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"Yang", "given": "Hong", "initials": "H", "orcid": "0009-0002-0414-2471", "researcher": {"href": "https://publications.scilifelab.se/researcher/b52bdf7cbd1745e3a4578f17322c83f1.json"}}, {"family": "Ye\u015filkaya", "given": "Umit Haluk", "initials": "UH"}, {"family": "Yuan", "given": "Meng", "initials": "M", "orcid": "0000-0002-9248-3294", "researcher": {"href": "https://publications.scilifelab.se/researcher/befeb12cfba142eb9dadf81497d6c419.json"}}, {"family": "Zeybel", "given": "Mujdat", "initials": "M", "orcid": "0000-0001-5440-4623", "researcher": {"href": "https://publications.scilifelab.se/researcher/0559b83985134a0a9283ea39601a95a7.json"}}, {"family": "Zhang", "given": "Cheng", "initials": "C", "orcid": "0000-0002-3721-8586", "researcher": {"href": "https://publications.scilifelab.se/researcher/d1b9559ac41749fa91c4025108947e13.json"}}, {"family": "Zhong", "given": "Wen", "initials": "W", "orcid": "0000-0002-7422-6104", "researcher": {"href": "https://publications.scilifelab.se/researcher/a82c3b7da3b8472392d39ca5f6d5bedb.json"}}, {"family": "Zwahlen", "given": "Martin", "initials": "M", "orcid": "0000-0002-0064-4776", "researcher": {"href": "https://publications.scilifelab.se/researcher/04fb4e913dfb47b9bee48531db50d64c.json"}}, {"family": "von Feilitzen", "given": "Kalle", "initials": "K", "orcid": "0000-0002-0257-7554", "researcher": {"href": "https://publications.scilifelab.se/researcher/9f1e309f8d9247458c59e2ecfbd0c079.json"}}, {"family": "Nilsson", "given": "Peter", "initials": "P", "orcid": "0000-0002-4657-8532", "researcher": {"href": "https://publications.scilifelab.se/researcher/799bcf1cf8cf451296f4535dd4ca9dc0.json"}}, {"family": "Edfors", "given": "Fredrik", "initials": "F", "orcid": "0000-0002-0017-7987", "researcher": {"href": "https://publications.scilifelab.se/researcher/3f0e8af0b9144bcd9fd566d316008a62.json"}}, {"family": "Uhl\u00e9n", "given": "Mathias", "initials": "M", "orcid": "0000-0002-4858-8056", "researcher": {"href": "https://publications.scilifelab.se/researcher/ff81da3cb0cf4262873b993a1b06798c.json"}}], "type": "journal article", "published": "2025-10-09", "journal": {"title": "Science", "issn": "1095-9203", "issn-l": "0036-8075", "volume": null, "issue": null, "pages": "eadx2678"}, "abstract": "The human blood proteome provides a holistic readout of health states through the assessment of thousands of circulating proteins. Here, we present a pan-disease resource to enable the study of diverse disease phenotypes within a harmonized proteomics dataset. By profiling protein concentrations across 59 diseases and healthy cohorts, we identified proteins associated with age, sex, and BMI, as well as disease-specific signatures. This study highlights shared and distinct protein patterns across conditions, demonstrating the power of a unified proteomics approach to uncover biological insights. The dataset, covering 8,262 individuals and up to 5,416 proteins, serves as an online resource for exploring disease-specific protein profiles and advancing precision medicine research.", "doi": "10.1126/science.adx2678", "pmid": "41066540", "labels": {"Autoimmunity and Serology Profiling": "Service", "National Genomics Infrastructure": "Collaborative", "NGI Proteomics": "Collaborative", "NGI Uppsala (SNP&SEQ Technology Platform)": "Collaborative", "Affinity Proteomics Uppsala": "Collaborative"}, "xrefs": [], "notes": [], "created": "2025-10-10T10:37:22.942Z", "modified": "2025-11-07T07:45:07.446Z"}, {"entity": "publication", "iuid": "bc60a0502a14498fbdae1579b2effdc2", "links": {"self": {"href": "https://publications.scilifelab.se/publication/bc60a0502a14498fbdae1579b2effdc2.json"}, "display": {"href": "https://publications.scilifelab.se/publication/bc60a0502a14498fbdae1579b2effdc2"}}, "title": "Autoantibodies against a subunit of mitochondrial respiratory chain complex I in inclusion body myositis.", "authors": [{"family": "Notarnicola", "given": "Antonella", "initials": "A", "orcid": "0000-0003-0272-2931", "researcher": {"href": "https://publications.scilifelab.se/researcher/42411ecc60cd4357930ff0e978b3fcd8.json"}}, {"family": "Hellstrom", "given": "Ceke", "initials": "C", "orcid": "0000-0003-0880-5375", "researcher": {"href": "https://publications.scilifelab.se/researcher/dbf3f75938f0442a9b1ae5c98565f44a.json"}}, {"family": "Horuluoglu", "given": "Begum", "initials": "B"}, {"family": "Pin", "given": "Elisa", "initials": "E", "orcid": "0000-0002-2158-2674", "researcher": {"href": "https://publications.scilifelab.se/researcher/ccb4db02b9784587b62020716ab87247.json"}}, {"family": "Preger", "given": "Charlotta", "initials": "C", "orcid": "0000-0002-1342-9135", "researcher": {"href": "https://publications.scilifelab.se/researcher/d8519a1cc0e845c68b59d6ecdfea1a33.json"}}, {"family": "Bonomi", "given": "Francesco", "initials": "F"}, {"family": "De Paepe", "given": "Boel", "initials": "B"}, {"family": "De Bleecker", "given": "Jan L", "initials": "JL"}, {"family": "Van der Kooi", "given": "Anneke J", "initials": "AJ"}, {"family": "De Visser", "given": "Marianne", "initials": "M"}, {"family": "Sacconi", "given": "Sabrina", "initials": "S"}, {"family": "Machado", "given": "Pedro", "initials": "P"}, {"family": "Badrising", "given": "Umesh A", "initials": "UA"}, {"family": "Rietveld", "given": "Anke", "initials": "A"}, {"family": "Pruijn", "given": "Ger", "initials": "G"}, {"family": "Rothwell", "given": "Simon", "initials": "S", "orcid": "0000-0003-2123-9902", "researcher": {"href": "https://publications.scilifelab.se/researcher/b39ce853a3d041b68e4d4e82e64e3703.json"}}, {"family": "Lilleker", "given": "James B", "initials": "JB"}, {"family": "Chinoy", "given": "Hector", "initials": "H", "orcid": "0000-0001-6492-1288", "researcher": {"href": "https://publications.scilifelab.se/researcher/03b0a1d459104296baba3dedebf10e08.json"}}, {"family": "Benveniste", "given": "Olivier", "initials": "O"}, {"family": "Svenungsson", "given": "Elisabet", "initials": "E", "orcid": "0000-0003-3396-3244", "researcher": {"href": "https://publications.scilifelab.se/researcher/0ab5989c3c604a96bf42b1b6f90434a0.json"}}, {"family": "Idborg", "given": "Helena", "initials": "H", "orcid": "0000-0003-4041-4729", "researcher": {"href": "https://publications.scilifelab.se/researcher/eb0d55d31d77413abaa72e76c0472dc1.json"}}, {"family": "Jakobsson", "given": "Per-Johan", "initials": "P", "orcid": "0000-0001-7665-9715", "researcher": {"href": "https://publications.scilifelab.se/researcher/8fb148e2233b4541bc7c8b340f0cf12c.json"}}, {"family": "Nilsson", "given": "Peter", "initials": "P"}, {"family": "Lundberg", "given": "Ingrid E", "initials": "IE", "orcid": "0000-0002-6068-9212", "researcher": {"href": "https://publications.scilifelab.se/researcher/40f6c8e761a944b78e67f0e04453f78b.json"}}], "type": "journal article", "published": "2024-11-18", "journal": {"title": "J. Autoimmun.", "issn": "1095-9157", "issn-l": "0896-8411", "volume": "149", "issue": null, "pages": "103332"}, "abstract": "Autoantibodies are found in up to 80 % of patients with idiopathic inflammatory myopathies (IIM) and are associated with distinct clinical phenotypes. Autoantibodies targeting cytosolic 5'-nucleotidase 1A (anti-NT5C1A) are currently the only known serum biomarker for the subgroup inclusion body myositis (IBM), although detected even in other autoimmune diseases. The aim of the study was to identify new autoimmune targets in IIM.\r\n\r\nIn a first cross-sectional exploratory study, samples from 219 IIM (108 Polymyositis (PM), 80 Dermatomyositis (DM) and 31 IBM) patients, 349 Systemic Lupus Erythematosus (SLE) patients and 306 population controls were screened for IgG reactivity against a panel of 357 proteins using an antigen bead array. All samples were identified in the local biobank of the Rheumatology clinic, Karolinska University Hospital. Positive hits for the IBM subgroup were then validated in an independent larger cohort of 287 patients with IBM followed at nine European rheumatological or neurological centers. IBM serum samples were explored by antigen bead array and results validated by Western blot. As controls, sera from 29 patients with PM and 30 with DM, HLA-matched with the Swedish IBM cohort, were included. Demographics, laboratory, clinical, and muscle biopsy data of the IBM cohort was retrieved.\r\n\r\nIn the exploratory study, IgG reactivity towards NADH dehydrogenase 1 \u03b1 subcomplex 11 (NDUFA11), a subunit of the membrane-bound mitochondrial respiratory chain complex I, was discovered with higher frequency in the IBM (9.7 %) than PM (2.8 %) and DM samples (1.3 %), although the difference was not statistically significant. Anti-NDUFA11 IgG was also found in 1.4 % of SLE and 2.0 % of population control samples. In the validation study, anti-NDUFA11 autoantibodies were detected in 10/287 IBM patients (3.5 %), 0/29 p.m. and 0/30 DM patients. Reactivity against NDUFA11 could be confirmed by Western blot. No statistically significant differences were found between patients with and without anti-NDUFA11 antibodies when comparing clinical, laboratory and histological data. However, we observed a trend of higher frequency of distal lower extremity muscle weakness, ragged red fibers and higher CK levels at time of diagnosis in the anti-NDUFA11 positive group. Co-existence of anti-NDUFA11 and anti-NT5C1A antibodies was not observed in any IBM patient.