{"entity": "researcher", "timestamp": "2026-06-16T12:34:56.175Z", "family": "Huiskonen", "given": "Juha T", "initials": "JT", "orcid": "0000-0002-0348-7323", "affiliations": ["Institute of Biotechnology, Helsinki Institute of Life Science HiLIFE, University of Helsinki, Helsinki, Finland. juha.huiskonen@helsinki.fi.", "Molecular and Integrative Biosciences Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland. juha.huiskonen@helsinki.fi.", "Division of Structural Biology, Wellcome Centre for Human Genetics, Roosevelt Drive, University of Oxford, Oxford, UK. juha.huiskonen@helsinki.fi."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/c858a2e60971462f9e53653320c9eb0f.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/c858a2e60971462f9e53653320c9eb0f"}}, "publications": [{"entity": "publication", "iuid": "a484c8a8f0c0429ab56b31b4e80db6e4", "links": {"self": {"href": "https://publications.scilifelab.se/publication/a484c8a8f0c0429ab56b31b4e80db6e4.json"}, "display": {"href": "https://publications.scilifelab.se/publication/a484c8a8f0c0429ab56b31b4e80db6e4"}}, "title": "Structure and interactions of the endogenous human Commander complex.", "authors": [{"family": "Laulumaa", "given": "Saara", "initials": "S", "orcid": "0000-0003-4836-9256", "researcher": {"href": "https://publications.scilifelab.se/researcher/0420474f746b42d2b451fdfd8ae578ed.json"}}, {"family": "Kumpula", "given": "Esa-Pekka", "initials": "EP", "orcid": "0000-0003-0335-5993", "researcher": {"href": "https://publications.scilifelab.se/researcher/eb426bc260a3414d9dabd2a1302813b1.json"}}, {"family": "Huiskonen", "given": "Juha T", "initials": "JT", "orcid": "0000-0002-0348-7323", "researcher": {"href": "https://publications.scilifelab.se/researcher/c858a2e60971462f9e53653320c9eb0f.json"}}, {"family": "Varjosalo", "given": "Markku", "initials": "M", "orcid": "0000-0002-1340-9732", "researcher": {"href": "https://publications.scilifelab.se/researcher/631a0a4964d747399c0b1781e546f0ab.json"}}], "type": "journal article", "published": "2024-06-00", "journal": {"title": "Nat. Struct. Mol. Biol.", "issn": "1545-9985", "volume": "31", "issue": "6", "pages": "925-938", "issn-l": "1545-9985"}, "abstract": "The Commander complex, a 16-protein assembly, plays multiple roles in cell homeostasis, cell cycle and immune response. It consists of copper-metabolism Murr1 domain proteins (COMMD1-10), coiled-coil domain-containing proteins (CCDC22 and CCDC93), DENND10 and the Retriever subcomplex (VPS26C, VPS29 and VPS35L), all expressed ubiquitously in the body and linked to various diseases. Here, we report the structure and key interactions of the endogenous human Commander complex by cryogenic-electron microscopy and mass spectrometry-based proteomics. The complex consists of a stable core of COMMD1-10 and an effector containing DENND10 and Retriever, scaffolded together by CCDC22 and CCDC93. We establish the composition of Commander and reveal major interaction interfaces. These findings clarify its roles in intracellular transport, and uncover a strong association with cilium assembly, and centrosome and centriole functions.", "doi": "10.1038/s41594-024-01246-1", "pmid": "38459129", "labels": {"Cryo-EM": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11189303"}, {"db": "pii", "key": "10.1038/s41594-024-01246-1"}], "notes": [], "created": "2024-11-15T13:40:35.645Z", "modified": "2024-11-19T07:36:48.332Z"}, {"entity": "publication", "iuid": "598feb16626f43cdb791a3f07b3a488b", "links": {"self": {"href": "https://publications.scilifelab.se/publication/598feb16626f43cdb791a3f07b3a488b.json"}, "display": {"href": "https://publications.scilifelab.se/publication/598feb16626f43cdb791a3f07b3a488b"}}, "title": "Molecular view of ER