications.scilifelab.se/researcher/f643b7350ed5404aac418ce43d907f27.json"}}, {"family": "Zhang", "given": "Jianguo", "initials": "J", "orcid": "0000-0002-3784-6515", "researcher": {"href": "https://publications.scilifelab.se/researcher/3575f209f0d74bc1b8be39dd68d51fd3.json"}}, {"family": "Rosendal", "given": "Ebba", "initials": "E", "orcid": "0000-0001-8512-0535", "researcher": {"href": "https://publications.scilifelab.se/researcher/dda3f5ae552241eea145e011afdfad20.json"}}, {"family": "Chotiwan", "given": "Nunya", "initials": "N", "orcid": "0000-0003-3214-6605", "researcher": {"href": "https://publications.scilifelab.se/researcher/aba9da05817a4a8ea7037131000d6a7f.json"}}, {"family": "Singh", "given": "Bina Kumari", "initials": "BK", "orcid": "0000-0002-9500-2759", "researcher": {"href": "https://publications.scilifelab.se/researcher/ee00e03259f0463f84802861542cf940.json"}}, {"family": "Yau", "given": "Wai-Lok", "initials": "WL", "orcid": "0009-0007-0386-3919", "researcher": {"href": "https://publications.scilifelab.se/researcher/2044fb19cb8c4ecb94ddb6676da79a7d.json"}}, {"family": "Lundmark", "given": "Richard", "initials": "R", "orcid": "0000-0001-9104-724X", "researcher": {"href": "https://publications.scilifelab.se/researcher/3e1b756caa79468dab0f960e43cd61d3.json"}}, {"family": "Barad", "given": "Benjamin", "initials": "B", "orcid": "0000-0002-1016-862X", "researcher": {"href": "https://publications.scilifelab.se/researcher/8a118a58f2f140bab794786ac581d3ac.json"}}, {"family": "Grotjahn", "given": "Danielle A", "initials": "DA", "orcid": "0000-0001-5908-7882", "researcher": {"href": "https://publications.scilifelab.se/researcher/e9c2292eb2294f56bcc068a7a01737a8.json"}}, {"family": "Liese", "given": "Susanne", "initials": "S", "orcid": "0000-0001-7420-5488", "researcher": {"href": "https://publications.scilifelab.se/researcher/cb4ed3f41d6946e1a82472041e9a96d6.json"}}, {"family": "Carlson", "given": "Andreas", "initials": "A", "orcid": "0000-0002-3068-9983", "researcher": {"href": "https://publications.scilifelab.se/researcher/39f4cc3bec4747da984c7c382a8ca5ae.json"}}, {"family": "\u00d6verby", "given": "Anna K", "initials": "AK", "orcid": "0000-0001-6553-0940", "researcher": {"href": "https://publications.scilifelab.se/researcher/506b0e2b2d884f868df73c7663b9ffb7.json"}}, {"family": "Carlson", "given": "Lars-Anders", "initials": "LA", "orcid": "0000-0003-2342-6488", "researcher": {"href": "https://publications.scilifelab.se/researcher/ba0e366ce21e49b48212bbed5a0a7bd1.json"}}], "type": "journal article", "published": "2024-10-21", "journal": {"title": "bioRxiv", "issn": "2692-8205", "issn-l": null, "volume": null, "issue": null, "pages": null}, "abstract": "Flaviviruses replicate their genomes in replication organelles (ROs) formed as bud-like invaginations on the endoplasmic reticulum (ER) membrane, which also functions as the site for virion assembly. While this localization is well established, it is not known to what extent viral membrane remodeling, genome replication, virion assembly, and maturation are coordinated. Here, we imaged tick-borne flavivirus replication in human cells using cryo-electron tomography. We find that the RO membrane bud is shaped by a combination of a curvature-establishing coat and the pressure from intraluminal template RNA. A protein complex at the RO base extends to an adjacent membrane, where immature virions bud. Naturally occurring furin site variants determine whether virions mature in the immediate vicinity of ROs. We further visualize replication in mouse brain tissue by cryo-electron tomography. Taken together, these findings reveal a close spatial coupling of