{"entity": "researcher", "timestamp": "2026-07-20T22:54:12.805Z", "family": "Rosendal", "given": "Ebba", "initials": "E", "orcid": "0000-0001-8512-0535", "affiliations": ["Department of Clinical Microbiology, Ume\u00e5 University, Ume\u00e5, Sweden.", "The Laboratory for Molecular Infection Medicine Sweden (MIMS), Ume\u00e5, Sweden.", "Ume\u00e5 Center for Microbial Research, Ume\u00e5, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/dda3f5ae552241eea145e011afdfad20.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/dda3f5ae552241eea145e011afdfad20"}}, "publications": [{"entity": "publication", "iuid": "b017a77ea50343cb82a59c299292a958", "links": {"self": {"href": "https://publications.scilifelab.se/publication/b017a77ea50343cb82a59c299292a958.json"}, "display": {"href": "https://publications.scilifelab.se/publication/b017a77ea50343cb82a59c299292a958"}}, "title": "Cryo-electron tomography reveals coupled flavivirus replication, budding and maturation.", "authors": [{"family": "Dahmane", "given": "Selma", "initials": "S", "orcid": "0000-0003-4193-1496", "researcher": {"href": "https://publications.scilifelab.se/researcher/78124ba9dbac444aaee032c91c235a88.json"}}, {"family": "Schexnaydre", "given": "Erin", "initials": "E", "orcid": "0000-0002-2993-8647", "researcher": {"href": "https://publications.scilifelab.se/researcher/f643b7350ed5404aac418ce43d907f27.json"}}, {"family": "Zhang", "given": "Jianguo", "initials": "J"}, {"family": "Singh", "given": "Bina K", "initials": "BK"}, {"family": "Rosendal", "given": "Ebba", "initials": "E", "orcid": "0000-0001-8512-0535", "researcher": {"href": "https://publications.scilifelab.se/researcher/dda3f5ae552241eea145e011afdfad20.json"}}, {"family": "Chotiwan", "given": "Nunya", "initials": "N", "orcid": "0000-0003-3214-6605", "researcher": {"href": "https://publications.scilifelab.se/researcher/aba9da05817a4a8ea7037131000d6a7f.json"}}, {"family": "Sharma", "given": "Kiran B", "initials": "KB"}, {"family": "Nilsson", "given": "Emma", "initials": "E", "orcid": "0000-0001-8123-3292", "researcher": {"href": "https://publications.scilifelab.se/researcher/6070627ce8644379afe4a239a23c0f93.json"}}, {"family": "Peters", "given": "Marie B A", "initials": "MBA", "orcid": "0000-0001-8994-0864", "researcher": {"href": "https://publications.scilifelab.se/researcher/df4733590d054742b732c2028a8f5e8a.json"}}, {"family": "Yau", "given": "Wai-Lok", "initials": "WL"}, {"family": "R\u00f6nfeldt", "given": "Sebastian", "initials": "S"}, {"family": "Lundmark", "given": "Richard", "initials": "R", "orcid": "0000-0001-9104-724X", "researcher": {"href": "https://publications.scilifelab.se/researcher/3e1b756caa79468dab0f960e43cd61d3.json"}}, {"family": "Barad", "given": "Benjamin A", "initials": "BA", "orcid": "0000-0002-1016-862X", "researcher": {"href": "https://publications.scilifelab.se/researcher/8a118a58f2f140bab794786ac581d3ac.json"}}, {"family": "Grotjahn", "given": "Danielle A", "initials": "DA", "orcid": "0000-0001-5908-7882", "researcher": {"href": "https://publications.scilifelab.se/researcher/e9c2292eb2294f56bcc068a7a01737a8.json"}}, {"family": "Liese", "given": "Susanne", "initials": "S"}, {"family": "Carlson", "given": "Andreas", "initials": "A", "orcid": "0000-0002-3068-9983", "researcher": {"href": "https://publications.scilifelab.se/researcher/39f4cc3bec4747da984c7c382a8ca5ae.json"}}, {"family": "\u00d6verby", "given": "Anna K", "initials": "AK", "orcid": "0000-0001-6553-0940", "researcher": {"href": "https://publications.scilifelab.se/researcher/506b0e2b2d884f868df73c7663b9ffb7.json"}}, {"family": "Carlson", "given": "Lars-Anders", "initials": "LA", "orcid": "0000-0003-2342-6488", "researcher": {"href": "https://publications.scilifelab.se/researcher/ba0e366ce21e49b48212bbed5a0a7bd1.json"}}], "type": "journal