{"entity": "researcher", "timestamp": "2026-07-17T14:56:41.441Z", "family": "\u017demaitis", "given": "Kristijonas", "initials": "K", "orcid": "0000-0002-4098-0184", "affiliations": ["Molecular Medicine and Gene Therapy, Lund Stem Cell Center, Lund University, Lund, Sweden;"], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/d122126ea9094e1ab3c83a2265175042.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/d122126ea9094e1ab3c83a2265175042"}}, "publications": [{"entity": "publication", "iuid": "f610248a3f26412e8d529592c0b43cd1", "links": {"self": {"href": "https://publications.scilifelab.se/publication/f610248a3f26412e8d529592c0b43cd1.json"}, "display": {"href": "https://publications.scilifelab.se/publication/f610248a3f26412e8d529592c0b43cd1"}}, "title": "Ciclopirox Ethanolamine Preserves the Immature State of Human HSCs by Mediating Intracellular Iron Content.", "authors": [{"family": "Safaee Talkhoncheh", "given": "Mehrnaz", "initials": "M", "orcid": "0000-0001-8429-7566", "researcher": {"href": "https://publications.scilifelab.se/researcher/5e8e20a05c6b4af9b37e0b15ebddae0e.json"}}, {"family": "Baudet", "given": "Aur\u00e9lie", "initials": "A", "orcid": "0000-0002-3246-1819", "researcher": {"href": "https://publications.scilifelab.se/researcher/b221093f801845bf8739ae7876878643.json"}}, {"family": "Ek", "given": "Fredrik", "initials": "F"}, {"family": "Subramaniam", "given": "Agatheeswaran", "initials": "A", "orcid": "0000-0003-3966-3875", "researcher": {"href": "https://publications.scilifelab.se/researcher/5ccb1e6798b84f128c3143c8da8bd35a.json"}}, {"family": "Kao", "given": "Yun-Ruei", "initials": "YR"}, {"family": "Miharada", "given": "Natsumi", "initials": "N"}, {"family": "Karlsson", "given": "Christine", "initials": "C"}, {"family": "Oburoglu", "given": "Leal", "initials": "L", "orcid": "0000-0003-0130-6602", "researcher": {"href": "https://publications.scilifelab.se/researcher/cac8186ea00246e6a07f421e492041e6.json"}}, {"family": "Rydstr\u00f6m", "given": "Anna", "initials": "A", "orcid": "0000-0002-3404-7195", "researcher": {"href": "https://publications.scilifelab.se/researcher/1c074907dfd84c9c9d90a21fe426f1af.json"}}, {"family": "\u017demaitis", "given": "Kristijonas", "initials": "K", "orcid": "0000-0002-4098-0184", "researcher": {"href": "https://publications.scilifelab.se/researcher/d122126ea9094e1ab3c83a2265175042.json"}}, {"family": "Alattar", "given": "Abdul Ghani", "initials": "AG", "orcid": "0000-0002-0319-4416", "researcher": {"href": "https://publications.scilifelab.se/researcher/28c63e788c764cdd9c88ae3317d91ab0.json"}}, {"family": "Rak", "given": "Justyna", "initials": "J"}, {"family": "Pietras", "given": "Kristian", "initials": "K", "orcid": "0000-0001-6738-4705", "researcher": {"href": "https://publications.scilifelab.se/researcher/5be0a3ec07654822a91df964eab1d6e4.json"}}, {"family": "Olsson", "given": "Roger", "initials": "R", "orcid": "0000-0002-7107-3472", "researcher": {"href": "https://publications.scilifelab.se/researcher/f3e5a72515bf44e98f43d4690c6e577e.json"}}, {"family": "Will", "given": "Britta", "initials": "B", "orcid": "0000-0002-9534-6690", "researcher": {"href": "https://publications.scilifelab.se/researcher/dab18fe025354ada97f7f186eb48439b.json"}}, {"family": "Larsson", "given": "Jonas", "initials": "J"}], "type": "journal article", "published": "2023-07-24", "journal": {"title": "Blood Adv", "issn": "2473-9537", "issn-l": "2473-9529"}, "abstract": "Culture conditions in which hematopoietic stem cells (HSCs) can be expanded for clinical benefit are highly sought after. To elucidate regulatory mechanisms governing the maintenance and propagation of human HSCs ex vivo, we screened libraries of annotated small molecules in human cord blood (CB) cells using an optimized assay for detection of functional HSCs during culture. We found that the antifungal agent ciclopirox ethanolamine (CPX) selectively supported immature CD34+CD90+ cells during culture and enhanced their long-term in vivo repopulation capacity. Purified HSCs treated with CPX showed a reduced cell division rate and an enrichment of HSC- specific gene expression patterns. Mechanistically, we found that the HSC stimulating effect of CPX was directly mediated by chelation of the intracellular iron pool, which in turn affected iron-dependent proteins and enzymes