flavivirus genome replication, budding, and maturation.", "doi": "10.1101/2024.10.13.618056", "pmid": "39416041", "labels": {"Cryo-EM": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC11482891"}, {"db": "pii", "key": "2024.10.13.618056"}], "notes": [], "created": "2025-10-29T14:50:30.370Z", "modified": "2025-10-29T14:55:33.629Z"}, {"entity": "publication", "iuid": "dfa9a3ae70d6467682de5c56e5f50b63", "links": {"self": {"href": "https://publications.scilifelab.se/publication/dfa9a3ae70d6467682de5c56e5f50b63.json"}, "display": {"href": "https://publications.scilifelab.se/publication/dfa9a3ae70d6467682de5c56e5f50b63"}}, "title": "Type I interferon shapes brain distribution and tropism of tick-borne flavivirus", "authors": [{"family": "Chotiwan", "given": "Nunya", "initials": "N", "orcid": "0000-0003-3214-6605", "researcher": {"href": "https://publications.scilifelab.se/researcher/aba9da05817a4a8ea7037131000d6a7f.json"}}, {"family": "Rosendal", "given": "Ebba", "initials": "E"}, {"family": "Willekens", "given": "Stefanie M A", "initials": "SMA", "orcid": "0000-0003-3445-7829", "researcher": {"href": "https://publications.scilifelab.se/researcher/8c73f555fa4948fcaed3377742873725.json"}}, {"family": "Schexnaydre", "given": "Erin", "initials": "E", "orcid": "0000-0002-2993-8647", "researcher": {"href": "https://publications.scilifelab.se/researcher/f643b7350ed5404aac418ce43d907f27.json"}}, {"family": "Nilsson", "given": "Emma", "initials": "E", "orcid": "0000-0001-8123-3292", "researcher": {"href": "https://publications.scilifelab.se/researcher/6070627ce8644379afe4a239a23c0f93.json"}}, {"family": "Lindqvist", "given": "Richard", "initials": "R"}, {"family": "Hahn", "given": "Max", "initials": "M", "orcid": "0000-0002-0712-8256", "researcher": {"href": "https://publications.scilifelab.se/researcher/563182c6e86b4107adf691eb73fdf31f.json"}}, {"family": "Mihai", "given": "Ionut Sebastian", "initials": "IS"}, {"family": "Morini", "given": "Federico", "initials": "F"}, {"family": "Zhang", "given": "Jianguo", "initials": "J"}, {"family": "Ebel", "given": "Gregory D", "initials": "GD", "orcid": "0000-0002-4458-9959", "researcher": {"href": "https://publications.scilifelab.se/researcher/f22f43706da74da783697a1d11c6d965.json"}}, {"family": "Carlson", "given": "Lars Anders", "initials": "LA", "orcid": "0000-0003-2342-6488", "researcher": {"href": "https://publications.scilifelab.se/researcher/ba0e366ce21e49b48212bbed5a0a7bd1.json"}}, {"family": "Henriksson", "given": "Johan", "initials": "J", "orcid": "0000-0002-7745-2844", "researcher": {"href": "https://publications.scilifelab.se/researcher/44339821900646b3881d4b4dfd09e8d5.json"}}, {"family": "Ahlgren", "given": "Ulf", "initials": "U", "orcid": "0000-0001-5923-8572", "researcher": {"href": "https://publications.scilifelab.se/researcher/373ea8fda2264851aecc0078161884c8.json"}}, {"family": "Marcellino", "given": "Daniel", "initials": "D", "orcid": "0000-0002-4618-7267", "researcher": {"href": "https://publications.scilifelab.se/researcher/e8a6551237324a3f80a39921078d4183.json"}}, {"family": "\u00d6verby", "given": "Anna K", "initials": "AK", "orcid": "0000-0001-6553-0940", "researcher": {"href": "https://publications.scilifelab.se/researcher/506b0e2b2d884f868df73c7663b9ffb7.json"}}], "type": "journal-article", "published": "2023-04-10", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "14", "issue": "1", "issn-l": "2041-1723"}, "abstract": null, "doi": "10.1038/s41467-023-37698-0", "pmid": null, "labels": {"Integrated Microscopy Technologies Ume\u00e5": "Service"}, "xrefs": [], "notes": [], "created": "2024-01-19T14:40:58.557Z", "modified": "2024-01-19T14:40:58.874Z"}]}