\r\n\r\nOur results reveal a new autoimmune target in the mitochondrial respiratory chain complex I that might be specifically associated with IBM. This is of particular interest as mitochondrial abnormalities are known histological findings in muscle biopsies of IBM patients.", "doi": "10.1016/j.jaut.2024.103332", "pmid": "39561568", "labels": {"Autoimmunity and Serology Profiling": "Collaborative"}, "xrefs": [{"db": "pii", "key": "S0896-8411(24)00166-5"}], "notes": [], "created": "2024-11-25T12:19:45.739Z", "modified": "2025-04-07T09:26:23.573Z"}, {"entity": "publication", "iuid": "2ba158e4da984af6a8835a79e148a316", "links": {"self": {"href": "https://publications.scilifelab.se/publication/2ba158e4da984af6a8835a79e148a316.json"}, "display": {"href": "https://publications.scilifelab.se/publication/2ba158e4da984af6a8835a79e148a316"}}, "title": "Prevalent and persistent new-onset autoantibodies in mild to severe COVID-19.", "authors": [{"family": "Jernbom", "given": "August F", "initials": "AF", "orcid": "0000-0002-7773-1851", "researcher": {"href": "https://publications.scilifelab.se/researcher/fe6400bde68b46899515cef5bea05fca.json"}}, {"family": "Skoglund", "given": "Lovisa", "initials": "L", "orcid": "0009-0002-7863-1972", "researcher": {"href": "https://publications.scilifelab.se/researcher/e6bab6ea5e824b13ad5b77a6fa08523e.json"}}, {"family": "Pin", "given": "Elisa", "initials": "E", "orcid": "0000-0002-2158-2674", "researcher": {"href": "https://publications.scilifelab.se/researcher/ccb4db02b9784587b62020716ab87247.json"}}, {"family": "Sj\u00f6berg", "given": "Ronald", "initials": "R", "orcid": "0000-0003-1363-5796", "researcher": {"href": "https://publications.scilifelab.se/researcher/d08326da26da422ab445a26563843e79.json"}}, {"family": "Tegel", "given": "Hanna", "initials": "H", "orcid": "0000-0002-7067-9173", "researcher": {"href": "https://publications.scilifelab.se/researcher/d3d733dbd7b84a6b88f7f5fcff7165f6.json"}}, {"family": "Hober", "given": "Sophia", "initials": "S", "orcid": "0000-0003-0605-8417", "researcher": {"href": "https://publications.scilifelab.se/researcher/f8dd8ee4264d4e4b912dacad3106f40a.json"}}, {"family": "Rostami", "given": "Elham", "initials": "E", "orcid": "0000-0003-1218-6247", "researcher": {"href": "https://publications.scilifelab.se/researcher/a4e5d32d3023425c9940f1eb1ed876b4.json"}}, {"family": "Rasmusson", "given": "Annica", "initials": "A", "orcid": "0000-0002-7228-7755", "researcher": {"href": "https://publications.scilifelab.se/researcher/7ef833f6dd204c189586fba5c33d1d58.json"}}, {"family": "Cunningham", "given": "Janet L", "initials": "JL", "orcid": "0000-0001-7876-7779", "researcher": {"href": "https://publications.scilifelab.se/researcher/3ab30dd6c6874bc7a227a8699c4a7085.json"}}, {"family": "Havervall", "given": "Sebastian", "initials": "S"}, {"family": "Th\u00e5lin", "given": "Charlotte", "initials": "C", "orcid": "0000-0002-1345-6491", "researcher": {"href": "https://publications.scilifelab.se/researcher/130fb6ef6b774613a767e98f9f9b2eb4.json"}}, {"family": "M\u00e5nberg", "given": "Anna", "initials": "A", "orcid": "0000-0002-0056-1313", "researcher": {"href": "https://publications.scilifelab.se/researcher/6d155273b5b54e61b773f263e4f2ce9b.json"}}, {"family": "Nilsson", "given": "Peter", "initials": "P", "orcid": "0000-0002-4657-8532", "researcher": {"href": "https://publications.scilifelab.se/researcher/799bcf1cf8cf451296f4535dd4ca9dc0.json"}}], "type": "journal article", "published": "2024-10-17", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "15", "issue": "1", "pages": "8941", "issn-l": "2041-1723"}, "abstract": "Autoantibodies have been shown to be implied in COVID-19 but the emerging autoantibody repertoire remains largely unexplored. We investigated the new-onset autoantibody repertoire in 525 healthcare workers and hospitalized COVID-19 patients at five time points over a 16-month period in 2020 and 2021 using proteome-wide and targeted protein and peptide arrays. Our results show that prevalent new-onset autoantibodies against a wide range of antigens emerged following SARS-CoV-2 infection in relation to pre-infectious baseline samples and remained elevated for at least 12 months. We found an increased prevalence of new-onset autoantibodies after severe COVID-19 and demonstrated associations between distinct new-onset autoantibodies and neuropsychiatric symptoms post-COVID-19. Using epitope mapping, we determined the main epitopes of selected new-onset autoantibodies, validated them in independent cohorts of neuro-COVID and pre-pandemic healthy controls, and identified sequence similarities suggestive of molecular mimicry between main epitopes and the conserved fusion peptide of the SARS-CoV-2 Spike glycoprotein. Our work describes the complexity and dynamics of the autoantibody repertoire emerging with COVID-19 and supports the need for continued analysis of the new-onset autoantibody repertoire to elucidate the mechanisms of the post-COVID-19 condition.", "doi": "10.1038/s41467-024-53356-5", "pmid": "39414823", "labels": {"Autoimmunity and Serology Profiling": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC11484904"}, {"db": "pii", "key": "10.1038/s41467-024-53356-5"}], "notes": [], "created": "2024-10-21T13:07:04.870Z", "modified": "2024-10-21T13:07:05.227Z"}, {"entity": "publication", "iuid": "184914c5984c485498ee2d2068a27342", "links": {"self": {"href": "https://publications.scilifelab.se/publication/184914c5984c485498ee2d2068a27342.json"}, "display": {"href": "https://publications.scilifelab.se/publication/184914c5984c485498ee2d2068a27342"}}, "title": "Autoantibodies towards HFE and SYT5 in anti-neutrophil cytoplasm antibody-associated vasculitis relapse.", "authors": [{"family": "Bayati", "given": "Shaghayegh", "initials": "S"}, {"family": "Nazeer", "given": "Jamsheela", "initials": "J"}, {"family": "Ng", "given": "James", "initials": "J"}, {"family": "George", "given": "Angel M", "initials": "AM"}, {"family": "Hayes", "given": "Michael", "initials": "M"}, {"family": "Little", "given": "Mark A", "initials": "MA"}, {"family": "Nilsson", "given": "Peter", "initials": "P"}, {"family": "Pin", "given": "Elisa", "initials": "E", "orcid": "0000-0002-2158-2674", "researcher": {"href": "https://publications.scilifelab.se/researcher/ccb4db02b9784587b62020716ab87247.json"}}], "type": "journal article", "published": "2024-10-14", "journal": {"title": "Rheumatology (Oxford)", "issn": "1462-0332", "issn-l": "1462-0324", "volume": null, "issue": null, "pages": null}, "abstract": "Identification of those at high and low risk of disease relapse is a major unmet need in the management of patients with ANCA-associated vasculitis (AAV). Precise stratification would allow tailoring of immunosuppressive medication. We profiled the autoantibody repertoire of AAV patients in remission to identify novel autoantibodies associated with relapse risk.\r\n\r\nPlasma samples collected from 246 AAV patients in remission were screened for novel autoantibodies using in-house generated protein arrays including 42 000 protein fragments representing 18 000 unique human proteins. Patients were categorized based on the occurrence and frequency of relapses. We modelled the association between these antibodies and relapse occurrence using descriptive and high dimensional regression approaches.\r\n\r\nWe observed nine autoantibodies at higher frequency in samples from AAV patients experiencing multiple relapses compared with patients in long-term remission off therapy (LTROT). LASSO analysis identified six autoantibodies that exhibited an association with relapse occurrence after sample collection. Antibodies targeting HFE and SYT5 were identified as associated with relapse in both analyses.