{"entity": "researcher", "timestamp": "2026-08-15T07:17:36.581Z", "family": "Sork", "given": "Helena", "initials": "H", "orcid": "0000-0002-5390-4420", "affiliations": ["Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden. helena.sork@ki.se."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/f5d08dad4f2d4ee0a3e3a8f060383da5.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/f5d08dad4f2d4ee0a3e3a8f060383da5"}}, "publications": [{"entity": "publication", "iuid": "de5f9b658ce74f6096b9c352ea946d5b", "links": {"self": {"href": "https://publications.scilifelab.se/publication/de5f9b658ce74f6096b9c352ea946d5b.json"}, "display": {"href": "https://publications.scilifelab.se/publication/de5f9b658ce74f6096b9c352ea946d5b"}}, "title": "SARS-CoV-2 and HSV-1 Induce Amyloid Aggregation in Human CSF Resulting in Drastic Soluble Protein Depletion.", "authors": [{"family": "Christ", "given": "Wanda", "initials": "W", "orcid": "0000-0003-3886-5248", "researcher": {"href": "https://publications.scilifelab.se/researcher/38fe3a44fdb547b885cce5b6f490b2bc.json"}}, {"family": "Kapell", "given": "Sebastian", "initials": "S", "orcid": "0000-0001-9304-558X", "researcher": {"href": "https://publications.scilifelab.se/researcher/cffd944f88e54ed6a910183ba49ef656.json"}}, {"family": "Sobkowiak", "given": "Michal J", "initials": "MJ", "orcid": "0000-0003-2932-1994", "researcher": {"href": "https://publications.scilifelab.se/researcher/14f1eb990edf472cac56f24d7cae330c.json"}}, {"family": "Mermelekas", "given": "Georgios", "initials": "G"}, {"family": "Evertsson", "given": "Bj\u00f6rn", "initials": "B"}, {"family": "Sork", "given": "Helena", "initials": "H", "orcid": "0000-0002-5390-4420", "researcher": {"href": "https://publications.scilifelab.se/researcher/f5d08dad4f2d4ee0a3e3a8f060383da5.json"}}, {"family": "Saher", "given": "Osama", "initials": "O"}, {"family": "Bazaz", "given": "Safa", "initials": "S"}, {"family": "Gustafsson", "given": "Oskar", "initials": "O"}, {"family": "Cardenas", "given": "Eduardo I", "initials": "EI"}, {"family": "Villa", "given": "Viviana", "initials": "V"}, {"family": "Ricciarelli", "given": "Roberta", "initials": "R"}, {"family": "Sandberg", "given": "Johan K", "initials": "JK", "orcid": "0000-0002-6275-0750", "researcher": {"href": "https://publications.scilifelab.se/researcher/7468c415a46645a3a4c3d28badcff954.json"}}, {"family": "Bergquist", "given": "Jonas", "initials": "J", "orcid": "0000-0002-4597-041X", "researcher": {"href": "https://publications.scilifelab.se/researcher/d745034529f3423abbea230b4e586d20.json"}}, {"family": "Sturchio", "given": "Andrea", "initials": "A"}, {"family": "Svenningsson", "given": "Per", "initials": "P", "orcid": "0000-0001-6727-3802", "researcher": {"href": "https://publications.scilifelab.se/researcher/5199496295334771ba3c5621a83a6f43.json"}}, {"family": "Malm", "given": "Tarja", "initials": "T", "orcid": "0000-0002-9530-7472", "researcher": {"href": "https://publications.scilifelab.se/researcher/c1ab3c9695424308b07c375901a089f4.json"}}, {"family": "Espay", "given": "Alberto J", "initials": "AJ"}, {"family": "Pernemalm", "given": "Maria", "initials": "M", "orcid": "0000-0003-4624-031X", "researcher": {"href": "https://publications.scilifelab.se/researcher/f15f303cb2044cfa81719700137e3603.json"}}, {"family": "Lind\u00e9n", "given": "Anders", "initials": "A"}, {"family": "Klingstr\u00f6m", "given": "Jonas", "initials": "J", "orcid": "0000-0001-9076-1441", "researcher": {"href": "https://publications.scilifelab.se/researcher/95c1b345ae434fb383b7fe6a1d053c80.json"}}, {"family": "El Andaloussi", "given": "Samir", "initials": "S", "orcid": "0000-0003-4468-9113", "researcher": {"href": "https://publications.scilifelab.se/researcher/bd1036a42043441da3e444f4eac58010.json"}}, {"family": "Ezzat", "given": "Kariem", "initials": "K", "orcid": "0000-0003-4186-0675", "researcher": {"href": "https://publications.scilifelab.se/researcher/6f2f1d3d8a5d467c8fc3b95388e4c606.json"}}], "type": "journal article", "published": "2024-11-20", "journal": {"title": "ACS Chem Neurosci", "issn": "1948-7193", "volume": "15", "issue": "22", "pages": "4095-4104", "issn-l": "1948-7193"}, "abstract": "The corona virus (SARS-CoV-2) pandemic and the resulting long-term neurological complications in patients, known as long COVID, have renewed interest in the correlation between viral infections and neurodegenerative brain disorders. While many viruses can reach the central nervous system (CNS) causing acute or chronic infections (such as herpes simplex virus 1, HSV-1), the lack of a clear mechanistic link between viruses and protein aggregation into amyloids, a characteristic of several neurodegenerative diseases, has rendered such a connection elusive. Recently, we showed that viruses can induce aggregation of purified amyloidogenic proteins via the direct physicochemical mechanism of heterogeneous nucleation (HEN). In the current study, we show that the incubation of HSV-1 and SARS-CoV-2 with human cerebrospinal fluid (CSF) leads to the amyloid aggregation of several proteins known to be involved in neurodegenerative diseases, such as APLP1 (amyloid \u03b2 