{"entity": "researcher", "timestamp": "2026-07-13T09:48:40.169Z", "family": "G\u00f6rgens", "given": "Andr\u00e9", "initials": "A", "orcid": "0000-0001-9198-0857", "affiliations": ["Department of Laboratory Medicine, Clinical Research Center, Karolinska Institutet, Stockholm, Sweden.", "Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/ea5f85e2c23a4515a39a2fa23d665a99.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/ea5f85e2c23a4515a39a2fa23d665a99"}}, "publications": [{"entity": "publication", "iuid": "b69e3a293cb74fbc9c8c46ecf0a78fbb", "links": {"self": {"href": "https://publications.scilifelab.se/publication/b69e3a293cb74fbc9c8c46ecf0a78fbb.json"}, "display": {"href": "https://publications.scilifelab.se/publication/b69e3a293cb74fbc9c8c46ecf0a78fbb"}}, "title": "Imaging Single Particle Profiler to Study Nanoscale Bioparticles Using Conventional Confocal Microscopy.", "authors": [{"family": "Sych", "given": "Taras", "initials": "T", "orcid": "0000-0002-7330-9063", "researcher": {"href": "https://publications.scilifelab.se/researcher/d104a7d8096b4a4ab2a9fe618489fc7e.json"}}, {"family": "G\u00f6rgens", "given": "Andr\u00e9", "initials": "A", "orcid": "0000-0001-9198-0857", "researcher": {"href": "https://publications.scilifelab.se/researcher/ea5f85e2c23a4515a39a2fa23d665a99.json"}}, {"family": "Steiner", "given": "Lo\u00efc", "initials": "L", "orcid": "0000-0001-6640-3513", "researcher": {"href": "https://publications.scilifelab.se/researcher/ef6ac5c6f9324434b11fc1cccd0a9027.json"}}, {"family": "Gucluler", "given": "Gozde", "initials": "G"}, {"family": "Huge", "given": "Ylva", "initials": "Y"}, {"family": "Alamdari", "given": "Farhood", "initials": "F"}, {"family": "Johansson", "given": "Markus", "initials": "M"}, {"family": "Aljabery", "given": "Firas", "initials": "F"}, {"family": "Sherif", "given": "Amir", "initials": "A", "orcid": "0000-0002-3675-3050", "researcher": {"href": "https://publications.scilifelab.se/researcher/e27ad5d3def147849d4821d86075c3d2.json"}}, {"family": "Gabrielsson", "given": "Susanne", "initials": "S", "orcid": "0000-0003-1771-1346", "researcher": {"href": "https://publications.scilifelab.se/researcher/1d446cb52d7e4a01ad8478a9cfe22ae1.json"}}, {"family": "El Andaloussi", "given": "Samir", "initials": "S", "orcid": "0000-0003-4468-9113", "researcher": {"href": "https://publications.scilifelab.se/researcher/bd1036a42043441da3e444f4eac58010.json"}}, {"family": "Sezgin", "given": "Erdinc", "initials": "E", "orcid": "0000-0002-4915-388X", "researcher": {"href": "https://publications.scilifelab.se/researcher/34d3b05d68d64f698ff08dc655d2fe26.json"}}], "type": "journal article", "published": "2025-02-12", "journal": {"title": "Nano Lett.", "issn": "1530-6992", "volume": "25", "issue": "6", "pages": "2173-2180", "issn-l": "1530-6984"}, "abstract": "Single particle profiling (SPP) is a unique methodology to study nanoscale bioparticles such as liposomes, lipid nanoparticles, extracellular vesicles, and lipoproteins in a single particle and high throughput manner. The initial version requires the single photon counting modules for data acquisition, which limits its adoptability. Here, we present imaging-based SPP (iSPP) that can be performed by imaging a spot over time in the common imaging mode with confocal detectors. We also provide user-friendly software with a graphical user interface to analyze such data and give quantitative insights on the content and properties of nanoscale bioparticles. We use iSPP to decipher lipid-protein interactions, membrane modifications by drugs, and the heterogeneity of extracellular vesicles isolated from cell lines and human urine. This easily applicable modality of the single particle profiler will facilitate nanoscale bioparticle research in laboratories with access to any confocal microscope.", "doi": "10.1021/acs.nanolett.4c05117", "pmid": "39878336", "labels": {"Integrated Microscopy Technologies Stockholm": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11827106"}], "notes": [], "created": "2025-03-11T12:50:21.409Z", "modified": "2025-03-24T08:21:15.353Z"}, {"entity": "publication", "iuid": "98c3436054da482d8ed7c2c09222f5c2", "links": {"self": {"href": "https://publications.scilifelab.se/publication/98c3436054da482d8ed7c2c09222f5c2.json"}, "display": {"href": "https://publications.scilifelab.se/publication/98c3436054da482d8ed7c2c09222f5c2"}}, "title": "Extracellular