{"entity": "researcher", "timestamp": "2026-08-13T17:42:55.685Z", "family": "Paslawski", "given": "Wojciech", "initials": "W", "orcid": "0000-0003-2141-4547", "affiliations": ["Department of Clinical Neuroscience, Neuro Svenningsson, Karolinska Institute, 171 76 Stockholm, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/ce039c7e5e774d66a8c0331a3c990ccc.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/ce039c7e5e774d66a8c0331a3c990ccc"}}, "publications": [{"entity": "publication", "iuid": "2110c851b57d4479b99eb959647a15e4", "links": {"self": {"href": "https://publications.scilifelab.se/publication/2110c851b57d4479b99eb959647a15e4.json"}, "display": {"href": "https://publications.scilifelab.se/publication/2110c851b57d4479b99eb959647a15e4"}}, "title": "High Cerebrospinal DOPA Decarboxylase Level Predicts Cognitive Decline in Parkinson's Disease.", "authors": [{"family": "Sturchio", "given": "Andrea", "initials": "A"}, {"family": "Paslawski", "given": "Wojciech", "initials": "W", "orcid": "0000-0003-2141-4547", "researcher": {"href": "https://publications.scilifelab.se/researcher/ce039c7e5e774d66a8c0331a3c990ccc.json"}}, {"family": "Khosousi", "given": "Shervin", "initials": "S"}, {"family": "Markaki", "given": "Ioanna", "initials": "I", "orcid": "0000-0003-3328-5746", "researcher": {"href": "https://publications.scilifelab.se/researcher/929b8fea137e410b96d1a6754690c447.json"}}, {"family": "Nalls", "given": "Michael A", "initials": "MA"}, {"family": "Singleton", "given": "Andrew B", "initials": "AB"}, {"family": "Iwaki", "given": "Hirotaka", "initials": "H"}, {"family": "Svenningsson", "given": "Per", "initials": "P", "orcid": "0000-0001-6727-3802", "researcher": {"href": "https://publications.scilifelab.se/researcher/5199496295334771ba3c5621a83a6f43.json"}}], "type": "journal article", "published": "2025-09-24", "journal": {"title": "Mov Disord Clin Pract", "issn": "2330-1619", "issn-l": null}, "abstract": "DOPA decarboxylase (DDC) in cerebrospinal fluid (CSF) is an emerging Parkinson's disease (PD) biomarker, but its association with nonmotor symptoms is unclear.\n\nWe aimed to determine if baseline DDC was associated with future cognitive decline in PD.\n\nWe correlated baseline CSF DDC, detected using the proximity extension assay, with Montreal Cognitive Assessment (MoCA) score using longitudinal data from 3 cohorts: Biopark, PPMI, and PDBP.\n\nDDC was significantly associated with cognitive decline in both the Biopark cohort (P-value < 0.0001) and the PDBP/PPMI cohorts (P-value < 0.0001). The results were still significant after correcting for levodopa-equivalent daily dose in the Biopark cohort (P-value < 0.0001) and when the analysis was restricted to the de novo subjects, both in Biopark (P-value: 0.0065) and PPMI (P-value<0.0001) cohorts.\n\nCSF DDC is a potential biomarker for the prediction of cognitive decline in PD patients.", "doi": "10.1002/mdc3.70367", "pmid": "40990085", "labels": {"Affinity Proteomics Stockholm": "Service"}, "xrefs": [], "notes": [], "created": "2025-11-24T22:25:28.475Z", "modified": "2025-11-24T22:25:28.974Z"}, {"entity": "publication", "iuid": "a3bdea4aa2d845f58b75e41eb55c7da1", "links": {"self": {"href": "https://publications.scilifelab.se/publication/a3bdea4aa2d845f58b75e41eb55c7da1.json"}, "display": {"href": "https://publications.scilifelab.se/publication/a3bdea4aa2d845f58b75e41eb55c7da1"}}, "title": "Increased CSF DOPA Decarboxylase Correlates with Lower DaT-SPECT Binding: Analyses in Biopark and PPMI Cohorts.", "authors": [{"family": "Khosousi", "given": "Shervin", "initials": "S"}, {"family": "Sturchio", "given": "Andrea", "initials": "A"}, {"family": "Appleton", "given": "Ellen", "initials": "E"}, {"family": "Paslawski", "given": "Wojciech", "initials": "W", "orcid": "0000-0003-2141-4547", "researcher": {"href": "https://publications.scilifelab.se/researcher/ce039c7e5e774d66a8c0331a3c990ccc.json"}}, {"family": "Ta", "given": "Michael", "initials": "M", "orcid": "0000-0001-6148-3841", "researcher": {"href": "https://publications.scilifelab.se/researcher/30644bee13c940409e1bca9a26bcfece.json"}}, {"family": "Nalls", "given": "Michael", "initials": "M"}, {"family": "Singleton", "given": "Andrew