{"entity": "researcher", "timestamp": "2026-07-20T13:24:27.602Z", "family": "Dimberg", "given": "Anna", "initials": "A", "orcid": "0000-0003-4422-9125", "affiliations": ["Department of Immunology, Genetics and Pathology, Uppsala University, Rudbeck Laboratory, Uppsala, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/c53166298a214331866c8cbf3bb9a3b9.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/c53166298a214331866c8cbf3bb9a3b9"}}, "publications": [{"entity": "publication", "iuid": "f09706baf2a74636a49097f82b60b681", "links": {"self": {"href": "https://publications.scilifelab.se/publication/f09706baf2a74636a49097f82b60b681.json"}, "display": {"href": "https://publications.scilifelab.se/publication/f09706baf2a74636a49097f82b60b681"}}, "title": "VLDLR mediates Semliki Forest virus neuroinvasion through the blood-cerebrospinal fluid barrier.", "authors": [{"family": "Martikainen", "given": "Miika", "initials": "M", "orcid": "0000-0001-8202-701X", "researcher": {"href": "https://publications.scilifelab.se/researcher/3477399dd7474fc6a18863b4c239d81c.json"}}, {"family": "Lugano", "given": "Roberta", "initials": "R"}, {"family": "Pietil\u00e4", "given": "Ilkka", "initials": "I"}, {"family": "Brosch", "given": "Sofie", "initials": "S"}, {"family": "Cabrolier", "given": "Camille", "initials": "C"}, {"family": "Sivaramakrishnan", "given": "Aishwarya", "initials": "A"}, {"family": "Ramachandran", "given": "Mohanraj", "initials": "M", "orcid": "0000-0003-2685-0575", "researcher": {"href": "https://publications.scilifelab.se/researcher/f3fefc78a0b040faa998efb8fde7b920.json"}}, {"family": "Yu", "given": "Di", "initials": "D", "orcid": "0000-0002-8636-0351", "researcher": {"href": "https://publications.scilifelab.se/researcher/cfc1c71920c74292b4392a2ee108998d.json"}}, {"family": "Dimberg", "given": "Anna", "initials": "A", "orcid": "0000-0003-4422-9125", "researcher": {"href": "https://publications.scilifelab.se/researcher/c53166298a214331866c8cbf3bb9a3b9.json"}}, {"family": "Essand", "given": "Magnus", "initials": "M", "orcid": "0000-0002-9725-0422", "researcher": {"href": "https://publications.scilifelab.se/researcher/c5afc47ab0814c09909af3c66217a3a6.json"}}], "type": "journal article", "published": "2024-12-23", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "15", "issue": "1", "pages": "10718", "issn-l": "2041-1723"}, "abstract": "Semliki Forest virus (SFV) is a neuropathogenic alphavirus which is of interest both as a model neurotropic alphavirus and as an oncolytic virus with proven potency in preclinical cancer models. In laboratory mice, peripherally administered SFV infiltrates the central nervous system (CNS) and causes encephalitis of varying severity. The route of SFV CNS entrance is poorly understood but has been considered to occur through the blood-brain barrier. Here we show that neuroinvasion of intravenously administered SFV is strictly dependent on very-low-density-lipoprotein receptor (VLDLR) which acts as an entry receptor for SFV. Moreover, SFV primarily enters the CNS through the blood-cerebrospinal fluid (B-CSF) barrier via infecting choroid plexus epithelial cells which show distinctly high expression of VLDLR. This is the first indication of neurotropic alphavirus utilizing choroid plexus for CNS entry, and VLDLR playing a specific and crucial role for mediating SFV entry through this pathway.", "doi": "10.1038/s41467-024-55493-3", "pmid": "39715740", "labels": {"Bioinformatics Support for Computational Resources": "Service", "CRISPR Functional Genomics": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11666578"}, {"db": "pii", "key": "10.1038/s41467-024-55493-3"}, {"db": "GEO", "key": "GSE283607"}], "notes": [], "created": "2025-02-28T14:14:51.638Z", "modified": "2025-09-15T09:21:34.660Z"}, {"entity": "publication", "iuid": "0f4b2f133342404c882907cf77fe61be", "links": {"self": {"href": "https://publications.scilifelab.se/publication/0f4b2f133342404c882907cf77fe61be.json"}, "display": {"href": "https://publications.scilifelab.se/publication/0f4b2f133342404c882907cf77fe61be"}}, "title": "Complementarity-determining region