{"entity": "researcher", "timestamp": "2026-07-11T15:02:39.075Z", "family": "H\u00f6\u00f6g", "given": "Johanna L", "initials": "JL", "orcid": "0000-0003-2162-3816", "affiliations": ["Department of Chemistry and Molecular Biology University of Gothenburg Gothenburg Sweden"], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/f1eaedff964f4060ae6e69f59cad4521.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/f1eaedff964f4060ae6e69f59cad4521"}}, "publications": [{"entity": "publication", "iuid": "388fe736e91941cd9ccb66a502243168", "links": {"self": {"href": "https://publications.scilifelab.se/publication/388fe736e91941cd9ccb66a502243168.json"}, "display": {"href": "https://publications.scilifelab.se/publication/388fe736e91941cd9ccb66a502243168"}}, "title": "Intracellular Regulation of a Serotonin-Gated Ion Channel Links Receptor Trafficking to Memory", "authors": [{"family": "Cesar", "given": "Leona", "initials": "L", "orcid": "0000-0002-4480-2000", "researcher": {"href": "https://publications.scilifelab.se/researcher/18e4da37bd9c45bb998d2f1f20af2036.json"}}, {"family": "Zabeo", "given": "Davide", "initials": "D", "orcid": "0000-0002-5912-4601", "researcher": {"href": "https://publications.scilifelab.se/researcher/03943200c1ae493ab8b7d6886d0af356.json"}}, {"family": "Aspholm", "given": "Emelie", "initials": "E", "orcid": "0000-0001-5878-0722", "researcher": {"href": "https://publications.scilifelab.se/researcher/45811e98fea84e979a0f75adac508749.json"}}, {"family": "Panagaki", "given": "Dimitra", "initials": "D"}, {"family": "H\u00f6\u00f6g", "given": "Johanna Louise", "initials": "JL", "orcid": "0000-0003-2162-3816", "researcher": {"href": "https://publications.scilifelab.se/researcher/f1eaedff964f4060ae6e69f59cad4521.json"}}, {"family": "Morud", "given": "Julia", "initials": "J", "orcid": "0000-0003-1925-5938", "researcher": {"href": "https://publications.scilifelab.se/researcher/d41dc497590f451994c8164a67045c6c.json"}}], "type": "posted-content", "published": "2025-10-27", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2025.10.27.684756", "pmid": null, "labels": {"Integrated Microscopy Technologies Gothenburg": "Service"}, "xrefs": [], "notes": [], "created": "2026-01-12T10:07:21.362Z", "modified": "2026-01-12T10:07:21.740Z"}, {"entity": "publication", "iuid": "5e2eb91347d34701b54d4c5dbe67d2c1", "links": {"self": {"href": "https://publications.scilifelab.se/publication/5e2eb91347d34701b54d4c5dbe67d2c1.json"}, "display": {"href": "https://publications.scilifelab.se/publication/5e2eb91347d34701b54d4c5dbe67d2c1"}}, "title": "Nuclear Hsp104 safeguards the dormant translation machinery during quiescence.", "authors": [{"family": "Kohler", "given": "Verena", "initials": "V", "orcid": "0000-0002-1241-162X", "researcher": {"href": "https://publications.scilifelab.se/researcher/a12e1c1d4e71468c99fa454c7c49b72b.json"}}, {"family": "Kohler", "given": "Andreas", "initials": "A", "orcid": "0000-0001-6571-2162", "researcher": {"href": "https://publications.scilifelab.se/researcher/d8159b879db1414f9583ce2b3184cd23.json"}}, {"family": "Berglund", "given": "Lisa Larsson", "initials": "LL"}, {"family": "Hao", "given": "Xinxin", "initials": "X", "orcid": "0000-0001-5758-6290", "researcher": {"href": "https://publications.scilifelab.se/researcher/36931f6141fe452bad02e5f576fca10e.json"}}, {"family": "Gersing", "given": "Sarah", "initials": "S"}, {"family": "Imhof", "given": "Axel", "initials": "A", "orcid": "0000-0003-2993-8249", "researcher": {"href": "https://publications.scilifelab.se/researcher/d0e2b7576e984458839c000e40c0900b.json"}}, {"family": "Nystr\u00f6m", "given": "Thomas", "initials": "T", "orcid": "0000-0001-5489-2903", "researcher": {"href": "https://publications.scilifelab.se/researcher/de24db2a24cd451db3b8e6151957601d.json"}}, {"family": "H\u00f6\u00f6g", "given": "Johanna