article", "published": "2026-01-20", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "17", "issue": "1", "pages": "828", "issn-l": "2041-1723"}, "abstract": "Flaviviruses replicate their genomes in replication organelles (ROs) formed as bud-like invaginations on the endoplasmic reticulum membrane, which also functions as the site for virion assembly. While this localization is well established, it is not known to what extent viral membrane remodeling, genome replication, virion assembly, and maturation are coordinated. Here, we image tick-borne flavivirus replication in human cells using cryo-electron tomography. We find that the RO membrane bud is shaped by a combination of a curvature-establishing membrane modification and the pressure from intraluminal template RNA. A protein complex at the RO base extends to an adjacent membrane, where immature virus particles bud. Naturally occurring furin site variants determine whether virus particles mature in the immediate vicinity of ROs. We further visualize replication in mouse brain tissue by cryo-electron tomography. Taken together, these findings reveal a close spatial coupling of flavivirus genome replication, budding, and maturation.", "doi": "10.1038/s41467-026-68483-4", "pmid": "41559045", "labels": {"Cryo-EM": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC12824359"}, {"db": "pii", "key": "10.1038/s41467-026-68483-4"}], "notes": [], "created": "2026-06-24T10:27:41.675Z", "modified": "2026-06-24T10:28:01.603Z"}, {"entity": "publication", "iuid": "467584c1e0774aef801f14804cc86726", "links": {"self": {"href": "https://publications.scilifelab.se/publication/467584c1e0774aef801f14804cc86726.json"}, "display": {"href": "https://publications.scilifelab.se/publication/467584c1e0774aef801f14804cc86726"}}, "title": "Influence of the pre-membrane and envelope proteins on structure, pathogenicity, and tropism of tick-borne encephalitis virus.", "authors": [{"family": "Rosendal", "given": "Ebba", "initials": "E", "orcid": "0000-0001-8512-0535", "researcher": {"href": "https://publications.scilifelab.se/researcher/dda3f5ae552241eea145e011afdfad20.json"}}, {"family": "Bisikalo", "given": "Kyrylo", "initials": "K"}, {"family": "Willekens", "given": "Stefanie M A", "initials": "SMA"}, {"family": "Lindgren", "given": "Marie", "initials": "M"}, {"family": "Holoubek", "given": "Ji\u0159\u00ed", "initials": "J"}, {"family": "Svoboda", "given": "Pavel", "initials": "P"}, {"family": "Lappalainen", "given": "Amanda", "initials": "A"}, {"family": "K\u00f6nighofer", "given": "Ebba", "initials": "E"}, {"family": "Mirgorodskaya", "given": "Ekaterina", "initials": "E"}, {"family": "Nord\u00e9n", "given": "Rickard", "initials": "R"}, {"family": "Morini", "given": "Federico", "initials": "F"}, {"family": "Rosenbaum", "given": "William", "initials": "W"}, {"family": "R\u016f\u017eek", "given": "Daniel", "initials": "D"}, {"family": "Ahlgren", "given": "Ulf", "initials": "U"}, {"family": "Anastasina", "given": "Maria", "initials": "M"}, {"family": "Merits", "given": "Andres", "initials": "A", "orcid": "0000-0001-8193-0071", "researcher": {"href": "https://publications.scilifelab.se/researcher/f77e550339464de88e94f9aa9a91e324.json"}}, {"family": "Butcher", "given": "Sarah J", "initials": "SJ", "orcid": "0000-0001-7060-5871", "researcher": {"href": "https://publications.scilifelab.se/researcher/f7076e70172b45d7ab9de4ab86775ae9.json"}}, {"family": "Nilsson", "given": "Emma", "initials": "E"}, {"family": "\u00d6verby", "given": "Anna K", "initials": "AK", "orcid": "0000-0001-6553-0940", "researcher": {"href": "https://publications.scilifelab.se/researcher/506b0e2b2d884f868df73c7663b9ffb7.json"}}], "type": "journal