\r\n\r\nThrough a broad protein array-based autoantibody screening, we identified two novel autoantibodies directed against HFE and SYT5 as candidate biomarkers of relapse in AAV.", "doi": "10.1093/rheumatology/keae540", "pmid": "39400561", "labels": {"Autoimmunity and Serology Profiling": "Collaborative"}, "xrefs": [{"db": "pii", "key": "7821207"}], "notes": [], "created": "2024-11-25T12:18:00.839Z", "modified": "2024-11-25T12:19:11.720Z"}, {"entity": "publication", "iuid": "b256fd79deef4afa9342a5889196bf56", "links": {"self": {"href": "https://publications.scilifelab.se/publication/b256fd79deef4afa9342a5889196bf56.json"}, "display": {"href": "https://publications.scilifelab.se/publication/b256fd79deef4afa9342a5889196bf56"}}, "title": "Seroepidemiological assessment of the spread of SARS-CoV-2 among 25 and 28 year-old adult women in Finland between March 2020-June 2022.", "authors": [{"family": "Gray", "given": "Penelope", "initials": "P", "orcid": "0000-0001-9065-4734", "researcher": {"href": "https://publications.scilifelab.se/researcher/26b7c7b28e3a492f8a336203eef01ed9.json"}}, {"family": "Eriksson", "given": "Tiina", "initials": "T"}, {"family": "Skoglund", "given": "Lovisa", "initials": "L"}, {"family": "Lagheden", "given": "Camilla", "initials": "C"}, {"family": "Hellstr\u00f6m", "given": "Ceke", "initials": "C"}, {"family": "Pin", "given": "Elisa", "initials": "E", "orcid": "0000-0002-2158-2674", "researcher": {"href": "https://publications.scilifelab.se/researcher/ccb4db02b9784587b62020716ab87247.json"}}, {"family": "Suomenrinne-Nordvik", "given": "Anna", "initials": "A", "orcid": "0000-0001-7678-6554", "researcher": {"href": "https://publications.scilifelab.se/researcher/d8c2b8add04644429a01296f81e26d74.json"}}, {"family": "Pimenoff", "given": "Ville N", "initials": "VN", "orcid": "0000-0002-0813-7031", "researcher": {"href": "https://publications.scilifelab.se/researcher/96cbbc36d7214b94a798b4c63eeb6a3a.json"}}, {"family": "Nilsson", "given": "Peter", "initials": "P"}, {"family": "Dillner", "given": "Joakim", "initials": "J"}, {"family": "Lehtinen", "given": "Matti", "initials": "M"}], "type": "journal article", "published": "2024-07-11", "journal": {"title": "PLoS ONE", "issn": "1932-6203", "volume": "19", "issue": "7", "pages": "e0305285", "issn-l": "1932-6203"}, "abstract": "Serological surveys of the prevalence of SARS-CoV-2 are instrumental to understanding the course of the COVID-19 epidemic. We evaluate the seroprevalence of SARS-CoV-2 among young adult Finnish females residing in 25 communities all over Finland from 2020 until 2022.\n\nBetween 1st March 2020 and 30th June 2022, 3589 blood samples were collected from 3583 women born in 1992-95 when aged 25 or 28 years old attending the follow-up of an ongoing population-based trial of cervical screening strategies. The crude and population standardized SARS-CoV-2 seroprevalence was measured using nucleocapsid (induced by infection) and spike wild-type (WT) protein (induced both by infection and by vaccination) antigens over time and stratified by place of residence (inside or outside the Helsinki metropolitan region).\n\nDuring 2020 (before vaccinations), spike-WT and nucleocapsid IgG antibodies followed each other closely, at very low levels (<5%). Spike-WT seropositivity increased rapidly concomitant with mass vaccinations in 2021 and reached 96.3% in the 2nd quartile of 2022. Antibodies to nucleocapsid IgG remained relatively infrequent throughput 2020-2021, increasing rapidly in the 1st and 2nd quartiles of 2022 (to 19.7% and 56.6% respectively). The nucleocapsid IgG seropositivity increased more profoundly in participants residing in the Helsinki metropolitan region (4.5%, 8.4% and 43.9% in 2020, 2021 and 2022 respectively) compared to those residing in communities outside the capital region (4.5%, 4.3% and 34.7%).\n\nLow SARS-CoV-2 infection-related seroprevalence during 2020-2021 suggest a comparatively successful infection control. Antibodies to the SARS-CoV-2 WT spike protein became extremely common among young women by the end of 2021, in line with the high uptake of SARS-CoV-2 vaccination. Finally, the rapid increase of seroprevalences to the SARS-CoV-2 nucleocapsid protein during the first and second quartile of 2022, imply a high incidence of infections with SARS-CoV-2 variants able to escape vaccine-induced protection.", "doi": "10.1371/journal.pone.0305285", "pmid": "38990856", "labels": {"Autoimmunity and Serology Profiling": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC11238966"}, {"db": "pii", "key": "PONE-D-23-42685"}], "notes": [], "created": "2024-09-04T07:31:43.842Z", "modified": "2024-09-04T07:31:43.965Z"}, {"entity": "publication", "iuid": "cbfa77436f394919be69926d5f93ccec", "links": {"self": {"href": "https://publications.scilifelab.se/publication/cbfa77436f394919be69926d5f93ccec.json"}, "display": {"href": "https://publications.scilifelab.se/publication/cbfa77436f394919be69926d5f93ccec"}}, "title": "Autoantibody Profiling and Anti-Kinesin Reactivity in ANCA-Associated Vasculitis.", "authors": [{"family": "Mescia", "given": "Federica", "initials": "F", "orcid": "0000-0002-2759-4027", "researcher": {"href": "https://publications.scilifelab.se/researcher/7c89c9c9abb44c59be30e792aa4a11ac.json"}}, {"family": "Bayati", "given": "Shaghayegh", "initials": "S"}, {"family": "Brouwer", "given": "Elisabeth", "initials": "E"}, {"family": "Heeringa", "given": "Peter", "initials": "P", "orcid": "0000-0001-8684-763X", "researcher": {"href": "https://publications.scilifelab.se/researcher/121f655c044d4b3894c195e3ffc2092a.json"}}, {"family": "Toonen", "given": "Erik J M", "initials": "EJM", "orcid": "0000-0001-9039-635X", "researcher": {"href": "https://publications.scilifelab.se/researcher/1d6d9551815f451b90c317b53766414e.json"}}, {"family": "Beenes", "given": "Marijke", "initials": "M"}, {"family": "Ball", "given": "Miriam J", "initials": "MJ"}, {"family": "Rees", "given": "Andrew J", "initials": "AJ"}, {"family": "Kain", "given": "Renate", "initials": "R", "orcid": "0000-0002-2428-543X", "researcher": {"href": "https://publications.scilifelab.se/researcher/87df5c95b8df4918ac438036cd7f2c58.json"}}, {"family": "Lyons", "given": "Paul A", "initials": "PA", "orcid": "0000-0001-7035-8997", "researcher": {"href": "https://publications.scilifelab.se/researcher/d405b26b9a714585b9db292bafd4892f.json"}}, {"family": "Nilsson", "given": "Peter", "initials": "P", "orcid": "0000-0002-4657-8532", "researcher": {"href": "https://publications.scilifelab.se/researcher/799bcf1cf8cf451296f4535dd4ca9dc0.json"}}, {"family": "Pin", "given": "Elisa", "initials": "E", "orcid": "0000-0002-2158-2674", "researcher": {"href": "https://publications.scilifelab.se/researcher/ccb4db02b9784587b62020716ab87247.json"}}], "type": "journal article", "published": "2023-10-19", "journal": {"title": "Int J Mol Sci", "issn": "1422-0067", "volume": "24", "issue": "20", "issn-l": null}, "abstract": "ANCA-associated vasculitides (AAV) are rare autoimmune diseases causing inflammation and damage to small blood vessels. New autoantibody biomarkers are needed to improve the diagnosis and treatment of AAV patients. In this study, we aimed to profile the autoantibody repertoire of AAV patients using in-house developed antigen arrays to identify previously unreported antibodies linked to the disease per se, clinical subgroups, or clinical activity. A total of 1743 protein fragments representing 1561 unique proteins were screened in 229 serum samples collected from 137 AAV patients at presentation, remission, and relapse. Additionally, serum samples from healthy individuals and patients with other type of vasculitis and autoimmune-inflammatory conditions were included to evaluate the specificity of the autoantibodies identified in AAV. Autoreactivity against members of the kinesin protein family were identified in AAV patients, healthy volunteers, and disease controls. Anti-KIF4A antibodies were significantly more prevalent in AAV. We also observed possible associations between anti-kinesin antibodies and clinically relevant features within AAV patients. Further verification studies will be needed to confirm these findings.", "doi": "10.3390/ijms242015341", "pmid": "37895021", "labels": {"Autoimmunity and Serology Profiling": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC10607136"}, {"db": "pii", "key": "ijms242015341"}], "notes": [], "created": "2024-09-04T07:29:39.837Z", "modified": "2024-09-04T07:29:40.418Z"}, {"entity": "publication", "iuid": "1292ce8474234989b763b9a52fa7186c", "links": {"self": {"href": "https://publications.scilifelab.se/publication/1292ce8474234989b763b9a52fa7186c.json"}, "display": {"href": "https://publications.scilifelab.se/publication/1292ce8474234989b763b9a52fa7186c"}}, "title": "The impact of immunomodulating treatment on the immunogenicity of COVID-19 vaccines in patients with immune-mediated inflammatory rheumatic diseases compared to healthy controls. A Swedish nationwide study (COVID19-REUMA).", "authors": [{"family": "Frodlund", "given": "Martina", "initials": "M"}, {"family": "Nived", "given": "Per", "initials": "P", "orcid": "0000-0003-0879-8195", "researcher": {"href": "https://publications.scilifelab.se/researcher/b6fb7542dac447caa7cce3ae562d60ec.json"}}, {"family": "Chatzidionysiou", "given": "Aikaterini", "initials": "A", "orcid": "0000-0002-2669-1247", "researcher": {"href": "https://publications.scilifelab.se/researcher/6b9a88c2fbe94c3698e9681dff80fed5.json"}}, {"family": "S\u00f6dergren", "given": "Anna", "initials": "A", "orcid": "0000-0002-7436-7900", "researcher": {"href": "https://publications.scilifelab.se/researcher/18d220b832fb45889249c243b179540f.json"}}, {"family": "Klingberg", "given": "Eva", "initials": "E", "orcid": "0000-0001-6858-6413", "researcher": {"href": "https://publications.scilifelab.se/researcher/a0dbd5b2aa28413b97026db7b84ea412.json"}}, {"family": "Bengtsson", "given": "Anders", "initials": "A"}, {"family": "Hansson", "given": "Monika", "initials": "M"}, {"family": "Olsson", "given": "Sophie", "initials": "S"}, {"family": "Pin", "given": "Elisa", "initials": "E", "orcid": "0000-0002-2158-2674", "researcher": {"href": "https://publications.scilifelab.se/researcher/ccb4db02b9784587b62020716ab87247.json"}}, {"family": "Klareskog", "given": "Lars", "initials": "L"}, {"family": "Kapetanovic", "given": "Meliha C", "initials": "MC", "orcid": "0000-0002-7853-514X", "researcher": {"href": "https://publications.scilifelab.se/researcher/cb145f4fa71747749a8609a84fad5987.json"}}], "type": "journal article", "published": "2023-05-11", "journal": {"title": "Vaccine", "issn": "1873-2518", "issn-l": null, "volume": "41", "issue": "20", "pages": "3247-3257"}, "abstract": "To elucidate antibody responses after the second and third dose of COVID-19 vaccine in patients with inflammatory rheumatic diseases (IRD) treated with biologic/targeted disease modifying anti-rheumatic drugs (b/ts DMARDs).