L", "initials": "JL", "orcid": "0000-0003-2162-3816", "researcher": {"href": "https://publications.scilifelab.se/researcher/f1eaedff964f4060ae6e69f59cad4521.json"}}, {"family": "Ott", "given": "Martin", "initials": "M"}, {"family": "Andr\u00e9asson", "given": "Claes", "initials": "C", "orcid": "0000-0001-8948-0685", "researcher": {"href": "https://publications.scilifelab.se/researcher/c4cf1de8575d409b821467bf515c3af6.json"}}, {"family": "B\u00fcttner", "given": "Sabrina", "initials": "S", "orcid": "0000-0002-2786-8542", "researcher": {"href": "https://publications.scilifelab.se/researcher/6be7c476049b4f3497d187cba3daad77.json"}}], "type": "journal article", "published": "2024-01-05", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "15", "issue": "1", "pages": "315", "issn-l": "2041-1723"}, "abstract": "The resilience of cellular proteostasis declines with age, which drives protein aggregation and compromises viability. The nucleus has emerged as a key quality control compartment that handles misfolded proteins produced by the cytosolic protein biosynthesis system. Here, we find that age-associated metabolic cues target the yeast protein disaggregase Hsp104 to the nucleus to maintain a functional nuclear proteome during quiescence. The switch to respiratory metabolism and the accompanying decrease in translation rates direct cytosolic Hsp104 to the nucleus to interact with latent translation initiation factor eIF2 and to suppress protein aggregation. Hindering Hsp104 from entering the nucleus in quiescent cells results in delayed re-entry into the cell cycle due to compromised resumption of protein synthesis. In sum, we report that cytosolic-nuclear partitioning of the Hsp104 disaggregase is a critical mechanism to protect the latent protein synthesis machinery during quiescence in yeast, ensuring the rapid restart of translation once nutrients are replenished.", "doi": "10.1038/s41467-023-44538-8", "pmid": "38182580", "labels": {"Global Proteomics and Proteogenomics": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC10770042"}, {"db": "pii", "key": "10.1038/s41467-023-44538-8"}], "notes": [], "created": "2024-01-09T12:59:47.170Z", "modified": "2024-11-27T13:10:41.903Z"}, {"entity": "publication", "iuid": "452f9e423170414ea771ee32215c63e1", "links": {"self": {"href": "https://publications.scilifelab.se/publication/452f9e423170414ea771ee32215c63e1.json"}, "display": {"href": "https://publications.scilifelab.se/publication/452f9e423170414ea771ee32215c63e1"}}, "title": "Sphingosine 1-phosphate mediates adiponectin receptor signaling essential for lipid homeostasis and embryogenesis.", "authors": [{"family": "Ruiz", "given": "Mario", "initials": "M", "orcid": "0000-0002-7149-6600", "researcher": {"href": "https://publications.scilifelab.se/researcher/35ea5372ccf64bad864d90e4b3698a24.json"}}, {"family": "Devkota", "given": "Ranjan", "initials": "R"}, {"family": "Panagaki", "given": "Dimitra", "initials": "D"}, {"family": "Bergh", "given": "Per-Olof", "initials": "PO", "orcid": "0000-0001-9993-6965", "researcher": {"href": "https://publications.scilifelab.se/researcher/28c1f37dc6cc4ed98c8e8eb1a621fa2c.json"}}, {"family": "Kaper", "given": "Delaney", "initials": "D", "orcid": "0000-0002-9929-8104", "researcher": {"href": "https://publications.scilifelab.se/researcher/e96d29226c884ec496240844de56499a.json"}}, {"family": "Henricsson", "given": "Marcus", "initials": "M", "orcid": "0000-0002-4202-0339", "researcher": {"href": "https://publications.scilifelab.se/researcher/01a323cbf0a24269bd32bfc34539e021.json"}}, {"family": "Nik", "given": "Ali", "initials": "A"}, {"family": "Petkevicius", "given": "Kasparas", "initials": "K", "orcid": "0000-0003-2295-6065", "researcher": {"href": "https://publications.scilifelab.se/researcher/d807cea22e324d8297776e90d31ed636.json"}}, {"family": "H\u00f6\u00f6g", "given": "Johanna