{"entity": "researcher", "timestamp": "2026-07-20T22:57:15.151Z", "family": "Gillet", "given": "Natacha", "initials": "N", "orcid": "0000-0002-7657-6861", "affiliations": ["ENS de Lyon, CNRS, Universit\u00e9 Claude Bernard Lyon 1, Laboratoire de Chimie UMR 5182, F-69342 Lyon, France."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/ce5b43ffd5e44fbe926a3d26064b6760.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/ce5b43ffd5e44fbe926a3d26064b6760"}}, "publications": [{"entity": "publication", "iuid": "a537001d14d34f14b3048bdf35e0b950", "links": {"self": {"href": "https://publications.scilifelab.se/publication/a537001d14d34f14b3048bdf35e0b950.json"}, "display": {"href": "https://publications.scilifelab.se/publication/a537001d14d34f14b3048bdf35e0b950"}}, "title": "Mechanistic insights into PCBP1-driven unfolding of selected i-motif DNA at G1/S checkpoint.", "authors": [{"family": "Sengupta", "given": "Pallabi", "initials": "P", "orcid": "0000-0002-1413-9412", "researcher": {"href": "https://publications.scilifelab.se/researcher/851c95f648f242e0ba67202279725796.json"}}, {"family": "Gillet", "given": "Natacha", "initials": "N", "orcid": "0000-0002-7657-6861", "researcher": {"href": "https://publications.scilifelab.se/researcher/ce5b43ffd5e44fbe926a3d26064b6760.json"}}, {"family": "Obi", "given": "Ikenna", "initials": "I", "orcid": "0000-0003-0364-8964", "researcher": {"href": "https://publications.scilifelab.se/researcher/e46c65e7b0e540b0a7c225d54c1502d7.json"}}, {"family": "Sabouri", "given": "Nasim", "initials": "N", "orcid": "0000-0002-4541-7702", "researcher": {"href": "https://publications.scilifelab.se/researcher/4bdc688dc85a4932acfdfffad8bfc443.json"}}], "type": "journal article", "published": "2026-02-02", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "17", "issue": "1", "pages": "1149", "issn-l": "2041-1723"}, "abstract": "I-motifs are non-canonical, four-stranded DNA structures in cytosine-rich genomic regions, yet their protein-mediated regulation remains underexplored. Here, we identify PCBP1 (Poly(rC)-binding protein 1) as a selective i-motif-binding protein that unfolds specific i-motifs depending on their protonation and hairpin-forming propensities. Systematic truncation reveals that individual K-homology (KH) domains of PCBP1 cannot selectively bind or unfold i-motifs, but their coordinated actions restore wild-type PCBP1 functions. Using biochemical, biophysical, and molecular dynamics studies, we demonstrate that KH1+2 domains remodel i-motifs, recruiting KH3 to facilitate unfolding and efficient DNA replication. Chromatin and cell-based investigations reveal that PCBP1-knockdown increases i-motif formation at specific genomic loci, coinciding with G1/S arrest and elevated \u03b3H2AX, indicative of genomic instability. During G1/S transition, PCBP1 occupancy peaks at these i-motif loci, ensuring i-motif resolution in early S phase. These findings establish PCBP1 as a critical regulator of i-motif dynamics, directly linking its unfolding activity to G1/S transition and genome stability.", "doi": "10.1038/s41467-026-68822-5", "pmid": "41629296", "labels": {"Swedish NMR