\n\nAntibody levels to antigens representing spike full length protein and spike S1 were measured before vaccination, 2-12 weeks after the second dose, before and after the third dose using multiplex bead-based serology assay. Positive antibody response was defined as antibody levels over cut off (seropositivity) in seronegative individuals or \u2265 4-fold increase in antibodies in individuals seropositive for both spike proteins.\n\nPatients (n = 414) receiving b/ts DMARDs (283 had arthritis, 75 systemic vasculitis and 56 other autoimmune diseases) and controls (n = 61) from five Swedish regions participated. Treatments groups were: rituximab (n = 145); abatacept (n = 22); Interleukin 6 receptor inhibitors [IL6i (n = 79)]; JAnus Kinase Inhibitors [JAKi (n = 58)], Tumour Necrosis Factor inhibitor [TNFi (n = 68)] and Interleukin12/23/17 inhibitors [IL12/23/17i (n = 42)]. Percentage of patients with positive antibody response after two doses was significantly lower in rituximab (33,8%) and abatacept (40,9%) (p < 0,001) but not in IL12/23/17i, TNFi or JAKi groups compared to controls (80,3%). Higher age, rituximab treatment and shorter time between last rituximab course and vaccination predicted impaired antibody response. Antibody levels collected 21-40 weeks after second dose decreased significantly (IL6i: p = 0,02; other groups: p < 0,001) compared to levels at 2-12 week but most participants remained seropositive. Proportion of patients with positive antibody response increased after third dose but was still significantly lower in rituximab (p < 0,001).\n\nOlder individuals and patients on maintenance rituximab have an impaired response after two doses of COVID-19 vaccine which improves if the time between last rituximab course and vaccination extends and also after an additional vaccine dose. Rituximab patients should be prioritized for booster vaccine doses. TNFi, JAKi and IL12/23/17i does not diminished humoral response to primary and an additional vaccination.", "doi": "10.1016/j.vaccine.2023.03.065", "pmid": "37076360", "labels": {"Autoimmunity and Serology Profiling": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC10070777"}, {"db": "pii", "key": "S0264-410X(23)00372-9"}], "notes": [], "created": "2023-04-08T19:24:09.107Z", "modified": "2023-06-19T13:39:51.077Z"}, {"entity": "publication", "iuid": "71a0bf37a08a4b598531df666d3e7a56", "links": {"self": {"href": "https://publications.scilifelab.se/publication/71a0bf37a08a4b598531df666d3e7a56.json"}, "display": {"href": "https://publications.scilifelab.se/publication/71a0bf37a08a4b598531df666d3e7a56"}}, "title": "Autoantibodies against PIP4K2B and AKT3 Are Associated with Skin and Lung Fibrosis in Patients with Systemic Sclerosis.", "authors": [{"family": "Geroldinger-Simi\u0107", "given": "Marija", "initials": "M", "orcid": "0000-0003-2789-1862", "researcher": {"href": "https://publications.scilifelab.se/researcher/e87141eb33c34783a5c051e604a087af.json"}}, {"family": "Bayati", "given": "Shaghayegh", "initials": "S", "orcid": "0000-0002-3745-4570", "researcher": {"href": "https://publications.scilifelab.se/researcher/219f304209674c4f9ed726cccb170998.json"}}, {"family": "Pohjanen", "given": "Emmie", "initials": "E"}, {"family": "Sepp", "given": "Norbert", "initials": "N"}, {"family": "Nilsson", "given": "Peter", "initials": "P"}, {"family": "Pin", "given": "Elisa", "initials": "E", "orcid": "0000-0002-2158-2674", "researcher": {"href": "https://publications.scilifelab.se/researcher/ccb4db02b9784587b62020716ab87247.json"}}], "type": "journal article", "published": "2023-03-15", "journal": {"title": "Int J Mol Sci", "issn": "1422-0067", "volume": "24", "issue": "6", "issn-l": null}, "abstract": "Systemic sclerosis (SSc) is a rare autoimmune systemic disease that leads to decreased survival and quality of life due to fibrosis, inflammation, and vascular damage in the skin and/or vital organs. Early diagnosis is crucial for clinical benefit in SSc patients. Our study aimed to identify autoantibodies in the plasma of SSc patients that are associated with fibrosis in SSc. Initially, we performed a proteome-wide screening on sample pools from SSc patients by untargeted autoantibody screening on a planar antigen array (including 42,000 antigens representing 18,000 unique proteins). The selection was complemented with proteins reported in the literature in the context of SSc. A targeted antigen bead array was then generated with protein fragments representing the selected proteins and used to screen 55 SSc plasma samples and 52 matched controls. We found eleven autoantibodies with a higher prevalence in SSc patients than in controls, eight of which bound to proteins associated with fibrosis. Combining these autoantibodies in a panel could lead to the subgrouping of SSc patients with fibrosis. Anti-Phosphatidylinositol-5-phosphate 4-kinase type 2 beta (PIP4K2B)- and anti-AKT Serine/Threonine Kinase 3 (AKT3)-antibodies should be further explored to confirm their association with skin and lung fibrosis in SSc patients.", "doi": "10.3390/ijms24065629", "pmid": "36982700", "labels": {"Autoimmunity and Serology Profiling": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC10051301"}, {"db": "pii", "key": "ijms24065629"}], "notes": [], "created": "2023-04-08T19:25:04.020Z", "modified": "2023-04-08T19:25:04.134Z"}, {"entity": "publication", "iuid": "6cc0ac07f09a4ceebc3c06cb9256e5cb", "links": {"self": {"href": "https://publications.scilifelab.se/publication/6cc0ac07f09a4ceebc3c06cb9256e5cb.json"}, "display": {"href": "https://publications.scilifelab.se/publication/6cc0ac07f09a4ceebc3c06cb9256e5cb"}}, "title": "Hybrid immunity in immunocompromised patients with CLL after SARS-CoV-2 infection followed by booster mRNA vaccination.", "authors": [{"family": "Lisa", "given": "Blixt", "initials": "B"}, {"family": "Gao", "given": "Yu", "initials": "Y", "orcid": "0000-0002-4615-3614", "researcher": {"href": "https://publications.scilifelab.se/researcher/3915993e75dc4151bfc8ccab93895a40.json"}}, {"family": "Wullimann", "given": "David", "initials": "D", "orcid": "0000-0003-1632-0394", "researcher": {"href": "https://publications.scilifelab.se/researcher/349d6f425343417a93a83093b8863391.json"}}, {"family": "Mur\u00e9n Ingelman-Sundberg", "given": "Hanna", "initials": "H"}, {"family": "Muschiol", "given": "Sandra", "initials": "S"}, {"family": "Healy", "given": "Katie", "initials": "K", "orcid": "0000-0002-7242-6604", "researcher": {"href": "https://publications.scilifelab.se/researcher/399f5a83e04a4929ae03f265d57da92a.json"}}, {"family": "Bogdanovic", "given": "Gordana", "initials": "G"}, {"family": "Pin", "given": "Elisa", "initials": "E", "orcid": "0000-0002-2158-2674", "researcher": {"href": "https://publications.scilifelab.se/researcher/ccb4db02b9784587b62020716ab87247.json"}}, {"family": "Nilsson", "given": "Peter", "initials": "P", "orcid": "0000-0002-4657-8532", "researcher": {"href": "https://publications.scilifelab.se/researcher/799bcf1cf8cf451296f4535dd4ca9dc0.json"}}, {"family": "Kjellander", "given": "Christian", "initials": "C"}, {"family": "Grifoni", "given": "Alba", "initials": "A"}, {"family": "Sette", "given": "Alessandro", "initials": "A"}, {"family": "S\u00e4llberg Chen", "given": "Margaret", "initials": "M", "orcid": "0000-0002-3793-4064", "researcher": {"href": "https://publications.scilifelab.se/researcher/af31e38e29a24d208df80184c563357f.json"}}, {"family": "Ljunggren", "given": "Hans-Gustaf", "initials": "H"}, {"family": "Buggert", "given": "Marcus", "initials": "M"}, {"family": "Hansson", "given": "Lotta", "initials": "L", "orcid": "0000-0001-6614-5737", "researcher": {"href": "https://publications.scilifelab.se/researcher/754f78f2a56143b4a3fe80294227f7ae.json"}}, {"family": "Osterborg", "given": "Anders", "initials": "A"}], "type": "journal