article", "published": "2025-09-23", "journal": {"title": "J. Virol.", "issn": "1098-5514", "volume": "99", "issue": "9", "pages": "e0087025", "issn-l": "0022-538X"}, "abstract": "Tick-borne encephalitis virus (TBEV) is a neurotropic flavivirus that causes thousands of human infections annually. Viral tropism in the brain is determined by the presence of necessary receptors, entry factors, and the ability of the virus to overcome host defenses. The viral structural proteins, pre-membrane (prM), and envelope (E) play an important role in receptor binding, membrane fusion, particle maturation, and antibody neutralization. To understand how these proteins influence virus distribution and tropism in the brain, we generated a chimeric virus harboring the prM and ectodomain of E from TBEV in the background of the low-pathogenic Langat virus (LGTV). We solved the atomic structures of both the chimeric virus and LGTV to compare them to the known TBEV structure. We show that this chimeric virus remains low-pathogenic, while being structurally and antigenically similar to TBEV. Using 3D optical whole brain imaging combined with immunohistochemistry, we found that both LGTV and the chimeric virus primarily infect the cerebral cortex, with no significant differences in their localization or tropism. In contrast, TBEV shows high infection of the cerebellum and a strong preference toward Purkinje cells, indicating that factors other than the prM and E proteins are important for determining TBEV tropism in the brain. Together, this provides new insights into the roles of the structural and non-structural proteins of tick-borne flaviviruses.\n\nAlthough an effective vaccine exists, there is no treatment for those infected by the tick-borne encephalitis virus (TBEV). This study aimed to better understand how the virus's surface proteins influence viral tropism and pathogenicity. We created a chimeric virus with prM and E proteins of TBEV in the genetic background of the low-pathogenic Langat virus (LGTV). The chimeric virus remained low pathogenic, similar to LGTV. Both viruses infected similar brain regions, while TBEV showed a strong preference for the cerebellum and Purkinje cells. This means that other parts of the virus, such as non-structural proteins or NCR, likely decide how the virus behaves in the brain. This study also presents the first cryogenic electron microscopy structure of LGTV, the first whole-brain imaging of TBEV infection in mouse brain, and a new model system to study surface proteins in tick-borne flaviviruses-laying groundwork for future studies on viral tropism, antibody cross-reactivity, and virus-receptor interaction.", "doi": "10.1128/jvi.00870-25", "pmid": "40827915", "labels": {"Glycoproteomics and MS Proteomics": "Collaborative", "Cryo-EM": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC12456022"}], "notes": [], "created": "2025-10-22T19:22:15.679Z", "modified": "2025-11-13T09:31:25.575Z"}, {"entity": "publication", "iuid": "3365d1fc9ac7482a996bbeb10f42de8e", "links": {"self": {"href": "https://publications.scilifelab.se/publication/3365d1fc9ac7482a996bbeb10f42de8e.json"}, "display": {"href": "https://publications.scilifelab.se/publication/3365d1fc9ac7482a996bbeb10f42de8e"}}, "title": "Cryo-electron tomography reveals coupled flavivirus replication, budding and maturation.", "authors": [{"family": "Dahmane", "given": "Selma", "initials": "S", "orcid": "0000-0003-4193-1496", "researcher": {"href": "https://publications.scilifelab.se/researcher/78124ba9dbac444aaee032c91c235a88.json"}}, {"family": "Schexnaydre", "given": "Erin", "initials": "E", "orcid": "0000-0002-2993-8647", "researcher": {"href": "https://publications.scilifelab.se/researcher/f643b7350ed5404aac418ce43d907f27.json"}}, {"family": "Zhang", "given": "Jianguo", "initials": "J", "orcid": "0000-0002-3784-6515", "researcher": {"href": "https://publications.scilifelab.se/researcher/3575f209f0d74bc1b8be39dd68d51fd3.json"}}, {"family": "Rosendal", "given": "Ebba", "initials": "E", "orcid": "0000-0001-8512-0535", "researcher": {"href": "https://publications.scilifelab.se/researcher/dda3f5ae552241eea145e011afdfad20.json"}}, {"family": "Chotiwan", "given": "Nunya", "initials": "N", "orcid": "0000-0003-3214-6605", "researcher": {"href": "https://publications.scilifelab.se/researcher/aba9da05817a4a8ea7037131000d6a7f.json"}}, {"family": "Singh", "given": "Bina