B", "initials": "AB"}, {"family": "Iwaki", "given": "Hirotaka", "initials": "H", "orcid": "0000-0002-8982-7885", "researcher": {"href": "https://publications.scilifelab.se/researcher/a35d8047c1ef434ab616cc370979d7e8.json"}}, {"family": "Svenningsson", "given": "Per", "initials": "P", "orcid": "0000-0001-6727-3802", "researcher": {"href": "https://publications.scilifelab.se/researcher/5199496295334771ba3c5621a83a6f43.json"}}], "type": "journal article", "published": "2024-10-00", "journal": {"title": "Mov Disord", "issn": "1531-8257", "volume": "39", "issue": "10", "pages": "1881-1885", "issn-l": null}, "abstract": "Recent studies identified increased cerebrospinal fluid (CSF) DOPA decarboxylase (DDC) as a promising biomarker for parkinsonian disorders, suggesting a compensation to dying dopaminergic neurons. A correlation with 123I-FP-CIT-SPECT (DaT-SPECT) imaging could shed light on this link.\n\nThe objective is to assess the relationship between CSF DDC levels and DaT-SPECT binding values.\n\nA total of 51 and 72 Parkinson's disease (PD) subjects with available DaT-SPECT and CSF DDC levels were selected from the PPMI and Biopark cohorts, respectively. DDC levels were analyzed using proximity extension assay and correlated with DaT-SPECT striatal binding ratios (SBR). All analyses were corrected for age and sex.\n\nCSF DDC levels in PD patients correlated negatively with DaT-SPECT SBR in both putamen and caudate nucleus. Additionally, SBR decreased with increased DDC levels over time in PD patients.\n\nCSF DDC levels negatively correlate with DaT-SPECT SBR in levodopa-treated PD. \u00a9 2024 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society.", "doi": "10.1002/mds.29835", "pmid": "38798037", "labels": {"Affinity Proteomics Stockholm": "Service"}, "xrefs": [{"db": "mid", "key": "NIHMS1991855"}, {"db": "pmc", "key": "PMC11490393"}], "notes": [], "created": "2024-11-14T23:01:21.209Z", "modified": "2024-11-14T23:01:24.058Z"}, {"entity": "publication", "iuid": "b940b2fe317c4dc4a3d7456dcd8cf7e8", "links": {"self": {"href": "https://publications.scilifelab.se/publication/b940b2fe317c4dc4a3d7456dcd8cf7e8.json"}, "display": {"href": "https://publications.scilifelab.se/publication/b940b2fe317c4dc4a3d7456dcd8cf7e8"}}, "title": "Secretome Analyses Identify FKBP4 as a GBA1-Associated Protein in CSF and iPS Cells from Parkinson's Disease Patients with GBA1 Mutations.", "authors": [{"family": "Kojima", "given": "Rika", "initials": "R", "orcid": "0000-0002-2745-960X", "researcher": {"href": "https://publications.scilifelab.se/researcher/f78bd62e8f3448cf995c39f0030451ad.json"}}, {"family": "Paslawski", "given": "Wojciech", "initials": "W", "orcid": "0000-0003-2141-4547", "researcher": {"href": "https://publications.scilifelab.se/researcher/ce039c7e5e774d66a8c0331a3c990ccc.json"}}, {"family": "Lyu", "given": "Guochang", "initials": "G"}, {"family": "Arenas", "given": "Ernest", "initials": "E", "orcid": "0000-0003-0197-6577", "researcher": {"href": "https://publications.scilifelab.se/researcher/a3bb18ad1c4b4dae99b60ab0ae13e36a.json"}}, {"family": "Zhang", "given": "Xiaoqun", "initials": "X"}, {"family": "Svenningsson", "given": "Per", "initials": "P", "orcid": "0000-0001-6727-3802", "researcher": {"href": "https://publications.scilifelab.se/researcher/5199496295334771ba3c5621a83a6f43.json"}}], "type": "journal article", "published": "2024-01-04", "journal": {"title": "Int J Mol Sci", "issn": "1422-0067", "volume": "25", "issue": "1", "issn-l": null}, "abstract": "Mutations in the GBA1 gene increase the risk of developing Parkinson's disease (PD). However, most carriers of GBA1 mutations do not develop PD throughout their lives. The mechanisms of how GBA1 mutations contribute to PD pathogenesis remain unclear. Cerebrospinal fluid (CSF) is used for detecting pathological conditions of diseases, providing insights into the molecular mechanisms underlying neurodegenerative disorders. In this study, we utilized the proximity extension assay to examine the levels of metabolism-linked protein in the CSF from 17 PD patients carrying GBA1 mutations (GBA1-PD) and 17 idiopathic PD (iPD). The analysis of CSF secretome in GBA1-PD identified 11 significantly altered proteins, namely FKBP4, THOP1, GLRX, TXNDC5, GAL, SEMA3F, CRKL, APLP1, LRP11, CD164, and NPTXR. To investigate