{"entity": "researcher", "timestamp": "2026-08-07T19:03:37.372Z", "family": "Martin", "given": "Frida", "initials": "F", "orcid": "0000-0002-3149-3835", "affiliations": ["Division of Parasitology, Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Box 7036, 750 07, Uppsala, Sweden. Frida.Martin@slu.se."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/eacd9c056fb1451994bf6ab3289bbeb1.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/eacd9c056fb1451994bf6ab3289bbeb1"}}, "publications": [{"entity": "publication", "iuid": "f04ae85417c5408ebe1f3ec31464321c", "links": {"self": {"href": "https://publications.scilifelab.se/publication/f04ae85417c5408ebe1f3ec31464321c.json"}, "display": {"href": "https://publications.scilifelab.se/publication/f04ae85417c5408ebe1f3ec31464321c"}}, "title": "Exploring the \u03b2-tubulin gene family in a benzimidazole-resistant Parascaris univalens population.", "authors": [{"family": "Martin", "given": "Frida", "initials": "F", "orcid": "0000-0002-3149-3835", "researcher": {"href": "https://publications.scilifelab.se/researcher/eacd9c056fb1451994bf6ab3289bbeb1.json"}}, {"family": "Halvarsson", "given": "Peter", "initials": "P"}, {"family": "Delhomme", "given": "Nicolas", "initials": "N"}, {"family": "H\u00f6glund", "given": "Johan", "initials": "J"}, {"family": "Tyd\u00e9n", "given": "Eva", "initials": "E"}], "type": "journal article", "published": "2021-08-26", "journal": {"title": "International Journal for Parasitology: Drugs and Drug Resistance", "issn": "2211-3207", "volume": "17", "pages": "84-91", "issn-l": "2211-3207"}, "abstract": "Benzimidazole (BZ) drugs are frequently used to treat infections with the equine ascarid Parascaris univalens due to increasing resistance to macrocyclic lactones and pyrantel. Benzimidazole resistance is rare in ascarids in contrast to strongyle parasites where this resistance is widespread. In strongyles, single nucleotide polymorphisms (SNPs) at codons 167, 198 and 200 in a \u03b2-tubulin gene have been correlated to BZ resistance, but little is known about the \u03b2-tubulin genes and their possible involvement in BZ resistance in P. univalens and other ascarids. Previously two \u03b2-tubulin genes have been identified in P. univalens. In this study, we present five additional \u03b2-tubulin genes as well as the phylogenetic relationship of all seven genes to \u03b2-tubulins of other clade III and V nematodes. In addition, the efficacy of fenbendazole for treatment of P. univalens on a Swedish stud farm was studied in 2019 and 2020 using faecal egg count reduction test. Reductions varied from 73% to 88%, indicating the presence of a resistant P. univalens population on the farm. The emergence of BZ resistance emphasizes the need for development of molecular markers for rapid and more sensitive detection of resistant populations. We therefore investigated whether possible SNPs at positions 167, 198 or 200 in any of the \u03b2-tubulin genes could be used to distinguish between resistant and susceptible P. univalens populations. Amplicon sequencing covering the mutation sites 167, 198 and 200 in all seven \u03b2-tubulin genes revealed an absence of SNPs in both resistant and susceptible populations, suggesting that the mechanism behind BZ resistance in ascarids is different from that in strongyle nematodes and the search for a molecular marker for BZ resistance in P. univalens needs to continue.", "doi": "10.1016/j.ijpddr.2021.08.004", "pmid": "34467878", "labels": {"National Genomics Infrastructure": "Service", "NGI Uppsala (Uppsala Genome Center)": "Service", "NGI Stockholm (Genomics Production)": "Service", "NGI Stockholm (Genomics Applications)": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pii", "key": "S2211-3207(21)00040-3"}, {"db": "pmc", "key": "PMC8406161"}], "notes": [], "created": "2021-09-22T15:36:40.621Z", "modified": "2024-01-16T13:48:38.626Z"}, {"entity": "publication", "iuid": "eb85d8ec7f9746d88a03ac6981ab0223", "links": {"self": {"href": "https://publications.scilifelab.se/publication/eb85d8ec7f9746d88a03ac6981ab0223.json"}, "display": {"href": "https://publications.scilifelab.se/publication/eb85d8ec7f9746d88a03ac6981ab0223"}}, "title": "Transcriptional