{"entity": "researcher", "timestamp": "2026-07-15T17:05:01.609Z", "family": "Lanner", "given": "Fredrik", "initials": "F", "orcid": "0000-0002-2771-7445", "affiliations": ["Division of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden. Fredrik.Lanner@ki.se.", "Ming Wai Lau Center for Reparative Medicine, Stockholm node, Karolinska Institutet, Stockholm, Sweden. Fredrik.Lanner@ki.se."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/ba53ce48a35d413eb86cd104488ce669.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/ba53ce48a35d413eb86cd104488ce669"}}, "publications": [{"entity": "publication", "iuid": "9baaf85a6b744b00baac397292f47517", "links": {"self": {"href": "https://publications.scilifelab.se/publication/9baaf85a6b744b00baac397292f47517.json"}, "display": {"href": "https://publications.scilifelab.se/publication/9baaf85a6b744b00baac397292f47517"}}, "title": "A comprehensive human embryo reference tool using single-cell RNA-sequencing data", "authors": [{"family": "Zhao", "given": "Cheng", "initials": "C", "orcid": "0000-0003-0518-5924", "researcher": {"href": "https://publications.scilifelab.se/researcher/4cd6a95b29e44b3fa8fed63033d26393.json"}}, {"family": "Plaza Reyes", "given": "Alvaro", "initials": "A", "orcid": "0000-0003-1167-6316", "researcher": {"href": "https://publications.scilifelab.se/researcher/36f7ddaf16ac45bfad26c96f292f2072.json"}}, {"family": "Schell", "given": "John Paul", "initials": "JP"}, {"family": "Weltner", "given": "Jere", "initials": "J"}, {"family": "Ortega", "given": "Nicol\u00e1s M", "initials": "NM"}, {"family": "Zheng", "given": "Yi", "initials": "Y", "orcid": "0000-0002-2685-3680", "researcher": {"href": "https://publications.scilifelab.se/researcher/c5ae32c43903498dab060a3a31695705.json"}}, {"family": "Bj\u00f6rklund", "given": "\u00c5sa K", "initials": "\u00c5K", "orcid": "0000-0003-2224-7090", "researcher": {"href": "https://publications.scilifelab.se/researcher/8eb8c1fc5f704cbfb87471226485ae1f.json"}}, {"family": "Baqu\u00e9-Vidal", "given": "Laura", "initials": "L", "orcid": "0009-0008-8128-6069", "researcher": {"href": "https://publications.scilifelab.se/researcher/8cbb3bfdf71e4002acffa6b14ef58c60.json"}}, {"family": "Sokka", "given": "Joonas", "initials": "J", "orcid": "0000-0002-0377-8841", "researcher": {"href": "https://publications.scilifelab.se/researcher/f834bc634e2049539fb66b3286020ed4.json"}}, {"family": "Trokovic", "given": "Ras", "initials": "R", "orcid": "0000-0002-3065-6663", "researcher": {"href": "https://publications.scilifelab.se/researcher/510f22386f8744e2a50d7f803b3da831.json"}}, {"family": "Cox", "given": "Brian", "initials": "B"}, {"family": "Rossant", "given": "Janet", "initials": "J", "orcid": "0000-0002-3731-5466", "researcher": {"href": "https://publications.scilifelab.se/researcher/be15218445df4636ab47e1a1dcf8d69c.json"}}, {"family": "Fu", "given": "Jianping", "initials": "J", "orcid": "0000-0001-9629-6739", "researcher": {"href": "https://publications.scilifelab.se/researcher/127bba88c5534146ae9ba1b10929bb4b.json"}}, {"family": "Petropoulos", "given": "Sophie", "initials": "S", "orcid": "0000-0003-2293-8238", "researcher": {"href": "https://publications.scilifelab.se/researcher/0766fd90df1f4199a000eb84b06e2d31.json"}}, {"family": "Lanner", "given": "Fredrik", "initials": "F", "orcid": "0000-0002-2771-7445", "researcher": {"href": "https://publications.scilifelab.se/researcher/ba53ce48a35d413eb86cd104488ce669.json"}}], "type": "journal-article", "published": "2025-01-00", "journal": {"title": "Nat Methods", "issn": "1548-7091", "issn-l": "1548-7091", "volume": "22", "issue": "1", "pages": "193-206"}, "abstract": "Stem cell-based embryo models offer unprecedented experimental tools for studying early human development. The usefulness of embryo models hinges on their molecular, cellular and structural fidelities to their in vivo counterparts. To authenticate human embryo models, single-cell RNA sequencing has been utilized for unbiased transcriptional profiling. However, an