{"entity": "researcher", "timestamp": "2026-07-20T00:28:07.997Z", "family": "von Grothusen", "given": "Carolina", "initials": "C", "orcid": "0000-0002-1508-8952", "affiliations": ["Department of Women's and Children's Health, Division of Obstetrics and Gynecology, Karolinska Institutet, and Karolinska University Hospital, S-171 64 Solna, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/d114195666f34b3ab78b054a13ceede1.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/d114195666f34b3ab78b054a13ceede1"}}, "publications": [{"entity": "publication", "iuid": "6e36dcb6fc944e67ba802034b10f625c", "links": {"self": {"href": "https://publications.scilifelab.se/publication/6e36dcb6fc944e67ba802034b10f625c.json"}, "display": {"href": "https://publications.scilifelab.se/publication/6e36dcb6fc944e67ba802034b10f625c"}}, "title": "Uterine fluid microRNAs are dysregulated in women with recurrent implantation failure.", "authors": [{"family": "von Grothusen", "given": "Carolina", "initials": "C", "orcid": "0000-0002-1508-8952", "researcher": {"href": "https://publications.scilifelab.se/researcher/d114195666f34b3ab78b054a13ceede1.json"}}, {"family": "Frisendahl", "given": "Caroline", "initials": "C", "orcid": "0000-0001-5283-6692", "researcher": {"href": "https://publications.scilifelab.se/researcher/bc410e454e8d4eec949737d3f29d945a.json"}}, {"family": "Modhukur", "given": "Vijayachitra", "initials": "V"}, {"family": "Lalitkumar", "given": "Parameswaran Grace", "initials": "PG", "orcid": "0000-0001-6733-533X", "researcher": {"href": "https://publications.scilifelab.se/researcher/ce39a89ff7c2470f9333cc5bcf9ad412.json"}}, {"family": "Peters", "given": "Maire", "initials": "M"}, {"family": "Faridani", "given": "Omid R", "initials": "OR"}, {"family": "Salumets", "given": "Andres", "initials": "A", "orcid": "0000-0002-1251-8160", "researcher": {"href": "https://publications.scilifelab.se/researcher/88dcf4bacf5c4792bbfd111495d43595.json"}}, {"family": "Boggavarapu", "given": "Nageswara Rao", "initials": "NR"}, {"family": "Gemzell-Danielsson", "given": "Kristina", "initials": "K", "orcid": "0000-0001-6516-1444", "researcher": {"href": "https://publications.scilifelab.se/researcher/44544a9f401c4db8a5cbdeca3d31e4b2.json"}}], "type": "journal article", "published": "2022-04-01", "journal": {"title": "Hum. Reprod.", "issn": "1460-2350", "issn-l": "0268-1161", "volume": "37", "issue": "4", "pages": "734-746"}, "abstract": "Is the composition of microRNAs (miRNAs) in uterine fluid (UF) of women with recurrent implantation failure (RIF) different from that of healthy fertile women?\r\n\r\nThe composition of miRNAs in UF of women with RIF is different from that of healthy fertile women and the dysregulated miRNAs are associated with impaired endometrial receptivity and embryo implantation.\r\n\r\nIt has previously been demonstrated that the miRNAs secreted from endometrial cells into the UF contribute to the achievement of endometrial receptivity. Endometrial miRNAs are dysregulated in women with RIF.\r\n\r\nIn this descriptive laboratory case-control study, miRNA abundancy was compared between UF collected during implantation phase from healthy fertile women (n = 17) and women with RIF (n = 34), which was defined as three failed IVF cycles with high-quality embryos.\r\n\r\nRecruitment of study subjects and sampling of UF were performed at two university clinics in Stockholm, Sweden and Tartu, Estonia. The study participants monitored their menstrual cycles using an LH test kit. The UF samples were collected on Day LH + 7-9 by flushing with saline. Samples were processed for small RNA sequencing and mapped for miRNAs. The differential abundance of miRNAs in UF was compared between the two groups using differential expression analysis (DESeq2). Further downstream analyses, including miRNA target gene prediction (miRTarBase), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis (g:Profiler) and external validation using relevant published data, were performed on the dysregulated miRNAs. Two miRNAs were technically validated with quantitative real-time PCR (RT-PCR).\r\n\r\nAfter processing of the sequencing data, there were 15 samples in the healthy fertile group and 33 samples in the RIF group. We found 61 differentially abundant UF miRNAs (34 upregulated and 27 downregulated) in RIF compared to healthy women with a false discovery rate of <0.05 and a fold change (FC) of \u2264-2 or \u22652. When analyzed with published literature, we found that several of the differentially abundant miRNAs are expressed in endometrial epithelial cells and have been reported in endometrial extracellular vesicles and in association with endometrial receptivity and RIF. Their predicted target genes were further expressed both in the trophectodermal cells of blastocyst-stage embryos and endometrial mid-secretory epithelial cells, as assessed by publicly available single-cell transcriptome-sequencing studies. Pathway analysis further revealed that 25 pathways, having key roles in endometrial receptivity and implantation, were significantly enriched. Hsa-miR-486-5p (FC -20.32; P-value = 0.004) and hsa-miR-92b-3p (FC -9.72; P-value = 0.004) were successfully technically validated with RT-PCR.