Centre": "Service"}, "xrefs": [{"db": "pii", "key": "10.1038/s41467-026-68822-5"}, {"db": "pmc", "key": "PMC12865031"}], "notes": [], "created": "2026-02-04T15:32:08.919Z", "modified": "2026-02-04T15:32:09.419Z"}, {"entity": "publication", "iuid": "b174f9dfdb4a4fc693ef7617a4b88f0f", "links": {"self": {"href": "https://publications.scilifelab.se/publication/b174f9dfdb4a4fc693ef7617a4b88f0f.json"}, "display": {"href": "https://publications.scilifelab.se/publication/b174f9dfdb4a4fc693ef7617a4b88f0f"}}, "title": "A new G-quadruplex-specific photosensitizer inducing genome instability in cancer cells by triggering oxidative DNA damage and impeding replication fork progression.", "authors": [{"family": "Deiana", "given": "Marco", "initials": "M", "orcid": "0000-0002-7815-4494", "researcher": {"href": "https://publications.scilifelab.se/researcher/f9efbe74ca3940029d782d3ba645fa34.json"}}, {"family": "Andr\u00e9s Cast\u00e1n", "given": "Jos\u00e9 Mar\u00eda", "initials": "JM", "orcid": "0000-0003-1012-9505", "researcher": {"href": "https://publications.scilifelab.se/researcher/72c1e687626d479bb738b734ddd5ffed.json"}}, {"family": "Josse", "given": "Pierre", "initials": "P", "orcid": "0000-0002-4943-5828", "researcher": {"href": "https://publications.scilifelab.se/researcher/cfa046fb387846e083ce32f9ae3914d9.json"}}, {"family": "Kahsay", "given": "Abraha", "initials": "A"}, {"family": "S\u00e1nchez", "given": "Dar\u00edo Puch\u00e1n", "initials": "DP"}, {"family": "Morice", "given": "Korentin", "initials": "K"}, {"family": "Gillet", "given": "Natacha", "initials": "N", "orcid": "0000-0002-7657-6861", "researcher": {"href": "https://publications.scilifelab.se/researcher/ce5b43ffd5e44fbe926a3d26064b6760.json"}}, {"family": "Ravindranath", "given": "Ranjitha", "initials": "R"}, {"family": "Patel", "given": "Ankit Kumar", "initials": "AK"}, {"family": "Sengupta", "given": "Pallabi", "initials": "P", "orcid": "0000-0002-1413-9412", "researcher": {"href": "https://publications.scilifelab.se/researcher/851c95f648f242e0ba67202279725796.json"}}, {"family": "Obi", "given": "Ikenna", "initials": "I", "orcid": "0000-0003-0364-8964", "researcher": {"href": "https://publications.scilifelab.se/researcher/e46c65e7b0e540b0a7c225d54c1502d7.json"}}, {"family": "Rodriguez-Marquez", "given": "Eva", "initials": "E"}, {"family": "Khrouz", "given": "Lhoussain", "initials": "L", "orcid": "0000-0001-7098-8534", "researcher": {"href": "https://publications.scilifelab.se/researcher/d0b94b97266943628d8e512a2f23c830.json"}}, {"family": "Dumont", "given": "Elise", "initials": "E", "orcid": "0000-0002-2359-111X", "researcher": {"href": "https://publications.scilifelab.se/researcher/9f7b8a4b687c4eacaa550a7b0e1824a0.json"}}, {"family": "Abad Gal\u00e1n", "given": "Laura", "initials": "L", "orcid": "0000-0001-6226-0850", "researcher": {"href": "https://publications.scilifelab.se/researcher/a9c02dcda84044c899f82b88313f5213.json"}}, {"family": "Allain", "given": "Magali", "initials": "M", "orcid": "0000-0002-0893-6028", "researcher": {"href": "https://publications.scilifelab.se/researcher/c9ae4c3cfe4348d5af5dc69a2bb365b0.json"}}, {"family": "Walker", "given": "Bright", "initials": "B", "orcid": "0000-0002-0613-8866", "researcher": {"href": "https://publications.scilifelab.se/researcher/0f28f9f1a7724dac86ba59c897d1ed48.json"}}, {"family": "Ahn", "given": "Hyun