precursor like protein 1), ApoE, clusterin, \u03b12-macroglobulin, PGK-1 (phosphoglycerate kinase 1), ceruloplasmin, nucleolin, 14-3-3, transthyretin, and vitronectin. Importantly, UV-inactivation of SARS-CoV-2 does not affect its ability to induce amyloid aggregation, as amyloid formation is dependent on viral surface catalysis via HEN and not its ability to replicate. Additionally, viral amyloid induction led to a dramatic drop in the soluble protein concentration in the CSF. Our results show that viruses can physically induce amyloid aggregation of proteins in human CSF and result in soluble protein depletion, thus providing a potential mechanism that may account for the association between persistent and latent/reactivating brain infections and neurodegenerative diseases.", "doi": "10.1021/acschemneuro.4c00636", "pmid": "39510798", "labels": {"Bioinformatics Support, Infrastructure and Training": "Collaborative", "Bioinformatics Support and Infrastructure": "Collaborative", "Global Proteomics and Proteogenomics": "Service", "Bioinformatics (NBIS)": "Collaborative"}, "xrefs": [], "notes": [], "created": "2024-11-15T09:02:02.653Z", "modified": "2025-04-07T07:30:58.294Z"}, {"entity": "publication", "iuid": "c5ac8923292c4f80b7f141382febd809", "links": {"self": {"href": "https://publications.scilifelab.se/publication/c5ac8923292c4f80b7f141382febd809.json"}, "display": {"href": "https://publications.scilifelab.se/publication/c5ac8923292c4f80b7f141382febd809"}}, "title": "Heterogeneity and interplay of the extracellular vesicle small RNA transcriptome and proteome.", "authors": [{"family": "Sork", "given": "Helena", "initials": "H", "orcid": "0000-0002-5390-4420", "researcher": {"href": "https://publications.scilifelab.se/researcher/f5d08dad4f2d4ee0a3e3a8f060383da5.json"}}, {"family": "Corso", "given": "Giulia", "initials": "G"}, {"family": "Krjutskov", "given": "Kaarel", "initials": "K"}, {"family": "Johansson", "given": "Henrik J", "initials": "HJ", "orcid": "0000-0003-4729-4205", "researcher": {"href": "https://publications.scilifelab.se/researcher/18aebf211fa640f48a7c8d860c168e5a.json"}}, {"family": "Nordin", "given": "Joel Z", "initials": "JZ"}, {"family": "Wiklander", "given": "Oscar P B", "initials": "OPB"}, {"family": "Lee", "given": "Yi Xin Fiona", "initials": "YXF", "orcid": "0000-0002-9092-1932", "researcher": {"href": "https://publications.scilifelab.se/researcher/619d8471b5704b45bc8a35ee560b47f7.json"}}, {"family": "Westholm", "given": "Jakub Orzechowski", "initials": "JO", "orcid": "0000-0002-6849-6220", "researcher": {"href": "https://publications.scilifelab.se/researcher/161d8b5fb6734b33ad5f5590edbc0cff.json"}}, {"family": "Lehti\u00f6", "given": "Janne", "initials": "J", "orcid": "0000-0002-8100-9562", "researcher": {"href": "https://publications.scilifelab.se/researcher/8406a97bac744a59b1bc951978994581.json"}}, {"family": "Wood", "given": "Matthew J A", "initials": "MJA"}, {"family": "M\u00e4ger", "given": "Imre", "initials": "I"}, {"family": "El Andaloussi", "given": "Samir", "initials": "S"}], "type": "journal article", "published": "2018-07-17", "journal": {"volume": "8", "issn": "2045-2322", "issue": "1", "pages": "10813", "title": "Sci Rep", "issn-l": "2045-2322"}, "abstract": "Extracellular vesicles (EVs) mediate cell-to-cell communication by delivering or displaying macromolecules to their recipient cells. While certain broad-spectrum EV effects reflect their protein cargo composition, others have been attributed to individual EV-loaded molecules such as specific miRNAs. In this work, we have investigated the contents of vesicular cargo using small RNA sequencing of cells and EVs from HEK293T, RD4, C2C12, Neuro2a and C17.2. The majority of RNA content in EVs (49-96%) corresponded to rRNA-, coding- and tRNA fragments, corroborating with our proteomic analysis of HEK293T and C2C12 EVs which showed an enrichment of ribosome and translation-related proteins. On the other hand, the overall proportion of vesicular small RNA was relatively low and variable (2-39%) and mostly comprised of miRNAs and sequences mapping to piRNA loci. Importantly, this is one of the few studies, which systematically links vesicular RNA and protein cargo of vesicles. Our data is particularly useful for future work in unravelling the biological mechanisms underlying vesicular RNA and protein sorting and serves as an important guide in developing EVs as carriers for RNA therapeutics.", "doi": "10.1038/s41598-018-28485-9", "pmid": "30018314", "labels": {"Bioinformatics Support, Infrastructure and Training": "Collaborative", "Bioinformatics Long-term Support WABI": "Collaborative", "National Genomics Infrastructure": "Service", "NGI Stockholm (Genomics Applications)": "Service", "NGI Stockholm (Genomics Production)": "Service", "Global Proteomics and Proteogenomics": "Collaborative", "Bioinformatics Support for Computational Resources": "Service", "Bioinformatics (NBIS)": "Collaborative"}, "xrefs": [{"db": "pii", "key": "10.1038/s41598-018-28485-9"}, {"db": "pmc", "key": "PMC6050237"}], "notes": [], "created": "2018-08-07T08:38:11.195Z", "modified": "2024-01-16T13:48:45.984Z"}]}