{"entity": "researcher", "timestamp": "2026-07-17T18:15:50.404Z", "family": "Murina", "given": "Victoriia", "initials": "V", "orcid": "0000-0003-1523-1816", "affiliations": ["Department of Molecular Biology, Ume\u00e5 University, Ume\u00e5, Sweden.", "Laboratory for Molecular Infection Medicine Sweden (MIMS), Ume\u00e5 University, Ume\u00e5, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/eb97467a29cb494baa858f2a9c5287c8.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/eb97467a29cb494baa858f2a9c5287c8"}}, "publications": [{"entity": "publication", "iuid": "9db452ca3ba2471fadd10ad1333ec980", "links": {"self": {"href": "https://publications.scilifelab.se/publication/9db452ca3ba2471fadd10ad1333ec980.json"}, "display": {"href": "https://publications.scilifelab.se/publication/9db452ca3ba2471fadd10ad1333ec980"}}, "title": "Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogens.", "authors": [{"family": "Crowe-McAuliffe", "given": "Caillan", "initials": "C"}, {"family": "Murina", "given": "Victoriia", "initials": "V", "orcid": "0000-0003-1523-1816", "researcher": {"href": "https://publications.scilifelab.se/researcher/eb97467a29cb494baa858f2a9c5287c8.json"}}, {"family": "Turnbull", "given": "Kathryn Jane", "initials": "KJ"}, {"family": "Kasari", "given": "Marje", "initials": "M"}, {"family": "Mohamad", "given": "Merianne", "initials": "M"}, {"family": "Polte", "given": "Christine", "initials": "C"}, {"family": "Takada", "given": "Hiraku", "initials": "H", "orcid": "0000-0003-2034-5666", "researcher": {"href": "https://publications.scilifelab.se/researcher/5d9dfa63a3004266bc782f77263dcc9e.json"}}, {"family": "Vaitkevicius", "given": "Karolis", "initials": "K"}, {"family": "Johansson", "given": "J\u00f6rgen", "initials": "J"}, {"family": "Ignatova", "given": "Zoya", "initials": "Z"}, {"family": "Atkinson", "given": "Gemma C", "initials": "GC", "orcid": "0000-0002-4861-4584", "researcher": {"href": "https://publications.scilifelab.se/researcher/d810a2d001a74ccda5b376198896cbf6.json"}}, {"family": "O'Neill", "given": "Alex J", "initials": "AJ"}, {"family": "Hauryliuk", "given": "Vasili", "initials": "V", "orcid": "0000-0003-2389-5057", "researcher": {"href": "https://publications.scilifelab.se/researcher/17ea83b1d20f43f9872edbf89b275e5d.json"}}, {"family": "Wilson", "given": "Daniel N", "initials": "DN", "orcid": "0000-0003-3816-3828", "researcher": {"href": "https://publications.scilifelab.se/researcher/bd50ba35f7c74d7e9d4f42fbc49739f2.json"}}], "type": "journal article", "published": "2021-06-11", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "12", "issue": "1", "pages": "3577", "issn-l": "2041-1723"}, "abstract": "Target protection proteins confer resistance to the host organism by directly binding to the antibiotic target. One class of such proteins are the antibiotic resistance (ARE) ATP-binding cassette (ABC) proteins of the F-subtype (ARE-ABCFs), which are widely distributed throughout Gram-positive bacteria and bind the ribosome to alleviate translational inhibition from antibiotics that target the large ribosomal subunit. Here, we present single-particle cryo-EM structures of ARE-ABCF-ribosome complexes from three Gram-positive pathogens: Enterococcus faecalis LsaA, Staphylococcus haemolyticus VgaALC and Listeria monocytogenes VgaL. Supported by extensive mutagenesis analysis, these structures enable a general model for antibiotic resistance mediated by these ARE-ABCFs to be proposed. In this model, ABCF binding to the antibiotic-stalled ribosome mediates antibiotic release via mechanistically diverse long-range conformational relays that converge on a few conserved ribosomal RNA nucleotides located at the peptidyltransferase center. These insights are important for the future development of antibiotics that overcome such target protection resistance mechanisms.", "doi": "10.1038/s41467-021-23753-1", "pmid": "34117249", "labels": {"Cryo-EM": "Service"}, "xrefs": [{"db": "pii", "key": "10.1038/s41467-021-23753-1"}, {"db": "pmc", "key": "PMC8196190"}], "notes": [], "created": "2021-12-14T12:35:33.249Z", "modified": "2021-12-14T12:35:33.401Z"}]}