{"entity": "researcher", "timestamp": "2026-08-14T13:09:30.414Z", "family": "Ramstedt", "given": "Madeleine", "initials": "M", "orcid": "0000-0003-2646-8501", "affiliations": ["Department of Chemistry, UCMR Ume\u00e5 University  Ume\u00e5 Sweden"], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/f0fb139ad40341fd85e4ba6fe39eb7fe.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/f0fb139ad40341fd85e4ba6fe39eb7fe"}}, "publications": [{"entity": "publication", "iuid": "ed0d4a931b564baab6b46f325bf0351d", "links": {"self": {"href": "https://publications.scilifelab.se/publication/ed0d4a931b564baab6b46f325bf0351d.json"}, "display": {"href": "https://publications.scilifelab.se/publication/ed0d4a931b564baab6b46f325bf0351d"}}, "title": "Probing biofilm development, stress response and heterogeneity\u2014spectroscopic characterization of single and multi-species consortia", "authors": [{"family": "Yunda", "given": "Elena", "initials": "E"}, {"family": "Hagberg", "given": "Aleksandra", "initials": "A"}, {"family": "Duteil", "given": "Thibault", "initials": "T", "orcid": "0009-0008-3494-5949", "researcher": {"href": "https://publications.scilifelab.se/researcher/63a2e8cc983f46c69390628e8f1df8f9.json"}}, {"family": "Francius", "given": "Gr\u00e9gory", "initials": "G"}, {"family": "Gorzs\u00e1s", "given": "Andr\u00e1s", "initials": "A", "orcid": "0000-0002-2298-8844", "researcher": {"href": "https://publications.scilifelab.se/researcher/8070792ccead4c809c89943d84cf0e03.json"}}, {"family": "Quil\u00e8s", "given": "Fabienne", "initials": "F"}, {"family": "Ramstedt", "given": "Madeleine", "initials": "M", "orcid": "0000-0003-2646-8501", "researcher": {"href": "https://publications.scilifelab.se/researcher/f0fb139ad40341fd85e4ba6fe39eb7fe.json"}}], "type": "journal-article", "published": "2026-05-20", "journal": {"title": "NPJ Biofilms Microbiomes", "issn": "2055-5008", "volume": "12", "issue": "1", "issn-l": "2055-5008"}, "abstract": "Environmental bacterial biofilms play many roles in the ecosystem including cycling of nutrients and serving as food for grazing organisms. Their function is linked to their microbial and chemical composition that may be altered by several parameters including environmental stressors. This manuscript presents a well-characterized model system of four bacterial isolates from a small Swedish river: Pseudomonas sp., Sphingomonas sp., Rhizobium sp. and Pararhizobium sp. Microbiological and chemical phenotypes were investigated including cell and biofilm morphology, as well as biochemical composition in absence and presence of the drug trimethoprim. Vibrational spectroscopy, cryo-X-ray photoelectron spectroscopy and confocal optical microscopy were applied to investigate and characterize monocultures and cocultures. The chemical characterization showed variation of the energy storage substance polyhydroxyalkanoates as well as polysaccharides between isolates and drug exposures. Spatial heterogeneities were observed using Raman microspectroscopy where Sphingomonas sp. cells, formed small clusters, inside the four species consortium, an organization that appeared to protect this isolate during exposure to trimethoprim.", "doi": "10.1038/s41522-026-01010-x", "pmid": "42162010", "labels": {"Integrated Microscopy Technologies Ume\u00e5": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC13190702"}, {"db": "pii", "key": "10.1038/s41522-026-01010-x"}], "notes": [], "created": "2026-06-24T11:01:20.295Z", "modified": "2026-07-10T08:26:39.252Z"}, {"entity": "publication", "iuid": "0b5071faf9e94295890d2321eea1b81a", "links": {"self": {"href": "https://publications.scilifelab.se/publication/0b5071faf9e94295890d2321eea1b81a.json"}, "display": {"href": "https://publications.scilifelab.se/publication/0b5071faf9e94295890d2321eea1b81a"}}, "title": "Metabolic interactions between bacterial co-isolates from catheter-associated urinary tract