L", "initials": "JL", "orcid": "0000-0003-2162-3816", "researcher": {"href": "https://publications.scilifelab.se/researcher/f1eaedff964f4060ae6e69f59cad4521.json"}}, {"family": "Bohlooly-Y", "given": "Mohammad", "initials": "M"}, {"family": "Carlsson", "given": "Peter", "initials": "P"}, {"family": "Bor\u00e9n", "given": "Jan", "initials": "J", "orcid": "0000-0003-0786-8091", "researcher": {"href": "https://publications.scilifelab.se/researcher/1e85f6d287ce4c60a7b35b287efb4f79.json"}}, {"family": "Pilon", "given": "Marc", "initials": "M", "orcid": "0000-0003-3919-2882", "researcher": {"href": "https://publications.scilifelab.se/researcher/d45c4ecf9afe463c971af2de53a770a8.json"}}], "type": "journal article", "published": "2022-11-22", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "13", "issue": "1", "pages": "7162", "issn-l": "2041-1723"}, "abstract": "Cells and organisms require proper membrane composition to function and develop. Phospholipids are the major component of membranes and are primarily acquired through the diet. Given great variability in diet composition, cells must be able to deploy mechanisms that correct deviations from optimal membrane composition and properties. Here, using lipidomics and unbiased proteomics, we found that the embryonic lethality in mice lacking the fluidity regulators Adiponectin Receptors 1 and 2 (AdipoR1/2) is associated with aberrant high saturation of the membrane phospholipids. Using mouse embryonic fibroblasts (MEFs) derived from AdipoR1/2-KO embryos, human cell lines and the model organism C. elegans we found that, mechanistically, AdipoR1/2-derived sphingosine 1-phosphate (S1P) signals in parallel through S1PR3-SREBP1 and PPAR\u03b3 to sustain the expression of the fatty acid desaturase SCD and maintain membrane properties. Thus, our work identifies an evolutionary conserved pathway by which cells and organisms achieve membrane homeostasis and adapt to a variable environment.", "doi": "10.1038/s41467-022-34931-0", "pmid": "36418331", "labels": {"Glycoproteomics and MS Proteomics": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC9684441"}, {"db": "pii", "key": "10.1038/s41467-022-34931-0"}], "notes": [], "created": "2023-03-07T14:34:55.175Z", "modified": "2024-01-16T13:46:28.029Z"}, {"entity": "publication", "iuid": "1ad8c2dd0dfa48ab82d657abd2bde56c", "links": {"self": {"href": "https://publications.scilifelab.se/publication/1ad8c2dd0dfa48ab82d657abd2bde56c.json"}, "display": {"href": "https://publications.scilifelab.se/publication/1ad8c2dd0dfa48ab82d657abd2bde56c"}}, "title": "SPACA9 is a lumenal protein of human ciliary singlet and doublet microtubules.", "authors": [{"family": "Gui", "given": "Miao", "initials": "M", "orcid": "0000-0001-9113-8029", "researcher": {"href": "https://publications.scilifelab.se/researcher/2450958ede4a4f3695554ffef59f3315.json"}}, {"family": "Croft", "given": "Jacob T", "initials": "JT", "orcid": "0000-0002-8964-3699", "researcher": {"href": "https://publications.scilifelab.se/researcher/a9eeccd7d00e4b8a8bca946fc5851a01.json"}}, {"family": "Zabeo", "given": "Davide", "initials": "D", "orcid": "0000-0002-5912-4601", "researcher": {"href": "https://publications.scilifelab.se/researcher/03943200c1ae493ab8b7d6886d0af356.json"}}, {"family": "Acharya", "given": "Vajradhar", "initials": "V"}, {"family": "Kollman", "given": "Justin