GBA1-associated CSF changes attributed to specific neuronal subtypes responsible for PD, we analyzed the cell culture supernatant from GBA1-PD-induced pluripotent stem cell (iPSC)-derived midbrain dopaminergic (mDA) neurons. The secretome analysis of GBA1-PD iPSC-derived mDA neurons revealed that five differently regulated proteins overlapped with those identified in the CSF analysis: FKBP4, THOP1, GLRX, GAL, and CRKL. Reduced intracellular level of the top hit, FKPB4, was confirmed via Western Blot. In conclusion, our findings identify significantly altered CSF GBA1-PD-associated proteins with FKPB4 being firmly attributed to mDA neurons.", "doi": "10.3390/ijms25010683", "pmid": "38203854", "labels": {"Affinity Proteomics Stockholm": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC10779269"}, {"db": "pii", "key": "ijms25010683"}], "notes": [], "created": "2024-11-05T17:45:27.948Z", "modified": "2024-11-14T23:12:26.939Z"}, {"entity": "publication", "iuid": "ae4ff1411a31429f8d1c53a6fc16ed11", "links": {"self": {"href": "https://publications.scilifelab.se/publication/ae4ff1411a31429f8d1c53a6fc16ed11.json"}, "display": {"href": "https://publications.scilifelab.se/publication/ae4ff1411a31429f8d1c53a6fc16ed11"}}, "title": "PRC2-mediated repression is essential to maintain identity and function of differentiated dopaminergic and serotonergic neurons.", "authors": [{"family": "Toskas", "given": "Konstantinos", "initials": "K", "orcid": "0000-0002-5897-9501", "researcher": {"href": "https://publications.scilifelab.se/researcher/687adc14acf7400eaa552f41eb39807e.json"}}, {"family": "Yaghmaeian-Salmani", "given": "Behzad", "initials": "B", "orcid": "0000-0002-4221-6243", "researcher": {"href": "https://publications.scilifelab.se/researcher/eb9b5976d0b34622aff0e9a564b3ae54.json"}}, {"family": "Skiteva", "given": "Olga", "initials": "O"}, {"family": "Paslawski", "given": "Wojciech", "initials": "W", "orcid": "0000-0003-2141-4547", "researcher": {"href": "https://publications.scilifelab.se/researcher/ce039c7e5e774d66a8c0331a3c990ccc.json"}}, {"family": "Gillberg", "given": "Linda", "initials": "L"}, {"family": "Skara", "given": "Vasiliki", "initials": "V", "orcid": "0000-0002-0017-6116", "researcher": {"href": "https://publications.scilifelab.se/researcher/e412dda73e2e40a380e10775f45fbd66.json"}}, {"family": "Antoniou", "given": "Irene", "initials": "I", "orcid": "0000-0002-3616-9049", "researcher": {"href": "https://publications.scilifelab.se/researcher/1a00a046dece4cae9afa12a5c7caa723.json"}}, {"family": "S\u00f6dersten", "given": "Erik", "initials": "E"}, {"family": "Svenningsson", "given": "Per", "initials": "P", "orcid": "0000-0001-6727-3802", "researcher": {"href": "https://publications.scilifelab.se/researcher/5199496295334771ba3c5621a83a6f43.json"}}, {"family": "Chergui", "given": "Karima", "initials": "K", "orcid": "0000-0001-5702-0422", "researcher": {"href": "https://publications.scilifelab.se/researcher/be439b7cafce4d919881a7b0ef7d6340.json"}}, {"family": "Ringn\u00e9r", "given": "Markus", "initials": "M", "orcid": "0000-0001-5469-8940", "researcher": {"href": "https://publications.scilifelab.se/researcher/c0308e6d9c534033b431d2552cfe2af6.json"}}, {"family": "Perlmann", "given": "Thomas", "initials": "T"}, {"family": "Holmberg", "given": "Johan", "initials": "J", "orcid": "0000-0002-3018-001X", "researcher": {"href": "https://publications.scilifelab.se/researcher/f84bece88a264d228d3770ca634b4a19.json"}}], "type": "journal article", "published": "2022-08-26", "journal": {"title": "Sci Adv", "issn": "2375-2548", "issn-l": "2375-2548", "volume": "8", "issue": "34", "pages": "eabo1543"}, "abstract": "How neurons can maintain cellular identity over an entire life span remains largely unknown. Here, we show that maintenance of identity in differentiated dopaminergic and serotonergic neurons is critically reliant on the Polycomb repressive complex 2 (PRC2). Deletion of the obligate PRC2 component, Eed, in these neurons resulted in global loss of H3K27me3, followed by a gradual activation of genes harboring both H3K27me3 and H3K9me3 modifications. Notably, H3K9me3 was lost at these PRC2 targets before gene activation. Neuronal survival was not compromised; instead, there was a reduction in