organized and integrated human single-cell RNA-sequencing dataset, serving as a universal reference for benchmarking human embryo models, remains unavailable. Here we developed such a reference through the integration of six published human datasets covering development from the zygote to the gastrula. Lineage annotations are contrasted and validated with available human and nonhuman primate datasets. Using stabilized Uniform Manifold Approximation and Projection, we constructed an early embryogenesis prediction tool, where query datasets can be projected on the reference and annotated with predicted cell identities. Using this reference tool, we examined published human embryo models, highlighting the risk of misannotation when relevant references are not utilized for benchmarking and authentication.", "doi": "10.1038/s41592-024-02493-2", "pmid": "39543283", "labels": {"Bioinformatics Support, Infrastructure and Training": "Collaborative", "Bioinformatics Long-term Support WABI": "Collaborative", "Bioinformatics (NBIS)": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC11725501"}, {"db": "pii", "key": "10.1038/s41592-024-02493-2"}], "notes": [], "created": "2024-11-14T12:28:22.114Z", "modified": "2025-12-04T19:32:09.248Z"}, {"entity": "publication", "iuid": "d113d0c37b074a75b7a8dfb5c565524f", "links": {"self": {"href": "https://publications.scilifelab.se/publication/d113d0c37b074a75b7a8dfb5c565524f.json"}, "display": {"href": "https://publications.scilifelab.se/publication/d113d0c37b074a75b7a8dfb5c565524f"}}, "title": "Amnion signals are essential for mesoderm formation in primates.", "authors": [{"family": "Yang", "given": "Ran", "initials": "R"}, {"family": "Goedel", "given": "Alexander", "initials": "A", "orcid": "0000-0002-5980-2257", "researcher": {"href": "https://publications.scilifelab.se/researcher/866e8580c3d8462da0f3ee3b8909ea6f.json"}}, {"family": "Kang", "given": "Yu", "initials": "Y"}, {"family": "Si", "given": "Chenyang", "initials": "C"}, {"family": "Chu", "given": "Chu", "initials": "C"}, {"family": "Zheng", "given": "Yi", "initials": "Y"}, {"family": "Chen", "given": "Zhenzhen", "initials": "Z"}, {"family": "Gruber", "given": "Peter J", "initials": "PJ", "orcid": "0000-0002-7356-905X", "researcher": {"href": "https://publications.scilifelab.se/researcher/623bc96d071d410c8199b178a1e31808.json"}}, {"family": "Xiao", "given": "Yao", "initials": "Y"}, {"family": "Zhou", "given": "Chikai", "initials": "C", "orcid": "0000-0001-9653-3466", "researcher": {"href": "https://publications.scilifelab.se/researcher/05559fcfc7724eb1bf06305a907b58d4.json"}}, {"family": "Witman", "given": "Nevin", "initials": "N"}, {"family": "Eroglu", "given": "Elif", "initials": "E"}, {"family": "Leung", "given": "Chuen-Yan", "initials": "CY"}, {"family": "Chen", "given": "Yongchang", "initials": "Y"}, {"family": "Fu", "given": "Jianping", "initials": "J", "orcid": "0000-0001-9629-6739", "researcher": {"href": "https://publications.scilifelab.se/researcher/127bba88c5534146ae9ba1b10929bb4b.json"}}, {"family": "Ji", "given": "Weizhi", "initials": "W", "orcid": "0000-0003-2550-4224", "researcher": {"href": "https://publications.scilifelab.se/researcher/9e1228641df34f3fa2f8b961d414233b.json"}}, {"family": "Lanner", "given": "Fredrik", "initials": "F", "orcid": "0000-0002-2771-7445", "researcher": {"href": "https://publications.scilifelab.se/researcher/ba53ce48a35d413eb86cd104488ce669.json"}}, {"family": "Niu", "given": "Yuyu", "initials": "Y"}, {"family": "Chien", "given": "Kenneth R", "initials": "KR", "orcid": "0000-0002-2759-8378", "researcher": {"href": "https://publications.scilifelab.se/researcher/971382878b474966bbe58acaa1585999.json"}}], "type": "journal article", "published": "2021-08-26", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "12", "issue": "1", "pages": "5126", "issn-l": "2041-1723"}, "abstract": "Embryonic development is largely conserved among mammals. However, certain genes show divergent functions. By generating a transcriptional atlas