{"entity": "researcher", "timestamp": "2026-08-13T18:12:54.362Z", "family": "Heldin", "given": "Johan", "initials": "J", "orcid": "0000-0002-0915-5303", "affiliations": ["Department of Pharmaceutical Biosciences, Science for Life Laboratory, Uppsala University, Biomedical center, SE-751 24, Uppsala, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/d8a546798d014cd3a44537ae5db9f889.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/d8a546798d014cd3a44537ae5db9f889"}}, "publications": [{"entity": "publication", "iuid": "3a5f884be46740878875a12edfdc5a57", "links": {"self": {"href": "https://publications.scilifelab.se/publication/3a5f884be46740878875a12edfdc5a57.json"}, "display": {"href": "https://publications.scilifelab.se/publication/3a5f884be46740878875a12edfdc5a57"}}, "title": "Enzyme-activated Proximity of Oligonucleotides Sensing (EPOS), for precise monitoring of protein interactions", "authors": [{"family": "Heldin", "given": "Johan", "initials": "J", "orcid": "0000-0002-0915-5303", "researcher": {"href": "https://publications.scilifelab.se/researcher/d8a546798d014cd3a44537ae5db9f889.json"}}, {"family": "Wenson", "given": "Leonie", "initials": "L", "orcid": "0000-0002-1864-1258", "researcher": {"href": "https://publications.scilifelab.se/researcher/989e0534adbd426386cec5526c5e9668.json"}}, {"family": "Wenz", "given": "Amelie", "initials": "A", "orcid": "0000-0003-1929-382X", "researcher": {"href": "https://publications.scilifelab.se/researcher/113e59df51d0438c90dfef742c67fead.json"}}, {"family": "Dyck", "given": "Birte", "initials": "B"}, {"family": "Jonsson", "given": "Cajsa", "initials": "C", "orcid": "0009-0005-0276-7808", "researcher": {"href": "https://publications.scilifelab.se/researcher/938d54869a27480882293304c6fa4c52.json"}}, {"family": "Wagener", "given": "Luisa", "initials": "L"}, {"family": "W\u00e5hl\u00e9n", "given": "Erik", "initials": "E", "orcid": "0000-0003-1195-3539", "researcher": {"href": "https://publications.scilifelab.se/researcher/c6619714bd174e118d6368388cb4518d.json"}}, {"family": "Buratovic", "given": "Sonja", "initials": "S"}, {"family": "Sundqvist", "given": "Anders", "initials": "A"}, {"family": "Anbuhl", "given": "Stephanie", "initials": "S"}, {"family": "Heukers", "given": "Raimond", "initials": "R"}, {"family": "Paredes", "given": "Joana", "initials": "J"}, {"family": "Castro", "given": "Maria Miguel", "initials": "MM"}, {"family": "Leino", "given": "Matias", "initials": "M"}, {"family": "Larsson", "given": "Lars Gunnar", "initials": "LG"}, {"family": "Lennartsson", "given": "Johan", "initials": "J"}, {"family": "Puigvert", "given": "Jordi Carreras", "initials": "JC"}, {"family": "Koos", "given": "Bj\u00f6rn", "initials": "B"}, {"family": "S\u00f6derberg", "given": "Ola", "initials": "O", "orcid": "0000-0003-2883-1925", "researcher": {"href": "https://publications.scilifelab.se/researcher/68df823efa304c0b9962684ac1515808.json"}}], "type": "posted-content", "published": "2026-05-11", "journal": {"issn-l": null}, "abstract": null, "doi": "10.21203/rs.3.rs-9403735/v1", "pmid": null, "labels": {"Integrated Microscopy Technologies Stockholm": "Service"}, "xrefs": [], "notes": [], "created": "2026-08-11T11:27:22.671Z", "modified": "2026-08-11T11:27:22.956Z"}, {"entity": "publication", "iuid": "8cb122c7905e4043bd3f13414014d2fe", "links": {"self": {"href": "https://publications.scilifelab.se/publication/8cb122c7905e4043bd3f13414014d2fe.json"}, "display": {"href": "https://publications.scilifelab.se/publication/8cb122c7905e4043bd3f13414014d2fe"}}, "title": "The long noncoding RNA VIM-AS1 and nucleoporin Nup358/RanBP2 regulate SMAD nuclear accumulation during TGF-\u03b2 signaling.", "authors": [{"family": "Rodrigues-Junior", "given": "Dorival Mendes", "initials": "DM", "orcid": "0000-0002-8861-9240", "researcher": {"href": "https://publications.scilifelab.se/researcher/3334d6f77c1f42c2b10fd3e9bef23efb.json"}}, {"family": "Ali", "given": "Mohamad