M", "initials": "JM"}, {"family": "Burgoyne", "given": "Thomas", "initials": "T", "orcid": "0000-0002-8428-720X", "researcher": {"href": "https://publications.scilifelab.se/researcher/2a020f564d2646709c19e4526756f84e.json"}}, {"family": "H\u00f6\u00f6g", "given": "Johanna L", "initials": "JL", "orcid": "0000-0003-2162-3816", "researcher": {"href": "https://publications.scilifelab.se/researcher/f1eaedff964f4060ae6e69f59cad4521.json"}}, {"family": "Brown", "given": "Alan", "initials": "A", "orcid": "0000-0002-0021-0476", "researcher": {"href": "https://publications.scilifelab.se/researcher/2bb38a7f086247fdb2e2b3bfb80dae1e.json"}}], "type": "journal article", "published": "2022-10-11", "journal": {"title": "Proc. Natl. Acad. Sci. U.S.A.", "issn": "1091-6490", "volume": "119", "issue": "41", "pages": "e2207605119", "issn-l": "0027-8424"}, "abstract": "The cilium-centrosome complex contains triplet, doublet, and singlet microtubules. The lumenal surfaces of each microtubule within this diverse array are decorated by microtubule inner proteins (MIPs). Here, we used single-particle cryo-electron microscopy methods to build atomic models of two types of human ciliary microtubule: the doublet microtubules of multiciliated respiratory cells and the distal singlet microtubules of monoflagellated human spermatozoa. We discover that SPACA9 is a polyspecific MIP capable of binding both microtubule types. SPACA9 forms intralumenal striations in the B tubule of respiratory doublet microtubules and noncontinuous spirals in sperm singlet microtubules. By acquiring new and reanalyzing previous cryo-electron tomography data, we show that SPACA9-like intralumenal striations are common features of different microtubule types in animal cilia. Our structures provide detailed references to help rationalize ciliopathy-causing mutations and position cryo-EM as a tool for the analysis of samples obtained directly from ciliopathy patients.", "doi": "10.1073/pnas.2207605119", "pmid": "36191189", "labels": {"Cryo-EM": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC9564825"}], "notes": [], "created": "2022-12-06T11:33:00.809Z", "modified": "2022-12-06T11:33:00.918Z"}, {"entity": "publication", "iuid": "e85aac1ccfe44c689708ad7b122acfa4", "links": {"self": {"href": "https://publications.scilifelab.se/publication/e85aac1ccfe44c689708ad7b122acfa4.json"}, "display": {"href": "https://publications.scilifelab.se/publication/e85aac1ccfe44c689708ad7b122acfa4"}}, "title": "Genetically controlled mtDNA deletions prevent ROS damage by arresting oxidative phosphorylation.", "authors": [{"family": "Stenberg", "given": "Simon", "initials": "S", "orcid": "0000-0003-0300-1730", "researcher": {"href": "https://publications.scilifelab.se/researcher/d59ba977fddf4071928bca5fdfc04ba2.json"}}, {"family": "Li", "given": "Jing", "initials": "J"}, {"family": "Gjuvsland", "given": "Arne B", "initials": "AB"}, {"family": "Persson", "given": "Karl", "initials": "K"}, {"family": "Demitz-Helin", "given": "Erik", "initials": "E"}, {"family": "Gonz\u00e1lez