membrane remodeling by the Sec61/TRAP translocon.", "authors": [{"family": "Karki", "given": "Sudeep", "initials": "S", "orcid": "0000-0003-3851-1204", "researcher": {"href": "https://publications.scilifelab.se/researcher/2872667c8b6f400bb2373e19ef70e629.json"}}, {"family": "Javanainen", "given": "Matti", "initials": "M", "orcid": "0000-0003-4858-364X", "researcher": {"href": "https://publications.scilifelab.se/researcher/9d89f643000047efb67c8b68340601b9.json"}}, {"family": "Rehan", "given": "Shahid", "initials": "S", "orcid": "0000-0003-1329-8056", "researcher": {"href": "https://publications.scilifelab.se/researcher/5fe8a426af3d4750906f849a48526806.json"}}, {"family": "Tranter", "given": "Dale", "initials": "D"}, {"family": "Kellosalo", "given": "Juho", "initials": "J"}, {"family": "Huiskonen", "given": "Juha", "initials": "J", "orcid": "0000-0002-0348-7323", "researcher": {"href": "https://publications.scilifelab.se/researcher/c858a2e60971462f9e53653320c9eb0f.json"}}, {"family": "Happonen", "given": "Lotta", "initials": "L", "orcid": "0000-0002-5922-4549", "researcher": {"href": "https://publications.scilifelab.se/researcher/a7af0a6faf6a48e7937a644be472edbe.json"}}, {"family": "Paavilainen", "given": "Ville", "initials": "V", "orcid": "0000-0002-3160-7767", "researcher": {"href": "https://publications.scilifelab.se/researcher/f76359e8b92b4b53af80c36d064a1a42.json"}}], "type": "journal article", "published": "2023-11-20", "journal": {"title": "EMBO Rep.", "issn": "1469-3178", "pages": "e57910", "issn-l": "1469-221X"}, "abstract": "Protein translocation across the endoplasmic reticulum (ER) membrane is an essential step during protein entry into the secretory pathway. The conserved Sec61 protein-conducting channel facilitates polypeptide translocation and coordinates cotranslational polypeptide-processing events. In cells, the majority of Sec61 is stably associated with a heterotetrameric membrane protein complex, the translocon-associated protein complex (TRAP), yet the mechanism by which TRAP assists in polypeptide translocation remains unknown. Here, we present the structure of the core Sec61/TRAP complex bound to a mammalian ribosome by cryogenic electron microscopy (cryo-EM). Ribosome interactions anchor the Sec61/TRAP complex in a conformation that renders the ER membrane locally thinner by significantly curving its lumenal leaflet. We propose that TRAP stabilizes the ribosome exit tunnel to assist nascent polypeptide insertion through Sec61 and provides a ratcheting mechanism into the ER lumen mediated by direct polypeptide interactions.", "doi": "10.15252/embr.202357910", "pmid": "37983950", "labels": {"Structural Proteomics": "Collaborative"}, "xrefs": [{"db": "PDB", "key": "8BF9"}], "notes": [], "created": "2023-12-01T13:22:27.535Z", "modified": "2023-12-01T13:22:27.636Z"}, {"entity": "publication", "iuid": "b5c8c85e130e40d7984d2c097b23a4ea", "links": {"self": {"href": "https://publications.scilifelab.se/publication/b5c8c85e130e40d7984d2c097b23a4ea.json"}, "display": {"href": "https://publications.scilifelab.se/publication/b5c8c85e130e40d7984d2c097b23a4ea"}}, "title": "Intranasal trimeric sherpabody inhibits SARS-CoV-2 including recent immunoevasive Omicron subvariants.", "authors": [{"family": "M\u00e4kel\u00e4", "given": "Anna