Moustafa", "initials": "MM"}, {"family": "Itoh", "given": "Yuka", "initials": "Y"}, {"family": "Ferreira", "given": "Mafalda Sousa", "initials": "MS"}, {"family": "Heldin", "given": "Johan", "initials": "J", "orcid": "0000-0002-0915-5303", "researcher": {"href": "https://publications.scilifelab.se/researcher/d8a546798d014cd3a44537ae5db9f889.json"}}, {"family": "Fu", "given": "Hao", "initials": "H"}, {"family": "Hoelz", "given": "Andr\u00e9", "initials": "A"}, {"family": "Heldin", "given": "Carl-Henrik", "initials": "CH"}, {"family": "Moustakas", "given": "Aristidis", "initials": "A", "orcid": "0000-0001-9131-3827", "researcher": {"href": "https://publications.scilifelab.se/researcher/6c1626d991f3485e81232db174537e6d.json"}}], "type": "journal article", "published": "2026-01-14", "journal": {"title": "Nucleic Acids Res.", "issn": "1362-4962", "volume": "54", "issue": "2", "issn-l": "0305-1048"}, "abstract": "The transforming growth factor \u03b2 (TGF-\u03b2) pathway is a developmental signaling network that regulates tissue homeostasis and malfunctions in human diseases, including cancer. TGF-\u03b2 signals via two receptors, which activate SMAD and alternative signaling pathways. We show that TGF-\u03b2 induces the expression of the mammalian long noncoding RNA (lncRNA) VIM-AS1 (Vimentin antisense RNA1) variant-2 (v.2) via a transcriptional SMAD-GATA6-SPI1 complex. VIM-AS1 v.1 and v.2 localize in different cell compartments, including the nuclear border. Unbiased whole transcriptomic analysis and functional gain and loss of function assays establish that VIM-AS1 v.2 enhances TGF-\u03b2 signaling. Mechanistically, VIM-AS1 v.2 interacts with the nucleoporin Nup358/RanBP2, contributing to the binding of Nup358/RanBP2 to SMAD2/3 and enhancing SMAD nuclear accumulation. In the context of cancer biology, VIM-AS1 did not affect the antiproliferative actions of TGF-\u03b2, yet had an impact on the epithelial-mesenchymal transition gene program, and increased the invasion and motility of tumor cells, whereas its silencing sensitized cancer cells to chemotherapeutic agents. The molecular mechanism highlights how a lncRNA can modulate the nuclear pore's capacity to import SMAD complexes, by facilitating their capture by Nup358/RanBP2 and thereby enhancing nuclear accumulation of SMADs with distinct isoform composition, thus promoting selectively TGF-\u03b2 signaling responses.", "doi": "10.1093/nar/gkaf1526", "pmid": "41556346", "labels": {"NGI Uppsala (SNP&SEQ Technology Platform)": "Service", "NGI Short read": "Service", "National Genomics Infrastructure": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC12817083"}, {"db": "pii", "key": "8431143"}], "notes": [], "created": "2026-06-01T11:10:58.548Z", "modified": "2026-06-01T11:10:58.802Z"}, {"entity": "publication", "iuid": "ab9b4fd3312a411fa7600da6cc9fc651", "links": {"self": {"href": "https://publications.scilifelab.se/publication/ab9b4fd3312a411fa7600da6cc9fc651.json"}, "display": {"href": "https://publications.scilifelab.se/publication/ab9b4fd3312a411fa7600da6cc9fc651"}}, "title": "Precise mapping of single-stranded DNA breaks by sequence-templated erroneous DNA polymerase end-labelling.", "authors": [{"family": "Wenson", "given": "Leonie", "initials": "L", "orcid": "0000-0002-1864-1258", "researcher": {"href": "https://publications.scilifelab.se/researcher/989e0534adbd426386cec5526c5e9668.json"}}, {"family": "Heldin", "given": "Johan", "initials": "J", "orcid": "0000-0002-0915-5303", "researcher": {"href": "https://publications.scilifelab.se/researcher/d8a546798d014cd3a44537ae5db9f889.json"}}, {"family": "Martin", "given": "Marcel", "initials": "M", "orcid": "0000-0002-0680-200X", "researcher": {"href": "https://publications.scilifelab.se/researcher/132afd4fea2e4e86bdf43708c8f49907.json"}}, {"family": "Erbilgin", "given": "Y\u00fccel", "initials": "Y"}, {"family": "Salman", "given": "Bar\u0131\u015f", "initials": "B", "orcid": "0000-0002-7657-8576", "researcher": {"href": "https://publications.scilifelab.se/researcher/eb919f7fcc794c8898fe800056d42f38.json"}}, {"family": "Sundqvist", "given": "Anders", "initials": "A"}, {"family": "Schaal", "given": "Wesley", "initials": "W", "orcid": "0000-0001-6770-0878", "researcher": {"href": "https://publications.scilifelab.se/researcher/ab184845a24f4effb22ec2b338ab8960.json"}}, {"family": "Sandbaumh\u00fcter", "given": "Friederike