Histones as Exosome Membrane Proteins Regulated by Cell Stress.", "authors": [{"family": "Singh", "given": "Birendra", "initials": "B"}, {"family": "Fredriksson Sundbom", "given": "Marcus", "initials": "M", "orcid": "0000-0002-3586-4197", "researcher": {"href": "https://publications.scilifelab.se/researcher/b9fb80c88da9467c9984f169cd3da016.json"}}, {"family": "Muthukrishnan", "given": "Uma", "initials": "U"}, {"family": "Natarajan", "given": "Balasubramanian", "initials": "B"}, {"family": "Stransky", "given": "Stephanie", "initials": "S"}, {"family": "G\u00f6rgens", "given": "Andr\u00e9", "initials": "A", "orcid": "0000-0001-9198-0857", "researcher": {"href": "https://publications.scilifelab.se/researcher/ea5f85e2c23a4515a39a2fa23d665a99.json"}}, {"family": "Nordin", "given": "Joel Z", "initials": "JZ", "orcid": "0000-0002-3653-7710", "researcher": {"href": "https://publications.scilifelab.se/researcher/37075c4179534fc8a927c4e1330e92c7.json"}}, {"family": "Wiklander", "given": "Oscar P B", "initials": "OPB"}, {"family": "Sandblad", "given": "Linda", "initials": "L"}, {"family": "Sidoli", "given": "Simone", "initials": "S"}, {"family": "El Andaloussi", "given": "Samir", "initials": "S"}, {"family": "Haney", "given": "Michael", "initials": "M", "orcid": "0000-0002-4049-8910", "researcher": {"href": "https://publications.scilifelab.se/researcher/837c6b12b0ad44f8b1e2a26cb7cd245b.json"}}, {"family": "Gilthorpe", "given": "Jonathan D", "initials": "JD", "orcid": "0000-0002-6884-4774", "researcher": {"href": "https://publications.scilifelab.se/researcher/7eb2a4f38fdc4e0db2516a17d9abdf06.json"}}], "type": "journal article", "published": "2025-02-00", "journal": {"title": "J Extracell Vesicles", "issn": "2001-3078", "volume": "14", "issue": "2", "pages": "e70042", "issn-l": "2001-3078"}, "abstract": "Histones are conserved nuclear proteins that function as part of the nucleosome in the regulation of chromatin structure and gene expression. Interestingly, extracellular histones populate biofluids from healthy individuals, and when elevated, may contribute to various acute and chronic diseases. It is generally assumed that most extracellular histones exist as nucleosomes, as components of extracellular chromatin. We analysed cell culture models under normal and stressed conditions to identify pathways of histone secretion. We report that core and linker histones localize to extracellular vesicles (EVs) and are secreted via the multivesicular body/exosome pathway. Upregulation of EV histone secretion occurs in response to cellular stress, with enhanced vesicle secretion and a shift towards a population of smaller EVs. Most histones were membrane associated with the outer surface of EVs. Degradation of EV-DNA did not impact significantly on EV-histone association. Individual histones and histone octamers bound strongly to liposomes and EVs, but nucleosomes did not, showing histones do not require DNA for EV binding. Histones colocalized to tetraspanin positive EVs but using genetic or pharmacological intervention, we found that all known pathways of exosome biogenesis acted positively on histone secretion. Inhibition of autophagy and lysosomal degradation had a strong positive effect on EV histone release. Unexpectedly, EV-associated histones lacked the extensive post-translational modification of their nuclear counterparts, suggesting loss of PTMs may be involved in their trafficking or secretion. Our data does not support a significant role for EV-histones existing as nucleosomes. We show for the first time that histones are secreted from cells as membrane proteins via EVs/exosomes. This fundamental discovery provides support for further investigation of the biological activity of exosome associated histones and their role in disease.", "doi": "10.1002/jev2.70042", "pmid": "39976275", "labels": {"Cryo-EM": "Collaborative", "Integrated Microscopy Technologies