{"entity": "researcher", "timestamp": "2026-03-05T07:51:31.726Z", "family": "Rosales Rocabado", "given": "Juan Marcelo", "initials": "JM", "orcid": "0000-0002-8753-2979", "affiliations": ["Division of Bio-prosthodontics, Faculty of Dentistry & Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/eda8249af61547319e77b66721ea8191.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/eda8249af61547319e77b66721ea8191"}}, "publications": [{"entity": "publication", "iuid": "439b4f3e6f4a4cd087c14c33e9843759", "links": {"self": {"href": "https://publications.scilifelab.se/publication/439b4f3e6f4a4cd087c14c33e9843759.json"}, "display": {"href": "https://publications.scilifelab.se/publication/439b4f3e6f4a4cd087c14c33e9843759"}}, "title": "descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques.", "authors": [{"family": "Otomo", "given": "Kohei", "initials": "K", "orcid": "0000-0002-5322-6295", "researcher": {"href": "https://publications.scilifelab.se/researcher/533d35e986ee455586e089453d6a97d9.json"}}, {"family": "Omura", "given": "Takaki", "initials": "T"}, {"family": "Nozawa", "given": "Yuki", "initials": "Y", "orcid": "0009-0002-4571-6232", "researcher": {"href": "https://publications.scilifelab.se/researcher/fc582ce80ae24ab284c32facf997a994.json"}}, {"family": "Edwards", "given": "Steven J", "initials": "SJ"}, {"family": "Sato", "given": "Yukihiko", "initials": "Y"}, {"family": "Saito", "given": "Yuri", "initials": "Y"}, {"family": "Yagishita", "given": "Shigehiro", "initials": "S"}, {"family": "Uchida", "given": "Hitoshi", "initials": "H", "orcid": "0000-0002-8952-2061", "researcher": {"href": "https://publications.scilifelab.se/researcher/132470da90604fbb8d6f392e4302ffd5.json"}}, {"family": "Watakabe", "given": "Yuki", "initials": "Y", "orcid": "0000-0002-6580-1007", "researcher": {"href": "https://publications.scilifelab.se/researcher/6770fc27fcde42308b027f0517a38771.json"}}, {"family": "Naitou", "given": "Kiyotada", "initials": "K", "orcid": "0000-0001-8599-2528", "researcher": {"href": "https://publications.scilifelab.se/researcher/878767bc68214823b850dfb427575684.json"}}, {"family": "Yanai", "given": "Rin", "initials": "R"}, {"family": "Sahara", "given": "Naruhiko", "initials": "N"}, {"family": "Takagi", "given": "Satoshi", "initials": "S", "orcid": "0000-0001-9749-1034", "researcher": {"href": "https://publications.scilifelab.se/researcher/87d7e07cf7494536a2c70937d87f4515.json"}}, {"family": "Katayama", "given": "Ryohei", "initials": "R", "orcid": "0000-0001-7394-895X", "researcher": {"href": "https://publications.scilifelab.se/researcher/f933af571a2044ae9929841a8f53ae83.json"}}, {"family": "Iwata", "given": "Yusuke", "initials": "Y", "orcid": "0009-0009-7203-772X", "researcher": {"href": "https://publications.scilifelab.se/researcher/ebaed40358324e2ea2a9cbb9c3b0aef3.json"}}, {"family": "Shiokawa", "given": "Toshiro", "initials": "T"}, {"family": "Hayakawa", "given": "Yoku", "initials": "Y"}, {"family": "Otsuka", "given": "Kensuke", "initials": "K"}, {"family": "Watanabe-Takano", "given": "Haruko", "initials": "H", "orcid": "0000-0002-2986-3702", "researcher": {"href": "https://publications.scilifelab.se/researcher/47a79a191375419fb20e671f637c0a90.json"}}, {"family": "Haneda", "given": "Yuka", "initials": "Y", "orcid": "0000-0002-6178-1109", "researcher": {"href": "https://publications.scilifelab.se/researcher/f4accbf75ce2497988cf403c910f78fa.json"}}, {"family": "Fukuhara", "given": "Shigetomo", "initials": "S", "orcid": "0000-0003-2777-501X", "researcher": {"href": "https://publications.scilifelab.se/researcher/0819338144444c05bfa4d05fbc0a869f.json"}}, {"family": "Fujiwara", "given": "Miku", "initials": "M"}, {"family": "Nii", "given": "Takenobu", "initials": "T"}, {"family": "Meno", "given": "Chikara", "initials": "C", "orcid": "0000-0002-0869-6642", "researcher": {"href": "https://publications.scilifelab.se/researcher/104cf925fa24479f8d8561fdc6742496.json"}}, {"family": "Takeshita", "given": "Naoki", "initials": "N", "orcid": "0000-0002-1418-9188", "researcher": {"href": "https://publications.scilifelab.se/researcher/01c6c6da90e7462087eb38accca9d997.json"}}, {"family": "Yashiro", "given": "Kenta", "initials": "K"}, {"family": "Rosales