{"entity": "researcher", "timestamp": "2026-07-14T04:24:57.217Z", "family": "\u00d6st", "given": "Anita", "initials": "A", "orcid": "0000-0003-0547-1904", "affiliations": ["Department of Biomedical and Clinical Sciences, Link\u00f6ping University, Link\u00f6ping, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/e7a222183b264e4b828a38c322a4090f.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/e7a222183b264e4b828a38c322a4090f"}}, "publications": [{"entity": "publication", "iuid": "ea0141d3c2e3447facf702d344e4d0f3", "links": {"self": {"href": "https://publications.scilifelab.se/publication/ea0141d3c2e3447facf702d344e4d0f3.json"}, "display": {"href": "https://publications.scilifelab.se/publication/ea0141d3c2e3447facf702d344e4d0f3"}}, "title": "Small RNA in sperm-Paternal contributions to human embryo development.", "authors": [{"family": "Isacson", "given": "Signe", "initials": "S", "orcid": "0000-0002-7590-8326", "researcher": {"href": "https://publications.scilifelab.se/researcher/c0cb5b0f3f694a10abf049f9e9c298e3.json"}}, {"family": "Karlsson", "given": "Kajsa", "initials": "K", "orcid": "0009-0002-7481-6291", "researcher": {"href": "https://publications.scilifelab.se/researcher/5f9727c26319451fab05d0646447c9c3.json"}}, {"family": "Zalavary", "given": "Stefan", "initials": "S", "orcid": "0000-0001-5209-6655", "researcher": {"href": "https://publications.scilifelab.se/researcher/5ccbf5fd93a3471d828865b7da39571e.json"}}, {"family": "Asratian", "given": "Anna", "initials": "A", "orcid": "0000-0001-7696-0508", "researcher": {"href": "https://publications.scilifelab.se/researcher/420e14758e1f45e9bf5f8a37f0115eae.json"}}, {"family": "Kugelberg", "given": "Unn", "initials": "U"}, {"family": "Liffner", "given": "Susanne", "initials": "S"}, {"family": "\u00d6st", "given": "Anita", "initials": "A", "orcid": "0000-0003-0547-1904", "researcher": {"href": "https://publications.scilifelab.se/researcher/e7a222183b264e4b828a38c322a4090f.json"}}], "type": "journal article", "published": "2025-07-17", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "16", "issue": "1", "pages": "6571", "issn-l": "2041-1723"}, "abstract": "Sperm not only delivers the paternal genome to the oocyte but also regulatory small RNA (sRNA). However, the role of sRNA in fertilisation and human embryo development remains poorly understood. Here, couples undergoing IVF are recruited, and sperm sRNA analysed to investigate their role in IVF treatment. Differential expression of mitochondrial sRNA and Y-RNA are observed in relation to sperm concentration. For fertilisation rate, sRNA from a single locus are significantly changed. Expression of microRNA (miRNA) and ribosomal sRNA correlates positively and negatively, respectively, to high-quality embryos. Notably, the top miRNA have an area under ROC of >0.8. Predicted targets of these miRNA are relevant for development, suggesting a role for sperm-borne miRNA in embryo development. In conclusion, sperm-borne sRNA are biomarkers for sperm concentration and embryo quality in IVF. These findings may contribute to clinical strategies improving embryo quality, lowering costs and reducing the need for additional treatment cycles.", "doi": "10.1038/s41467-025-62015-2", "pmid": "40670377", "labels": {"Clinical Genomics Link\u00f6ping": "Service", "Clinical Genomics": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC12267487"}, {"db": "pii", "key": "10.1038/s41467-025-62015-2"}], "notes": [], "created": "2025-08-11T10:09:40.254Z", "modified": "2025-08-11T10:09:40.970Z"}, {"entity": "publication", "iuid": "f662260585b8490d92cb95f40eb405ca", "links": {"self": {"href": "https://publications.scilifelab.se/publication/f662260585b8490d92cb95f40eb405ca.json"}, "display": {"href": "https://publications.scilifelab.se/publication/f662260585b8490d92cb95f40eb405ca"}}, "title": "Stress-sensitive dynamics of miRNAs and Elba1 in Drosophila embryogenesis.", "authors": [{"family": "\u00d6rkenby", "given": "Lovisa", "initials": "L", "orcid": "0000-0002-6015-7240", "researcher": {"href": "https://publications.scilifelab.se/researcher/c0a1d51d61264021818dd484cc6ddaff.json"}}, {"family": "Skog", "given": "Signe", "initials": "S", "orcid": "0000-0002-7590-8326", "researcher": {"href": "https://publications.scilifelab.se/researcher/c0cb5b0f3f694a10abf049f9e9c298e3.json"}}, {"family": "Ekman", "given": "Helen", "initials": "H", "orcid": "0000-0002-0181-6170", "researcher": {"href": "https://publications.scilifelab.se/researcher/7dff0e6e3025446084e6fc3e0254abe7.json"}}, {"family": "Gozzo", "given": "Alessandro", "initials": "A", "orcid": "0000-0002-6466-627X", "researcher": {"href": "https://publications.scilifelab.se/researcher/37245c697b3c4e119f91a9f44f47607a.json"}}, {"family": "Kugelberg", "given": "Unn", "initials": "U"}, {"family": "Ramesh", "given": "Rashmi", "initials": "R", "orcid": "0000-0003-3685-7893", "researcher": {"href": "https://publications.scilifelab.se/researcher/08615ef9bb444dea8b40973374177ae6.json"}}, {"family": "Magadi", "given": "Srivathsa", "initials": "S", "orcid": "0000-0002-7926-5970", "researcher": {"href": "https://publications.scilifelab.se/researcher/61353f2ecb17431594018661b201f8da.json"}}, {"family": "Zambanini", "given": "Gianluca", "initials": "G", "orcid": "0000-0003-1275-6027", "researcher": {"href": "https://