R", "initials": "AR"}, {"family": "U\u011furlu", "given": "Hasan", "initials": "H", "orcid": "0000-0002-3849-7847", "researcher": {"href": "https://publications.scilifelab.se/researcher/aa26094fded848c685b7ad9d2355b4dc.json"}}, {"family": "Hannula", "given": "Liina", "initials": "L"}, {"family": "Kant", "given": "Ravi", "initials": "R", "orcid": "0000-0003-3878-9775", "researcher": {"href": "https://publications.scilifelab.se/researcher/5ee44aebd7174ab9bb5fc741c0a13e2b.json"}}, {"family": "Salminen", "given": "Petja", "initials": "P"}, {"family": "Fagerlund", "given": "Riku", "initials": "R"}, {"family": "M\u00e4ki", "given": "Sanna", "initials": "S"}, {"family": "Haveri", "given": "Anu", "initials": "A", "orcid": "0000-0003-4620-7536", "researcher": {"href": "https://publications.scilifelab.se/researcher/981b0b4c9c9e47138958c7d8753d3340.json"}}, {"family": "Strandin", "given": "Tomas", "initials": "T"}, {"family": "Kareinen", "given": "Lauri", "initials": "L"}, {"family": "Hepojoki", "given": "Jussi", "initials": "J", "orcid": "0000-0001-5699-214X", "researcher": {"href": "https://publications.scilifelab.se/researcher/90f4bea163f34c08ade5950650ef9566.json"}}, {"family": "Kuivanen", "given": "Suvi", "initials": "S"}, {"family": "Levanov", "given": "Lev", "initials": "L"}, {"family": "Pasternack", "given": "Arja", "initials": "A", "orcid": "0000-0002-6088-4245", "researcher": {"href": "https://publications.scilifelab.se/researcher/1e8c1a42861e49a99be70101486ed3b1.json"}}, {"family": "Naves", "given": "Rauno A", "initials": "RA"}, {"family": "Ritvos", "given": "Olli", "initials": "O"}, {"family": "\u00d6sterlund", "given": "Pamela", "initials": "P", "orcid": "0000-0002-2229-6661", "researcher": {"href": "https://publications.scilifelab.se/researcher/f4657c21b1c1489eb139604a276b8738.json"}}, {"family": "Sironen", "given": "Tarja", "initials": "T", "orcid": "0000-0002-2344-2755", "researcher": {"href": "https://publications.scilifelab.se/researcher/8060fb77390f429e862149edf1435b5c.json"}}, {"family": "Vapalahti", "given": "Olli", "initials": "O", "orcid": "0000-0003-2270-6824", "researcher": {"href": "https://publications.scilifelab.se/researcher/ddd242b27ab54495aa9e135590938eaf.json"}}, {"family": "Kipar", "given": "Anja", "initials": "A", "orcid": "0000-0001-7289-3459", "researcher": {"href": "https://publications.scilifelab.se/researcher/0b82e8542b104dd79e189137733305e1.json"}}, {"family": "Huiskonen", "given": "Juha T", "initials": "JT", "orcid": "0000-0002-0348-7323", "researcher": {"href": "https://publications.scilifelab.se/researcher/c858a2e60971462f9e53653320c9eb0f.json"}}, {"family": "Rissanen", "given": "Ilona", "initials": "I", "orcid": "0000-0003-4937-1825", "researcher": {"href": "https://publications.scilifelab.se/researcher/20befd1e39cc4530b46c4f961c9a435c.json"}}, {"family": "Saksela", "given": "Kalle", "initials": "K", "orcid": "0000-0003-0827-122X", "researcher": {"href": "https://publications.scilifelab.se/researcher/5615e2d363d84510bd0e70b80f886f5f.json"}}], "type": "journal article", "published": "2023-03-24", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "14", "issue": "1", "pages": "1637", "issn-l": "2041-1723"}, "abstract": "The emergence of increasingly immunoevasive SARS-CoV-2 variants emphasizes the need for prophylactic strategies to complement vaccination in fighting the COVID-19 pandemic. Intranasal administration of neutralizing antibodies has shown encouraging protective potential but there remains a need for SARS-CoV-2 blocking agents that are less vulnerable to mutational viral variation and more economical to produce in large scale. Here we describe TriSb92, a highly manufacturable and stable trimeric antibody-mimetic sherpabody targeted against a conserved region of the viral spike glycoprotein. TriSb92 potently neutralizes SARS-CoV-2, including the latest Omicron variants like BF.7, XBB, and BQ.1.1. In female Balb/c mice intranasal administration of just 5 or 50 micrograms of TriSb92 as early as 8 h before but also 4 h after SARS-CoV-2 challenge can protect from infection. Cryo-EM and biochemical studies reveal triggering of a conformational shift in the spike trimer as the