Seo", "initials": "HS", "orcid": "0000-0002-6014-0916", "researcher": {"href": "https://publications.scilifelab.se/researcher/45122ada8d3746e2acebcf805bf16e97.json"}}, {"family": "Maury", "given": "Olivier", "initials": "O", "orcid": "0000-0002-4639-643X", "researcher": {"href": "https://publications.scilifelab.se/researcher/bd39f301c4934cf7a3e61b7ba984e63a.json"}}, {"family": "Blanchard", "given": "Philippe", "initials": "P", "orcid": "0000-0002-9408-8108", "researcher": {"href": "https://publications.scilifelab.se/researcher/7b181cd702d54796a6e0058b5facf800.json"}}, {"family": "Le Bahers", "given": "Tangui", "initials": "T", "orcid": "0000-0003-2166-081X", "researcher": {"href": "https://publications.scilifelab.se/researcher/cda268d05eb24dc180332aa1bf3871be.json"}}, {"family": "\u00d6hlund", "given": "Daniel", "initials": "D", "orcid": "0000-0002-5847-2778", "researcher": {"href": "https://publications.scilifelab.se/researcher/42e9e473f68c460098a37e22d0a41369.json"}}, {"family": "von Hofsten", "given": "Jonas", "initials": "J", "orcid": "0000-0003-3730-1790", "researcher": {"href": "https://publications.scilifelab.se/researcher/ee9e4dc7b9a44b818e4c10aea633a934.json"}}, {"family": "Monnereau", "given": "Cyrille", "initials": "C", "orcid": "0000-0002-8928-2416", "researcher": {"href": "https://publications.scilifelab.se/researcher/1712a32086094d52abcac1252d4fd913.json"}}, {"family": "Cabanetos", "given": "Cl\u00e9ment", "initials": "C", "orcid": "0000-0003-3781-887X", "researcher": {"href": "https://publications.scilifelab.se/researcher/a03a47d2558643b4b1bbfd80286e9575.json"}}, {"family": "Sabouri", "given": "Nasim", "initials": "N", "orcid": "0000-0002-4541-7702", "researcher": {"href": "https://publications.scilifelab.se/researcher/4bdc688dc85a4932acfdfffad8bfc443.json"}}], "type": "journal article", "published": "2023-07-07", "journal": {"title": "Nucleic Acids Res.", "issn": "1362-4962", "volume": "51", "issue": "12", "pages": "6264-6285", "issn-l": "0305-1048"}, "abstract": "Photodynamic therapy (PDT) ideally relies on the administration, selective accumulation and photoactivation of a photosensitizer (PS) into diseased tissues. In this context, we report a new heavy-atom-free fluorescent G-quadruplex (G4) DNA-binding PS, named DBI. We reveal by fluorescence microscopy that DBI preferentially localizes in intraluminal vesicles (ILVs), precursors of exosomes, which are key components of cancer cell proliferation. Moreover, purified exosomal DNA was recognized by a G4-specific antibody, thus highlighting the presence of such G4-forming sequences in the vesicles. Despite the absence of fluorescence signal from DBI in nuclei, light-irradiated DBI-treated cells generated reactive oxygen species (ROS), triggering a 3-fold increase of nuclear G4 foci, slowing fork progression and elevated levels of both DNA base damage, 8-oxoguanine, and double-stranded DNA breaks. Consequently, DBI was found to exert significant phototoxic effects (at nanomolar scale) toward cancer cell lines and tumor organoids. Furthermore, in vivo testing reveals that photoactivation of DBI induces not only G4 formation and DNA damage but also apoptosis in zebrafish, specifically in the area where DBI had accumulated. Collectively, this approach shows significant promise for image-guided PDT.", "doi": "10.1093/nar/gkad365", "pmid": "37191066", "labels": {"Swedish NMR Centre": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC10325911"}, {"db": "pii", "key": "7160217"}], "notes": [], "created": "2024-01-18T13:01:00.383Z", "modified": "2025-10-17T13:03:53.699Z"}]}