{"entity": "researcher", "timestamp": "2026-06-13T15:42:46.568Z", "family": "\u00d6berg", "given": "Rasmus", "initials": "R", "orcid": "0000-0002-0168-0197", "affiliations": ["Swedish Defence Research Agency (FOI), Ume\u00e5 90621, Sweden.", "Department of Physics, Ume\u00e5 University, Ume\u00e5 90736, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/d23b2e0213244a598ab42d29c9dbea24.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/d23b2e0213244a598ab42d29c9dbea24"}}, "publications": [{"entity": "publication", "iuid": "e65ee69679804c9db4f4b460585bd4d7", "links": {"self": {"href": "https://publications.scilifelab.se/publication/e65ee69679804c9db4f4b460585bd4d7.json"}, "display": {"href": "https://publications.scilifelab.se/publication/e65ee69679804c9db4f4b460585bd4d7"}}, "title": "Ultra-Sensitive Detection of Bacterial Spores via SERS.", "authors": [{"family": "Segervald", "given": "Jonas", "initials": "J", "orcid": "0009-0002-9248-5748", "researcher": {"href": "https://publications.scilifelab.se/researcher/283c709a8ce74c118e3e1710814fb018.json"}}, {"family": "Malyshev", "given": "Dmitry", "initials": "D", "orcid": "0000-0002-0496-6692", "researcher": {"href": "https://publications.scilifelab.se/researcher/a36e950e3dd143339cf3d246a63ab117.json"}}, {"family": "\u00d6berg", "given": "Rasmus", "initials": "R", "orcid": "0000-0002-0168-0197", "researcher": {"href": "https://publications.scilifelab.se/researcher/d23b2e0213244a598ab42d29c9dbea24.json"}}, {"family": "Z\u00e4ll", "given": "Erik", "initials": "E"}, {"family": "Jia", "given": "Xueen", "initials": "X"}, {"family": "W\u00e5gberg", "given": "Thomas", "initials": "T", "orcid": "0000-0002-5080-8273", "researcher": {"href": "https://publications.scilifelab.se/researcher/1614ac6527654be8b0e15fd079cb4233.json"}}, {"family": "Andersson", "given": "Magnus", "initials": "M", "orcid": "0000-0002-9835-3263", "researcher": {"href": "https://publications.scilifelab.se/researcher/fe860af5d944447086cbf2d00e3beb3e.json"}}], "type": "journal article", "published": "2025-02-28", "journal": {"title": "ACS Sens.", "issn": "2379-3694", "volume": "10", "issue": "2", "pages": "1237-1248", "issn-l": "2379-3694"}, "abstract": "Bacterial spores are highly resilient and capable of surviving extreme conditions, making them a persistent threat in contexts such as disease transmission, food safety, and bioterrorism. Their ability to withstand conventional sterilization methods necessitates rapid and accurate detection techniques to effectively mitigate the risks they present. In this study, we introduce a surface-enhanced Raman spectroscopy (SERS) approach for detecting Bacillus thuringiensis spores by targeting calcium dipicolinate acid (CaDPA), a biomarker uniquely associated with bacterial spores. Our method uses probe sonication to disrupt spores, releasing their CaDPA, which is then detected by SERS on drop-dried supernatant mixed with gold nanorods. This simple approach enables the selective detection of CaDPA, distinguishing it from other spore components and background noise. We demonstrate detection of biogenic CaDPA from concentrations as low as 103 spores/mL, with sensitivity reaching beyond CaDPA levels of a single spore. Finally, we show the method's robustness by detecting CaDPA from a realistic sample of fresh milk mixed with spores. These findings highlight the potential of SERS as a sensitive and specific technique for bacterial spore detection, with implications for fields requiring rapid and reliable spore identification.", "doi": "10.1021/acssensors.4c03151", "pmid": "39847439", "labels": {"Integrated Microscopy Technologies Ume\u00e5": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11877637"}], "notes": [], "created": "2025-01-30T11:37:25.468Z", "modified": "2025-11-03T12:41:02.712Z"}, {"entity": "publication", "iuid": "febccaa6498f4dedbc33584c79392397", "links": {"self": {"href": "https://publications.scilifelab.se/publication/febccaa6498f4dedbc33584c79392397.json"}, "display": {"href": "https://publications.scilifelab.se/publication/febccaa6498f4dedbc33584c79392397"}}, "title": "UV-Induced