Pe\u00f1a", "given": "Carles", "initials": "C", "orcid": "0000-0002-7771-7988", "researcher": {"href": "https://publications.scilifelab.se/researcher/e92cd4ba564e4d0999681bf59cc95c70.json"}}, {"family": "Yue", "given": "Jia-Xing", "initials": "JX", "orcid": "0000-0002-2122-9221", "researcher": {"href": "https://publications.scilifelab.se/researcher/9c17dbcd3dff4f61aed7cc85a3a7136a.json"}}, {"family": "Gilchrist", "given": "Ciaran", "initials": "C"}, {"family": "\u00c4reng\u00e5rd", "given": "Timmy", "initials": "T"}, {"family": "Ghiaci", "given": "Payam", "initials": "P"}, {"family": "Larsson-Berglund", "given": "Lisa", "initials": "L"}, {"family": "Zackrisson", "given": "Martin", "initials": "M"}, {"family": "Smits", "given": "Silvana", "initials": "S"}, {"family": "Hallin", "given": "Johan", "initials": "J"}, {"family": "H\u00f6\u00f6g", "given": "Johanna L", "initials": "JL", "orcid": "0000-0003-2162-3816", "researcher": {"href": "https://publications.scilifelab.se/researcher/f1eaedff964f4060ae6e69f59cad4521.json"}}, {"family": "Molin", "given": "Mikael", "initials": "M", "orcid": "0000-0002-3903-8503", "researcher": {"href": "https://publications.scilifelab.se/researcher/12e9dc11d1b047d88913ea3bcb2694be.json"}}, {"family": "Liti", "given": "Gianni", "initials": "G", "orcid": "0000-0002-2318-0775", "researcher": {"href": "https://publications.scilifelab.se/researcher/96fb0b05418743bc9966610ff45a3220.json"}}, {"family": "Omholt", "given": "Stig W", "initials": "SW", "orcid": "0000-0002-8320-4337", "researcher": {"href": "https://publications.scilifelab.se/researcher/8a0f2458dfa64ac09c95356b1baf9f55.json"}}, {"family": "Warringer", "given": "Jonas", "initials": "J", "orcid": "0000-0001-6144-2740", "researcher": {"href": "https://publications.scilifelab.se/researcher/864cb0fde85a4aaeb68627f67e97d283.json"}}], "type": "journal article", "published": "2022-07-08", "journal": {"title": "Elife", "issn": "2050-084X", "volume": "11", "issn-l": "2050-084X"}, "abstract": "Deletion of mitochondrial DNA in eukaryotes is currently attributed to rare accidental events associated with mitochondrial replication or repair of double-strand breaks. We report the discovery that yeast cells arrest harmful intramitochondrial superoxide production by shutting down respiration through genetically controlled deletion of mitochondrial oxidative phosphorylation genes. We show that this process critically involves the antioxidant enzyme superoxide dismutase 2 and two-way mitochondrial-nuclear communication through Rtg2 and Rtg3. While mitochondrial DNA homeostasis is rapidly restored after cessation of a short-term superoxide stress, long-term stress causes maladaptive persistence of the deletion process, leading to complete annihilation of the cellular pool of intact mitochondrial genomes and irrevocable loss of respiratory ability. This shows that oxidative stress-induced mitochondrial impairment may be under strict regulatory control. If the results extend to human cells, the results may prove to be of etiological as well as therapeutic importance with regard to age-related mitochondrial impairment and disease.", "doi": "10.7554/eLife.76095", "pmid": "35801695", "labels": {"Integrated Microscopy Technologies Gothenburg": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC9427111"}, {"db": "pii", "key": "76095"}], "notes": [], "created": "2023-02-16T08:26:04.422Z", "modified": "2023-02-16T08:26:04.606Z"}, {"entity": "publication", "iuid": "45731fef896f4c55814ee2668776f539", "links": {"self": {"href": "https://publications.scilifelab.se/publication/45731fef896f4c55814ee2668776f539.json"}, "display": {"href": "https://publications.scilifelab.se/publication/45731fef896f4c55814ee2668776f539"}}, "title": "Subpopulations of extracellular vesicles from human metastatic melanoma tissue identified by quantitative proteomics after optimized