containing >30,000 cells from post-implantation non-human primate embryos, we uncover that ISL1, a gene with a well-established role in cardiogenesis, controls a gene regulatory network in primate amnion. CRISPR/Cas9-targeting of ISL1 results in non-human primate embryos which do not yield viable offspring, demonstrating that ISL1 is critically required in primate embryogenesis. On a cellular level, mutant ISL1 embryos display a failure in mesoderm formation due to reduced BMP4 signaling from the amnion. Via loss of function and rescue studies in human embryonic stem cells we confirm a similar role of ISL1 in human in vitro derived amnion. This study highlights the importance of the amnion as a signaling center during primate mesoderm formation and demonstrates the potential of in vitro primate model systems to dissect the genetics of early human embryonic development.", "doi": "10.1038/s41467-021-25186-2", "pmid": "34446705", "labels": {"Bioinformatics Support, Infrastructure and Training": "Service", "Bioinformatics Support and Infrastructure": "Service", "Eukaryotic Single Cell Genomics (ESCG)": "Service", "National Genomics Infrastructure": "Service", "NGI Stockholm (Genomics Production)": "Service", "NGI Stockholm (Genomics Applications)": "Service", "Bioinformatics Support for Computational Resources": "Service", "Bioinformatics (NBIS)": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC8390679"}, {"db": "pii", "key": "10.1038/s41467-021-25186-2"}], "notes": [], "created": "2021-08-30T07:44:40.804Z", "modified": "2024-01-16T13:48:38.655Z"}, {"entity": "publication", "iuid": "a8c3d4fd270e400c9d3da9c6d8a6df9d", "links": {"self": {"href": "https://publications.scilifelab.se/publication/a8c3d4fd270e400c9d3da9c6d8a6df9d.json"}, "display": {"href": "https://publications.scilifelab.se/publication/a8c3d4fd270e400c9d3da9c6d8a6df9d"}}, "title": "Evaluating totipotency using criteria of increasing stringency.", "authors": [{"family": "Posfai", "given": "Eszter", "initials": "E", "orcid": "0000-0002-4871-6652", "researcher": {"href": "https://publications.scilifelab.se/researcher/1e48e0d903c6461c80862a1ba7f93bc9.json"}}, {"family": "Schell", "given": "John Paul", "initials": "JP"}, {"family": "Janiszewski", "given": "Adrian", "initials": "A"}, {"family": "Rovic", "given": "Isidora", "initials": "I"}, {"family": "Murray", "given": "Alexander", "initials": "A"}, {"family": "Bradshaw", "given": "Brian", "initials": "B"}, {"family": "Yamakawa", "given": "Tatsuya", "initials": "T"}, {"family": "Pardon", "given": "Tine", "initials": "T"}, {"family": "El Bakkali", "given": "Mouna", "initials": "M"}, {"family": "Talon", "given": "Irene", "initials": "I"}, {"family": "De Geest", "given": "Natalie", "initials": "N"}, {"family": "Kumar", "given": "Pankaj", "initials": "P", "orcid": "0000-0002-8411-1634", "researcher": {"href": "https://publications.scilifelab.se/researcher/5e9109710e2e45009c332e257c3c498a.json"}}, {"family": "To", "given": "San Kit", "initials": "SK", "orcid": "0000-0002-3057-2902", "researcher": {"href": "https://publications.scilifelab.se/researcher/844ba6692612452cb0d28ab1b3cbcfa4.json"}}, {"family": "Petropoulos", "given": "Sophie", "initials": "S"}, {"family": "Jurisicova", "given": "Andrea", "initials": "A"}, {"family": "Pasque", "given": "Vincent", "initials": "V", "orcid": "0000-0002-5129-0146", "researcher": {"href": "https://publications.scilifelab.se/researcher/78a401cc80624c45bd38f643dd36797b.json"}}, {"family": "Lanner", "given": "Fredrik", "initials": "F", "orcid": "0000-0002-2771-7445", "researcher": {"href": "https://publications.scilifelab.se/researcher/ba53ce48a35d413eb86cd104488ce669.json"}}, {"family": "Rossant", "given": "Janet", "initials": "J", "orcid": "0000-0002-3731-5466", "researcher": {"href": "https://publications.scilifelab.se/researcher/be15218445df4636ab47e1a1dcf8d69c.json"}}], "type": "journal article", "published": "2021-01-00", "journal": {"title": "Nat Cell Biol", "issn": "1476-4679", "volume": "23", "issue": "1", "pages": "49-60", "issn-l": null}, "abstract": "Totipotency