A", "initials": "FA"}, {"family": "Jansson", "given": "Erik T", "initials": "ET"}, {"family": "Chen", "given": "Xingqi", "initials": "X", "orcid": "0000-0002-5657-2839", "researcher": {"href": "https://publications.scilifelab.se/researcher/ef7ddc09e57745909175e41ac2d1b647.json"}}, {"family": "Davidsson", "given": "Anton", "initials": "A"}, {"family": "Stenerl\u00f6w", "given": "Bo", "initials": "B", "orcid": "0000-0001-8878-8071", "researcher": {"href": "https://publications.scilifelab.se/researcher/22437ff9315d43089232926973feb0d2.json"}}, {"family": "Espinoza", "given": "Jaime A", "initials": "JA", "orcid": "0000-0002-0731-2715", "researcher": {"href": "https://publications.scilifelab.se/researcher/3cdf2cd80f5b4f87adf6d936b6390ee8.json"}}, {"family": "Lindstr\u00f6m", "given": "Mikael", "initials": "M", "orcid": "0000-0003-1148-8497", "researcher": {"href": "https://publications.scilifelab.se/researcher/5aa942fbfbee4257a129b3e7888f5b6d.json"}}, {"family": "Lennartsson", "given": "Johan", "initials": "J"}, {"family": "Spjuth", "given": "Ola", "initials": "O", "orcid": "0000-0002-8083-2864", "researcher": {"href": "https://publications.scilifelab.se/researcher/605dbd52684d4e54ae4150a9933abe6e.json"}}, {"family": "S\u00f6derberg", "given": "Ola", "initials": "O", "orcid": "0000-0003-2883-1925", "researcher": {"href": "https://publications.scilifelab.se/researcher/68df823efa304c0b9962684ac1515808.json"}}], "type": "journal article", "published": "2025-08-04", "journal": {"title": "Nat Commun", "issn": "2041-1723", "issn-l": "2041-1723", "volume": "16", "issue": "1", "pages": "7130"}, "abstract": "The ability to analyze whether DNA contains lesions is essential in identifying mutagenic substances. Currently, the detection of single-stranded DNA breaks (SSBs) lacks precision. To address this limitation, we develop a method for sequence-templated erroneous end-labelling sequencing (STEEL-seq), which enables the mapping of SSBs. The method requires a highly error-prone DNA polymerase, so we engineer a chimeric DNA polymerase, Sloppymerase, capable of replicating DNA in the absence of one nucleotide. Following the omission of a specific nucleotide (e.g., dATP) from the reaction mixture, Sloppymerase introduces mismatches directly downstream of SSBs at positions where deoxyadenosine should occur. This mismatch pattern, coupled with the retention of sequence information flanking these sites, ensures that the identified hits are bona fide SSBs. STEEL-seq is compatible with a variety of sequencing technologies, as demonstrated using Sanger, Illumina, PacBio, and Nanopore systems. Using STEEL-seq, we determine the SSB/base pair frequency in the human genome to range between 0.7 and 3.8 \u00d7 10-6 with an enrichment in active promoter regions.", "doi": "10.1038/s41467-025-62512-4", "pmid": "40759655", "labels": {"NGI Short read": "Service", "National Genomics Infrastructure": "Service", "NGI Uppsala (Uppsala Genome Center)": "Service", "Bioinformatics Support, Infrastructure and Training": "Collaborative", "NGI Uppsala (SNP&SEQ Technology Platform)": "Service", "Bioinformatics (NBIS)": "Collaborative", "Bioinformatics Support and Infrastructure": "Collaborative", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC12322144"}, {"db": "pii", "key": "10.1038/s41467-025-62512-4"}], "notes": [], "created": "2025-08-19T13:16:23.958Z", "modified": "2025-11-28T10:45:45.544Z"}, {"entity": "publication", "iuid": "ce6cb2f40dc04d45a617214dd16e6f56", "links": {"self": {"href": "https://publications.scilifelab.se/publication/ce6cb2f40dc04d45a617214dd16e6f56.json"}, "display": {"href": "https://publications.scilifelab.se/publication/ce6cb2f40dc04d45a617214dd16e6f56"}}, "title": "Visualizing