isolation.", "authors": [{"family": "Crescitelli", "given": "Rossella", "initials": "R", "orcid": "0000-0002-1714-3169", "researcher": {"href": "https://publications.scilifelab.se/researcher/1d4b955f26ca4a679deb090e30d0ccf1.json"}}, {"family": "L\u00e4sser", "given": "Cecilia", "initials": "C", "orcid": "0000-0003-1279-1746", "researcher": {"href": "https://publications.scilifelab.se/researcher/e14e17d2cdb24a9f93991d83811c78b9.json"}}, {"family": "Jang", "given": "Su Chul", "initials": "SC", "orcid": "0000-0003-3326-1007", "researcher": {"href": "https://publications.scilifelab.se/researcher/d7866f9f05f74587af9a4d5beec0a1b5.json"}}, {"family": "Cvjetkovic", "given": "Aleksander", "initials": "A", "orcid": "0000-0002-9131-9791", "researcher": {"href": "https://publications.scilifelab.se/researcher/166451e5c8e54c0da297f6238c42c334.json"}}, {"family": "Malmh\u00e4ll", "given": "Carina", "initials": "C"}, {"family": "Karimi", "given": "Nasibeh", "initials": "N"}, {"family": "H\u00f6\u00f6g", "given": "Johanna L", "initials": "JL", "orcid": "0000-0003-2162-3816", "researcher": {"href": "https://publications.scilifelab.se/researcher/f1eaedff964f4060ae6e69f59cad4521.json"}}, {"family": "Johansson", "given": "Iva", "initials": "I", "orcid": "0000-0003-2162-3816", "researcher": {"href": "https://publications.scilifelab.se/researcher/f1eaedff964f4060ae6e69f59cad4521.json"}}, {"family": "Fuchs", "given": "Johannes", "initials": "J"}, {"family": "Thorsell", "given": "Annika", "initials": "A"}, {"family": "Gho", "given": "Yong Song", "initials": "YS"}, {"family": "Olofsson Bagge", "given": "R", "initials": "R", "orcid": "0000-0001-5795-0355", "researcher": {"href": "https://publications.scilifelab.se/researcher/e1f8016d5e64418ca71f230e64e415ba.json"}}, {"family": "L\u00f6tvall", "given": "Jan", "initials": "J", "orcid": "0000-0001-9195-9249", "researcher": {"href": "https://publications.scilifelab.se/researcher/0cbcaf6b5698411c92e0de9e8fcf390f.json"}}], "type": "journal article", "published": "2020-02-11", "journal": {"title": "J Extracell Vesicles", "issn": "2001-3078", "volume": "9", "issue": "1", "pages": "1722433", "issn-l": "2001-3078"}, "abstract": "The majority of extracellular vesicle (EV) studies conducted to date have been performed on cell lines with little knowledge on how well these represent the characteristics of EVs in vivo. The aim of this study was to establish a method to isolate and categorize subpopulations of EVs isolated directly from tumour tissue. First we established an isolation protocol for subpopulations of EVs from metastatic melanoma tissue, which included enzymatic treatment (collagenase D and DNase). Small and large EVs were isolated with differential ultracentrifugation, and these were further separated into high and low-density (HD and LD) fractions. All EV subpopulations were then analysed in depth using electron microscopy, Bioanalyzer\u00ae, nanoparticle tracking analysis, and quantitative mass spectrometry analysis. Subpopulations of EVs with distinct size, morphology, and RNA and protein cargo could be isolated from the metastatic melanoma tissue. LD EVs showed an RNA profile with the presence of 18S and 28S ribosomal subunits. In contrast, HD EVs had RNA profiles with small or no peaks for ribosomal RNA subunits. Quantitative proteomics showed that several proteins such as flotillin-1 were enriched in both large and small LD EVs, while ADAM10 were exclusively enriched in small LD EVs. In contrast, mitofilin was enriched only in the large EVs. We conclude that enzymatic treatments improve EV isolation from dense fibrotic tissue without any apparent effect on molecular or morphological characteristics. By providing a detailed categorization of several subpopulations of EVs isolated directly from tumour tissues, we might