is the ability of a single cell to give rise to all of the differentiated cell types that build the conceptus, yet how to capture this property in vitro remains incompletely understood. Defining totipotency relies on a variety of assays of variable stringency. Here, we describe criteria to define totipotency. We explain how distinct criteria of increasing stringency can be used to judge totipotency by evaluating candidate totipotent cell types in mice, including early blastomeres and expanded or extended pluripotent stem cells. Our data challenge the notion that expanded or extended pluripotent states harbour increased totipotent potential relative to conventional embryonic stem cells under in vitro and in vivo conditions.", "doi": "10.1038/s41556-020-00609-2", "pmid": "33420491", "labels": {"NGI Stockholm (Genomics Production)": null, "NGI Stockholm (Genomics Applications)": null, "National Genomics Infrastructure": null, "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pii", "key": "10.1038/s41556-020-00609-2"}], "notes": [], "created": "2021-06-09T12:16:20.850Z", "modified": "2024-01-16T13:48:41.059Z"}, {"entity": "publication", "iuid": "43ac5630ef6c48389d5164ecaf4a37f9", "links": {"self": {"href": "https://publications.scilifelab.se/publication/43ac5630ef6c48389d5164ecaf4a37f9.json"}, "display": {"href": "https://publications.scilifelab.se/publication/43ac5630ef6c48389d5164ecaf4a37f9"}}, "title": "Preclinical safety studies of human embryonic stem cell-derived retinal pigment epithelial cells for the treatment of age-related macular degeneration.", "authors": [{"family": "Petrus-Reurer", "given": "Sandra", "initials": "S", "orcid": "0000-0002-7051-1741", "researcher": {"href": "https://publications.scilifelab.se/researcher/716f74c81a3240e5b612dab55e997267.json"}}, {"family": "Kumar", "given": "Pankaj", "initials": "P", "orcid": "0000-0002-8411-1634", "researcher": {"href": "https://publications.scilifelab.se/researcher/5e9109710e2e45009c332e257c3c498a.json"}}, {"family": "Padrell S\u00e1nchez", "given": "Sara", "initials": "S"}, {"family": "Aronsson", "given": "Monica", "initials": "M"}, {"family": "Andr\u00e9", "given": "Helder", "initials": "H"}, {"family": "Bartuma", "given": "Hammurabi", "initials": "H"}, {"family": "Plaza Reyes", "given": "Alvaro", "initials": "A"}, {"family": "Nandrot", "given": "Emeline F", "initials": "EF"}, {"family": "Kvanta", "given": "Anders", "initials": "A"}, {"family": "Lanner", "given": "Fredrik", "initials": "F", "orcid": "0000-0002-2771-7445", "researcher": {"href": "https://publications.scilifelab.se/researcher/ba53ce48a35d413eb86cd104488ce669.json"}}], "type": "journal article", "published": "2020-08-00", "journal": {"title": "Stem Cells Transl Med", "issn": "2157-6580", "issn-l": "2157-6564", "volume": "9", "issue": "8", "pages": "936-953"}, "abstract": "As pluripotent stem cell (PSC)-based reparative cell therapies are reaching the bedside, there is a growing need for the standardization of studies concerning safety of the derived products. Clinical trials using these promising strategies are in development, and treatment for age-related macular degeneration is one of the first that has reached patients. We have previously established a xeno-free and defined differentiation protocol to generate functional human embryonic stem cells (hESCs)-derived retinal pigment epithelial (RPE) cells. In this study, we perform preclinical safety studies including karyotype and whole-genome sequencing (WGS) to assess genome stability, single-cell RNA sequencing to ensure cell purity, and biodistribution and tumorigenicity analysis to rule out potential migratory or tumorigenic properties of these cells. WGS analysis illustrates that existing germline variants load is higher than the introduced variants acquired through in vitro culture or differentiation, and enforces the importance to examine the genome integrity at a deeper level than just karyotype. Altogether, we