publications.scilifelab.se/researcher/37637bc1358a4928b87eff29af644efe.json"}}, {"family": "Nordin", "given": "Anna", "initials": "A", "orcid": "0000-0002-5868-4797", "researcher": {"href": "https://publications.scilifelab.se/researcher/f2e576298b274e1b8eba78f3f6df759f.json"}}, {"family": "Cant\u00fa", "given": "Claudio", "initials": "C", "orcid": "0000-0003-1547-5415", "researcher": {"href": "https://publications.scilifelab.se/researcher/c005c54c7c5845c39c413cd2d8062aaa.json"}}, {"family": "N\u00e4tt", "given": "Daniel", "initials": "D", "orcid": "0000-0001-9182-9401", "researcher": {"href": "https://publications.scilifelab.se/researcher/6af95d8800274851babd3c3ecae29c55.json"}}, {"family": "\u00d6st", "given": "Anita", "initials": "A", "orcid": "0000-0003-0547-1904", "researcher": {"href": "https://publications.scilifelab.se/researcher/e7a222183b264e4b828a38c322a4090f.json"}}], "type": "journal article", "published": "2023-05-09", "journal": {"title": "Mol. Syst. Biol.", "issn": "1744-4292", "volume": "19", "issue": "5", "pages": "e11148", "issn-l": "1744-4292"}, "abstract": "Early-life stress can result in life-long effects that impact adult health and disease risk, but little is known about how such programming is established and maintained. Here, we show that such epigenetic memories can be initiated in the Drosophila embryo before the major wave of zygotic transcription, and higher-order chromatin structures are established. An early short heat shock results in elevated levels of maternal miRNA and reduced levels of a subgroup of zygotic genes in stage 5 embryos. Using a Dicer-1 mutant, we show that the stress-induced decrease in one of these genes, the insulator-binding factor Elba1, is dependent on functional miRNA biogenesis. Reduction in Elba1 correlates with the upregulation of early developmental genes and promotes a sustained weakening of heterochromatin in the adult fly as indicated by an increased expression of the PEV wm4h reporter. We propose that maternal miRNAs, retained in response to an early embryonic heat shock, shape the subsequent de novo heterochromatin establishment that occurs during early development via direct or indirect regulation of some of the earliest expressed genes, including Elba1.", "doi": "10.15252/msb.202211148", "pmid": "36938679", "labels": {"Clinical Genomics Link\u00f6ping": "Service", "Clinical Genomics": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC10167479"}], "notes": [], "created": "2023-12-03T05:45:48.775Z", "modified": "2023-12-03T05:45:49.133Z"}, {"entity": "publication", "iuid": "b5a78af9634b44bd8bb3760184a70aab", "links": {"self": {"href": "https://publications.scilifelab.se/publication/b5a78af9634b44bd8bb3760184a70aab.json"}, "display": {"href": "https://publications.scilifelab.se/publication/b5a78af9634b44bd8bb3760184a70aab"}}, "title": "5\u00b4XP sRNA-seq: efficient identification of transcripts with and without 5\u00b4 phosphorylation reveals evolutionary conserved small RNA.", "authors": [{"family": "Kugelberg", "given": "Unn", "initials": "U"}, {"family": "N\u00e4tt", "given": "Daniel", "initials": "D", "orcid": "0000-0001-9182-9401", "researcher": {"href": "https://publications.scilifelab.se/researcher/6af95d8800274851babd3c3ecae29c55.json"}}, {"family": "Skog", "given": "Signe", "initials": "S"}, {"family": "Kutter", "given": "Claudia", "initials": "C"}, {"family": "\u00d6st", "given": "Anita", "initials": "A", "orcid": "0000-0003-0547-1904", "researcher": {"href": "https://publications.scilifelab.se/researcher/e7a222183b264e4b828a38c322a4090f.json"}}], "type": "journal article", "published": "2021-11-00", "journal": {"title": "RNA Biol", "issn": "1555-8584", "issn-l": "1547-6286", "volume": "18", "issue": "11", "pages": "1588-1599"}, "abstract": "Small RNA (sRNA) sequencing has been critical for our understanding of many cellular processes, including gene regulation. Nonetheless, the varying biochemical properties of sRNA, such as 5\u00b4 nucleotide modifications, make many sRNA subspecies incompatible with common protocols for sRNA sequencing. Here we describe 5XP-seq that outlines a novel strategy that captures a more complete picture of sRNA. By tagging 5\u00b4P sRNA during library preparation, 5XP-seq combines an open approach that includes all types of 5'-terminal modifications (5\u00b4X), with a selective approach for 5-phosphorylated sRNA (5\u00b4P). We show that 5XP-seq not only enriches phosphorylated miRNA and piRNA but successfully discriminates these sRNA from all other sRNA species. We further demonstrate the importance of this strategy by successful inter-species validation of sRNAs that would have otherwise failed, including human to insect translation of several tRNA (tRFs) and rRNA (rRFs) fragments. By combining 5\u00b4 insensitive library strategies with 5\u00b4 sensitive tagging, we have successfully tackled an intrinsic bias in modern sRNA sequencing that will help us reveal the true complexity and the evolutionary significance of the sRNA world.", "doi": "10.1080/15476286.2020.1861770", "pmid": "33382953", "labels": {"Clinical Genomics Link\u00f6ping": "Service", "Clinical Genomics": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC8594926"}], "notes": [], "created": "2021-12-09T13:05:58.642Z", "modified": "2021-12-09T13:06:07.959Z"}]}