responses in Parascaris univalens after in vitro exposure to ivermectin, pyrantel citrate and thiabendazole.", "authors": [{"family": "Martin", "given": "Frida", "initials": "F", "orcid": "0000-0002-3149-3835", "researcher": {"href": "https://publications.scilifelab.se/researcher/eacd9c056fb1451994bf6ab3289bbeb1.json"}}, {"family": "Dube", "given": "Faruk", "initials": "F"}, {"family": "Karlsson Lindsj\u00f6", "given": "Oskar", "initials": "O"}, {"family": "Eydal", "given": "Matth\u00edas", "initials": "M"}, {"family": "H\u00f6glund", "given": "Johan", "initials": "J"}, {"family": "Bergstr\u00f6m", "given": "Tomas F", "initials": "TF"}, {"family": "Tyd\u00e9n", "given": "Eva", "initials": "E"}], "type": "journal article", "published": "2020-07-09", "journal": {"title": "Parasit Vectors", "issn": "1756-3305", "volume": "13", "issue": "1", "pages": "342", "issn-l": "1756-3305"}, "abstract": "Parascaris univalens is a pathogenic parasite of foals and yearlings worldwide. In recent years, Parascaris spp. worms have developed resistance to several of the commonly used anthelmintics, though currently the mechanisms behind this development are unknown. The aim of this study was to investigate the transcriptional responses in adult P. univalens worms after in vitro exposure to different concentrations of three anthelmintic drugs, focusing on drug targets and drug metabolising pathways.\n\nAdult worms were collected from the intestines of two foals at slaughter. The foals were naturally infected and had never been treated with anthelmintics. Worms were incubated in cell culture media containing different concentrations of either ivermectin (10-9 M, 10-11 M, 10-13 M), pyrantel citrate (10-6 M, 10-8 M, 10-10 M), thiabendazole (10-5 M, 10-7 M, 10-9 M) or without anthelmintics (control) at 37 \u00b0C for 24 h. After incubation, the viability of the worms was assessed and RNA extracted from the anterior region of 36 worms and sequenced on an Illumina NovaSeq 6000 system.\n\nAll worms were alive at the end of the incubation but showed varying degrees of viability depending on the drug and concentration used. Differential expression (Padj < 0.05 and log2 fold change \u2265 1 or \u2264 - 1) analysis showed similarities and differences in the transcriptional response after exposure to the different drug classes. Candidate genes upregulated or downregulated in drug exposed worms include members of the phase I metabolic pathway short-chain dehydrogenase/reductase superfamily (SDR), flavin containing monooxygenase superfamily (FMO) and cytochrome P450-family (CYP), as well as members of the membrane transporters major facilitator superfamily (MFS) and solute carrier superfamily (SLC). Generally, different targets of the anthelmintics used were found to be upregulated and downregulated in an unspecific pattern after drug exposure, apart from the GABA receptor subunit lgc-37, which was upregulated only in worms exposed to 10-9 M of ivermectin.\n\nTo our knowledge, this is the first time the expression of lgc-37 and members of the FMO, SDR, MFS and SLC superfamilies have been described in P. univalens and future work should be focused on characterising these candidate genes to further explore their potential involvement in drug metabolism and anthelmintic resistance.", "doi": "10.1186/s13071-020-04212-0", "pmid": "32646465", "labels": {"National Genomics Infrastructure": "Service", "NGI Uppsala (SNP&SEQ Technology Platform)": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pii", "key": "10.1186/s13071-020-04212-0"}, {"db": "pmc", "key": "PMC7346371"}], "notes": [], "created": "2020-12-08T23:34:23.929Z", "modified": "2024-01-16T13:48:42.174Z"}]}