Kumari", "initials": "BK", "orcid": "0000-0002-9500-2759", "researcher": {"href": "https://publications.scilifelab.se/researcher/ee00e03259f0463f84802861542cf940.json"}}, {"family": "Yau", "given": "Wai-Lok", "initials": "WL", "orcid": "0009-0007-0386-3919", "researcher": {"href": "https://publications.scilifelab.se/researcher/2044fb19cb8c4ecb94ddb6676da79a7d.json"}}, {"family": "Lundmark", "given": "Richard", "initials": "R", "orcid": "0000-0001-9104-724X", "researcher": {"href": "https://publications.scilifelab.se/researcher/3e1b756caa79468dab0f960e43cd61d3.json"}}, {"family": "Barad", "given": "Benjamin", "initials": "B", "orcid": "0000-0002-1016-862X", "researcher": {"href": "https://publications.scilifelab.se/researcher/8a118a58f2f140bab794786ac581d3ac.json"}}, {"family": "Grotjahn", "given": "Danielle A", "initials": "DA", "orcid": "0000-0001-5908-7882", "researcher": {"href": "https://publications.scilifelab.se/researcher/e9c2292eb2294f56bcc068a7a01737a8.json"}}, {"family": "Liese", "given": "Susanne", "initials": "S", "orcid": "0000-0001-7420-5488", "researcher": {"href": "https://publications.scilifelab.se/researcher/cb4ed3f41d6946e1a82472041e9a96d6.json"}}, {"family": "Carlson", "given": "Andreas", "initials": "A", "orcid": "0000-0002-3068-9983", "researcher": {"href": "https://publications.scilifelab.se/researcher/39f4cc3bec4747da984c7c382a8ca5ae.json"}}, {"family": "\u00d6verby", "given": "Anna K", "initials": "AK", "orcid": "0000-0001-6553-0940", "researcher": {"href": "https://publications.scilifelab.se/researcher/506b0e2b2d884f868df73c7663b9ffb7.json"}}, {"family": "Carlson", "given": "Lars-Anders", "initials": "LA", "orcid": "0000-0003-2342-6488", "researcher": {"href": "https://publications.scilifelab.se/researcher/ba0e366ce21e49b48212bbed5a0a7bd1.json"}}], "type": "journal article", "published": "2024-10-21", "journal": {"title": "bioRxiv", "issn": "2692-8205", "issn-l": null, "volume": null, "issue": null, "pages": null}, "abstract": "Flaviviruses replicate their genomes in replication organelles (ROs) formed as bud-like invaginations on the endoplasmic reticulum (ER) membrane, which also functions as the site for virion assembly. While this localization is well established, it is not known to what extent viral membrane remodeling, genome replication, virion assembly, and maturation are coordinated. Here, we imaged tick-borne flavivirus replication in human cells using cryo-electron tomography. We find that the RO membrane bud is shaped by a combination of a curvature-establishing coat and the pressure from intraluminal template RNA. A protein complex at the RO base extends to an adjacent membrane, where immature virions bud. Naturally occurring furin site variants determine whether virions mature in the immediate vicinity of ROs. We further visualize replication in mouse brain tissue by cryo-electron tomography. Taken together, these findings reveal a close spatial coupling of flavivirus genome replication, budding, and maturation.", "doi": "10.1101/2024.10.13.618056", "pmid": "39416041", "labels": {"Cryo-EM": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC11482891"}, {"db": "pii", "key": "2024.10.13.618056"}], "notes": [], "created": "2025-10-29T14:50:30.370Z", "modified": "2025-10-29T14:55:33.629Z"}]}