better understand the function of EVs in tumour biology and their possible use in biomarker discovery.", "doi": "10.1080/20013078.2020.1722433", "pmid": "32128073", "labels": {"Integrated Microscopy Technologies Gothenburg": "Service", "Glycoproteomics and MS Proteomics": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC7034452"}, {"db": "pii", "key": "1722433"}], "notes": [], "created": "2023-02-16T08:05:59.349Z", "modified": "2024-01-16T13:46:30.980Z"}, {"entity": "publication", "iuid": "45731fef896f4c55814ee2668776f539", "links": {"self": {"href": "https://publications.scilifelab.se/publication/45731fef896f4c55814ee2668776f539.json"}, "display": {"href": "https://publications.scilifelab.se/publication/45731fef896f4c55814ee2668776f539"}}, "title": "Subpopulations of extracellular vesicles from human metastatic melanoma tissue identified by quantitative proteomics after optimized isolation.", "authors": [{"family": "Crescitelli", "given": "Rossella", "initials": "R", "orcid": "0000-0002-1714-3169", "researcher": {"href": "https://publications.scilifelab.se/researcher/1d4b955f26ca4a679deb090e30d0ccf1.json"}}, {"family": "L\u00e4sser", "given": "Cecilia", "initials": "C", "orcid": "0000-0003-1279-1746", "researcher": {"href": "https://publications.scilifelab.se/researcher/e14e17d2cdb24a9f93991d83811c78b9.json"}}, {"family": "Jang", "given": "Su Chul", "initials": "SC", "orcid": "0000-0003-3326-1007", "researcher": {"href": "https://publications.scilifelab.se/researcher/d7866f9f05f74587af9a4d5beec0a1b5.json"}}, {"family": "Cvjetkovic", "given": "Aleksander", "initials": "A", "orcid": "0000-0002-9131-9791", "researcher": {"href": "https://publications.scilifelab.se/researcher/166451e5c8e54c0da297f6238c42c334.json"}}, {"family": "Malmh\u00e4ll", "given": "Carina", "initials": "C"}, {"family": "Karimi", "given": "Nasibeh", "initials": "N"}, {"family": "H\u00f6\u00f6g", "given": "Johanna L", "initials": "JL", "orcid": "0000-0003-2162-3816", "researcher": {"href": "https://publications.scilifelab.se/researcher/f1eaedff964f4060ae6e69f59cad4521.json"}}, {"family": "Johansson", "given": "Iva", "initials": "I", "orcid": "0000-0003-2162-3816", "researcher": {"href": "https://publications.scilifelab.se/researcher/f1eaedff964f4060ae6e69f59cad4521.json"}}, {"family": "Fuchs", "given": "Johannes", "initials": "J"}, {"family": "Thorsell", "given": "Annika", "initials": "A"}, {"family": "Gho", "given": "Yong Song", "initials": "YS"}, {"family": "Olofsson Bagge", "given": "R", "initials": "R", "orcid": "0000-0001-5795-0355", "researcher": {"href": "https://publications.scilifelab.se/researcher/e1f8016d5e64418ca71f230e64e415ba.json"}}, {"family": "L\u00f6tvall", "given": "Jan", "initials": "J", "orcid": "0000-0001-9195-9249", "researcher": {"href": "https://publications.scilifelab.se/researcher/0cbcaf6b5698411c92e0de9e8fcf390f.json"}}], "type": "journal article", "published": "2020-02-11", "journal": {"title": "J Extracell Vesicles", "issn": "2001-3078", "volume": "9", "issue": "1", "pages": "1722433", "issn-l": "2001-3078"}, "abstract": "The majority of extracellular vesicle (EV) studies conducted to date have been performed on cell lines with little knowledge on how well these represent the characteristics of EVs in vivo. The aim of this study was to establish a method to isolate and categorize subpopulations of EVs isolated directly from tumour tissue. First we established an isolation protocol for subpopulations of EVs from metastatic melanoma tissue, which included enzymatic treatment (collagenase D and DNase). Small and large EVs were isolated with differential ultracentrifugation, and