DNA single- and double-strand breaks in the Flash comet assay by DNA polymerase-assisted end-labelling.", "authors": [{"family": "Bivehed", "given": "Erik", "initials": "E", "orcid": "0000-0003-2471-1432", "researcher": {"href": "https://publications.scilifelab.se/researcher/93d2547de804478e8905ea0272edeea2.json"}}, {"family": "Hellman", "given": "Bj\u00f6rn", "initials": "B", "orcid": "0000-0002-3429-7856", "researcher": {"href": "https://publications.scilifelab.se/researcher/13986d919b7d49cfa75354c60563708c.json"}}, {"family": "Wenson", "given": "Leonie", "initials": "L"}, {"family": "Stenerl\u00f6w", "given": "Bo", "initials": "B"}, {"family": "S\u00f6derberg", "given": "Ola", "initials": "O", "orcid": "0000-0003-2883-1925", "researcher": {"href": "https://publications.scilifelab.se/researcher/68df823efa304c0b9962684ac1515808.json"}}, {"family": "Heldin", "given": "Johan", "initials": "J", "orcid": "0000-0002-0915-5303", "researcher": {"href": "https://publications.scilifelab.se/researcher/d8a546798d014cd3a44537ae5db9f889.json"}}], "type": "journal article", "published": "2024-02-28", "journal": {"title": "Nucleic Acids Res.", "issn": "1362-4962", "issn-l": "0305-1048", "volume": "52", "issue": "4", "pages": "e22"}, "abstract": "In the comet assay, tails are formed after single-cell gel electrophoresis if the cells have been exposed to genotoxic agents. These tails include a mixture of both DNA single-strand breaks (SSBs) and double-strand breaks (DSBs). However, these two types of strand breaks cannot be distinguished using comet assay protocols with conventional DNA stains. Since DSBs are more problematic for the cells, it would be useful if the SSBs and DSBs could be differentially identified in the same comet. In order to be able to distinguish between SSBs and DSBs, we designed a protocol for polymerase-assisted DNA damage analysis (PADDA) to be used in combination with the Flash comet protocol, or on fixed cells. By using DNA polymerase I to label SSBs and terminal deoxynucleotidyl transferase to label DSBs with fluorophore-labelled nucleotides. Herein, TK6-cells or HaCat cells were exposed to either hydrogen peroxide (H2O2), ionising radiation (X-rays) or DNA cutting enzymes, and then subjected to a comet protocol followed by PADDA. PADDA offers a wider detection range, unveiling previously undetected DNA strand breaks.", "doi": "10.1093/nar/gkae009", "pmid": "38261985", "labels": {"BioImage Informatics": "Service", "Bioinformatics (NBIS)": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC10899772"}, {"db": "pii", "key": "7585676"}], "notes": [], "created": "2024-11-13T09:56:48.375Z", "modified": "2024-11-13T09:56:58.931Z"}, {"entity": "publication", "iuid": "058e4ab4248b4f38bf463af3cb986da6", "links": {"self": {"href": "https://publications.scilifelab.se/publication/058e4ab4248b4f38bf463af3cb986da6.json"}, "display": {"href": "https://publications.scilifelab.se/publication/058e4ab4248b4f38bf463af3cb986da6"}}, "title": "A method for Boolean analysis of protein interactions at a molecular level.", "authors": [{"family": "Raykova", "given": "Doroteya", "initials": "D", "orcid": "0000-0001-6452-2199", "researcher": {"href": "https://publications.scilifelab.se/researcher/0a81c40491e349178167f148f2351875.json"}}, {"family": "Kermpatsou", "given": "Despoina", "initials": "D", "orcid": "0000-0001-5872-4472", "researcher": {"href": "https://publications.scilifelab.se/researcher/cfafe1fe67374c25ac5111ecd42c2fd4.json"}}, {"family": "Malmqvist", "given": "Tony", "initials": "T", "orcid": "0000-0003-0609-2009", "researcher": {"href": "https://publications.scilifelab.se/researcher/ee835b51354e4cb5aff5dfbf43964dc8.json"}}, {"family": "Harrison", "given": "Philip