{"entity": "researcher", "timestamp": "2026-08-17T01:41:30.892Z", "family": "Angerer", "given": "Tina B", "initials": "TB", "orcid": "0000-0003-3852-6254", "affiliations": ["Department of Pharmaceutical Biosciences, Spatial Mass Spectrometry, Science for Life Laboratory, Uppsala University, Uppsala SE-75124, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/c3f5ed541c9f4e2bb6c7c7519d6ad36b.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/c3f5ed541c9f4e2bb6c7c7519d6ad36b"}}, "publications": [{"entity": "publication", "iuid": "0576cf154a2a400882560447fb8f649c", "links": {"self": {"href": "https://publications.scilifelab.se/publication/0576cf154a2a400882560447fb8f649c.json"}, "display": {"href": "https://publications.scilifelab.se/publication/0576cf154a2a400882560447fb8f649c"}}, "title": "Quantitative Mass Spectrometry Imaging Protocols for Spatially Heterogeneous Samples.", "authors": [{"family": "Shariatgorji", "given": "Reza", "initials": "R", "orcid": "0000-0001-9484-0921", "researcher": {"href": "https://publications.scilifelab.se/researcher/7762e9f6779c4780a4077c557eb7a3b6.json"}}, {"family": "Niehues", "given": "Michael", "initials": "M"}, {"family": "Nilsson", "given": "Anna", "initials": "A"}, {"family": "Angerer", "given": "Tina", "initials": "T", "orcid": "0000-0003-3852-6254", "researcher": {"href": "https://publications.scilifelab.se/researcher/c3f5ed541c9f4e2bb6c7c7519d6ad36b.json"}}, {"family": "Stroth", "given": "Nikolas", "initials": "N"}, {"family": "Paslawski", "given": "Wojciech", "initials": "W"}, {"family": "Jabre", "given": "Sandra", "initials": "S"}, {"family": "Svenningsson", "given": "Per", "initials": "P"}, {"family": "Andr\u00e9n", "given": "Per E", "initials": "PE", "orcid": "0000-0002-4062-7743", "researcher": {"href": "https://publications.scilifelab.se/researcher/64f6381de42949db8d30b56b526f3e26.json"}}], "type": "journal article", "published": "2025-06-03", "journal": {"title": "Anal. Chem.", "issn": "1520-6882", "volume": "97", "issue": "21", "pages": "10957-10961", "issn-l": "0003-2700"}, "abstract": "Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a promising tool for the spatial quantitation of endogenous and exogenous compounds directly in biological tissue sections. However, precise quantitation may be hampered due to matrix effects and variations in ionization efficiency, especially in spatially heterogeneous samples such as brain tissue. In this study, we developed and implemented two advanced MALDI-MSI protocols to address these limitations by employing a standard addition approach. The protocols involved the homogeneous spraying of standard solutions onto tissue sections to minimize the matrix effects associated with heterogeneous samples. The first method utilized spraying of deuterated analogues of neurotransmitters across all tissue sections for normalization, while calibration standards were applied in a quantitative manner to consecutive tissue sections. The second method employed two stable isotope-labeled compounds: one for calibration and the other for normalization. Both methods were applied to quantify neurotransmitters and their metabolites, e.g., dopamine, norepinephrine, and 3-methoxytyramine, in rodent brain tissue. The results showed strong linearity between signal intensities and analyte concentrations across brain tissue sections with values comparable to those obtained using high-performance liquid chromatography-electrochemical detection. The standard addition approach significantly enhanced the quantitation accuracy by accounting for tissue-specific matrix effects, providing a robust method for the spatial quantification of neurotransmitters in complex brain tissue environments.", "doi": "10.1021/acs.analchem.5c00677", "pmid": "40404577", "labels": {"Spatial Mass Spectrometry": "Technology development"}, "xrefs": [{"db": "pmc", "key": "PMC12138873"}], "notes": [], "created": "2025-11-21T09:59:52.122Z", "modified": "2025-11-21T09:59:52.198Z"}, {"entity": "publication", "iuid": "ad5916e84efd42529a1c95bb30f10292", "links": {"self": {"href": "https://publications.scilifelab.se/publication/ad5916e84efd42529a1c95bb30f10292.json"}, "display": {"href": "https://publications.scilifelab.se/publication/ad5916e84efd42529a1c95bb30f10292"}}, "title": "Applying Spatial Metabolomics To Investigate Age- and Drug-Induced Neurochemical Changes.", "authors": [{"family": "Vallianatou", "given": "Theodosia", "initials": "T", "orcid": "0000-0002-1477-7756", "researcher": {"href": "https://publications.scilifelab.se/researcher/ae610b7669754328a119654fdfcd6af4.json"}}, {"family": "Angerer", "given": "Tina B", "initials": "TB", "orcid": "0000-0003-3852-6254", "researcher": {"href": "https://publications.scilifelab.se/researcher/c3f5ed541c9f4e2bb6c7c7519d6ad36b.json"}}, {"family": "Kaya", "given": "Ibrahim", "initials": "I"}, {"family": "Nilsson", "given": "Anna", "initials": "A"}, {"family": "Shariatgorji", "given": "Reza", "initials": "R"}, {"family": "Svenningsson", "given": "Per", "initials": "P"}, {"family": "Andr\u00e9n", "given": "Per E", "initials": "PE", "orcid": "0000-0002-4062-7743", "researcher": {"href": "https://publications.scilifelab.se/researcher/64f6381de42949db8d30b56b526f3e26.json"}}], "type": "journal article", "published": "2024-08-07", "journal": {"title": "ACS Chem Neurosci", "issn": "1948-7193", "volume": "15", "issue": "15", "pages": "2822-2829", "issn-l": "1948-7193"}, "abstract": "In an era when population aging is increasing the burden of neurodegenerative conditions, deciphering the mechanisms underlying brain senescence is more important than ever. Here, we present a spatial metabolomics analysis of age-induced neurochemical alterations in the mouse brain using negative ionization mode mass spectrometry imaging. The age-dependent effects of the acetylcholinesterase inhibitor tacrine were simultaneously examined. For ultrahigh mass resolution analysis, we utilized a Fourier-transform ion cyclotron resonance spectrometer. To complement this, a trapped ion mobility spectrometry time-of-flight analyzer provided high speed and lateral resolution. The chosen approach facilitated the detection and identification of a wide range of metabolites, from amino acids to sphingolipids. We reported significant, age-dependent alterations in brain lipids which were most evident for sulfatides and lysophosphatidic acids. Sulfatide species, which are mainly localized to white matter, either increased or decreased with age, depending on the carbon chain length and hydroxylation stage. Lysophosphatidic acids were found to decrease with age in the detailed cortical and hippocampal subregions. An age-dependent increase in the glutamine/glutamate ratio, an indicator of glia-neuron interconnection and neurotoxicity, was detected after tacrine administration. The presented metabolic mapping approach was able to provide visualizations of the lipid signaling and neurotransmission alterations induced by early aging and can thus be beneficial to further elucidating age-related neurochemical pathways.", "doi": "10.1021/acschemneuro.4c00199", "pmid": "39072364", "labels": {"Spatial Mass Spectrometry": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC11311129"}], "notes": [], "created": "2024-11-20T10:41:26.891Z", "modified": "2024-11-20T10:41:27.370Z"}]}