{"entity": "researcher", "timestamp": "2026-07-17T08:05:35.326Z", "family": "Niittyl\u00e4", "given": "Totte", "initials": "T", "orcid": "0000-0001-8029-1503", "affiliations": ["Ume\u00e5 Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Ume\u00e5, 90183, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/f2b8823dc6cf44eaa309fcf157b1f28a.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/f2b8823dc6cf44eaa309fcf157b1f28a"}}, "publications": [{"entity": "publication", "iuid": "60643192a0e04fbe8262b3c6e3cfce9e", "links": {"self": {"href": "https://publications.scilifelab.se/publication/60643192a0e04fbe8262b3c6e3cfce9e.json"}, "display": {"href": "https://publications.scilifelab.se/publication/60643192a0e04fbe8262b3c6e3cfce9e"}}, "title": "Ribosome biogenesis in plants requires the nuclear envelope and mitochondria localized OPENER complex.", "authors": [{"family": "Wang", "given": "Wei", "initials": "W", "orcid": "0000-0003-3400-4889", "researcher": {"href": "https://publications.scilifelab.se/researcher/df33096e9d6345469215380ba9b8ff55.json"}}, {"family": "Mahboubi", "given": "Amir", "initials": "A", "orcid": "0000-0002-0660-0555", "researcher": {"href": "https://publications.scilifelab.se/researcher/75a9b8d8749843c2b908b8712e39096e.json"}}, {"family": "Zhu", "given": "Shaochun", "initials": "S", "orcid": "0000-0001-9945-6718", "researcher": {"href": "https://publications.scilifelab.se/researcher/50c472868ea0437bb9a5a1b0a574bc39.json"}}, {"family": "Hanson", "given": "Johannes", "initials": "J", "orcid": "0000-0002-5605-7984", "researcher": {"href": "https://publications.scilifelab.se/researcher/7d9c9973ca994461ad9f5c27b4341ddb.json"}}, {"family": "Mateus", "given": "Andr\u00e9", "initials": "A", "orcid": "0000-0001-6870-0677", "researcher": {"href": "https://publications.scilifelab.se/researcher/d79942eca68f4b2d8c2e72cf258f1213.json"}}, {"family": "Niittyl\u00e4", "given": "Totte", "initials": "T", "orcid": "0000-0001-8029-1503", "researcher": {"href": "https://publications.scilifelab.se/researcher/f2b8823dc6cf44eaa309fcf157b1f28a.json"}}], "type": "journal article", "published": "2025-08-07", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "16", "issue": "1", "pages": "7301", "issn-l": "2041-1723"}, "abstract": "Eukaryotic ribosome biogenesis proceeds from nucleolus to cytosol assisted by various assembly factors. The process is evolutionarily conserved across eukaryotes but differences between the kingdoms are emerging. Here, we describe how the OPENER (OPNR) protein complex is required for 60S ribosome assembly in the model plant Arabidopsis thaliana. The complex is observed on both nuclear envelope and mitochondria, and contains OPNR, OPENER ASSOCIATED PROTEIN 1 (OAP1), OAP2, Cell Division Cycle 48 D (CDC48D) and Calmodulin-interacting protein 111 (CIP111). Depletion of the OPNR complex components results in reproductive lethality and cytoplasmic retention of assembly factors on 60S ribosomes. Subsequent biochemical analyses and structural modelling suggest that OPNR, OAP1 and OAP2 form a claw-like trimer which grabs the ribosome assembly factor RIBOSOMAL PROTEIN L24C (RPL24C) on the pre-60S ribosome. Our results reveal previously unrecognised subcellular complexity of ribosome biogenesis in plants, and point to mitochondria association as a feature to ensure sufficient translational capacity.", "doi": "10.1038/s41467-025-62652-7", "pmid": "40775240", "labels": {"Integrated Microscopy Technologies Ume\u00e5": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC12332008"}, {"db": "pii", "key": "10.1038/s41467-025-62652-7"}], "notes": [], "created": "2025-08-12T07:59:40.338Z", "modified": "2025-08-12T07:59:41.047Z"}, {"entity": "publication", "iuid": "90d04e62a51b4912bd2650b28270675f", "links": {"self": {"href": "https://publications.scilifelab.se/publication/90d04e62a51b4912bd2650b28270675f.json"}, "display": {"href": "https://publications.scilifelab.se/publication/90d04e62a51b4912bd2650b28270675f"}}, "title": "Sucrose synthase determines carbon allocation in developing wood and alters carbon flow at the whole tree level in aspen.", "authors": [{"family": "Dominguez", "given": "Pia