J", "initials": "PJ"}, {"family": "Sander", "given": "Marie Rubin", "initials": "MR", "orcid": "0000-0002-9783-5682", "researcher": {"href": "https://publications.scilifelab.se/researcher/68a774625f6948d693bf4ad09eaba273.json"}}, {"family": "Stiller", "given": "Christiane", "initials": "C", "orcid": "0000-0002-6552-8426", "researcher": {"href": "https://publications.scilifelab.se/researcher/2f8bb525e1094fbfbfb71dbbff62cbc7.json"}}, {"family": "Heldin", "given": "Johan", "initials": "J", "orcid": "0000-0002-0915-5303", "researcher": {"href": "https://publications.scilifelab.se/researcher/d8a546798d014cd3a44537ae5db9f889.json"}}, {"family": "Leino", "given": "Mattias", "initials": "M"}, {"family": "Ricardo", "given": "Sara", "initials": "S", "orcid": "0000-0003-4091-2226", "researcher": {"href": "https://publications.scilifelab.se/researcher/d5599dd22d9e44a5af16850baf14e10e.json"}}, {"family": "Klemm", "given": "Anna", "initials": "A", "orcid": "0000-0002-3466-1320", "researcher": {"href": "https://publications.scilifelab.se/researcher/4ae78afb2a424b0ab70d49871f361d13.json"}}, {"family": "David", "given": "Leonor", "initials": "L", "orcid": "0000-0003-4207-9258", "researcher": {"href": "https://publications.scilifelab.se/researcher/510030f8783044e8b644277783308bf6.json"}}, {"family": "Spjuth", "given": "Ola", "initials": "O", "orcid": "0000-0002-8083-2864", "researcher": {"href": "https://publications.scilifelab.se/researcher/605dbd52684d4e54ae4150a9933abe6e.json"}}, {"family": "Vemuri", "given": "Kalyani", "initials": "K", "orcid": "0000-0003-2544-5412", "researcher": {"href": "https://publications.scilifelab.se/researcher/b74c149757644aa5a0997af9ca583a45.json"}}, {"family": "Dimberg", "given": "Anna", "initials": "A", "orcid": "0000-0003-4422-9125", "researcher": {"href": "https://publications.scilifelab.se/researcher/c53166298a214331866c8cbf3bb9a3b9.json"}}, {"family": "Sundqvist", "given": "Anders", "initials": "A"}, {"family": "Norlin", "given": "Maria", "initials": "M", "orcid": "0000-0003-4348-6269", "researcher": {"href": "https://publications.scilifelab.se/researcher/a10ffe5af28b4398b8d19743f7927e34.json"}}, {"family": "Klaesson", "given": "Axel", "initials": "A"}, {"family": "Kampf", "given": "Caroline", "initials": "C"}, {"family": "S\u00f6derberg", "given": "Ola", "initials": "O", "orcid": "0000-0003-2883-1925", "researcher": {"href": "https://publications.scilifelab.se/researcher/68df823efa304c0b9962684ac1515808.json"}}], "type": "journal article", "published": "2022-08-13", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "13", "issue": "1", "pages": "4755", "issn-l": "2041-1723"}, "abstract": "Determining the levels of protein-protein interactions is essential for the analysis of signaling within the cell, characterization of mutation effects, protein function and activation in health and disease, among others. Herein, we describe MolBoolean - a method to detect interactions between endogenous proteins in various subcellular compartments, utilizing antibody-DNA conjugates for identification and signal amplification. In contrast to proximity ligation assays, MolBoolean simultaneously indicates the relative abundances of protein A and B not interacting with each other, as well as the pool of A and B proteins that are proximal enough to be considered an AB complex. MolBoolean is applicable both in fixed cells and tissue sections. The specific and quantifiable data that the method generates provide opportunities for both diagnostic use and medical research.", "doi": "10.1038/s41467-022-32395-w", "pmid": "35963857", "labels": {"BioImage Informatics": "Collaborative", "Bioinformatics (NBIS)": "Collaborative"}, "xrefs": [{"db": "pii", "key": "10.1038/s41467-022-32395-w"}, {"db": "pmc", "key": "PMC9375095"}], "notes": [], "created": "2022-08-30T09:04:56.787Z", "modified": "2022-08-30T09:04:57.234Z"}, {"entity": "publication", "iuid": "d16c2a4a4c114d26aed8e150867bcbbb", "links": {"self": {"href": "https://publications.scilifelab.se/publication/d16c2a4a4c114d26aed8e150867bcbbb.json"}, "display": {"href": "https://publications.scilifelab.se/publication/d16c2a4a4c114d26aed8e150867bcbbb"}}, "title": "Proximity-dependent initiation of hybridization chain reaction.", "authors": [{"family": "Koos", "given": "Bj\u00f6rn", "initials": "B"}, {"family": "Cane", "given": "Ga\u00eblle", "initials": "G"}, {"family": "Grannas", "given": "Karin", "initials": "K"}, {"family": "L\u00f6f", "given": "Liza", "initials": "L"}, {"family": "Arng\u00e5rden", "given": "Linda", "initials": "L"}, {"family": "Heldin", "given": "Johan", "initials": "J", "orcid": "0000-0002-0915-5303", "researcher": {"href": "https://publications.scilifelab.se/researcher/d8a546798d014cd3a44537ae5db9f889.json"}}, {"family": "Clausson", "given": "Carl-Magnus", "initials": "CM"}, {"family": "Klaesson", "given": "Axel", "initials": "A"}, {"family": "Hirvonen", "given": "M Karoliina", "initials": "MK"}, {"family": "de Oliveira", "given": "Felipe M S", "initials": "FM", "orcid": "0000-0001-6074-7462", "researcher": {"href": "https://publications.scilifelab.se/researcher/e21dfb5c78b94f9f8631d4bdba6cf9e6.json"}}, {"family": "Talibov", "given": "Vladimir O", "initials": "VO", "orcid": "0000-0002-1135-2744", "researcher": {"href": "https://publications.scilifelab.se/researcher/e2cdaf464ed342d695baf33c19690518.json"}}, {"family": "Pham", "given": "Nhan T", "initials": "NT"}, {"family": "Auer", "given": "Manfred", "initials": "M"}, {"family": "Danielson", "given": "U Helena", "initials": "UH", "orcid": "0000-0003-2728-0340", "researcher": {"href": "https://publications.scilifelab.se/researcher/b2bf7dffedf44237807c23718c72efa6.json"}}, {"family": "Haybaeck", "given": "Johannes", "initials": "J", "orcid": "0000-0001-5518-3359", "researcher": {"href": "https://publications.scilifelab.se/researcher/e7ed164711b3491489b91d7ecc35f63b.json"}}, {"family": "Kamali-Moghaddam", "given": "Masood", "initials": "M", "orcid": "0000-0002-1303-2218", "researcher": {"href": "https://publications.scilifelab.se/researcher/290dd535fb414c68bc49a8a2b7995770.json"}}, {"family": "S\u00f6derberg", "given": "Ola", "initials": "O", "orcid": "0000-0003-2883-1925", "researcher": {"href": "https://publications.scilifelab.se/researcher/68df823efa304c0b9962684ac1515808.json"}}], "type": "journal article", "published": "2015-06-12", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "6", "pages": "7294", "issn-l": "2041-1723"}, "abstract": "Sensitive detection of protein interactions and post-translational modifications of native proteins is a challenge for research and diagnostic purposes. A method for this, which could be used in point-of-care devices and high-throughput screening, should be reliable, cost effective and robust. To achieve this, here we design a method (proxHCR) that combines the need for proximal binding with hybridization chain reaction (HCR) for signal amplification. When two oligonucleotide hairpins conjugated to antibodies bind in close proximity, they can be activated to reveal an initiator sequence. This starts a chain reaction of hybridization events between a pair of fluorophore-labelled oligonucleotide hairpins, generating a fluorescent product. In conclusion, we show the applicability of the proxHCR method for the detection of protein interactions and posttranslational modifications in microscopy and flow cytometry. As no enzymes are needed, proxHCR may be an inexpensive and robust alternative to proximity ligation assays.", "doi": "10.1038/ncomms8294", "pmid": "26065580", "labels": {"PLA and Single Cell Proteomics": "Service", "Affinity Proteomics Uppsala": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC4490387"}, {"db": "pii", "key": "ncomms8294"}], "notes": [], "created": "2023-06-16T12:43:37.596Z", "modified": "2023-06-16T12:44:14.437Z"}]}