Rocabado", "given": "Juan Marcelo", "initials": "JM", "orcid": "0000-0002-8753-2979", "researcher": {"href": "https://publications.scilifelab.se/researcher/eda8249af61547319e77b66721ea8191.json"}}, {"family": "Kaku", "given": "Masaru", "initials": "M", "orcid": "0000-0002-7988-6435", "researcher": {"href": "https://publications.scilifelab.se/researcher/9b49fea6ce3e43f3918a4ada8a55c6c4.json"}}, {"family": "Yamada", "given": "Tatsuya", "initials": "T", "orcid": "0000-0002-6906-9861", "researcher": {"href": "https://publications.scilifelab.se/researcher/727c7ecfeb4747f5aad5f8a106be8bce.json"}}, {"family": "Oishi", "given": "Yumiko", "initials": "Y"}, {"family": "Koike", "given": "Hiroyuki", "initials": "H", "orcid": "0000-0001-9934-6930", "researcher": {"href": "https://publications.scilifelab.se/researcher/99607b00ae124e13b29626412fde6f9d.json"}}, {"family": "Cheng", "given": "Yinglan", "initials": "Y"}, {"family": "Sekine", "given": "Keisuke", "initials": "K", "orcid": "0000-0001-5133-0876", "researcher": {"href": "https://publications.scilifelab.se/researcher/e87dc82e3059497d93d47fa4a7939e8a.json"}}, {"family": "Koga", "given": "Jun-Ichiro", "initials": "J"}, {"family": "Sugiyama", "given": "Kaori", "initials": "K"}, {"family": "Kimura", "given": "Kenichi", "initials": "K", "orcid": "0000-0002-0363-8865", "researcher": {"href": "https://publications.scilifelab.se/researcher/067d51f73a7745b7b61df312d75ea49d.json"}}, {"family": "Karube", "given": "Fuyuki", "initials": "F"}, {"family": "Kim", "given": "Hyeree", "initials": "H", "orcid": "0009-0007-3174-2989", "researcher": {"href": "https://publications.scilifelab.se/researcher/ec3bb7dfa8d243fcb85f0fa7a6a28a2e.json"}}, {"family": "Manabe", "given": "Ichiro", "initials": "I", "orcid": "0000-0001-9481-6673", "researcher": {"href": "https://publications.scilifelab.se/researcher/20749719d6974fd593ae69998f2efe0b.json"}}, {"family": "Nemoto", "given": "Tomomi", "initials": "T", "orcid": "0000-0001-6102-1495", "researcher": {"href": "https://publications.scilifelab.se/researcher/824c920cbacb4cc988fd3abf6aafa08b.json"}}, {"family": "Tainaka", "given": "Kazuki", "initials": "K", "orcid": "0000-0002-5943-8723", "researcher": {"href": "https://publications.scilifelab.se/researcher/a2ebee6e49614394880c6f5805174b46.json"}}, {"family": "Hamada", "given": "Akinobu", "initials": "A"}, {"family": "Brismar", "given": "Hjalmar", "initials": "H", "orcid": "0000-0003-0578-4003", "researcher": {"href": "https://publications.scilifelab.se/researcher/1ec23336e2ef4e298f340876f1136dce.json"}}, {"family": "Susaki", "given": "Etsuo A", "initials": "EA", "orcid": "0000-0001-5621-7311", "researcher": {"href": "https://publications.scilifelab.se/researcher/97fb49e8f6e843dab92b70890dbf9dc6.json"}}], "type": "journal article", "published": "2024-06-12", "journal": {"title": "Nat Commun", "issn": "2041-1723", "issn-l": "2041-1723", "volume": "15", "issue": "1", "pages": "4941"}, "abstract": "Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop-equipped SPIM for cleared specimens), a low-cost ($20,000-50,000), low-expertise (one-day installation by a non-expert), yet practical do-it-yourself light-sheet microscope as a solution for this bottleneck. Even the most fundamental configuration of descSPIM enables multi-color imaging of whole mouse brains and a cancer cell line-derived xenograft tumor mass for the visualization of neurocircuitry, assessment of drug distribution, and pathological examination by false-colored hematoxylin and eosin staining in a three-dimensional manner. Academically open-sourced ( https://github.com/dbsb-juntendo/descSPIM ), descSPIM allows routine three-dimensional imaging of cleared samples in minutes. Thus, the dissemination of descSPIM will accelerate biomedical discoveries driven by tissue clearing technologies.", "doi": "10.1038/s41467-024-49131-1", "pmid": "38866781", "labels": {"Integrated Microscopy Technologies Stockholm": "Technology development"}, "xrefs": [{"db": "pii", "key": "10.1038/s41467-024-49131-1"}], "notes": [], "created": "2024-06-14T08:32:48.936Z", "modified": "2024-11-25T17:48:31.705Z"}]}