inhibitory mechanism of TriSb92. The potency and robust biochemical properties of TriSb92 together with its resistance against viral sequence evolution suggest that TriSb92 could be useful as a nasal spray for protecting susceptible individuals from SARS-CoV-2 infection.", "doi": "10.1038/s41467-023-37290-6", "pmid": "36964125", "labels": {"Cryo-EM": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC10037368"}, {"db": "pii", "key": "10.1038/s41467-023-37290-6"}], "notes": [], "created": "2023-06-08T20:24:47.138Z", "modified": "2023-11-26T14:06:35.516Z"}, {"entity": "publication", "iuid": "1350e5c702b3487dba34a7e5cf586808", "links": {"self": {"href": "https://publications.scilifelab.se/publication/1350e5c702b3487dba34a7e5cf586808.json"}, "display": {"href": "https://publications.scilifelab.se/publication/1350e5c702b3487dba34a7e5cf586808"}}, "title": "Nanobody engineering for SARS-CoV-2 neutralization and detection", "authors": [{"family": "Hannula", "given": "Liina", "initials": "L", "orcid": "0000-0001-7586-7539", "researcher": {"href": "https://publications.scilifelab.se/researcher/0c5c0e90fd5e4247bbd03f2beac0d955.json"}}, {"family": "Kuivanen", "given": "Suvi", "initials": "S", "orcid": "0000-0002-2347-5397", "researcher": {"href": "https://publications.scilifelab.se/researcher/ff9ce113016a4ea7a81cebd045ae7547.json"}}, {"family": "Lasham", "given": "Jonathan", "initials": "J", "orcid": "0000-0001-6393-9654", "researcher": {"href": "https://publications.scilifelab.se/researcher/47da5df97f3f4bea8eab630eb26b36d4.json"}}, {"family": "Kant", "given": "Ravi", "initials": "R", "orcid": "0000-0003-3878-9775", "researcher": {"href": "https://publications.scilifelab.se/researcher/5ee44aebd7174ab9bb5fc741c0a13e2b.json"}}, {"family": "Kareinen", "given": "Lauri", "initials": "L", "orcid": "0000-0003-2525-7817", "researcher": {"href": "https://publications.scilifelab.se/researcher/57a587b74c2d43e18ea4d580125bb049.json"}}, {"family": "Bogacheva", "given": "Mariia", "initials": "M", "orcid": "0000-0001-6608-1120", "researcher": {"href": "https://publications.scilifelab.se/researcher/4fbc1f8cb4af43baac9dbc587b34950a.json"}}, {"family": "Strandin", "given": "Tomas", "initials": "T", "orcid": "0000-0002-2454-7479", "researcher": {"href": "https://publications.scilifelab.se/researcher/fcfa4fe443d04b258f6b45ec84297565.json"}}, {"family": "Sironen", "given": "Tarja", "initials": "T", "orcid": "0000-0002-2344-2755", "researcher": {"href": "https://publications.scilifelab.se/researcher/8060fb77390f429e862149edf1435b5c.json"}}, {"family": "Sharma", "given": "Vivek", "initials": "V", "orcid": "0000-0002-8838-3151", "researcher": {"href": "https://publications.scilifelab.se/researcher/5a6299ddcafc4274940548261eddd42e.json"}}, {"family": "Saviranta", "given": "Petri", "initials": "P", "orcid": "0000-0002-3289-0621", "researcher": {"href": "https://publications.scilifelab.se/researcher/4b23027247eb47f7a62dd0e6eb460b33.json"}}, {"family": "Kipar", "given": "Anja", "initials": "A"}, {"family": "Vapalahti", "given": "Olli", "initials": "O", "orcid": "0000-0003-2270-6824", "researcher": {"href": "https://publications.scilifelab.se/researcher/ddd242b27ab54495aa9e135590938eaf.json"}}, {"family": "Huiskonen", "given": "Juha