Guadalupe", "initials": "PG", "orcid": "0000-0003-0413-2273", "researcher": {"href": "https://publications.scilifelab.se/researcher/7e1be4b0d59547b38cee663257611bb7.json"}}, {"family": "Donev", "given": "Evgeniy", "initials": "E", "orcid": "0000-0002-7279-0481", "researcher": {"href": "https://publications.scilifelab.se/researcher/26467c84e2664b4980a4ed04335860ad.json"}}, {"family": "Derba-Maceluch", "given": "Marta", "initials": "M", "orcid": "0000-0002-8034-3630", "researcher": {"href": "https://publications.scilifelab.se/researcher/a5be452cef9048e19a08fa8043b74329.json"}}, {"family": "B\u00fcnder", "given": "Anne", "initials": "A"}, {"family": "Hedenstr\u00f6m", "given": "Mattias", "initials": "M", "orcid": "0000-0002-0903-6662", "researcher": {"href": "https://publications.scilifelab.se/researcher/2db0f81a369741b0847c6f79746d82aa.json"}}, {"family": "Tom\u00e1\u0161kov\u00e1", "given": "Ivana", "initials": "I", "orcid": "0000-0001-6219-5980", "researcher": {"href": "https://publications.scilifelab.se/researcher/7db4cb913aef4ca9bfb9da3ddf0c0c70.json"}}, {"family": "Mellerowicz", "given": "Ewa J", "initials": "EJ", "orcid": "0000-0001-6817-1031", "researcher": {"href": "https://publications.scilifelab.se/researcher/a9bf45f4790e4360b19ec021469cfad2.json"}}, {"family": "Niittyl\u00e4", "given": "Totte", "initials": "T", "orcid": "0000-0001-8029-1503", "researcher": {"href": "https://publications.scilifelab.se/researcher/f2b8823dc6cf44eaa309fcf157b1f28a.json"}}], "type": "journal article", "published": "2021-01-00", "journal": {"title": "New Phytol.", "issn": "1469-8137", "issn-l": "0028-646X", "volume": "229", "issue": "1", "pages": "186-198"}, "abstract": "Despite the ecological and industrial importance of biomass accumulation in wood, the control of carbon (C) allocation to this tissue and to other tree tissues remain poorly understood. We studied sucrose synthase (SUS) to clarify its role in biomass formation and C metabolism at the whole tree level in hybrid aspen (Populus tremula \u00d7 tremuloides). To this end, we analysed source leaves, phloem, developing wood, and roots of SUSRNAi trees using a combination of metabolite profiling, 13 CO2 pulse labelling experiments, and long-term field experiments. The glasshouse grown SUSRNAi trees exhibited a mild stem phenotype together with a reduction in wood total C. The 13 CO2 pulse labelling experiments showed an alteration in the C flow in all the analysed tissues, indicating that SUS affects C metabolism at the whole tree level. This was confirmed when the SUSRNAi trees were grown in the field over a 5-yr period; their stem height, diameter and biomass were substantially reduced. These results establish that SUS influences C allocation to developing wood, and that it affects C metabolism at the whole tree level.", "doi": "10.1111/nph.16721", "pmid": "32491203", "labels": {"Swedish Metabolomics Centre": null, "Swedish NMR Centre": "Collaborative"}, "xrefs": [], "notes": [], "created": "2021-01-12T14:42:06.482Z", "modified": "2025-10-17T13:03:56.326Z"}, {"entity": "publication", "iuid": "f7aa6c1e8f454320b6257a67e8196a08", "links": {"self": {"href": "https://publications.scilifelab.se/publication/f7aa6c1e8f454320b6257a67e8196a08.json"}, "display": {"href": "https://publications.scilifelab.se/publication/f7aa6c1e8f454320b6257a67e8196a08"}}, "title": "A metabolite roadmap of the wood-forming tissue in Populus tremula.", "authors": [{"family": "Abreu", "given": "Ilka N", "initials": "IN", "orcid": "0000-0003-4728-0161", "researcher": {"href": "https://publications.scilifelab.se/researcher/1cb725d57dfe40588d386dafe2c58479.json"}}, {"family": "Johansson", "given": "Annika I", "initials": "AI", "orcid": "0000-0001-5000-1288", "researcher": {"href": "https://publications.scilifelab.se/researcher/0b0835b94db946929c1cb0c8f9319068.json"}}, {"family": "Soko\u0142owska", "given": "Katarzyna", "initials": "K", "orcid": "0000-0002-5874-207X", "researcher": {"href": "https://publications.scilifelab.se/researcher/13bfc250ddff4dc3a38073d0717f2a45.json"}}, {"family": "Niittyl\u00e4", "given": "Totte", "initials": "T", "orcid": "0000-0001-8029-1503", "researcher": {"href": "https://publications.scilifelab.se/researcher/f2b8823dc6cf44eaa309fcf157b1f28a.json"}}, {"family": "Sundberg", "given": "Bj\u00f6rn", "initials": "B"}, {"family": "Hvidsten", "given": "Torgeir