article", "published": "2022-09-23", "journal": {"title": "Blood", "issn": "1528-0020", "issn-l": "0006-4971", "volume": null, "issue": null, "pages": null}, "abstract": null, "doi": "10.1182/blood.2022016815", "pmid": "36150168", "labels": {"Autoimmunity and Serology Profiling": "Collaborative"}, "xrefs": [{"db": "pii", "key": "486704"}, {"db": "pmc", "key": "PMC9512527"}], "notes": [], "created": "2022-10-02T20:55:03.352Z", "modified": "2023-06-27T07:07:12.345Z"}, {"entity": "publication", "iuid": "d05ad8156c2c4552aa167cc2f77c5f92", "links": {"self": {"href": "https://publications.scilifelab.se/publication/d05ad8156c2c4552aa167cc2f77c5f92.json"}, "display": {"href": "https://publications.scilifelab.se/publication/d05ad8156c2c4552aa167cc2f77c5f92"}}, "title": "Identification of driver genes for critical forms of COVID-19 in a deeply phenotyped young patient cohort", "authors": [{"family": "Carapito", "given": "Raphael", "initials": "R", "orcid": "0000-0002-7036-442X", "researcher": {"href": "https://publications.scilifelab.se/researcher/76903b22cdeb455e836b9653637e5863.json"}}, {"family": "Li", "given": "Richard", "initials": "R", "orcid": "0000-0003-0741-337X", "researcher": {"href": "https://publications.scilifelab.se/researcher/b6beb7b6fa854ea1894e2f4d73f960e6.json"}}, {"family": "Helms", "given": "Julie", "initials": "J"}, {"family": "Carapito", "given": "Christine", "initials": "C", "orcid": "0000-0002-0079-319X", "researcher": {"href": "https://publications.scilifelab.se/researcher/40c906d688c94e41946b96261474db92.json"}}, {"family": "Gujja", "given": "Sharvari", "initials": "S"}, {"family": "Rolli", "given": "V\u00e9ronique", "initials": "V", "orcid": "0000-0002-1215-9436", "researcher": {"href": "https://publications.scilifelab.se/researcher/7eaa0e60b3cb41a78f4d8dcb40e917cc.json"}}, {"family": "Guimaraes", "given": "Raony", "initials": "R"}, {"family": "Malagon-Lopez", "given": "Jose", "initials": "J"}, {"family": "Spinnhirny", "given": "Perrine", "initials": "P"}, {"family": "Lederle", "given": "Alexandre", "initials": "A"}, {"family": "Mohseninia", "given": "Razieh", "initials": "R"}, {"family": "Hirschler", "given": "Aur\u00e9lie", "initials": "A", "orcid": "0000-0001-5066-6263", "researcher": {"href": "https://publications.scilifelab.se/researcher/bd804c98aada4e44976c12dd2912c38d.json"}}, {"family": "Muller", "given": "Leslie", "initials": "L"}, {"family": "Bastard", "given": "Paul", "initials": "P", "orcid": "0000-0002-5926-8437", "researcher": {"href": "https://publications.scilifelab.se/researcher/3891533a59dc4185ba934b16e416e6ce.json"}}, {"family": "Gervais", "given": "Adrian", "initials": "A", "orcid": "0000-0002-1083-5787", "researcher": {"href": "https://publications.scilifelab.se/researcher/f4d47775a4da4ee6ac0ff7da16c0155c.json"}}, {"family": "Zhang", "given": "Qian", "initials": "Q", "orcid": "0000-0002-9040-3289", "researcher": {"href": "https://publications.scilifelab.se/researcher/5bee352280384ad99b180f4f4bac42f5.json"}}, {"family": "Danion", "given": "Fran\u00e7ois", "initials": "F", "orcid": "0000-0003-3907-0658", "researcher": {"href": "https://publications.scilifelab.se/researcher/010a92d1146e4dd484553305bf3d5c74.json"}}, {"family": "Ruch", "given": "Yvon", "initials": "Y", "orcid": "0000-0002-4713-9478", "researcher": {"href": "https://publications.scilifelab.se/researcher/866834d1c2554ce2aa0d9e3823aa4c10.json"}}, {"family": "Schenck", "given": "Maleka", "initials": "M", "orcid": "0000-0002-1043-6452", "researcher": {"href": "https://publications.scilifelab.se/researcher/cdf8cad93b2b492ab7c6a86f6fb8109d.json"}}, {"family": "Collange", "given": "Olivier", "initials": "O"}, {"family": "Chamaraux-Tran", "given": "Thi\u00ean Nga", "initials": "TN"}, {"family": "Molitor", "given": "Anne", "initials": "A", "orcid": "0000-0003-1646-2325", "researcher": {"href": "https://publications.scilifelab.se/researcher/f1348a6ee6524f8886c48fd1c2b73b28.json"}}, {"family": "Pichot", "given": "Ang\u00e9lique", "initials": "A", "orcid": "0000-0003-2515-5538", "researcher": {"href": "https://publications.scilifelab.se/researcher/4aacb269ddaf4f5a8f2209b8f547a6a9.json"}}, {"family": "Bernard", "given": "Alice", "initials": "A"}, {"family": "Tahar", "given": "Ouria", "initials": "O", "orcid": "0000-0002-0075-2590", "researcher": {"href": "https://publications.scilifelab.se/researcher/a804ab13daff43f3b6888cd27b766b91.json"}}, {"family": "Bibi-Triki", "given": "Sabrina", "initials": "S"}, {"family": "Wu", "given": "Haiguo", "initials": "H"}, {"family": "Paul", "given": "Nicod\u00e8me", "initials": "N", "orcid": "0000-0003-4680-3012", "researcher": {"href": "https://publications.scilifelab.se/researcher/a9d8924cb3264f119c43c97feb3257b3.json"}}, {"family": "Mayeur", "given": "Sylvain", "initials": "S"}, {"family": "Larnicol", "given": "Annabel", "initials": "A"}, {"family": "Laumond", "given": "G\u00e9raldine", "initials": "G"}, {"family": "Frappier", "given": "Julia", "initials": "J", "orcid": "0000-0003-2618-8817", "researcher": {"href": "https://publications.scilifelab.se/researcher/e3ece157db0949298e13cc370703b60f.json"}}, {"family": "Schmidt", "given": "Sylvie", "initials": "S"}, {"family": "Hanauer", "given": "Antoine", "initials": "A"}, {"family": "Macquin", "given": "C\u00e9cile", "initials": "C", "orcid": "0000-0003-3194-7080", "researcher": {"href": "https://publications.scilifelab.se/researcher/cbcf074bed9b46e0872d01c65a91cb76.json"}}, {"family": "Stemmelen", "given": "Tristan", "initials": "T", "orcid": "0000-0002-4382-1277", "researcher": {"href": "https://publications.scilifelab.se/researcher/a821c104c65e48dead6301338168a18b.json"}}, {"family": "Simons", "given": "Michael", "initials": "M", "orcid": "0000-0003-0348-7734", "researcher": {"href": "https://publications.scilifelab.se/researcher/19fa7012d92c41cda7c5757a2c50c5be.json"}}, {"family": "Mariette", "given": "Xavier", "initials": "X", "orcid": "0000-0002-4244-5417", "researcher": {"href": "https://publications.scilifelab.se/researcher/13855fe7b68b4235a48a8dda9a0d5fd6.json"}}, {"family": "Hermine", "given": "Olivier", "initials": "O", "orcid": "0000-0003-2574-3874", "researcher": {"href": "https://publications.scilifelab.se/researcher/ac1bbf2973ff4bababdb07cc23398dd9.json"}}, {"family": "Fafi-Kremer", "given": "Samira", "initials": "S", "orcid": "0000-0003-3886-7833", "researcher": {"href": "https://publications.scilifelab.se/researcher/eb83f475c4ad4c3c80df18c9d3451652.json"}}, {"family": "Goichot", "given": "Bernard", "initials": "B", "orcid": "0000-0001-7794-1683", "researcher": {"href": "https://publications.scilifelab.se/researcher/29d2670d923f4bbd99a441bbf425584c.json"}}, {"family": "Drenou", "given": "Bernard", "initials": "B", "orcid": "0000-0002-2175-6785", "researcher": {"href": "https://publications.scilifelab.se/researcher/8250de3cdb9a4fc3be54b7e3e8fd1eef.json"}}, {"family": "Kuteifan", "given": "Khaldoun", "initials": "K", "orcid": "0000-0002-0428-7016", "researcher": {"href": "https://publications.scilifelab.se/researcher/d8d76418273b4af1bf4e69d098f3007c.json"}}, {"family": "Pottecher", "given": "Julien", "initials": "J"}, {"family": "Mertes", "given": "Paul Michel", "initials": "PM", "orcid": "0000-0002-6060-9438", "researcher": {"href": "https://publications.scilifelab.se/researcher/f147972b91414f6b87477b0859897927.json"}}, {"family": "Kailasan", "given": "Shweta", "initials": "S"}, {"family": "Aman", "given": "M Javad", "initials": "MJ"}, {"family": "Pin", "given": "Elisa", "initials": "E", "orcid": "0000-0002-2158-2674", "researcher": {"href": "https://publications.scilifelab.se/researcher/ccb4db02b9784587b62020716ab87247.json"}}, {"family": "Nilsson", "given": "Peter", "initials": "P", "orcid": "0000-0002-4657-8532", "researcher": {"href": "https://publications.scilifelab.se/researcher/799bcf1cf8cf451296f4535dd4ca9dc0.json"}}, {"family": "Thomas", "given": "Anne", "initials": "A", "orcid": "0000-0001-6901-3844", "researcher": {"href": "https://publications.scilifelab.se/researcher/3242b86e2de84178a74c1c199f1910f6.json"}}, {"family": "Viari", "given": "Alain", "initials": "A", "orcid": "0000-0002-1329-7777", "researcher": {"href": "https://publications.scilifelab.se/researcher/3b361fcd1ef94feaae3458693899a858.json"}}, {"family": "Sanlaville", "given": "Damien", "initials": "D"}, {"family": "Schneider", "given": "Francis", "initials": "F"}, {"family": "Sibilia", "given": "Jean", "initials": "J"}, {"family": "Tharaux", "given": "Pierre Louis", "initials": "PL", "orcid": "0000-0002-6062-5905", "researcher": {"href": "https://publications.scilifelab.se/researcher/5d6e1c79e38f4a4d83c19dcd0def5f32.json"}}, {"family": "Casanova", "given": "Jean