{"entity": "researcher", "timestamp": "2026-03-05T07:50:43.991Z", "family": "Boerjan", "given": "Wout", "initials": "W", "orcid": "0000-0003-1495-510X", "affiliations": ["Department of Plant Biotechnology and Bioinformatics Ghent University Technologiepark 71 9052 Ghent Belgium", "VIB Center for Plant Systems Biology Technologiepark 71 9052 Ghent Belgium"], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/c8215f848a3047669e8896145e8db4b5.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/c8215f848a3047669e8896145e8db4b5"}}, "publications": [{"entity": "publication", "iuid": "ab5a9cefcf044daba4246362a58f1356", "links": {"self": {"href": "https://publications.scilifelab.se/publication/ab5a9cefcf044daba4246362a58f1356.json"}, "display": {"href": "https://publications.scilifelab.se/publication/ab5a9cefcf044daba4246362a58f1356"}}, "title": "PIRIN2 suppresses S-type lignin accumulation in a noncell-autonomous manner in Arabidopsis xylem elements.", "authors": [{"family": "Zhang", "given": "Bo", "initials": "B"}, {"family": "Sztojka", "given": "Bernadette", "initials": "B"}, {"family": "Escamez", "given": "Sacha", "initials": "S"}, {"family": "Vanholme", "given": "Ruben", "initials": "R"}, {"family": "Hedenstr\u00f6m", "given": "Mattias", "initials": "M"}, {"family": "Wang", "given": "Yin", "initials": "Y"}, {"family": "Turumtay", "given": "Halbay", "initials": "H"}, {"family": "Gorzs\u00e1s", "given": "Andr\u00e1s", "initials": "A", "orcid": "0000-0002-2298-8844", "researcher": {"href": "https://publications.scilifelab.se/researcher/8070792ccead4c809c89943d84cf0e03.json"}}, {"family": "Boerjan", "given": "Wout", "initials": "W", "orcid": "0000-0003-1495-510X", "researcher": {"href": "https://publications.scilifelab.se/researcher/c8215f848a3047669e8896145e8db4b5.json"}}, {"family": "Tuominen", "given": "Hannele", "initials": "H", "orcid": "0000-0002-4949-3702", "researcher": {"href": "https://publications.scilifelab.se/researcher/0fe575e1cb054ab08b57e05d3a1ee28d.json"}}], "type": "journal article", "published": "2020-03-00", "journal": {"title": "New Phytol.", "issn": "1469-8137", "volume": "225", "issue": "5", "pages": "1923-1935", "issn-l": "0028-646X"}, "abstract": "PIRIN (PRN) genes encode cupin domain-containing proteins that function as transcriptional co-regulators in humans but that are poorly described in plants. A previous study in xylogenic cell cultures of Zinnia elegans suggested a role for a PRN protein in lignification. This study aimed to identify the function of Arabidopsis (Arabidopsis thaliana) PRN proteins in lignification of xylem tissues. Chemical composition of the secondary cell walls was analysed in Arabidopsis stems and/or hypocotyls by pyrolysis-gas chromatography/mass spectrometry, 2D-nuclear magnetic resonance and phenolic profiling. Secondary cell walls of individual xylem elements were chemotyped by Fourier transform infrared and Raman microspectroscopy. Arabidopsis PRN2 suppressed accumulation of S-type lignin in Arabidopsis stems and hypocotyls. PRN2 promoter activity and PRN2:GFP fusion protein were localised specifically in cells next to the vessel elements, suggesting a role for PRN2 in noncell-autonomous lignification of xylem vessels. Accordingly, PRN2 modulated lignin chemistry in the secondary cell walls of the neighbouring vessel elements. These results indicate that PRN2 suppresses S-type lignin accumulation in the neighbourhood of xylem vessels to bestow G-type enriched lignin composition on the secondary cell walls of the vessel elements. Gene expression analyses suggested that PRN2 function is mediated by regulation of the expression of the lignin-biosynthetic genes.", "doi": "10.1111/nph.16271", "pmid": "31625609", "labels": {"Swedish NMR Centre": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC7027918"}, {"db": "GENBANK", "key": "At3g59220"}, {"db": "GENBANK", "key": "At2g43120"}, {"db": "GENBANK", "key": "At3g59260"}, {"db": "GENBANK", "key": "At1g50590"}, {"db": "GENBANK", "key": "At2g37040"}, {"db": "GENBANK", "key": "At2g30490"}, {"db": "GENBANK", "key": "At1g51680"}, {"db": "GENBANK", "key": "At2g40890"}, {"db": "GENBANK", "key": "At5g48930"}, {"db": "GENBANK", "key": "At4g34050"}, {"db": "GENBANK", "key": "At1g15950"}, {"db": "GENBANK", "key": "At4g36220"}, {"db": "GENBANK", "key": "At5g54160"}, {"db": "GENBANK", "key": "At4g34230"}, {"db": "GENBANK", "key": "At5g62380"}, {"db": "GENBANK", "key": "At1g71930"}, {"db": "GENBANK", "key": "At2g46770"}, {"db": "GENBANK", "key": "At3g61910"}, {"db": "GENBANK", "key": "At1g32770"}, {"db": "GENBANK", "key": "At5g12870"}, {"db": "GENBANK", "key": "At3g08500"}, {"db": "GENBANK", "key": "At1g16490"}, {"db": "GENBANK", "key": "At1g79180"}, {"db": "GENBANK", "key": "At4g22680"}, {"db": "GENBANK", "key": "At1g09540"}, {"db": "GENBANK", "key": "At1g63910"}], "notes": [], "created": "2019-11-22T14:28:02.623Z", "modified": "2025-10-17T13:03:57.081Z"}, {"entity": "publication", "iuid": "bffdc421008341a591e5c8e562205455", "links": {"self": {"href": "https://publications.scilifelab.se/publication/bffdc421008341a591e5c8e562205455.json"}, "display": {"href": "https://publications.scilifelab.se/publication/bffdc421008341a591e5c8e562205455"}}, "title": "The