R", "initials": "TR", "orcid": "0000-0001-6097-2539", "researcher": {"href": "https://publications.scilifelab.se/researcher/987fbb5763c74f6895bee64630528d8d.json"}}, {"family": "Street", "given": "Nathaniel R", "initials": "NR", "orcid": "0000-0001-6031-005X", "researcher": {"href": "https://publications.scilifelab.se/researcher/cb9ceb237a724046a1454179a32de1b0.json"}}, {"family": "Moritz", "given": "Thomas", "initials": "T", "orcid": "0000-0002-4258-3190", "researcher": {"href": "https://publications.scilifelab.se/researcher/95ad5b7fe48f42eda1328f54a385e097.json"}}], "type": "journal article", "published": "2020-12-00", "journal": {"title": "New Phytol.", "issn": "1469-8137", "volume": "228", "issue": "5", "pages": "1559-1572", "issn-l": "0028-646X"}, "abstract": "Wood, or secondary xylem, is the product of xylogenesis, a developmental process that begins with the proliferation of cambial derivatives and ends with mature xylem fibers and vessels with lignified secondary cell walls. Fully mature xylem has undergone a series of cellular processes, including cell division, cell expansion, secondary wall formation, lignification and programmed cell death. A complex network of interactions between transcriptional regulators and signal transduction pathways controls wood formation. However, the role of metabolites during this developmental process has not been comprehensively characterized. To evaluate the role of metabolites during wood formation, we performed a high spatial resolution metabolomics study of the wood-forming zone of Populus tremula, including laser dissected aspen ray and fiber cells. We show that metabolites show specific patterns within the wood-forming zone, following the differentiation process from cell division to cell death. The data from profiled laser dissected aspen ray and fiber cells suggests that these two cell types host distinctly different metabolic processes. Furthermore, by integrating previously published transcriptomic and proteomic profiles generated from the same trees, we provide an integrative picture of molecular processes, for example, deamination of phenylalanine during lignification is of critical importance for nitrogen metabolism during wood formation.", "doi": "10.1111/nph.16799", "pmid": "32648607", "labels": {"Bioinformatics Support for Computational Resources": "Service", "Swedish Metabolomics Centre": "Collaborative"}, "xrefs": [], "notes": [], "created": "2020-12-11T11:56:03.232Z", "modified": "2025-10-17T13:03:16.572Z"}, {"entity": "publication", "iuid": "2422b4d991cf4626b7717113f4f2ecc4", "links": {"self": {"href": "https://publications.scilifelab.se/publication/2422b4d991cf4626b7717113f4f2ecc4.json"}, "display": {"href": "https://publications.scilifelab.se/publication/2422b4d991cf4626b7717113f4f2ecc4"}}, "title": "CELLULOSE SYNTHASE INTERACTING 1 is required for wood mechanics and leaf morphology in aspen.", "authors": [{"family": "B\u00fcnder", "given": "Anne", "initials": "A"}, {"family": "Sundman", "given": "Ola", "initials": "O"}, {"family": "Mahboubi", "given": "Amir", "initials": "A"}, {"family": "Persson", "given": "Staffan", "initials": "S"}, {"family": "Mansfield", "given": "Shawn D", "initials": "SD", "orcid": "0000-0002-0175-554X", "researcher": {"href": "https://publications.scilifelab.se/researcher/a6b6ce31a62344e297c43e4a0f647fdd.json"}}, {"family": "R\u00fcggeberg", "given": "Markus", "initials": "M"}, {"family": "Niittyl\u00e4", "given": "Totte", "initials": "T", "orcid": "0000-0001-8029-1503", "researcher": {"href": "https://publications.scilifelab.se/researcher/f2b8823dc6cf44eaa309fcf157b1f28a.json"}}], "type": "journal article", "published": "2020-08-00", "journal": {"title": "Plant J.", "issn": "1365-313X", "issn-l": "0960-7412", "volume": "103", "issue": "5", "pages": "1858-1868"}, "abstract": "Cellulose microfibrils synthesized by CELLULOSE SYNTHASE COMPLEXES (CSCs) are the main load-bearing polymers in wood. CELLULOSE SYNTHASE INTERACTING1 (CSI1) connects CSCs with cortical microtubules, which align with cellulose microfibrils. Mechanical properties