Laurent", "initials": "JL", "orcid": "0000-0002-7782-4169", "researcher": {"href": "https://publications.scilifelab.se/researcher/009e2306468648c08fb1eea319f0c488.json"}}, {"family": "Hansmann", "given": "Yves", "initials": "Y", "orcid": "0000-0001-8903-0027", "researcher": {"href": "https://publications.scilifelab.se/researcher/1e05e2d28e154aa5a19eacd5690e109d.json"}}, {"family": "Lidar", "given": "Daniel", "initials": "D", "orcid": "0000-0002-1671-1515", "researcher": {"href": "https://publications.scilifelab.se/researcher/d213f3e38f4d40b49056ff57ae662764.json"}}, {"family": "Radosavljevic", "given": "Mirjana", "initials": "M", "orcid": "0000-0003-3925-0640", "researcher": {"href": "https://publications.scilifelab.se/researcher/b455278e8bf7496f8beff983485dace4.json"}}, {"family": "Gulcher", "given": "Jeffrey R", "initials": "JR"}, {"family": "Meziani", "given": "Ferhat", "initials": "F"}, {"family": "Moog", "given": "Christiane", "initials": "C", "orcid": "0000-0002-0916-156X", "researcher": {"href": "https://publications.scilifelab.se/researcher/fe7d5553a9d24a0b83ad2f7ecc18a526.json"}}, {"family": "Chittenden", "given": "Thomas W", "initials": "TW"}, {"family": "Bahram", "given": "Seiamak", "initials": "S", "orcid": "0000-0002-6928-9952", "researcher": {"href": "https://publications.scilifelab.se/researcher/151805ef1f8142baab4c253b50680d18.json"}}], "type": "journal-article", "published": "2022-01-19", "journal": {"title": "Sci. Transl. Med.", "issn": "1946-6234", "pages": "eabj7521", "volume": "14", "issue": "628", "issn-l": "1946-6234"}, "abstract": "The drivers of critical coronavirus disease 2019 (COVID-19) remain unknown. Given major confounding factors such as age and comorbidities, true mediators of this condition have remained elusive. We used a multi-omics analysis combined with artificial intelligence in a young patient cohort where major comorbidities were excluded at the onset. The cohort included 47 \u201ccritical\u201d (in the intensive care unit under mechanical ventilation) and 25 \u201cnon-critical\u201d (in a non-critical care ward) patients with COVID-19 and 22 healthy individuals. The analyses included whole-genome sequencing, whole-blood RNA sequencing, plasma and blood mononuclear cell proteomics, cytokine profiling, and high-throughput immunophenotyping. An ensemble of machine learning, deep learning, quantum annealing, and structural causal modeling were used. Patients with critical COVID-19 were characterized by exacerbated inflammation, perturbed lymphoid and myeloid compartments, increased coagulation, and viral cell biology. Among differentially expressed genes, we observed up-regulation of the metalloprotease ADAM9. This gene signature was validated in a second independent cohort of 81 critical and 73 recovered patients with COVID-19 and was further confirmed at the transcriptional and protein level and by proteolytic activity. Ex vivo ADAM9 inhibition decreased severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) uptake and replication in human lung epithelial cells. In conclusion, within a young, otherwise healthy, cohort of individuals with COVID-19, we provide the landscape of biological perturbations in vivo where a unique gene signature differentiated critical from non-critical patients. We further identified ADAM9 as a driver of disease severity and a candidate therapeutic target.", "doi": "10.1126/scitranslmed.abj7521", "pmid": "34698500", "labels": {"Autoimmunity and Serology Profiling": "Service"}, "xrefs": [], "notes": [], "created": "2021-11-09T06:20:06.651Z", "modified": "2023-06-19T11:35:21.919Z"}, {"entity": "publication", "iuid": "40debb001133430dacf91ed1b897a801", "links": {"self": {"href": "https://publications.scilifelab.se/publication/40debb001133430dacf91ed1b897a801.json"}, "display": {"href": "https://publications.scilifelab.se/publication/40debb001133430dacf91ed1b897a801"}}, "title": "Salivary IgG to SARS-CoV-2 indicates seroconversion and correlates to serum neutralization in mRNA-vaccinated immunocompromised individuals", "authors": [{"family": "Healy", "given": "Katie", "initials": "K"}, {"family": "Pin", "given": "Elisa", "initials": "E", "orcid": "0000-0002-2158-2674", "researcher": {"href": "https://publications.scilifelab.se/researcher/ccb4db02b9784587b62020716ab87247.json"}}, {"family": "Chen", "given": "Puran", "initials": "P"}, {"family": "S\u00f6derdahl", "given": "Gunnar", "initials": "G"}, {"family": "Nowak", "given": "Piotr", "initials": "P"}, {"family": "Mielke", "given": "Stephan", "initials": "S", "orcid": "0000-0002-8325-9215", "researcher": {"href": "https://publications.scilifelab.se/researcher/dbb6778eb9964f9cbd9ffc260892d609.json"}}, {"family": "Hansson", "given": "Lotta", "initials": "L"}, {"family": "Bergman", "given": "Peter", "initials": "P", "orcid": "0000-0003-3306-3713", "researcher": {"href": "https://publications.scilifelab.se/researcher/397d11713c80456bb600b1e4c88ff843.json"}}, {"family": "Smith", "given": "C I Edvard", "initials": "CIE"}, {"family": "Ljungman", "given": "Per", "initials": "P"}, {"family": "Valentini", "given": "Davide", "initials": "D"}, {"family": "Blennow", "given": "Ola", "initials": "O", "orcid": "0000-0002-7167-7882", "researcher": {"href": "https://publications.scilifelab.se/researcher/08ed03f808e84f50a0b599ce28b29d7d.json"}}, {"family": "\u00d6sterborg", "given": "Anders", "initials": "A"}, {"family": "Gabarrini", "given": "Giorgio", "initials": "G", "orcid": "0000-0001-6936-4919", "researcher": {"href": "https://publications.scilifelab.se/researcher/dbb92481579b4a23afefbbed5ad59a9a.json"}}, {"family": "Al-Manei", "given": "Khaled", "initials": "K", "orcid": "0000-0003-0787-5849", "researcher": {"href": "https://publications.scilifelab.se/researcher/289ad83f90da43adb6634178107db3de.json"}}, {"family": "Alkharaan", "given": "Hassan", "initials": "H"}, {"family": "Sobkowiak", "given": "Michal Jacek", "initials": "MJ", "orcid": "0000-0003-2932-1994", "researcher": {"href": "https://publications.scilifelab.se/researcher/14f1eb990edf472cac56f24d7cae330c.json"}}, {"family": "Yousef", "given": "Jamil", "initials": "J"}, {"family": "Mravinacova", "given": "Sara", "initials": "S"}, {"family": "Cuapio", "given": "Angelica", "initials": "A"}, {"family": "Xu", "given": "Xinling", "initials": "X"}, {"family": "Akber", "given": "Mira", "initials": "M"}, {"family": "Lor\u00e9", "given": "Karin", "initials": "K", "orcid": "0000-0001-7679-9494", "researcher": {"href": "https://publications.scilifelab.se/researcher/737245a5bc2144bd90be71487cbb3192.json"}}, {"family": "Hellstr\u00f6m", "given": "Cecilia", "initials": "C", "orcid": "0000-0003-0880-5375", "researcher": {"href": "https://publications.scilifelab.se/researcher/dbf3f75938f0442a9b1ae5c98565f44a.json"}}, {"family": "Muschiol", "given": "Sandra", "initials": "S"}, {"family": "Bogdanovic", "given": "Gordana", "initials": "G"}, {"family": "Buggert", "given": "Marcus", "initials": "M", "orcid": "0000-0003-0633-1719", "researcher": {"href": "https://publications.scilifelab.se/researcher/9a54e4b5136642eeafa77ac5118a0c81.json"}}, {"family": "Ljunggren", "given": "Hans Gustaf", "initials": "HG"}, {"family": "Hober", "given": "Sophia", "initials": "S", "orcid": "0000-0003-0605-8417", "researcher": {"href": "https://publications.scilifelab.se/researcher/f8dd8ee4264d4e4b912dacad3106f40a.json"}}, {"family": "Nilsson", "given": "Peter", "initials": "P", "orcid": "0000-0002-4657-8532", "researcher": {"href": "https://publications.scilifelab.se/researcher/799bcf1cf8cf451296f4535dd4ca9dc0.json"}}, {"family": "Aleman", "given": "Soo", "initials": "S", "orcid": "0000-0003-0461-4870", "researcher": {"href": "https://publications.scilifelab.se/researcher/d8a125a916b34de985de977dd8384650.json"}}, {"family": "Chen", "given": "Margaret S\u00e4llberg", "initials": "MS"}], "type": "journal-article", "published": "2022-01-00", "journal": {"title": "Med", "issn": "2666-6340", "issn-l": null, "volume": null, "issue": null, "pages": null}, "abstract": null, "doi": "10.1016/j.medj.2022.01.001", "pmid": "35075450", "labels": {"Autoimmunity and Serology Profiling": "Collaborative"}, "xrefs": [], "notes": [], "created": "2022-01-26T08:48:19.747Z", "modified": "2022-01-26T08:51:23.010Z"}, {"entity": "publication", "iuid": "662e6300d57a45818e6a52bf43aa890a", "links": {"self": {"href": "https://publications.scilifelab.se/publication/662e6300d57a45818e6a52bf43aa890a.json"}, "display": {"href": "https://publications.scilifelab.se/publication/662e6300d57a45818e6a52bf43aa890a"}}, "title": "Robust humoral and cellular immune responses and low risk for reinfection at least 8 months following asymptomatic to mild COVID-19.", "authors": [{"family": "Havervall", "given": "Sebastian", "initials": "S", "orcid": "0000-0003-1671-8183", "researcher": {"href": "https://publications.scilifelab.se/researcher/dbd194b3cef64f9f9341fd163a035235.json"}}, {"family": "Ng", "given": "Henry", "initials": "H", "orcid": "0000-0003-2873-9088", "researcher": {"href": "https://publications.scilifelab.se/researcher/5fcb12c664a64724b5cd42a1267a5bea.json"}}, {"family": "Jernbom