infections", "authors": [{"family": "Sokol", "given": "Dmytro", "initials": "D"}, {"family": "Rzhepishevska", "given": "Olena", "initials": "O", "orcid": "0000-0002-7912-7447", "researcher": {"href": "https://publications.scilifelab.se/researcher/53216ecce7934eb3a4beedb82f318fc5.json"}}, {"family": "Marynova", "given": "Iryna", "initials": "I"}, {"family": "Monsen", "given": "Tor", "initials": "T"}, {"family": "Antti", "given": "Henrik", "initials": "H"}, {"family": "Ramstedt", "given": "Madeleine", "initials": "M", "orcid": "0000-0003-2646-8501", "researcher": {"href": "https://publications.scilifelab.se/researcher/f0fb139ad40341fd85e4ba6fe39eb7fe.json"}}], "type": "journal-article", "published": "2026-01-14", "journal": {"title": "Sci Rep", "issn": "2045-2322", "volume": "16", "issue": "1", "pages": "2061", "issn-l": "2045-2322"}, "abstract": "Catheter-associated urinary tract infections (CAUTI) are complex infections often involving multi-species bacteria. Escherichia coli is frequently an early coloniser. Subsequent colonisation by Pseudomonas aeruginosa and coexistence mechanisms between the two strains within urethral catheters is not yet fully understood. In this study, metabolic adaptations between co-isolated clinical E. coli and P. aeruginosa strains were investigated. It was found that P. aeruginosa outgrew E. coli in artificial urine medium (AUM), whereas E. coli dominated in culture broth such as Iso-sensitest. No evidence of direct antagonism was observed. Metabolite analyses revealed distinct metabolite patterns indicating cross-feeding and metabolic adaptations. In AUM, stress-response metabolites were elevated. Additionally, E. coli appeared to experience Fe-limitation in AUM, while the same was not observed for P. aeruginosa. The results highlight the influence of nutrient conditions on processes within mixed biofilms.\n\nThe online version contains supplementary material available at 10.1038/s41598-025-33855-1.", "doi": "10.1038/s41598-025-33855-1", "pmid": "41535363", "labels": {"Chemical Biology Consortium Sweden": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC12808099"}, {"db": "pii", "key": "10.1038/s41598-025-33855-1"}], "notes": [], "created": "2026-02-23T09:04:21.178Z", "modified": "2026-03-24T09:14:47.860Z"}, {"entity": "publication", "iuid": "beaa141e5dae4c429a581c0a53d7fd9b", "links": {"self": {"href": "https://publications.scilifelab.se/publication/beaa141e5dae4c429a581c0a53d7fd9b.json"}, "display": {"href": "https://publications.scilifelab.se/publication/beaa141e5dae4c429a581c0a53d7fd9b"}}, "title": "Quantitative and chemical adaptation of exopolymeric substances formed by a river microbial consortium during exposure to the antibiotic trimethoprim", "authors": [{"family": "Duteil", "given": "Thibault", "initials": "T", "orcid": "0009-0008-3494-5949", "researcher": {"href": "https://publications.scilifelab.se/researcher/63a2e8cc983f46c69390628e8f1df8f9.json"}}, {"family": "Gorzs\u00e1s", "given": "Andras", "initials": "A", "orcid": "0000-0002-2298-8844", "researcher": {"href": "https://publications.scilifelab.se/researcher/8070792ccead4c809c89943d84cf0e03.json"}}, {"family": "Ramstedt", "given": "Madeleine", "initials": "M", "orcid": "0000-0003-2646-8501", "researcher": {"href": "https://publications.scilifelab.se/researcher/f0fb139ad40341fd85e4ba6fe39eb7fe.json"}}], "type": "journal-article", "published": "2025-12-00", "journal": {"title": "Biofilm", "issn": "2590-2075", "volume": "10", "pages": "100334", "issn-l": null}, "abstract": null, "doi": "10.1016/j.bioflm.2025.100334", "pmid": null, "labels": {"Integrated Microscopy Technologies Ume\u00e5": "Service"}, "xrefs": [], "notes": [], "created": "2025-11-26T14:11:14.229Z", "modified": "2025-11-29T08:24:35.095Z"}, {"entity": "publication", "iuid": "3a5107c9b88641a49d9e4bba9137b4d1", "links": {"self": {"href": "https://publications.scilifelab.se/publication/3a5107c9b88641a49d9e4bba9137b4d1.json"}, "display": {"href": "https://publications.scilifelab.se/publication/3a5107c9b88641a49d9e4bba9137b4d1"}}, "title": "Antimicrobial peptide induced colloidal transformations in bacteria-mimetic vesicles: Combining in silico tools and experimental methods.", "authors": [{"family": "Freire", "given": "Rafael V