{"entity": "researcher", "timestamp": "2026-09-13T05:52:26.667Z", "family": "Widengren", "given": "Jerker", "initials": "J", "orcid": "0000-0003-3200-0374", "affiliations": ["Royal Institute of Technology (KTH), Dept Applied Physics, Albanova Univ Center, 10691 Stockholm, Sweden"], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/f5db653aece8408bb5aff6531edff22c.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/f5db653aece8408bb5aff6531edff22c"}}, "publications": [{"entity": "publication", "iuid": "27c51de324ea427da6d1ca7c145e1cb6", "links": {"self": {"href": "https://publications.scilifelab.se/publication/27c51de324ea427da6d1ca7c145e1cb6.json"}, "display": {"href": "https://publications.scilifelab.se/publication/27c51de324ea427da6d1ca7c145e1cb6"}}, "title": "Near-infrared MINFLUX imaging enabled by suppression of fluorophore blinking.", "authors": [{"family": "Srambickal", "given": "Chinmaya V", "initials": "CV", "orcid": "0009-0005-3541-008X", "researcher": {"href": "https://publications.scilifelab.se/researcher/83d10a808edf4d34a5ee39d5ae20bd49.json"}}, {"family": "Esmaeeli", "given": "Hanie M", "initials": "HM", "orcid": "0000-0003-3806-0255", "researcher": {"href": "https://publications.scilifelab.se/researcher/b00222b6b4f84c9987d4f30e778da180.json"}}, {"family": "Piguet", "given": "Joachim", "initials": "J", "orcid": "0000-0002-4762-4887", "researcher": {"href": "https://publications.scilifelab.se/researcher/3733dec61dc54d91a52d73b853dddc1d.json"}}, {"family": "Reinkensmeier", "given": "Lenny", "initials": "L", "orcid": "0009-0008-8315-6268", "researcher": {"href": "https://publications.scilifelab.se/researcher/5b753b7873924481bbfdb7d03ce42de2.json"}}, {"family": "Siegmund", "given": "Ren\u00e9", "initials": "R", "orcid": "0009-0004-9010-6067", "researcher": {"href": "https://publications.scilifelab.se/researcher/f1ee32987df6479f86e27cc8c79bb52d.json"}}, {"family": "Agostinho", "given": "Ana", "initials": "A", "orcid": "0000-0001-6270-7384", "researcher": {"href": "https://publications.scilifelab.se/researcher/b66ed948cad14e0c9bd35e487a48d330.json"}}, {"family": "Bates", "given": "Mark", "initials": "M", "orcid": "0000-0003-0668-5277", "researcher": {"href": "https://publications.scilifelab.se/researcher/681d6d1b70ad4879b3bb9a1e458d2960.json"}}, {"family": "Egner", "given": "Alexander", "initials": "A", "orcid": "0000-0001-5248-3858", "researcher": {"href": "https://publications.scilifelab.se/researcher/f6b7bdedf9fa4c08b11701f9643d4f9c.json"}}, {"family": "Widengren", "given": "Jerker", "initials": "J", "orcid": "0000-0003-3200-0374", "researcher": {"href": "https://publications.scilifelab.se/researcher/f5db653aece8408bb5aff6531edff22c.json"}}], "type": "journal article", "published": "2025-12-05", "journal": {"title": "Sci Adv", "issn": "2375-2548", "volume": "11", "issue": "49", "pages": "eadw3149", "issn-l": "2375-2548"}, "abstract": "MINimal photon FLUXes (MINFLUX) offers nanometer localization precision, with lower fluorophore requirements than for other super-resolution microscopy (SRM) techniques. Nonetheless, low localization probabilities hamper its application, and use of less bright and photostable fluorophores, including near-infrared (NIR) fluorophores has been difficult to realize. Here, we devised strategies overcoming these limitations. We systematically studied the blinking properties of far-red and NIR cyanine fluorophores, followed by simulations of MINFLUX localizations, over typical time scales (microsecond to 10 milliseconds), sample and excitation conditions for MINFLUX imaging. We identified fluorophore blinking via photoisomerization and photoreduction as the main cause of localization errors, and that use of balanced redox buffers and repetitive excitation beam scans can suppress such errors. Implementing these strategies, we could demonstrate NIR-MINFLUX imaging with nanometer localization precision, thereby also presenting an overall strategy to design optimal sample and excitation conditions, for MINFLUX imaging and for SRM in general.", "doi": "10.1126/sciadv.adw3149", "pmid": "41348895", "labels": {"Integrated Microscopy Technologies Stockholm": "Collaborative"}, "xrefs": [], "notes": [], "created": "2025-12-06T16:23:27.052Z", "modified": "2025-12-06T16:23:28.621Z"}, {"entity": "publication", "iuid": "2dc3c85c6bc743fba142dcfcea319d81", "links": {"self": {"href": "https://publications.scilifelab.se/publication/2dc3c85c6bc743fba142dcfcea319d81.json"}, "display": {"href": "https://publications.scilifelab.se/publication/2dc3c85c6bc743fba142dcfcea319d81"}}, "title": "Photo-physical characterization of high triplet yield brominated fluoresceins by transient state (TRAST) spectroscopy.", "authors": [{"family": "Demirbay", "given": "Baris", "initials": "B", "orcid": "0000-0002-5454-7437", "researcher": {"href": "https://publications.scilifelab.se/researcher/d8350b404f994101958d9c825d2219eb.json"}}, {"family": "Baryshnikov", "given": "Glib", "initials": "G"}, {"family": "Haraldsson", "given": "Martin", "initials": "M"}, {"family": "Piguet", "given": "Joachim", "initials": "J"}, {"family": "\u00c5gren", "given": "Hans", "initials": "H"}, {"family": "Widengren", "given": "Jerker", "initials": "J", "orcid": "0000-0003-3200-0374", "researcher": {"href": "https://publications.scilifelab.se/researcher/f5db653aece8408bb5aff6531edff22c.json"}}], "type": "journal article", "published": "2023-10-03", "journal": {"title": "Methods Appl. Fluoresc.", "issn": "2050-6120", "volume": "11", "issue": "4", "issn-l": "2050-6120"}, "abstract": "Photo-induced dark transient states of fluorophores can pose a problem in fluorescence spectroscopy. However, their typically long lifetimes also make them highly environment sensitive, suggesting fluorophores with prominent dark-state formation yields to be used as microenvironmental sensors in bio-molecular spectroscopy and imaging. In this work, we analyzed the singlet-triplet transitions of fluorescein and three synthesized carboxy-fluorescein derivatives, with one, two or four bromines linked to the anthracence backbone. Using transient state (TRAST) spectroscopy, we found a prominent internal heavy atom (IHA) enhancement of the intersystem crossing (ISC) rates upon bromination, inferred by density functional theory calculations to take place via a higher triplet state, followed by relaxation to the lowest triplet state. A corresponding external heavy atom (EHA) enhancement was found upon adding potassium iodide (KI). Notably, increased KI concentrations still resulted in lowered triplet state buildup in the brominated fluorophores, due to relatively lower enhancements in ISC, than in the triplet decay. Together with an antioxidative effect on the fluorophores, adding KI thus generated a fluorescence enhancement of the brominated fluorophores. By TRAST measurements, analyzing the average fluorescence intensity of fluorescent molecules subject to a systematically varied excitation modulation, dark state transitions within very high triplet yield (>90%) fluorophores can be directly analyzed under biologically relevant conditions. These measurements, not possible by other techniques such as fluorescence correlation spectroscopy, opens for bio-sensing applications based on high triplet yield fluorophores, and for characterization of high triplet yield photodynamic therapy agents, and how they are influenced by IHA and EHA effects.", "doi": "10.1088/2050-6120/acfb59", "pmid": "37726005", "labels": {"Swedish Metabolomics Centre": "Service", "Chemical Biology Consortium Sweden": "Collaborative"}, "xrefs": [], "notes": [], "created": "2023-10-18T18:37:41.723Z", "modified": "2025-10-17T13:04:27.553Z"}, {"entity": "publication", "iuid": "d21fdb60a89943a9ad8a18a57abebba8", "links": {"self": {"href": "https://publications.scilifelab.se/publication/d21fdb60a89943a9ad8a18a57abebba8.json"}, "display": {"href": "https://publications.scilifelab.se/publication/d21fdb60a89943a9ad8a18a57abebba8"}}, "title": "Stimulated Emission Depletion Microscopy.", "authors": [{"family": "Blom", "given": "Hans", "initials": "H", "orcid": "0000-0002-5584-9170", "researcher": {"href": "https://publications.scilifelab.se/researcher/3ce356a74dc84e0ea6af85397f11d869.json"}}, {"family": "Widengren", "given": "Jerker", "initials": "J", "orcid": "0000-0003-3200-0374", "researcher": {"href": "https://publications.scilifelab.se/researcher/f5db653aece8408bb5aff6531edff22c.json"}}], "type": "journal article", "published": "2017-06-14", "journal": {"volume": "117", "issn": "1520-6890", "issue": "11", "pages": "7377-7427", "title": "Chem. Rev.", "issn-l": "0009-2665"}, "abstract": "Despite its short history, diffraction-unlimited fluorescence microscopy techniques have already made a substantial imprint in the biological sciences. In this review, we describe how stimulated emission depletion (STED) imaging originally evolved, how it compares to other optical super-resolution imaging techniques, and what advantages it provides compared to previous golden-standards for biological microscopy, such as diffraction-limited optical microscopy and electron microscopy. We outline the prerequisites for successful STED imaging experiments, emphasizing the equally critical roles of instrumentation, sample preparation, and photophysics, and describe major evolving strategies for how to push the borders of STED imaging even further in life science. Finally, we provide examples of how STED nanoscopy can be applied, within three different fields with particular potential for STED imaging experiments: neuroscience, plasma membrane biophysics, and subcellular clinical diagnostics. In these areas, and in many more, STED imaging can be expected to play an increasingly important role in the future.", "doi": "10.1021/acs.chemrev.6b00653", "pmid": "28262022", "labels": {"Integrated Microscopy Technologies Stockholm": "Technology development"}, "xrefs": [], "notes": [], "created": "2017-10-05T09:07:25.091Z", "modified": "2021-06-21T15:35:57.559Z"}]}