Falk", "given": "August", "initials": "A", "orcid": "0000-0002-7773-1851", "researcher": {"href": "https://publications.scilifelab.se/researcher/fe6400bde68b46899515cef5bea05fca.json"}}, {"family": "Greilert-Norin", "given": "Nina", "initials": "N", "orcid": "0000-0003-1492-9745", "researcher": {"href": "https://publications.scilifelab.se/researcher/cd0c03be884e4ae8840d0e0d04c411dd.json"}}, {"family": "M\u00e5nberg", "given": "Anna", "initials": "A", "orcid": "0000-0002-0056-1313", "researcher": {"href": "https://publications.scilifelab.se/researcher/6d155273b5b54e61b773f263e4f2ce9b.json"}}, {"family": "Marking", "given": "Ulrika", "initials": "U"}, {"family": "Laur\u00e9n", "given": "Ida", "initials": "I"}, {"family": "Gabrielsson", "given": "Lena", "initials": "L"}, {"family": "Salomonsson", "given": "Ann-Christin", "initials": "AC"}, {"family": "Aguilera", "given": "Katherina", "initials": "K"}, {"family": "Kihlgren", "given": "Martha", "initials": "M"}, {"family": "M\u00e5nsson", "given": "Maja", "initials": "M"}, {"family": "Rosell", "given": "Axel", "initials": "A", "orcid": "0000-0001-6280-0562", "researcher": {"href": "https://publications.scilifelab.se/researcher/bcddaba7a96a406b994756ab71427baf.json"}}, {"family": "Hellstr\u00f6m", "given": "Cecilia", "initials": "C", "orcid": "0000-0003-0880-5375", "researcher": {"href": "https://publications.scilifelab.se/researcher/dbf3f75938f0442a9b1ae5c98565f44a.json"}}, {"family": "Andersson", "given": "Eni", "initials": "E"}, {"family": "Olofsson", "given": "Jennie", "initials": "J", "orcid": "0000-0002-8593-9089", "researcher": {"href": "https://publications.scilifelab.se/researcher/d2b59febdeec4df99bf3a0d5480df305.json"}}, {"family": "Skoglund", "given": "Lovisa", "initials": "L"}, {"family": "Yousef", "given": "Jamil", "initials": "J", "orcid": "0000-0001-5915-1258", "researcher": {"href": "https://publications.scilifelab.se/researcher/86be17c932614797a876bb55c7ce566e.json"}}, {"family": "Pin", "given": "Elisa", "initials": "E", "orcid": "0000-0002-2158-2674", "researcher": {"href": "https://publications.scilifelab.se/researcher/ccb4db02b9784587b62020716ab87247.json"}}, {"family": "Lord", "given": "Martin", "initials": "M", "orcid": "0000-0002-3238-3187", "researcher": {"href": "https://publications.scilifelab.se/researcher/3e14847c142f4c359f2d531601436897.json"}}, {"family": "\u00c5berg", "given": "Mikael", "initials": "M", "orcid": "0000-0002-7858-8233", "researcher": {"href": "https://publications.scilifelab.se/researcher/90fa86e9aeaa43ea9547e48b4f3f24e3.json"}}, {"family": "Hedhammar", "given": "My", "initials": "M"}, {"family": "Tegel", "given": "Hanna", "initials": "H"}, {"family": "D\u00f6nnes", "given": "Pierre", "initials": "P", "orcid": "0000-0002-4613-2952", "researcher": {"href": "https://publications.scilifelab.se/researcher/56d32d60f9b547be8096c448fc013246.json"}}, {"family": "Phillipson", "given": "Mia", "initials": "M", "orcid": "0000-0002-2387-0266", "researcher": {"href": "https://publications.scilifelab.se/researcher/1ebf9ffcab3e4a19add4c6dd51b727b1.json"}}, {"family": "Nilsson", "given": "Peter", "initials": "P", "orcid": "0000-0002-4657-8532", "researcher": {"href": "https://publications.scilifelab.se/researcher/799bcf1cf8cf451296f4535dd4ca9dc0.json"}}, {"family": "Klingstr\u00f6m", "given": "Jonas", "initials": "J", "orcid": "0000-0001-9076-1441", "researcher": {"href": "https://publications.scilifelab.se/researcher/95c1b345ae434fb383b7fe6a1d053c80.json"}}, {"family": "Mangsbo", "given": "Sara", "initials": "S", "orcid": "0000-0002-1355-2678", "researcher": {"href": "https://publications.scilifelab.se/researcher/c743bbcad6554f049c8fc5f64a6801bc.json"}}, {"family": "Hober", "given": "Sophia", "initials": "S", "orcid": "0000-0003-0605-8417", "researcher": {"href": "https://publications.scilifelab.se/researcher/f8dd8ee4264d4e4b912dacad3106f40a.json"}}, {"family": "Th\u00e5lin", "given": "Charlotte", "initials": "C", "orcid": "0000-0002-1345-6491", "researcher": {"href": "https://publications.scilifelab.se/researcher/130fb6ef6b774613a767e98f9f9b2eb4.json"}}], "type": "journal article", "published": "2022-01-00", "journal": {"title": "J. Intern. Med.", "issn": "1365-2796", "issn-l": "0954-6820", "volume": "291", "issue": "1", "pages": "72-80"}, "abstract": "Emerging data support detectable immune responses for months after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and vaccination, but it is not yet established to what degree and for how long protection against reinfection lasts.\n\nWe investigated SARS-CoV-2-specific humoral and cellular immune responses more than 8 months post-asymptomatic, mild and severe infection in a cohort of 1884 healthcare workers (HCW) and 51 hospitalized COVID-19 patients. Possible protection against SARS-CoV-2 reinfection was analyzed by a weekly 3-month polymerase chain reaction (PCR) screening of 252 HCW that had seroconverted 7 months prior to start of screening and 48 HCW that had remained seronegative at multiple time points.\n\nAll COVID-19 patients and 96% (355/370) of HCW who were anti-spike IgG positive at inclusion remained anti-spike IgG positive at the 8-month follow-up. Circulating SARS-CoV-2-specific memory T cell responses were detected in 88% (45/51) of COVID-19 patients and in 63% (233/370) of seropositive HCW. The cumulative incidence of PCR-confirmed SARS-CoV-2 infection was 1% (3/252) among anti-spike IgG positive HCW (0.13 cases per 100 weeks at risk) compared to 23% (11/48) among anti-spike IgG negative HCW (2.78 cases per 100 weeks at risk), resulting in a protective effect of 95.2% (95% CI 81.9%-99.1%).\n\nThe vast majority of anti-spike IgG positive individuals remain anti-spike IgG positive for at least 8 months regardless of initial COVID-19 disease severity. The presence of anti-spike IgG antibodies is associated with a substantially reduced risk of reinfection up to 9 months following asymptomatic to mild COVID-19.", "doi": "10.1111/joim.13387", "pmid": "34459525", "labels": {"Autoimmunity and Serology Profiling": "Collaborative", "Affinity Proteomics Uppsala": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC8661920"}], "notes": [], "created": "2021-08-31T10:38:04.811Z", "modified": "2022-12-02T10:46:29.165Z"}, {"entity": "publication", "iuid": "9d0f9b6ced5447dfab67df3380c65330", "links": {"self": {"href": "https://publications.scilifelab.se/publication/9d0f9b6ced5447dfab67df3380c65330.json"}, "display": {"href": "https://publications.scilifelab.se/publication/9d0f9b6ced5447dfab67df3380c65330"}}, "title": "Systematic evaluation of SARS-CoV-2 antigens enables a highly specific and sensitive multiplex serological COVID-19 assay.", "authors": [{"family": "Hober", "given": "Sophia", "initials": "S", "orcid": "0000-0003-0605-8417", "researcher": {"href": "https://publications.scilifelab.se/researcher/f8dd8ee4264d4e4b912dacad3106f40a.json"}}, {"family": "Hellstr\u00f6m", "given": "Cecilia", "initials": "C"}, {"family": "Olofsson", "given": "Jennie", "initials": "J", "orcid": "0000-0002-8593-9089", "researcher": {"href": "https://publications.scilifelab.se/researcher/d2b59febdeec4df99bf3a0d5480df305.json"}}, {"family": "Andersson", "given": "Eni", "initials": "E", "orcid": "0000-0002-5115-0637", "researcher": {"href": "https://publications.scilifelab.se/researcher/ef94cf857c3746088cd01cee49242f24.json"}}, {"family": "Bergstr\u00f6m", "given": "Sofia", "initials": "S", "orcid": "0000-0003-2910-4754", "researcher": {"href": "https://publications.scilifelab.se/researcher/648c9ed3483a4eb8a1d228cf7e59f6a7.json"}}, {"family": "Jernbom Falk", "given": "August", "initials": "A", "orcid": "0000-0002-7773-1851", "researcher": {"href": "https://publications.scilifelab.se/researcher/fe6400bde68b46899515cef5bea05fca.json"}}, {"family": "Bayati", "given": "Shaghayegh", "initials": "S"}, {"family": "Mravinacova", "given": "Sara", "initials": "S"}, {"family": "Sj\u00f6berg", "given": "Ronald", "initials": "R", "orcid": "0000-0003-1363-5796", "researcher": {"href": "https://publications.scilifelab.se/researcher/d08326da26da422ab445a26563843e79.json"}}, {"family": "Yousef", "given": "Jamil", "initials": "J", "orcid": "0000-0001-5915-1258", "researcher": {"href": "https://publications.scilifelab.se/researcher/86be17c932614797a876bb55c7ce566e.json"}}, {"family": "Skoglund", "given": "Lovisa", "initials": "L"}, {"family": "Kanje", "given": "Sara", "initials": "S"}, {"family": "Berling", "given": "Anna", "initials": "A"}, {"family": "Svensson", "given": "Anne-Sophie", "initials": "AS"}, {"family": "Jensen", "given": "Gabriella", "initials": "G"}, {"family": "Enstedt", "given": "Henric", "initials": "H"}, {"family": "Afshari", "given": "Delaram", "initials": "D"}, {"family": "Xu", "given": "Lan Lan", "initials": "LL"}, {"family": "Zwahlen", "given": "Martin", "initials": "M"}, {"family": "von