clustering may cause CAR-T cell dysfunction.", "authors": [{"family": "Sar\u00e9n", "given": "Tina", "initials": "T", "orcid": "0000-0001-5227-6779", "researcher": {"href": "https://publications.scilifelab.se/researcher/b8f06b113565445f8dc2882a926229b6.json"}}, {"family": "Saronio", "given": "Giulia", "initials": "G"}, {"family": "Marti Torrell", "given": "Paula", "initials": "P"}, {"family": "Zhu", "given": "Xu", "initials": "X"}, {"family": "Thelander", "given": "Josefin", "initials": "J"}, {"family": "Andersson", "given": "Yasmin", "initials": "Y"}, {"family": "Hofstr\u00f6m", "given": "Camilla", "initials": "C"}, {"family": "Nestor", "given": "Marika", "initials": "M"}, {"family": "Dimberg", "given": "Anna", "initials": "A", "orcid": "0000-0003-4422-9125", "researcher": {"href": "https://publications.scilifelab.se/researcher/c53166298a214331866c8cbf3bb9a3b9.json"}}, {"family": "Persson", "given": "Helena", "initials": "H"}, {"family": "Ramachandran", "given": "Mohanraj", "initials": "M", "orcid": "0000-0003-2685-0575", "researcher": {"href": "https://publications.scilifelab.se/researcher/f3fefc78a0b040faa998efb8fde7b920.json"}}, {"family": "Yu", "given": "Di", "initials": "D", "orcid": "0000-0002-8636-0351", "researcher": {"href": "https://publications.scilifelab.se/researcher/cfc1c71920c74292b4392a2ee108998d.json"}}, {"family": "Essand", "given": "Magnus", "initials": "M", "orcid": "0000-0002-9725-0422", "researcher": {"href": "https://publications.scilifelab.se/researcher/c5afc47ab0814c09909af3c66217a3a6.json"}}], "type": "journal article", "published": "2023-08-10", "journal": {"title": "Nat Commun", "issn": "2041-1723", "issn-l": "2041-1723", "volume": "14", "issue": "1", "pages": "4732"}, "abstract": "Chimeric antigen receptor (CAR)-T cell therapy is rapidly advancing as cancer treatment, however, designing an optimal CAR remains challenging. A single-chain variable fragment (scFv) is generally used as CAR targeting moiety, wherein the complementarity-determining regions (CDRs) define its specificity. We report here that the CDR loops can cause CAR clustering, leading to antigen-independent tonic signalling and subsequent CAR-T cell dysfunction. We show via CARs incorporating scFvs with identical framework and varying CDR sequences that CARs may cluster on the T cell surface, which leads to antigen-independent CAR-T cell activation, characterized by increased cell size and interferon (IFN)-\u03b3 secretion. This results in CAR-T cell exhaustion, activation-induced cell death and reduced responsiveness to target-antigen-expressing tumour cells. CDR mutagenesis confirms that the CAR-clustering is mediated by CDR-loops. In summary, antigen-independent tonic signalling can be induced by CDR-mediated CAR clustering, which could not be predicted from the scFv sequences, but could be tested for by evaluating the activity of unstimulated CAR-T cells.", "doi": "10.1038/s41467-023-40303-z", "pmid": "37563127", "labels": {"Global Proteomics and Proteogenomics": "Service", "Bioinformatics Support for Computational Resources": "Service", "Drug Discovery and Development": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC10415375"}, {"db": "pii", "key": "10.1038/s41467-023-40303-z"}], "notes": [], "created": "2023-08-14T06:46:42.174Z", "modified": "2025-10-17T13:05:07.543Z"}, {"entity": "publication", "iuid": "058e4ab4248b4f38bf463af3cb986da6", "links": {"self": {"href": "https://publications.scilifelab.se/publication/058e4ab4248b4f38bf463af3cb986da6.json"}, "display": {"href": "https://publications.scilifelab.se/publication/058e4ab4248b4f38bf463af3cb986da6"}}, "title": "A method for Boolean analysis of protein interactions at a molecular level.", "authors": [{"family": "Raykova", "given": "Doroteya", "initials": "D", "orcid": "0000-0001-6452-2199", "researcher": {"href": "https://publications.scilifelab.se/researcher/0a81c40491e349178167f148f2351875.json"}}, {"family": "Kermpatsou", "given": "Despoina", "initials": "D", "orcid": "0000-0001-5872-4472", "researcher": {"href": "https://publications.scilifelab.se/researcher/cfafe1fe67374c25ac5111ecd42c2fd4.json"}}, {"family": "Malmqvist", "given": "Tony", "initials": "T", "orcid": "0000-0003-0609-2009", "researcher": {"href": "https://publications.scilifelab.se/researcher/ee835b51354e4cb5aff5dfbf43964dc8.json"}}, {"family": "Harrison", "given": "Philip