these were further separated into high and low-density (HD and LD) fractions. All EV subpopulations were then analysed in depth using electron microscopy, Bioanalyzer\u00ae, nanoparticle tracking analysis, and quantitative mass spectrometry analysis. Subpopulations of EVs with distinct size, morphology, and RNA and protein cargo could be isolated from the metastatic melanoma tissue. LD EVs showed an RNA profile with the presence of 18S and 28S ribosomal subunits. In contrast, HD EVs had RNA profiles with small or no peaks for ribosomal RNA subunits. Quantitative proteomics showed that several proteins such as flotillin-1 were enriched in both large and small LD EVs, while ADAM10 were exclusively enriched in small LD EVs. In contrast, mitofilin was enriched only in the large EVs. We conclude that enzymatic treatments improve EV isolation from dense fibrotic tissue without any apparent effect on molecular or morphological characteristics. By providing a detailed categorization of several subpopulations of EVs isolated directly from tumour tissues, we might better understand the function of EVs in tumour biology and their possible use in biomarker discovery.", "doi": "10.1080/20013078.2020.1722433", "pmid": "32128073", "labels": {"Integrated Microscopy Technologies Gothenburg": "Service", "Glycoproteomics and MS Proteomics": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC7034452"}, {"db": "pii", "key": "1722433"}], "notes": [], "created": "2023-02-16T08:05:59.349Z", "modified": "2024-01-16T13:46:30.980Z"}, {"entity": "publication", "iuid": "e313c088a6984f53893826e3ca90fa07", "links": {"self": {"href": "https://publications.scilifelab.se/publication/e313c088a6984f53893826e3ca90fa07.json"}, "display": {"href": "https://publications.scilifelab.se/publication/e313c088a6984f53893826e3ca90fa07"}}, "title": "Axonemal doublet microtubules can split into two complete singlets in human sperm flagellum tips.", "authors": [{"family": "Zabeo", "given": "Davide", "initials": "D", "orcid": "0000-0002-5912-4601", "researcher": {"href": "https://publications.scilifelab.se/researcher/03943200c1ae493ab8b7d6886d0af356.json"}}, {"family": "Croft", "given": "Jacob T", "initials": "JT"}, {"family": "H\u00f6\u00f6g", "given": "Johanna L", "initials": "JL", "orcid": "0000-0003-2162-3816", "researcher": {"href": "https://publications.scilifelab.se/researcher/f1eaedff964f4060ae6e69f59cad4521.json"}}], "type": "journal article", "published": "2019-05-00", "journal": {"title": "FEBS Lett.", "issn": "1873-3468", "volume": "593", "issue": "9", "pages": "892-902", "issn-l": "0014-5793"}, "abstract": "Motile flagella are crucial for human fertility and embryonic development. The distal tip of the flagellum is where growth and intra-flagellar transport are coordinated. In most model organisms, but not all, the distal tip includes a 'singlet region', where axonemal doublet microtubules (dMT) terminate and only complete A-tubules extend as singlet microtubules (sMT) to the tip. How a human flagellar tip is structured is unknown. Here, the flagellar tip structure of human spermatozoa was investigated by cryo-electron tomography, revealing the formation of a complete sMT from both the A-tubule and B-tubule of dMTs. This different tip arrangement in human spermatozoa shows the need to investigate human flagella directly in order to understand their role in health and disease.", "doi": "10.1002/1873-3468.13379", "pmid": "30959570", "labels": {"Cryo-EM": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC6594080"}], "notes": [], "created": "2019-09-28T11:25:44.294Z", "modified": "2021-06-21T11:58:11.889Z"}]}