mediating cellular metabolism and respiration. Our findings unveil a significant impact of iron homeostasis in regulation of human HSCs, with important implications for both basic HSC biology and clinical hematology.", "doi": "10.1182/bloodadvances.2023009844", "pmid": "37487020", "labels": {"Chemical Biology Consortium Sweden": "Collaborative"}, "xrefs": [{"db": "pii", "key": "497023"}], "notes": [], "created": "2023-11-24T11:40:56.111Z", "modified": "2025-10-17T13:04:27.619Z"}, {"entity": "publication", "iuid": "7d82542687dd4fccb7affffc780d00ec", "links": {"self": {"href": "https://publications.scilifelab.se/publication/7d82542687dd4fccb7affffc780d00ec.json"}, "display": {"href": "https://publications.scilifelab.se/publication/7d82542687dd4fccb7affffc780d00ec"}}, "title": "Lysine-specific demethylase 1A restricts ex vivo propagation of human HSCs and is a target of UM171.", "authors": [{"family": "Subramaniam", "given": "Agatheeswaran", "initials": "A", "orcid": "0000-0003-3966-3875", "researcher": {"href": "https://publications.scilifelab.se/researcher/5ccb1e6798b84f128c3143c8da8bd35a.json"}}, {"family": "\u017demaitis", "given": "Kristijonas", "initials": "K", "orcid": "0000-0002-4098-0184", "researcher": {"href": "https://publications.scilifelab.se/researcher/d122126ea9094e1ab3c83a2265175042.json"}}, {"family": "Talkhoncheh", "given": "Mehrnaz Safaee", "initials": "MS"}, {"family": "Yudovich", "given": "David", "initials": "D", "orcid": "0000-0002-6580-0637", "researcher": {"href": "https://publications.scilifelab.se/researcher/e88d5ea329514c1881c5be9d9ab14412.json"}}, {"family": "B\u00e4ckstr\u00f6m", "given": "Alexandra", "initials": "A", "orcid": "0000-0003-2926-4221", "researcher": {"href": "https://publications.scilifelab.se/researcher/8032ff5c71544155978bfc1c73b34848.json"}}, {"family": "Debnath", "given": "Shubhranshu", "initials": "S"}, {"family": "Chen", "given": "Jun", "initials": "J", "orcid": "0000-0001-8516-0975", "researcher": {"href": "https://publications.scilifelab.se/researcher/93cf6eaa8a61475789a772d30f29d2e5.json"}}, {"family": "Jain", "given": "Mayur Vilas", "initials": "MV"}, {"family": "Galeev", "given": "Roman", "initials": "R"}, {"family": "Gaetani", "given": "Massimiliano", "initials": "M", "orcid": "0000-0001-5610-0797", "researcher": {"href": "https://publications.scilifelab.se/researcher/7b58e5cef5224fdcbdcd626fb798b169.json"}}, {"family": "Zubarev", "given": "Roman A", "initials": "RA", "orcid": "0000-0001-9839-2089", "researcher": {"href": "https://publications.scilifelab.se/researcher/e971b9cdec2b4411934f9c5d535da8b4.json"}}, {"family": "Larsson", "given": "Jonas", "initials": "J"}], "type": "journal article", "published": "2020-11-05", "journal": {"title": "Blood", "issn": "1528-0020", "issn-l": "0006-4971", "volume": "136", "issue": "19", "pages": "2151-2161"}, "abstract": "Culture conditions in which hematopoietic stem cells (HSCs) can be expanded for clinical benefit are highly sought after. Here, we report that inhibition of the epigenetic regulator lysine-specific histone demethylase 1A (LSD1) induces a rapid expansion of human cord blood-derived CD34+ cells and promotes in vitro propagation of long-term repopulating HSCs by preventing differentiation. The phenotype and molecular characteristics of cells treated with LSD1 inhibitors were highly similar to cells treated with UM171, an agent promoting expansion of HSCs through undefined mechanisms and currently being tested in clinical trials. Strikingly, we found that LSD1, as well as other members of the LSD1-containing chromatin remodeling complex CoREST, is rapidly polyubiquitinated and degraded upon UM171 treatment. CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 depletion of the CoREST core member, RCOR1, resulted in expansion of CD34+ cells similar to LSD1 inhibition and UM171. Taken together, LSD1 and CoREST restrict HSC expansion and are principal targets of UM171, forming a mechanistic basis for the HSC-promoting activity of UM171.", "doi": "10.1182/blood.2020005827", "pmid": "32582923", "labels": {"Chemical Proteomics": "Collaborative", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pii", "key": "S0006-4971(21)00492-4"}, {"db": "pmc", "key": "PMC7645986"}], "notes": [], "created": "2020-07-01T11:45:41.535Z", "modified": "2024-01-18T23:37:23.868Z"}]}