Feilitzen", "given": "Kalle", "initials": "K"}, {"family": "Hanke", "given": "Leo", "initials": "L"}, {"family": "Murrell", "given": "Ben", "initials": "B"}, {"family": "McInerney", "given": "Gerald", "initials": "G"}, {"family": "Karlsson Hedestam", "given": "Gunilla B", "initials": "GB"}, {"family": "Lendel", "given": "Christofer", "initials": "C"}, {"family": "Roth", "given": "Robert G", "initials": "RG"}, {"family": "Skoog", "given": "Ingmar", "initials": "I"}, {"family": "Svenungsson", "given": "Elisabet", "initials": "E"}, {"family": "Olsson", "given": "Tomas", "initials": "T"}, {"family": "Fogdell-Hahn", "given": "Anna", "initials": "A"}, {"family": "Lindroth", "given": "Ylva", "initials": "Y"}, {"family": "Lundgren", "given": "Maria", "initials": "M"}, {"family": "Maleki", "given": "Kimia T", "initials": "KT"}, {"family": "Lagerqvist", "given": "Nina", "initials": "N"}, {"family": "Klingstr\u00f6m", "given": "Jonas", "initials": "J", "orcid": "0000-0001-9076-1441", "researcher": {"href": "https://publications.scilifelab.se/researcher/95c1b345ae434fb383b7fe6a1d053c80.json"}}, {"family": "Da Silva Rodrigues", "given": "Rui", "initials": "R"}, {"family": "Muschiol", "given": "Sandra", "initials": "S"}, {"family": "Bogdanovic", "given": "Gordana", "initials": "G"}, {"family": "Arroyo M\u00fchr", "given": "Laila Sara", "initials": "LS"}, {"family": "Eklund", "given": "Carina", "initials": "C"}, {"family": "Lagheden", "given": "Camilla", "initials": "C"}, {"family": "Dillner", "given": "Joakim", "initials": "J"}, {"family": "Sivertsson", "given": "\u00c5sa", "initials": "\u00c5"}, {"family": "Havervall", "given": "Sebastian", "initials": "S", "orcid": "0000-0003-1671-8183", "researcher": {"href": "https://publications.scilifelab.se/researcher/dbd194b3cef64f9f9341fd163a035235.json"}}, {"family": "Th\u00e5lin", "given": "Charlotte", "initials": "C", "orcid": "0000-0002-1345-6491", "researcher": {"href": "https://publications.scilifelab.se/researcher/130fb6ef6b774613a767e98f9f9b2eb4.json"}}, {"family": "Tegel", "given": "Hanna", "initials": "H", "orcid": "0000-0002-7067-9173", "researcher": {"href": "https://publications.scilifelab.se/researcher/d3d733dbd7b84a6b88f7f5fcff7165f6.json"}}, {"family": "Pin", "given": "Elisa", "initials": "E", "orcid": "0000-0002-2158-2674", "researcher": {"href": "https://publications.scilifelab.se/researcher/ccb4db02b9784587b62020716ab87247.json"}}, {"family": "M\u00e5nberg", "given": "Anna", "initials": "A", "orcid": "0000-0002-0056-1313", "researcher": {"href": "https://publications.scilifelab.se/researcher/6d155273b5b54e61b773f263e4f2ce9b.json"}}, {"family": "Hedhammar", "given": "My", "initials": "M", "orcid": "0000-0003-0140-419X", "researcher": {"href": "https://publications.scilifelab.se/researcher/061cceecaf0e4bed8efabca146e5b235.json"}}, {"family": "Nilsson", "given": "Peter", "initials": "P", "orcid": "0000-0002-4657-8532", "researcher": {"href": "https://publications.scilifelab.se/researcher/799bcf1cf8cf451296f4535dd4ca9dc0.json"}}], "type": "journal article", "published": "2021-07-19", "journal": {"title": "Clin Transl Immunology", "issn": "2050-0068", "volume": "10", "issue": "7", "pages": "e1312", "issn-l": null}, "abstract": "The COVID-19 pandemic poses an immense need for accurate, sensitive and high-throughput clinical tests, and serological assays are needed for both overarching epidemiological studies and evaluating vaccines. Here, we present the development and validation of a high-throughput multiplex bead-based serological assay.\n\nMore than 100 representations of SARS-CoV-2 proteins were included for initial evaluation, including antigens produced in bacterial and mammalian hosts as well as synthetic peptides. The five best-performing antigens, three representing the spike glycoprotein and two representing the nucleocapsid protein, were further evaluated for detection of IgG antibodies in samples from 331 COVID-19 patients and convalescents, and in 2090 negative controls sampled before 2020.\n\nThree antigens were finally selected, represented by a soluble trimeric form and the S1-domain of the spike glycoprotein as well as by the C-terminal domain of the nucleocapsid. The sensitivity for these three antigens individually was found to be 99.7%, 99.1% and 99.7%, and the specificity was found to be 98.1%, 98.7% and 95.7%. The best assay performance was although achieved when utilising two antigens in combination, enabling a sensitivity of up to 99.7% combined with a specificity of 100%. Requiring any two of the three antigens resulted in a sensitivity of 99.7% and a specificity of 99.4%.\n\nThese observations demonstrate that a serological test based on a combination of several SARS-CoV-2 antigens enables a highly specific and sensitive multiplex serological COVID-19 assay.", "doi": "10.1002/cti2.1312", "pmid": "34295471", "labels": {"Autoimmunity and Serology Profiling": "Technology development"}, "xrefs": [{"db": "pii", "key": "CTI21312"}, {"db": "pmc", "key": "PMC8288725"}], "notes": [], "created": "2021-07-25T21:30:22.465Z", "modified": "2021-11-10T12:23:19.192Z"}, {"entity": "publication", "iuid": "c4121567e5bb46d5bfee7bf4443780a2", "links": {"self": {"href": "https://publications.scilifelab.se/publication/c4121567e5bb46d5bfee7bf4443780a2.json"}, "display": {"href": "https://publications.scilifelab.se/publication/c4121567e5bb46d5bfee7bf4443780a2"}}, "title": "Multiplexed analysis of the secretin-like GPCR-RAMP interactome.", "authors": [{"family": "Lorenzen", "given": "Emily", "initials": "E", "orcid": "0000-0002-0008-0966", "researcher": {"href": "https://publications.scilifelab.se/researcher/e009a3686e804b808372ef5cd3eabaf3.json"}}, {"family": "Dodig-Crnkovi\u0107", "given": "Tea", "initials": "T", "orcid": "0000-0002-2875-896X", "researcher": {"href": "https://publications.scilifelab.se/researcher/cf18af5b676b449693945249fc1767e4.json"}}, {"family": "Kotliar", "given": "Ilana B", "initials": "IB", "orcid": "0000-0001-9533-4828", "researcher": {"href": "https://publications.scilifelab.se/researcher/ddf97aaf7767453a9a165e6198fda679.json"}}, {"family": "Pin", "given": "Elisa", "initials": "E", "orcid": "0000-0002-2158-2674", "researcher": {"href": "https://publications.scilifelab.se/researcher/ccb4db02b9784587b62020716ab87247.json"}}, {"family": "Ceraudo", "given": "Emilie", "initials": "E", "orcid": "0000-0001-6563-9751", "researcher": {"href": "https://publications.scilifelab.se/researcher/58ba695a85a84fe2a7986ea3f3eff4ab.json"}}, {"family": "Vaughan", "given": "Roger D", "initials": "RD"}, {"family": "Uhl\u00e8n", "given": "Mathias", "initials": "M", "orcid": "0000-0002-4858-8056", "researcher": {"href": "https://publications.scilifelab.se/researcher/ff81da3cb0cf4262873b993a1b06798c.json"}}, {"family": "Huber", "given": "Thomas", "initials": "T", "orcid": "0000-0002-1844-262X", "researcher": {"href": "https://publications.scilifelab.se/researcher/f730be20f6dd4b8db1756bfed4aa1170.json"}}, {"family": "Schwenk", "given": "Jochen M", "initials": "JM", "orcid": "0000-0001-8141-8449", "researcher": {"href": "https://publications.scilifelab.se/researcher/aba5822711b246b397fffacb7ae403b3.json"}}, {"family": "Sakmar", "given": "Thomas P", "initials": "TP", "orcid": "0000-0002-2836-8953", "researcher": {"href": "https://publications.scilifelab.se/researcher/1130157044664f8b994b94e3b199cc30.json"}}], "type": "journal article", "published": "2019-09-00", "journal": {"title": "Sci Adv", "issn": "2375-2548", "volume": "5", "issue": "9", "pages": "eaaw2778", "issn-l": "2375-2548"}, "abstract": "Receptor activity-modifying proteins (RAMPs) have been shown to modulate the functions of several G protein-coupled receptors (GPCRs), but potential direct interactions among the three known RAMPs and hundreds of GPCRs have never been investigated. Focusing mainly on the secretin-like family of GPCRs, we engineered epitope-tagged GPCRs and RAMPs, and developed a multiplexed suspension bead array (SBA) immunoassay to detect GPCR-RAMP complexes from detergent-solubilized lysates. Using 64 antibodies raised against the native proteins and 4 antibodies targeting the epitope tags, we mapped the interactions among 23 GPCRs and 3 RAMPs. We validated nearly all previously reported secretin-like GPCR-RAMP interactions, and also found previously unidentified RAMP interactions with additional secretin-like GPCRs, chemokine receptors, and orphan receptors. The results provide a complete interactome of secretin-like GPCRs with RAMPs. The SBA strategy will be useful to search for additional GPCR-RAMP complexes and other interacting membrane protein pairs in cell lines and tissues.", "doi": "10.1126/sciadv.aaw2778", "pmid": "31555726", "labels": {"Affinity Proteomics Stockholm": "Collaborative"}, "xrefs": [{"db": "pii", "key": "aaw2778"}, {"db": "pmc", "key": "PMC6750928"}], "notes": [], "created": "2020-01-08T10:24:44.506Z", "modified": "2021-07-08T12:07:33.919Z"}]}