J", "initials": "PJ"}, {"family": "Sander", "given": "Marie Rubin", "initials": "MR", "orcid": "0000-0002-9783-5682", "researcher": {"href": "https://publications.scilifelab.se/researcher/68a774625f6948d693bf4ad09eaba273.json"}}, {"family": "Stiller", "given": "Christiane", "initials": "C", "orcid": "0000-0002-6552-8426", "researcher": {"href": "https://publications.scilifelab.se/researcher/2f8bb525e1094fbfbfb71dbbff62cbc7.json"}}, {"family": "Heldin", "given": "Johan", "initials": "J", "orcid": "0000-0002-0915-5303", "researcher": {"href": "https://publications.scilifelab.se/researcher/d8a546798d014cd3a44537ae5db9f889.json"}}, {"family": "Leino", "given": "Mattias", "initials": "M"}, {"family": "Ricardo", "given": "Sara", "initials": "S", "orcid": "0000-0003-4091-2226", "researcher": {"href": "https://publications.scilifelab.se/researcher/d5599dd22d9e44a5af16850baf14e10e.json"}}, {"family": "Klemm", "given": "Anna", "initials": "A", "orcid": "0000-0002-3466-1320", "researcher": {"href": "https://publications.scilifelab.se/researcher/4ae78afb2a424b0ab70d49871f361d13.json"}}, {"family": "David", "given": "Leonor", "initials": "L", "orcid": "0000-0003-4207-9258", "researcher": {"href": "https://publications.scilifelab.se/researcher/510030f8783044e8b644277783308bf6.json"}}, {"family": "Spjuth", "given": "Ola", "initials": "O", "orcid": "0000-0002-8083-2864", "researcher": {"href": "https://publications.scilifelab.se/researcher/605dbd52684d4e54ae4150a9933abe6e.json"}}, {"family": "Vemuri", "given": "Kalyani", "initials": "K", "orcid": "0000-0003-2544-5412", "researcher": {"href": "https://publications.scilifelab.se/researcher/b74c149757644aa5a0997af9ca583a45.json"}}, {"family": "Dimberg", "given": "Anna", "initials": "A", "orcid": "0000-0003-4422-9125", "researcher": {"href": "https://publications.scilifelab.se/researcher/c53166298a214331866c8cbf3bb9a3b9.json"}}, {"family": "Sundqvist", "given": "Anders", "initials": "A"}, {"family": "Norlin", "given": "Maria", "initials": "M", "orcid": "0000-0003-4348-6269", "researcher": {"href": "https://publications.scilifelab.se/researcher/a10ffe5af28b4398b8d19743f7927e34.json"}}, {"family": "Klaesson", "given": "Axel", "initials": "A"}, {"family": "Kampf", "given": "Caroline", "initials": "C"}, {"family": "S\u00f6derberg", "given": "Ola", "initials": "O", "orcid": "0000-0003-2883-1925", "researcher": {"href": "https://publications.scilifelab.se/researcher/68df823efa304c0b9962684ac1515808.json"}}], "type": "journal article", "published": "2022-08-13", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "13", "issue": "1", "pages": "4755", "issn-l": "2041-1723"}, "abstract": "Determining the levels of protein-protein interactions is essential for the analysis of signaling within the cell, characterization of mutation effects, protein function and activation in health and disease, among others. Herein, we describe MolBoolean - a method to detect interactions between endogenous proteins in various subcellular compartments, utilizing antibody-DNA conjugates for identification and signal amplification. In contrast to proximity ligation assays, MolBoolean simultaneously indicates the relative abundances of protein A and B not interacting with each other, as well as the pool of A and B proteins that are proximal enough to be considered an AB complex. MolBoolean is applicable both in fixed cells and tissue sections. The specific and quantifiable data that the method generates provide opportunities for both diagnostic use and medical research.", "doi": "10.1038/s41467-022-32395-w", "pmid": "35963857", "labels": {"BioImage Informatics": "Collaborative", "Bioinformatics (NBIS)": "Collaborative"}, "xrefs": [{"db": "pii", "key": "10.1038/s41467-022-32395-w"}, {"db": "pmc", "key": "PMC9375095"}], "notes": [], "created": "2022-08-30T09:04:56.787Z", "modified": "2022-08-30T09:04:57.234Z"}]}