{"entity": "researcher", "timestamp": "2026-04-14T21:15:41.905Z", "family": "Frisk", "given": "Thomas W", "initials": "TW", "orcid": "0000-0002-3996-9279", "affiliations": ["Biophysics, Department of Applied Physics, Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/c8a1aeeab11e4180afd6551faf16ab9f.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/c8a1aeeab11e4180afd6551faf16ab9f"}}, "publications": [{"entity": "publication", "iuid": "0fbeef5aada44fa29aa458238f0272a3", "links": {"self": {"href": "https://publications.scilifelab.se/publication/0fbeef5aada44fa29aa458238f0272a3.json"}, "display": {"href": "https://publications.scilifelab.se/publication/0fbeef5aada44fa29aa458238f0272a3"}}, "title": "A thermoplastic chip for 2D and 3D correlative assays combining screening and high-resolution imaging of immune cell responses.", "authors": [{"family": "van Ooijen", "given": "Hanna", "initials": "H"}, {"family": "Verron", "given": "Quentin", "initials": "Q", "orcid": "0000-0001-5800-4379", "researcher": {"href": "https://publications.scilifelab.se/researcher/a961009c75cf42499996cfd89e7c46e5.json"}}, {"family": "Zhang", "given": "Hanqing", "initials": "H"}, {"family": "Sandoz", "given": "Patrick A", "initials": "PA", "orcid": "0000-0002-8379-7267", "researcher": {"href": "https://publications.scilifelab.se/researcher/acd737132e4b4b378257ae8f0316b3a2.json"}}, {"family": "Frisk", "given": "Thomas W", "initials": "TW", "orcid": "0000-0002-3996-9279", "researcher": {"href": "https://publications.scilifelab.se/researcher/c8a1aeeab11e4180afd6551faf16ab9f.json"}}, {"family": "Carannante", "given": "Valentina", "initials": "V"}, {"family": "Olofsson", "given": "Karl", "initials": "K"}, {"family": "Wagner", "given": "Arnika K", "initials": "AK", "orcid": "0000-0002-0339-8259", "researcher": {"href": "https://publications.scilifelab.se/researcher/8dd10981a93a490b940ca41d2bde96a3.json"}}, {"family": "Sandstr\u00f6m", "given": "Niklas", "initials": "N"}, {"family": "\u00d6nfelt", "given": "Bj\u00f6rn", "initials": "B", "orcid": "0000-0001-5178-7593", "researcher": {"href": "https://publications.scilifelab.se/researcher/14bcdcf94cab40ff92319869d243f5d8.json"}}], "type": "journal article", "published": "2025-01-16", "journal": {"title": "Cell Reports Methods", "issn": "2667-2375", "pages": "100965", "issn-l": null}, "abstract": "We present an easy-to-use, disposable, thermoplastic microwell chip designed to support screening and high-resolution imaging of single-cell behavior in two- and three-dimensional (2D and 3D) cell cultures. We show that the chip has excellent optical properties and provide simple protocols for efficient long-term cell culture of suspension and adherent cells, the latter grown either as monolayers or as hundreds of single, uniformly sized spheroids. We then demonstrate the applicability of the system for single-cell analysis by correlating the dynamic cytotoxic response of single immune cells grown under different metabolic conditions to their intracellular cytolytic load at the end of the assay. Additionally, we illustrate highly multiplex cytotoxicity screening of tumor spheroids in the chip, comparing the effect of environment cues characteristic of the tumor microenvironment on natural killer (NK)-cell-induced killing. Following the functional screening, we perform high-resolution 3D immunofluorescent imaging of infiltrating NK cells within the spheroid volumes.", "doi": "10.1016/j.crmeth.2025.100965", "pmid": "39826552", "labels": {"Integrated Microscopy Technologies