\r\n\r\nThe data are available in Gene Expression Omnibus (GEO) at https://www.ncbi.nlm.nih.gov/geo/ with GEO accession number: GSE173289.\r\n\r\nThis is a descriptive study with a limited number of study participants. Moreover, the identified differentially abundant miRNAs should be validated in a larger study cohort, and the predicted miRNA target genes and enriched pathways in RIF need to be confirmed and further explored in vitro.\r\n\r\nRIF is a major challenge in the current IVF setting with no diagnostic markers nor effective treatment options at hand. For the first time, total miRNAs have been extensively mapped in receptive phase UF of both healthy women with proven fertility and women diagnosed with RIF. Our observations shed further light on the molecular mechanisms behind RIF, with possible implications in future biomarker and clinical treatment studies.\r\n\r\nThis work was financially supported by the Swedish Research Council (2017-00932), a joint grant from Region Stockholm and Karolinska Institutet (ALF Medicine 2020, FoUI-954072), Estonian Research Council (PRG1076), Horizon 2020 innovation (ERIN, EU952516) and European Commission and Enterprise Estonia (EU48695). The authors have no competing interests to declare for the current study.", "doi": "10.1093/humrep/deac019", "pmid": "35147192", "labels": {"NGI Single cell": "Service", "NGI Stockholm (Genomics Applications)": "Service", "NGI Stockholm (Genomics Production)": "Service", "National Genomics Infrastructure": "Service", "NGI Short read": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC8971651"}, {"db": "pii", "key": "6526798"}], "notes": [], "created": "2022-12-01T13:48:42.298Z", "modified": "2024-01-16T13:48:37.141Z"}, {"entity": "publication", "iuid": "ff762513ebb04c45bf11963125687232", "links": {"self": {"href": "https://publications.scilifelab.se/publication/ff762513ebb04c45bf11963125687232.json"}, "display": {"href": "https://publications.scilifelab.se/publication/ff762513ebb04c45bf11963125687232"}}, "title": "Stromal Heterogeneity in the Human Proliferative Endometrium-A Single-Cell RNA Sequencing Study.", "authors": [{"family": "Queckb\u00f6rner", "given": "Suzanna", "initials": "S"}, {"family": "von Grothusen", "given": "Carolina", "initials": "C", "orcid": "0000-0002-1508-8952", "researcher": {"href": "https://publications.scilifelab.se/researcher/d114195666f34b3ab78b054a13ceede1.json"}}, {"family": "Boggavarapu", "given": "Nageswara Rao", "initials": "NR"}, {"family": "Francis", "given": "Roy Mathew", "initials": "RM"}, {"family": "Davies", "given": "Lindsay C", "initials": "LC"}, {"family": "Gemzell-Danielsson", "given": "Kristina", "initials": "K", "orcid": "0000-0001-6516-1444", "researcher": {"href": "https://publications.scilifelab.se/researcher/44544a9f401c4db8a5cbdeca3d31e4b2.json"}}], "type": "journal article", "published": "2021-05-22", "journal": {"title": "J Pers Med", "issn": "2075-4426", "volume": "11", "issue": "6", "issn-l": null}, "abstract": "The endometrium undergoes regular regeneration and stromal proliferation as part of the normal menstrual cycle. To better understand cellular interactions driving the mechanisms in endometrial regeneration we employed single-cell RNA sequencing. Endometrial biopsies were obtained during the proliferative phase of the menstrual cycle from healthy fertile women and processed to single-cell suspensions which were submitted for sequencing. In addition to known endometrial cell types, bioinformatic analysis revealed multiple stromal populations suggestive of specific stromal niches with the ability to control inflammation and extracellular matrix composition. Ten different stromal cells and two pericyte subsets were identified. Applying different R packages (Seurat, SingleR, Velocyto) we established cell cluster diversity and cell lineage/trajectory, while using external data to validate our findings. By understanding healthy regeneration in the described stromal compartments, we aim to identify points of further investigation and possible targets for novel therapy development for benign gynecological disorders affecting endometrial regeneration and proliferation such as endometriosis and Asherman's syndrome.", "doi": "10.3390/jpm11060448", "pmid": "34067358", "labels": {"Eukaryotic Single Cell Genomics (ESCG)": "Service", "Bioinformatics Support, Infrastructure and Training": "Collaborative", "Bioinformatics Support and Infrastructure": "Collaborative", "Bioinformatics Support for Computational Resources": "Service", "Bioinformatics (NBIS)": "Collaborative"}, "xrefs": [{"db": "pii", "key": "jpm11060448"}, {"db": "pmc", "key": "PMC8224746"}], "notes": [], "created": "2021-12-02T13:20:38.533Z", "modified": "2024-01-16T13:48:39.689Z"}]}