T", "initials": "JT", "orcid": "0000-0002-0348-7323", "researcher": {"href": "https://publications.scilifelab.se/researcher/c858a2e60971462f9e53653320c9eb0f.json"}}, {"family": "Rissanen", "given": "Ilona", "initials": "I", "orcid": "0000-0003-4937-1825", "researcher": {"href": "https://publications.scilifelab.se/researcher/20befd1e39cc4530b46c4f961c9a435c.json"}}], "type": "posted-content", "published": "2022-09-14", "journal": {"title": "biorxiv", "issn": null, "issn-l": null, "volume": null, "issue": null, "pages": null}, "abstract": null, "doi": "10.1101/2022.09.14.507920", "pmid": null, "labels": {"Cryo-EM": "Service"}, "xrefs": [], "notes": [], "created": "2023-11-26T14:09:02.711Z", "modified": "2025-12-18T19:59:56.976Z"}, {"entity": "publication", "iuid": "2034709ecac646fe9f1ac1a71faa2c00", "links": {"self": {"href": "https://publications.scilifelab.se/publication/2034709ecac646fe9f1ac1a71faa2c00.json"}, "display": {"href": "https://publications.scilifelab.se/publication/2034709ecac646fe9f1ac1a71faa2c00"}}, "title": "Structural basis of rapid actin dynamics in the evolutionarily divergent Leishmania parasite.", "authors": [{"family": "Kotila", "given": "Tommi", "initials": "T", "orcid": "0000-0002-9046-5834", "researcher": {"href": "https://publications.scilifelab.se/researcher/d530363e23ac4e3e8e24e7cdecfd0fe5.json"}}, {"family": "Wioland", "given": "Hugo", "initials": "H", "orcid": "0000-0001-5254-9642", "researcher": {"href": "https://publications.scilifelab.se/researcher/578f075141c245deb2f4c08702ef5090.json"}}, {"family": "Selvaraj", "given": "Muniyandi", "initials": "M", "orcid": "0000-0001-7793-6323", "researcher": {"href": "https://publications.scilifelab.se/researcher/1e74a48173fa4365897dd74ab6143c97.json"}}, {"family": "Kogan", "given": "Konstantin", "initials": "K", "orcid": "0000-0003-3270-9391", "researcher": {"href": "https://publications.scilifelab.se/researcher/1c6ce8d9a1474d5b9532650d2f5014d0.json"}}, {"family": "Antenucci", "given": "Lina", "initials": "L"}, {"family": "J\u00e9gou", "given": "Antoine", "initials": "A", "orcid": "0000-0003-0356-3127", "researcher": {"href": "https://publications.scilifelab.se/researcher/4e53291bfb224bfcb856be6e8ad06d9d.json"}}, {"family": "Huiskonen", "given": "Juha T", "initials": "JT", "orcid": "0000-0002-0348-7323", "researcher": {"href": "https://publications.scilifelab.se/researcher/c858a2e60971462f9e53653320c9eb0f.json"}}, {"family": "Romet-Lemonne", "given": "Guillaume", "initials": "G"}, {"family": "Lappalainen", "given": "Pekka", "initials": "P", "orcid": "0000-0001-6227-0354", "researcher": {"href": "https://publications.scilifelab.se/researcher/f9125bfd659d4e0784ce349140f84f5f.json"}}], "type": "journal article", "published": "2022-06-15", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "13", "issue": "1", "pages": "3442", "issn-l": "2041-1723"}, "abstract": "Actin polymerization generates forces for cellular processes throughout the eukaryotic kingdom, but our understanding of the 'ancient' actin turnover machineries is limited. We show that, despite > 1 billion years of evolution, pathogenic Leishmania major parasite and mammalian actins share the same overall fold and co-polymerize with each other. Interestingly, Leishmania harbors a simple actin-regulatory machinery that lacks cofilin 'cofactors', which accelerate filament disassembly in higher eukaryotes. By applying single-filament biochemistry we discovered that, compared to mammalian proteins, Leishmania actin filaments depolymerize more rapidly from both ends, and are severed > 100-fold more efficiently by cofilin. Our high-resolution cryo-EM structures of Leishmania ADP-, ADP-Pi- and cofilin-actin filaments identify specific features at actin subunit interfaces and cofilin-actin interactions that explain the unusually rapid dynamics of parasite actin filaments. Our findings reveal how divergent parasites achieve rapid actin dynamics using a remarkably simple set of actin-binding proteins, and elucidate evolution of the actin