M", "initials": "RVM"}, {"family": "Pillco-Valencia", "given": "Yeny", "initials": "Y"}, {"family": "da Hora", "given": "Gabriel C A", "initials": "GCA"}, {"family": "Ramstedt", "given": "Madeleine", "initials": "M", "orcid": "0000-0003-2646-8501", "researcher": {"href": "https://publications.scilifelab.se/researcher/f0fb139ad40341fd85e4ba6fe39eb7fe.json"}}, {"family": "Sandblad", "given": "Linda", "initials": "L", "orcid": "0000-0003-3492-3287", "researcher": {"href": "https://publications.scilifelab.se/researcher/070825e0190a4e9a932e79663d2bc89f.json"}}, {"family": "Soares", "given": "Thereza A", "initials": "TA"}, {"family": "Salentinig", "given": "Stefan", "initials": "S"}], "type": "journal article", "published": "2021-08-15", "journal": {"title": "Journal of Colloid and Interface Science", "issn": "1095-7103", "volume": "596", "pages": "352-363", "issn-l": "0021-9797"}, "abstract": "With the growing challenges of bacteria becoming resistant to conventional antibiotics, antimicrobial peptides (AMPs) may offer a potential alternative. One of the most studied AMPs, the human cathelicidin derived AMP LL-37 is notable for its antimicrobial activity even though its mechanism of action is not fully understood yet. This work investigates the interaction of LL-37 with 1-Palmitoyl-2-oleoyl-sn-glycero-3-phospho-rac-(1-glycerol) (POPG) vesicles, which were employed as a bacterial membrane model given the common presence of this phospholipid in the bacterial membrane. Experimental techniques including small angle X-ray scattering, transmission electron microscopy and dynamic light scattering were used to characterize the interactions among LL-37 and POPG. Molecular dynamics simulations complement the experimental studies with molecular-level insights into the process. LL-37 was discovered to actively and critically interact with the POPG vesicles, modifying the membrane curvature that eventually leads to structural transformations from vesicles to mixed micelles. The results shed light on the mechanisms underlying the interactions among LL-37 and bacteria mimetic vesicles and can guide the further development of AMP based antimicrobial materials and therapies.", "doi": "10.1016/j.jcis.2021.03.060", "pmid": "33839361", "labels": {"Cryo-EM": "Collaborative"}, "xrefs": [{"db": "pii", "key": "S0021-9797(21)00353-2"}], "notes": [], "created": "2021-12-13T22:01:44.654Z", "modified": "2023-12-04T10:17:50.022Z"}, {"entity": "publication", "iuid": "bba8d3b0e39d4c35b16e5b9a701a5718", "links": {"self": {"href": "https://publications.scilifelab.se/publication/bba8d3b0e39d4c35b16e5b9a701a5718.json"}, "display": {"href": "https://publications.scilifelab.se/publication/bba8d3b0e39d4c35b16e5b9a701a5718"}}, "title": "Surface analysis of bacterial systems using cryo\u2010X\u2010ray photoelectron spectroscopy", "authors": [{"family": "Hagberg", "given": "Aleksandra", "initials": "A"}, {"family": "Rzhepishevska", "given": "Olena", "initials": "O", "orcid": "0000-0002-7912-7447", "researcher": {"href": "https://publications.scilifelab.se/researcher/53216ecce7934eb3a4beedb82f318fc5.json"}}, {"family": "Semenets", "given": "Anastasiia", "initials": "A", "orcid": "0000-0002-6223-9506", "researcher": {"href": "https://publications.scilifelab.se/researcher/2dfc8da363494ad683ce39942f8f4303.json"}}, {"family": "Cisneros", "given": "David A", "initials": "DA", "orcid": "0000-0001-9919-0075", "researcher": {"href": "https://publications.scilifelab.se/researcher/19f7a4aba7af436899b5d63f95f0dcda.json"}}, {"family": "Ramstedt", "given": "Madeleine", "initials": "M", "orcid": "0000-0003-2646-8501", "researcher": {"href": "https://publications.scilifelab.se/researcher/f0fb139ad40341fd85e4ba6fe39eb7fe.json"}}], "type": "journal-article", "published": "2020-12-00", "journal": {"title": "Surf Interface Anal", "issn": "0142-2421", "issn-l": null, "volume": "52", "issue": "12", "pages": "792-801"}, "abstract": null, "doi": "10.1002/sia.6854", "pmid": null, "labels": {"Integrated Microscopy Technologies Ume\u00e5": "Service"}, "xrefs": [], "notes": [], "created": "2022-04-01T14:41:47.140Z", "modified": "2022-11-21T15:37:28.363Z"}]}