Allelochemical MDCA Inhibits Lignification and Affects Auxin Homeostasis.", "authors": [{"family": "Steenackers", "given": "Ward", "initials": "W"}, {"family": "Cesarino", "given": "Igor", "initials": "I", "orcid": "0000-0002-6789-2432", "researcher": {"href": "https://publications.scilifelab.se/researcher/cdb44fbb92af48ff92d2854f4bd95a3b.json"}}, {"family": "Kl\u00edma", "given": "Petr", "initials": "P", "orcid": "0000-0001-5678-2919", "researcher": {"href": "https://publications.scilifelab.se/researcher/449675d45ea64435a73a60bdf76d3d20.json"}}, {"family": "Quareshy", "given": "Mussa", "initials": "M", "orcid": "0000-0001-8115-9803", "researcher": {"href": "https://publications.scilifelab.se/researcher/8b7ecbb5893d4c1ba2d96087ff89cf70.json"}}, {"family": "Vanholme", "given": "Ruben", "initials": "R", "orcid": "0000-0001-5848-3138", "researcher": {"href": "https://publications.scilifelab.se/researcher/ab4700dc00c84477b42b04f800342121.json"}}, {"family": "Corneillie", "given": "Sander", "initials": "S"}, {"family": "Kumpf", "given": "Robert Peter", "initials": "RP", "orcid": "0000-0002-4046-6935", "researcher": {"href": "https://publications.scilifelab.se/researcher/89a9f19855fe4a719fb69cc7b613764d.json"}}, {"family": "Van de Wouwer", "given": "Dorien", "initials": "D"}, {"family": "Ljung", "given": "Karin", "initials": "K", "orcid": "0000-0003-2901-189X", "researcher": {"href": "https://publications.scilifelab.se/researcher/f91b1e1f90c24559b915ebcd265804a4.json"}}, {"family": "Goeminne", "given": "Geert", "initials": "G", "orcid": "0000-0002-0337-2999", "researcher": {"href": "https://publications.scilifelab.se/researcher/de05b07d3b044577a9e606d19c3004fd.json"}}, {"family": "Nov\u00e1k", "given": "Ond\u0159ej", "initials": "O", "orcid": "0000-0003-3452-0154", "researcher": {"href": "https://publications.scilifelab.se/researcher/8c19165acb9a4ff79dd96af7fccdc5f8.json"}}, {"family": "Za\u017e\u00edmalov\u00e1", "given": "Eva", "initials": "E"}, {"family": "Napier", "given": "Richard", "initials": "R", "orcid": "0000-0002-0605-518X", "researcher": {"href": "https://publications.scilifelab.se/researcher/45b807545a9f46ad90011b850dfb1328.json"}}, {"family": "Boerjan", "given": "Wout", "initials": "W", "orcid": "0000-0003-1495-510X", "researcher": {"href": "https://publications.scilifelab.se/researcher/c8215f848a3047669e8896145e8db4b5.json"}}, {"family": "Vanholme", "given": "Bartel", "initials": "B", "orcid": "0000-0002-7214-7170", "researcher": {"href": "https://publications.scilifelab.se/researcher/ef6569a7a1814aa5acd9099d26e4b786.json"}}], "type": "journal article", "published": "2016-10-00", "journal": {"title": "Plant Physiol.", "issn": "1532-2548", "volume": "172", "issue": "2", "pages": "874-888", "issn-l": "0032-0889"}, "abstract": "The phenylpropanoid 3,4-(methylenedioxy)cinnamic acid (MDCA) is a plant-derived compound first extracted from roots of Asparagus officinalis and further characterized as an allelochemical. Later on, MDCA was identified as an efficient inhibitor of 4-COUMARATE-CoA LIGASE (4CL), a key enzyme of the general phenylpropanoid pathway. By blocking 4CL, MDCA affects the biosynthesis of many important metabolites, which might explain its phytotoxicity. To decipher the molecular basis of the allelochemical activity of MDCA, we evaluated the effect of this compound on Arabidopsis thaliana seedlings. Metabolic profiling revealed that MDCA is converted in planta into piperonylic acid (PA), an inhibitor of CINNAMATE-4-HYDROXYLASE (C4H), the enzyme directly upstream of 4CL. The inhibition of C4H was also reflected in the phenolic profile of MDCA-treated plants. Treatment of in vitro grown plants resulted in an inhibition of primary root growth and a proliferation of lateral and adventitious roots. These observed growth defects were not the consequence of lignin perturbation, but rather the result of disturbing auxin homeostasis. Based on DII-VENUS quantification and direct measurement of cellular auxin transport, we concluded that MDCA disturbs auxin gradients by interfering with auxin efflux. In addition, mass spectrometry was used to show that MDCA triggers auxin biosynthesis, conjugation, and catabolism. A similar shift in auxin homeostasis was found in the c4h mutant ref3-2, indicating that MDCA triggers a cross talk between the phenylpropanoid and auxin biosynthetic pathways independent from the observed auxin efflux inhibition. Altogether, our data provide, to our knowledge, a novel molecular explanation for the phytotoxic properties of MDCA.", "doi": "10.1104/pp.15.01972", "pmid": "27506238", "labels": {"Swedish Metabolomics Centre": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC5047068"}, {"db": "pii", "key": "pp.15.01972"}], "notes": [], "created": "2023-04-12T14:14:52.688Z", "modified": "2025-10-17T13:03:19.381Z"}]}