provide a strategy for preclinical evaluation of PSC-based therapies and the data support safety of the hESC-RPE cells generated through our in vitro differentiation methodology.", "doi": "10.1002/sctm.19-0396", "pmid": "32319201", "labels": {"Eukaryotic Single Cell Genomics (ESCG)": "Service", "NGI Stockholm (Genomics Production)": "Service", "NGI Stockholm (Genomics Applications)": "Service", "National Genomics Infrastructure": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC7381808"}], "notes": [], "created": "2020-10-06T11:06:47.295Z", "modified": "2024-01-16T13:48:42.005Z"}, {"entity": "publication", "iuid": "35ceb41b656a47a982d5b274b2ccd969", "links": {"self": {"href": "https://publications.scilifelab.se/publication/35ceb41b656a47a982d5b274b2ccd969.json"}, "display": {"href": "https://publications.scilifelab.se/publication/35ceb41b656a47a982d5b274b2ccd969"}}, "title": "Identification of cell surface markers and establishment of monolayer differentiation to retinal pigment epithelial cells.", "authors": [{"family": "Plaza Reyes", "given": "Alvaro", "initials": "A", "orcid": "0000-0003-1167-6316", "researcher": {"href": "https://publications.scilifelab.se/researcher/36f7ddaf16ac45bfad26c96f292f2072.json"}}, {"family": "Petrus-Reurer", "given": "Sandra", "initials": "S", "orcid": "0000-0002-7051-1741", "researcher": {"href": "https://publications.scilifelab.se/researcher/716f74c81a3240e5b612dab55e997267.json"}}, {"family": "Padrell S\u00e1nchez", "given": "Sara", "initials": "S"}, {"family": "Kumar", "given": "Pankaj", "initials": "P", "orcid": "0000-0002-8411-1634", "researcher": {"href": "https://publications.scilifelab.se/researcher/5e9109710e2e45009c332e257c3c498a.json"}}, {"family": "Douagi", "given": "Iyadh", "initials": "I", "orcid": "0000-0002-3221-8667", "researcher": {"href": "https://publications.scilifelab.se/researcher/5a19504ddb2544bca5aeab9abf885b13.json"}}, {"family": "Bartuma", "given": "Hammurabi", "initials": "H"}, {"family": "Aronsson", "given": "Monica", "initials": "M"}, {"family": "Westman", "given": "Sofie", "initials": "S"}, {"family": "Lardner", "given": "Emma", "initials": "E"}, {"family": "Andr\u00e9", "given": "Helder", "initials": "H", "orcid": "0000-0002-2926-2376", "researcher": {"href": "https://publications.scilifelab.se/researcher/5e7098d1d25b4f31a2a45d41ab11da2f.json"}}, {"family": "Falk", "given": "Anna", "initials": "A"}, {"family": "Nandrot", "given": "Emeline F", "initials": "EF", "orcid": "0000-0003-3087-078X", "researcher": {"href": "https://publications.scilifelab.se/researcher/ed12259fa5b24f04853b930077e50077.json"}}, {"family": "Kvanta", "given": "Anders", "initials": "A"}, {"family": "Lanner", "given": "Fredrik", "initials": "F", "orcid": "0000-0002-2771-7445", "researcher": {"href": "https://publications.scilifelab.se/researcher/ba53ce48a35d413eb86cd104488ce669.json"}}], "type": "journal article", "published": "2020-03-30", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "11", "issue": "1", "pages": "1609", "issn-l": "2041-1723"}, "abstract": "In vitro differentiation of human pluripotent stem cells into functional retinal pigment epithelial (RPE) cells provides a potentially unlimited source for cell based reparative therapy of age-related macular degeneration. Although the inherent pigmentation of the RPE cells have been useful to grossly evaluate differentiation efficiency and allowed manual isolation of pigmented structures, accurate quantification and automated isolation has been challenging. To address this issue, here we perform a comprehensive antibody screening and identify cell surface markers for RPE cells. We show that these markers can be used to isolate RPE cells during in vitro differentiation and to track, quantify and improve differentiation efficiency. Finally, these surface markers aided to develop a robust, direct and scalable monolayer differentiation protocol on human recombinant laminin-111 and -521 without the need for manual