{"entity": "researcher", "timestamp": "2026-04-16T17:43:20.447Z", "family": "Baltzer", "given": "Lars", "initials": "L", "orcid": "0000-0001-5807-2726", "affiliations": ["Department of Chemistry-BMC", "Uppsala University", "Uppsala", "Sweden"], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/ca711c9ac6fd43e4baf6e95886c85774.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/ca711c9ac6fd43e4baf6e95886c85774"}}, "publications": [{"entity": "publication", "iuid": "64b356ba093a4bf180f042a619c896a0", "links": {"self": {"href": "https://publications.scilifelab.se/publication/64b356ba093a4bf180f042a619c896a0.json"}, "display": {"href": "https://publications.scilifelab.se/publication/64b356ba093a4bf180f042a619c896a0"}}, "title": "High-affinity recognition of the human C-reactive protein independent of phosphocholine.", "authors": [{"family": "Yang", "given": "Jie", "initials": "J"}, {"family": "Gustavsson", "given": "Anna-Lena", "initials": "AL", "orcid": "0000-0003-4332-2336", "researcher": {"href": "https://publications.scilifelab.se/researcher/6b014ef7ea0d461b8e2ddb87506b1252.json"}}, {"family": "Haraldsson", "given": "Martin", "initials": "M"}, {"family": "Karlsson", "given": "G\u00f6ran", "initials": "G", "orcid": "0000-0002-1821-4715", "researcher": {"href": "https://publications.scilifelab.se/researcher/2c6463abd05b415696c52be577ca2be6.json"}}, {"family": "Norberg", "given": "Thomas", "initials": "T", "orcid": "0000-0002-0675-7675", "researcher": {"href": "https://publications.scilifelab.se/researcher/a85dcf5837e641a4a844401b54dae2d9.json"}}, {"family": "Baltzer", "given": "Lars", "initials": "L", "orcid": "0000-0001-5807-2726", "researcher": {"href": "https://publications.scilifelab.se/researcher/ca711c9ac6fd43e4baf6e95886c85774.json"}}], "type": "journal article", "published": "2017-05-31", "journal": {"volume": "15", "issn": "1477-0539", "issue": "21", "pages": "4644-4654", "title": "Org. Biomol. Chem.", "issn-l": "1477-0520"}, "abstract": "A high-affinity polypeptide conjugate 4-C25L22-DQ, has been developed for the molecular recognition of the human C-reactive protein, CRP, a well-known inflammation biomarker. CRP is one of the most frequently quantified targets in diagnostic applications and a target in drug development. With the exception of antibodies, most molecular constructs take advantage of the known affinity for CRP of phosphocholine that depends on Ca2+ for its ability to bind. 4-C25L22-DQ which is unrelated to phosphocholine binds in the absence of Ca2+ with a dissociation constant of 760 nM, an order of magnitude lower than that of phosphocholine, the KD of which is 5 \u03bcM. The small organic molecule 2-oxo-1,2-dihydroquinoline-8-carboxylic acid (DQ) was designed based on the structural similarities between three hits from a set of compounds selected from a building block collection and evaluated with regards to affinity for CRP by NMR spectroscopy. 4-C25L22-DQ was shown in a competition experiment to bind CRP three orders of magnitude more strongly than DQ itself, and in a pull-down experiment 4-C25L22-DQ was shown to extract CRP from human serum. The development of a robust and phosphocholine-independent recognition element provides unprecedented opportunities in bioanalytical applications in vivo and in vitro under conditions where the concentration of Ca2+ ions is low, or where Ca2+ binding agents such as EDTA or heparin are needed to prevent blood coagulation. The identification from a compound library of a small organic molecule and its conjugation to a small set of polypeptides, none of which were previously known to bind CRP, illustrates a convenient and general route to selective high-affinity binders for proteins with dissociation constants in the \u03bcM to nM range for which no small molecule ligands are known.", "doi": "10.1039/c7ob00684e", "pmid": "28513744", "labels": {"Swedish NMR Centre": "Service", "Chemical Biology Consortium Sweden": "Collaborative"}, "xrefs": [], "notes": [], "created": "2017-10-20T15:12:12.567Z", "modified": "2025-10-17T13:04:29.262Z"}]}