of wood are dependent on cellulose microfibril alignment and structure in the cell walls, but the molecular mechanism(s) defining these features is unknown. Herein, we investigated the role of CSI1 in hybrid aspen (Populus tremula \u00d7 Populus tremuloides) by characterizing transgenic lines with significantly reduced CSI1 transcript abundance. Reduction in leaves (50-80%) caused leaf twisting and misshaped pavement cells, while reduction (70-90%) in developing xylem led to impaired mechanical wood properties evident as a decrease in the elastic modulus and rupture. X-ray diffraction measurements indicate that microfibril angle was not impacted by the altered CSI1 abundance in developing wood fibres. Instead, the augmented wood phenotype of the transgenic trees was associated with a reduced cellulose degree of polymerization. These findings establish a function for CSI1 in wood mechanics and in defining leaf cell shape. Furthermore, the results imply that the microfibril angle in wood is defined by CSI1 independent mechanism(s).", "doi": "10.1111/tpj.14873", "pmid": "32526794", "labels": {"Integrated Microscopy Technologies Ume\u00e5": "Service"}, "xrefs": [], "notes": [], "created": "2022-04-01T15:03:35.012Z", "modified": "2022-04-01T15:03:48.167Z"}, {"entity": "publication", "iuid": "92c43c9fd60b4caea6f76728ab06de33", "links": {"self": {"href": "https://publications.scilifelab.se/publication/92c43c9fd60b4caea6f76728ab06de33.json"}, "display": {"href": "https://publications.scilifelab.se/publication/92c43c9fd60b4caea6f76728ab06de33"}}, "title": "OPENER Is a Nuclear Envelope and Mitochondria Localized Protein Required for Cell Cycle Progression in Arabidopsis.", "authors": [{"family": "Wang", "given": "Wei", "initials": "W", "orcid": "0000-0003-3400-4889", "researcher": {"href": "https://publications.scilifelab.se/researcher/df33096e9d6345469215380ba9b8ff55.json"}}, {"family": "Zhang", "given": "Xueyang", "initials": "X", "orcid": "0000-0002-3942-8333", "researcher": {"href": "https://publications.scilifelab.se/researcher/620dd725699843bbb10eb31b46b7b9c0.json"}}, {"family": "Niittyl\u00e4", "given": "Totte", "initials": "T", "orcid": "0000-0001-8029-1503", "researcher": {"href": "https://publications.scilifelab.se/researcher/f2b8823dc6cf44eaa309fcf157b1f28a.json"}}], "type": "journal article", "published": "2019-07-00", "journal": {"title": "Plant Cell", "issn": "1532-298X", "issn-l": "1040-4651", "volume": "31", "issue": "7", "pages": "1446-1465"}, "abstract": "Currently one-third of the proteins encoded by the Arabidopsis ( Arabidopsis thaliana) genome are of unknown function. Some of these unknown proteins are likely to be involved in uncharacterized vital biological processes. Evolutionarily conserved single copy genes in flowering plants have been shown to be enriched in essential housekeeping functions. This together with publicly available gene expression data allows for a focused search for uncharacterized essential genes. Here we identify an essential single copy gene called OPENER (OPNR) in Arabidopsis. We show that OPNR is predominantly expressed in actively dividing cells and performs essential functions in seed development and root meristem maintenance. Cell cycle tracking using 5-ethynyl-2'-deoxyuridine staining and fluorescent cell cycle markers together with the increased size of nucleolus and nucleus in opnr mutants indicate that OPNR is required for cell cycle progression through the S or G2 phases. Intriguingly, OPNR localizes to the nuclear envelope and mitochondria. Furthermore, the nuclear envelope localization of OPNR is dependent on its interaction with nuclear inner membrane Sad1/UNC-84 (SUN) domain proteins SUN1 and SUN2. Taken together our results open a line of investigation into an evolutionarily conserved essential cellular process occurring in both the nuclear envelopes and mitochondria of dividing cells.", "doi": "10.1105/tpc.19.00033", "pmid": "31023726", "labels": {"Integrated Microscopy Technologies Ume\u00e5": "Service"}, "xrefs": [{"db": "pii", "key": "tpc.19.00033"}, {"db": "pmc", "key": "PMC6635878"}], "notes": [], "created": "2020-01-08T09:06:36.159Z", "modified": "2022-04-01T15:15:34.409Z"}]}