isolation.", "doi": "10.1038/s41467-020-15326-5", "pmid": "32231223", "labels": {"Eukaryotic Single Cell Genomics (ESCG)": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pii", "key": "10.1038/s41467-020-15326-5"}, {"db": "pmc", "key": "PMC7105463"}], "notes": [], "created": "2020-10-06T11:08:22.218Z", "modified": "2024-01-16T13:48:42.729Z"}, {"entity": "publication", "iuid": "a223b2180f754d79971096d57b714ee6", "links": {"self": {"href": "https://publications.scilifelab.se/publication/a223b2180f754d79971096d57b714ee6.json"}, "display": {"href": "https://publications.scilifelab.se/publication/a223b2180f754d79971096d57b714ee6"}}, "title": "Single-cell analysis of human ovarian cortex identifies distinct cell populations but no oogonial stem cells.", "authors": [{"family": "Wagner", "given": "Magdalena", "initials": "M", "orcid": "0000-0002-6266-1248", "researcher": {"href": "https://publications.scilifelab.se/researcher/124ac27397f845a68732369680da7c5a.json"}}, {"family": "Yoshihara", "given": "Masahito", "initials": "M", "orcid": "0000-0002-8915-9282", "researcher": {"href": "https://publications.scilifelab.se/researcher/4303ab7a18254bb59f8e2386a7f7fb0a.json"}}, {"family": "Douagi", "given": "Iyadh", "initials": "I", "orcid": "0000-0002-3221-8667", "researcher": {"href": "https://publications.scilifelab.se/researcher/5a19504ddb2544bca5aeab9abf885b13.json"}}, {"family": "Damdimopoulos", "given": "Anastasios", "initials": "A"}, {"family": "Panula", "given": "Sarita", "initials": "S"}, {"family": "Petropoulos", "given": "Sophie", "initials": "S"}, {"family": "Lu", "given": "Haojiang", "initials": "H"}, {"family": "Pettersson", "given": "Karin", "initials": "K"}, {"family": "Palm", "given": "Kerstin", "initials": "K"}, {"family": "Katayama", "given": "Shintaro", "initials": "S"}, {"family": "Hovatta", "given": "Outi", "initials": "O", "orcid": "0000-0001-5395-3807", "researcher": {"href": "https://publications.scilifelab.se/researcher/f822ca12dfe0462b99c06e57cace4ce0.json"}}, {"family": "Kere", "given": "Juha", "initials": "J", "orcid": "0000-0003-1974-0271", "researcher": {"href": "https://publications.scilifelab.se/researcher/102085fb1c3147ceaf8dcb7651df1303.json"}}, {"family": "Lanner", "given": "Fredrik", "initials": "F", "orcid": "0000-0002-2771-7445", "researcher": {"href": "https://publications.scilifelab.se/researcher/ba53ce48a35d413eb86cd104488ce669.json"}}, {"family": "Damdimopoulou", "given": "Pauliina", "initials": "P", "orcid": "0000-0001-8458-0855", "researcher": {"href": "https://publications.scilifelab.se/researcher/1d258c0f0d9b417ea4608769fc3ebaaf.json"}}], "type": "journal article", "published": "2020-03-02", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "11", "issue": "1", "pages": "1147", "issn-l": "2041-1723"}, "abstract": "The human ovary orchestrates sex hormone production and undergoes monthly structural changes to release mature oocytes. The outer lining of the ovary (cortex) has a key role in defining fertility in women as it harbors the ovarian reserve. It has been postulated that putative oogonial stem cells exist in the ovarian cortex and that these can be captured by DDX4 antibody isolation. Here, we report single-cell transcriptomes and cell surface antigen profiles of over 24,000 cells from high quality ovarian cortex samples from 21 patients. Our data identify transcriptional profiles of six main cell types; oocytes, granulosa cells, immune cells, endothelial cells, perivascular cells, and stromal cells. Cells captured by DDX4 antibody are perivascular cells, not oogonial stem cells. Our data do not support the existence of germline stem cells in adult human ovaries, thereby reinforcing the dogma of a limited ovarian reserve.", "doi": "10.1038/s41467-020-14936-3", "pmid": "32123174", "labels": {"Eukaryotic Single Cell Genomics (ESCG)": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pii", "key": "10.1038/s41467-020-14936-3"}, {"db": "pmc", "key": "PMC7052271"}], "notes": [], "created": "2020-03-03T10:20:08.878Z", "modified": "2024-01-16T13:48:42.834Z"}]}