Stockholm": "Service"}, "xrefs": [{"db": "pii", "key": "S2667-2375(25)00001-3"}], "notes": [], "created": "2025-01-23T11:28:33.594Z", "modified": "2025-04-07T07:24:00.541Z"}, {"entity": "publication", "iuid": "836d8b1e7ef94eb782ad4e2c1653e47a", "links": {"self": {"href": "https://publications.scilifelab.se/publication/836d8b1e7ef94eb782ad4e2c1653e47a.json"}, "display": {"href": "https://publications.scilifelab.se/publication/836d8b1e7ef94eb782ad4e2c1653e47a"}}, "title": "NK cells integrate signals over large areas when building immune synapses but require local stimuli for degranulation.", "authors": [{"family": "Verron", "given": "Quentin", "initials": "Q", "orcid": "0000-0001-5800-4379", "researcher": {"href": "https://publications.scilifelab.se/researcher/a961009c75cf42499996cfd89e7c46e5.json"}}, {"family": "Forslund", "given": "Elin", "initials": "E"}, {"family": "Brandt", "given": "Ludwig", "initials": "L", "orcid": "0000-0003-2040-3176", "researcher": {"href": "https://publications.scilifelab.se/researcher/a1683a18701c4e23ad7005a6672595f9.json"}}, {"family": "Leino", "given": "Mattias", "initials": "M", "orcid": "0000-0002-7533-043X", "researcher": {"href": "https://publications.scilifelab.se/researcher/88c2bd6f902f4b8c96a1855ab4bf12ab.json"}}, {"family": "Frisk", "given": "Thomas W", "initials": "TW", "orcid": "0000-0002-3996-9279", "researcher": {"href": "https://publications.scilifelab.se/researcher/c8a1aeeab11e4180afd6551faf16ab9f.json"}}, {"family": "Olofsson", "given": "Per E", "initials": "PE", "orcid": "0000-0002-6019-8157", "researcher": {"href": "https://publications.scilifelab.se/researcher/204c5891e333412cab14381b853c49a4.json"}}, {"family": "\u00d6nfelt", "given": "Bj\u00f6rn", "initials": "B", "orcid": "0000-0001-5178-7593", "researcher": {"href": "https://publications.scilifelab.se/researcher/14bcdcf94cab40ff92319869d243f5d8.json"}}], "type": "journal article", "published": "2021-05-25", "journal": {"title": "Sci Signal", "issn": "1937-9145", "issn-l": "1945-0877", "volume": "14", "issue": "684", "pages": "eabe2740"}, "abstract": "Immune synapses are large-scale, transient molecular assemblies that serve as platforms for antigen presentation to B and T cells and for target recognition by cytotoxic T cells and natural killer (NK) cells. The formation of an immune synapse is a tightly regulated, stepwise process in which the cytoskeleton, cell surface receptors, and intracellular signaling proteins rearrange into supramolecular activation clusters (SMACs). We generated artificial immune synapses (AIS) consisting of synthetic and natural ligands for the NK cell-activating receptors LFA-1 and CD16 by microcontact printing the ligands into circular-shaped SMAC structures. Live-cell imaging and analysis of fixed human NK cells in this reductionist system showed that the spatial distribution of activating ligands influenced the formation, stability, and outcome of NK cell synapses. Whereas engagement of LFA-1 alone promoted synapse initiation, combined engagement of LFA-1 and CD16 was required for the formation of mature synapses and degranulation. Organizing LFA-1 and CD16 ligands into donut-shaped AIS resulted in fewer long-lasting, symmetrical synapses compared to dot-shaped AIS. NK cells spreading evenly over either AIS shape exhibited similar arrangements of the lytic machinery. However, degranulation only occurred in regions containing ligands that therefore induced local signaling, suggesting the existence of a late checkpoint for degranulation. Our results demonstrate that the spatial organization of ligands in the synapse can affect its outcome, which could be exploited by target cells as an escape mechanism.", "doi": "10.1126/scisignal.abe2740", "pmid": "34035142", "labels": {"Integrated Microscopy Technologies Stockholm": "Service"}, "xrefs": [{"db": "pii", "key": "14/684/eabe2740"}], "notes": [], "created": "2021-05-26T07:17:06.650Z", "modified": "2021-11-10T12:24:36.755Z"}]}