Ume\u00e5": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC11840699"}], "notes": [], "created": "2025-11-03T12:14:46.643Z", "modified": "2025-11-18T13:15:12.096Z"}, {"entity": "publication", "iuid": "b5090ee7f17e4311876f2e97125ba47d", "links": {"self": {"href": "https://publications.scilifelab.se/publication/b5090ee7f17e4311876f2e97125ba47d.json"}, "display": {"href": "https://publications.scilifelab.se/publication/b5090ee7f17e4311876f2e97125ba47d"}}, "title": "Cutaneous squamous cell carcinoma-derived extracellular vesicles exert an oncogenic role by activating cancer-associated fibroblasts.", "authors": [{"family": "Li", "given": "Chen", "initials": "C", "orcid": "0000-0002-7199-4298", "researcher": {"href": "https://publications.scilifelab.se/researcher/acadd10dfac94eda9e9c951a8c04d6cb.json"}}, {"family": "Sun", "given": "Chengxi", "initials": "C", "orcid": "0000-0002-1050-5956", "researcher": {"href": "https://publications.scilifelab.se/researcher/8b42a5450dfd4211981fee0603acf321.json"}}, {"family": "Lohcharoenkal", "given": "Warangkana", "initials": "W"}, {"family": "Ali", "given": "Mohamad Moustafa", "initials": "MM", "orcid": "0000-0002-4902-0550", "researcher": {"href": "https://publications.scilifelab.se/researcher/780c944670ff4d7489410895569ac257.json"}}, {"family": "Xing", "given": "Pengwei", "initials": "P"}, {"family": "Zheng", "given": "Wenyi", "initials": "W"}, {"family": "G\u00f6rgens", "given": "Andr\u00e9", "initials": "A", "orcid": "0000-0001-9198-0857", "researcher": {"href": "https://publications.scilifelab.se/researcher/ea5f85e2c23a4515a39a2fa23d665a99.json"}}, {"family": "Gustafsson", "given": "Manuela O", "initials": "MO"}, {"family": "El Andaloussi", "given": "Samir", "initials": "S"}, {"family": "Sonkoly", "given": "Enik\u00f6", "initials": "E"}, {"family": "Pivarcsi", "given": "Andor", "initials": "A", "orcid": "0000-0003-2196-1102", "researcher": {"href": "https://publications.scilifelab.se/researcher/77ca870317234573a3da5dffb24bb268.json"}}], "type": "journal article", "published": "2023-07-26", "journal": {"title": "Cell Death Discov", "issn": "2058-7716", "volume": "9", "issue": "1", "pages": "260", "issn-l": "2058-7716"}, "abstract": "Cutaneous squamous cell carcinoma (cSCC) is a fast-increasing cancer with metastatic potential. Extracellular vesicles (EVs) are small membrane-bound vesicles that play important roles in intercellular communication, particularly in the tumor microenvironment (TME). Here we report that cSCC cells secrete an increased number of EVs relative to normal human epidermal keratinocytes (NHEKs) and that interfering with the capacity of cSCC to secrete EVs inhibits tumor growth in vivo in a xenograft model of human cSCC. Transcriptome analysis of tumor xenografts by RNA-sequencing enabling the simultaneous quantification of both the human and the mouse transcripts revealed that impaired EV-production of cSCC cells prominently altered the phenotype of stromal cells, in particular genes related to extracellular matrix (ECM)-formation and epithelial-mesenchymal transition (EMT). In line with these results, co-culturing of human dermal fibroblasts (HDFs) with cSCC cells, but not with normal keratinocytes in vitro resulted in acquisition of cancer-associated fibroblast (CAF) phenotype. Interestingly, EVs derived from metastatic cSCC cells, but not primary cSCCs or NHEKs, were efficient in converting HDFs to CAFs. Multiplex bead-based flow cytometry assay and mass-spectrometry (MS)-based proteomic analyses revealed the heterogenous cargo of cSCC-derived EVs and that especially EVs derived from metastatic cSCCs carry proteins associated with EV-biogenesis, EMT, and cell migration. Mechanistically, EVs from metastatic cSCC cells result in the activation of TGF\u03b2 signaling in HDFs. Altogether, our study suggests that cSCC-derived EVs mediate cancer-stroma communication, in particular the conversion of fibroblasts to CAFs, which eventually contribute to cSCC progression.", "doi": "10.1038/s41420-023-01555-2", "pmid": "37495566", "labels": {"NGI Short read": "Service", "NGI Uppsala (SNP&SEQ Technology Platform)": "Service", "National Genomics Infrastructure": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC10372068"}, {"db": "pii", "key": "10.1038/s41420-023-01555-2"}], "notes": [], "created": "2023-11-29T11:23:30.604Z", "modified": "2024-01-16T13:48:32.858Z"}]}