subtype-specific gene expression and a progressive impairment of dopaminergic and serotonergic neuronal function, leading to behavioral deficits characteristic of Parkinson's disease and anxiety. Single-cell analysis revealed subtype-specific vulnerability to loss of PRC2 repression in dopamine neurons of the substantia nigra. Our study reveals that a PRC2-dependent nonpermissive chromatin state is essential to maintain the subtype identity and function of dopaminergic and serotonergic neurons.", "doi": "10.1126/sciadv.abo1543", "pmid": "36026451", "labels": {"Bioinformatics Long-term Support WABI": "Collaborative", "Bioinformatics Support, Infrastructure and Training": "Collaborative", "Bioinformatics Support for Computational Resources": "Service", "NGI Single cell": "Service", "NGI Stockholm (Genomics Production)": "Service", "National Genomics Infrastructure": "Service", "NGI Stockholm (Genomics Applications)": "Service", "Bioinformatics (NBIS)": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC9417181"}], "notes": [], "created": "2022-08-30T11:39:49.253Z", "modified": "2024-10-14T11:50:28.807Z"}, {"entity": "publication", "iuid": "157102ef9ecf4b99814d91e18651d904", "links": {"self": {"href": "https://publications.scilifelab.se/publication/157102ef9ecf4b99814d91e18651d904.json"}, "display": {"href": "https://publications.scilifelab.se/publication/157102ef9ecf4b99814d91e18651d904"}}, "title": "\u03b1-synuclein-lipoprotein interactions and elevated ApoE level in cerebrospinal fluid from Parkinson's disease patients.", "authors": [{"family": "Paslawski", "given": "Wojciech", "initials": "W", "orcid": "0000-0003-2141-4547", "researcher": {"href": "https://publications.scilifelab.se/researcher/ce039c7e5e774d66a8c0331a3c990ccc.json"}}, {"family": "Zareba-Paslawska", "given": "Justyna", "initials": "J"}, {"family": "Zhang", "given": "Xiaoqun", "initials": "X"}, {"family": "H\u00f6lzl", "given": "Katharina", "initials": "K"}, {"family": "Wadensten", "given": "Henrik", "initials": "H"}, {"family": "Shariatgorji", "given": "Mohammadreza", "initials": "M"}, {"family": "Janelidze", "given": "Shorena", "initials": "S"}, {"family": "Hansson", "given": "Oskar", "initials": "O"}, {"family": "Forsgren", "given": "Lars", "initials": "L"}, {"family": "Andr\u00e9n", "given": "Per E", "initials": "PE", "orcid": "0000-0002-4062-7743", "researcher": {"href": "https://publications.scilifelab.se/researcher/64f6381de42949db8d30b56b526f3e26.json"}}, {"family": "Svenningsson", "given": "Per", "initials": "P"}], "type": "journal article", "published": "2019-07-23", "journal": {"title": "Proc. Natl. Acad. Sci. U.S.A.", "issn": "1091-6490", "volume": "116", "issue": "30", "pages": "15226-15235", "issn-l": "0027-8424"}, "abstract": "The progressive accumulation, aggregation, and spread of \u03b1-synuclein (\u03b1SN) are common hallmarks of Parkinson's disease (PD) pathology. Moreover, numerous proteins interact with \u03b1SN species, influencing its toxicity in the brain. In the present study, we extended analyses of \u03b1SN-interacting proteins to cerebrospinal fluid (CSF). Using coimmunoprecipitation, followed by mass spectrometry, we found that \u03b1SN colocalize with apolipoproteins on lipoprotein vesicles. We confirmed these interactions using several methods, including the enrichment of lipoproteins with a recombinant \u03b1SN, and the subsequent uptake of prepared vesicles by human dopaminergic neuronal-like cells. Further, we report an increased level of ApoE in CSF from early PD patients compared with matched controls in 3 independent cohorts. Moreover, in contrast to controls, we observed the presence of ApoE-positive neuromelanin-containing dopaminergic neurons in substantia nigra of PD patients. In conclusion, the cooccurrence of \u03b1SN on lipoprotein vesicles, and their uptake by dopaminergic neurons along with an increase of ApoE in early PD, proposes a mechanism(s) for \u03b1SN spreading in the extracellular milieu of PD.", "doi": "10.1073/pnas.1821409116", "pmid": "31270237", "labels": {"Spatial Mass Spectrometry": "Collaborative"}, "xrefs": [{"db": "pii", "key": "1821409116"}, {"db": "pmc", "key": "PMC6660770"}], "notes": [], "created": "2020-01-24T09:22:48.092Z", "modified": "2021-12-03T11:54:54.016Z"}]}