cytoskeleton.", "doi": "10.1038/s41467-022-31068-y", "pmid": "35705539", "labels": {"Cryo-EM": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC9200798"}, {"db": "pii", "key": "10.1038/s41467-022-31068-y"}], "notes": [], "created": "2022-11-30T09:31:12.829Z", "modified": "2023-11-26T14:07:44.750Z"}, {"entity": "publication", "iuid": "32532dfcd911424f93ddea636563394f", "links": {"self": {"href": "https://publications.scilifelab.se/publication/32532dfcd911424f93ddea636563394f.json"}, "display": {"href": "https://publications.scilifelab.se/publication/32532dfcd911424f93ddea636563394f"}}, "title": "Snapshots of actin and tubulin folding inside the TRiC chaperonin.", "authors": [{"family": "Kelly", "given": "John J", "initials": "JJ"}, {"family": "Tranter", "given": "Dale", "initials": "D"}, {"family": "Pardon", "given": "Els", "initials": "E", "orcid": "0000-0002-2466-0172", "researcher": {"href": "https://publications.scilifelab.se/researcher/375719a54c844150a4d5cb2b4f3ebb57.json"}}, {"family": "Chi", "given": "Gamma", "initials": "G"}, {"family": "Kramer", "given": "Holger", "initials": "H", "orcid": "0000-0002-6400-0945", "researcher": {"href": "https://publications.scilifelab.se/researcher/cf9fb27444604e999fe8815be9eb336a.json"}}, {"family": "Happonen", "given": "Lotta", "initials": "L", "orcid": "0000-0002-5922-4549", "researcher": {"href": "https://publications.scilifelab.se/researcher/a7af0a6faf6a48e7937a644be472edbe.json"}}, {"family": "Knee", "given": "Kelly M", "initials": "KM"}, {"family": "Janz", "given": "Jay M", "initials": "JM"}, {"family": "Steyaert", "given": "Jan", "initials": "J", "orcid": "0000-0002-3825-874X", "researcher": {"href": "https://publications.scilifelab.se/researcher/2a13eb5511e1448fb982b5bd1bb1d6f1.json"}}, {"family": "Bulawa", "given": "Christine", "initials": "C"}, {"family": "Paavilainen", "given": "Ville O", "initials": "VO", "orcid": "0000-0002-3160-7767", "researcher": {"href": "https://publications.scilifelab.se/researcher/f76359e8b92b4b53af80c36d064a1a42.json"}}, {"family": "Huiskonen", "given": "Juha T", "initials": "JT", "orcid": "0000-0002-0348-7323", "researcher": {"href": "https://publications.scilifelab.se/researcher/c858a2e60971462f9e53653320c9eb0f.json"}}, {"family": "Yue", "given": "Wyatt W", "initials": "WW", "orcid": "0000-0001-6959-6007", "researcher": {"href": "https://publications.scilifelab.se/researcher/5be92e7076054a009efe9d23f78b5a48.json"}}], "type": "journal article", "published": "2022-04-21", "journal": {"title": "Nat. Struct. Mol. Biol.", "issn": "1545-9985", "issn-l": "1545-9985"}, "abstract": "The integrity of a cell's proteome depends on correct folding of polypeptides by chaperonins. The chaperonin TCP-1 ring complex (TRiC) acts as obligate folder for >10% of cytosolic proteins, including he cytoskeletal proteins actin and tubulin. Although its architecture and how it recognizes folding substrates are emerging from structural studies, the subsequent fate of substrates inside the TRiC chamber is not defined. We trapped endogenous human TRiC with substrates (actin, tubulin) and cochaperone (PhLP2A) at different folding stages, for structure determination by cryo-EM. The already-folded regions of client proteins are anchored at the chamber wall, positioning unstructured regions toward the central space to achieve their native fold. Substrates engage with different sections of the chamber during the folding cycle, coupled to TRiC open-and-close transitions. Further, the cochaperone PhLP2A modulates folding, acting as a molecular strut between substrate and TRiC chamber. Our structural snapshots piece together an emerging model of client protein folding within TRiC.", "doi": "10.1038/s41594-022-00755-1", "pmid": "35449234", "labels": {"Structural Proteomics": "Collaborative"}, "xrefs": [{"db": "pii", "key": "10.1038/s41594-022-00755-1"}], "notes": [], "created": "2022-04-27T14:22:02.454Z", "modified": "2022-04-27T14:22:02.677Z"}]}