Spectral and Morphological Changes in Bacterial Spores for Inactivation Assessment.", "authors": [{"family": "\u00d6berg", "given": "Rasmus", "initials": "R", "orcid": "0000-0002-0168-0197", "researcher": {"href": "https://publications.scilifelab.se/researcher/d23b2e0213244a598ab42d29c9dbea24.json"}}, {"family": "Sil", "given": "Timir B", "initials": "TB"}, {"family": "Johansson", "given": "Alexandra C", "initials": "AC"}, {"family": "Malyshev", "given": "Dmitry", "initials": "D", "orcid": "0000-0002-0496-6692", "researcher": {"href": "https://publications.scilifelab.se/researcher/a36e950e3dd143339cf3d246a63ab117.json"}}, {"family": "Landstr\u00f6m", "given": "Lars", "initials": "L"}, {"family": "Johansson", "given": "Susanne", "initials": "S", "orcid": "0000-0002-2298-7008", "researcher": {"href": "https://publications.scilifelab.se/researcher/1b957ea589b342fb9def8ce13ef3bd7e.json"}}, {"family": "Andersson", "given": "Magnus", "initials": "M", "orcid": "0000-0002-9835-3263", "researcher": {"href": "https://publications.scilifelab.se/researcher/fe860af5d944447086cbf2d00e3beb3e.json"}}, {"family": "Andersson", "given": "Per Ola", "initials": "PO", "orcid": "0000-0002-1253-4283", "researcher": {"href": "https://publications.scilifelab.se/researcher/7f49a909b66640c697e5e7af7635b820.json"}}], "type": "journal article", "published": "2024-02-22", "journal": {"title": "J. Phys. Chem. B", "issn": "1520-5207", "issn-l": null, "volume": "128", "issue": "7", "pages": "1638-1646"}, "abstract": "The ability to detect and inactivate spore-forming bacteria is of significance within, for example, industrial, healthcare, and defense sectors. Not only are stringent protocols necessary for the inactivation of spores but robust procedures are also required to detect viable spores after an inactivation assay to evaluate the procedure's success. UV radiation is a standard procedure to inactivate spores. However, there is limited understanding regarding its impact on spores' spectral and morphological characteristics. A further insight into these UV-induced changes can significantly improve the design of spore decontamination procedures and verification assays. This work investigates the spectral and morphological changes to Bacillus thuringiensis spores after UV exposure. Using absorbance and fluorescence spectroscopy, we observe an exponential decay in the spectral intensity of amino acids and protein structures, as well as a logistic increase in dimerized DPA with increased UV exposure on bulk spore suspensions. Additionally, using micro-Raman spectroscopy, we observe DPA release and protein degradation with increased UV exposure. More specifically, the protein backbone's 1600-1700 cm-1 amide I band decays slower than other amino acid-based structures. Last, using electron microscopy and light scattering measurements, we observe shriveling of the spore bodies with increased UV radiation, alongside the leaking of core content and disruption of proteinaceous coat and exosporium layers. Overall, this work utilized spectroscopy and electron microscopy techniques to gain new understanding of UV-induced spore inactivation relating to spore degradation and CaDPA release. The study also identified spectroscopic indicators that can be used to determine spore viability after inactivation. These findings have practical applications in the development of new spore decontamination and inactivation validation methods.", "doi": "10.1021/acs.jpcb.3c07062", "pmid": "38326108", "labels": {"Integrated Microscopy Technologies Ume\u00e5": "Service", "Cryo-EM": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC10895659"}], "notes": [], "created": "2024-11-05T10:54:54.165Z", "modified": "2025-11-04T09:35:53.654Z"}]}