{"entity": "researcher", "timestamp": "2026-07-22T16:43:13.579Z", "family": "Das", "given": "Hrishikesh", "initials": "H", "orcid": "0000-0001-7495-7065", "affiliations": ["Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/b150c8ccf1164aaab678a9f6c1acd97b.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/b150c8ccf1164aaab678a9f6c1acd97b"}}, "publications": [{"entity": "publication", "iuid": "d28325d47fdb47cda393a1e60d677055", "links": {"self": {"href": "https://publications.scilifelab.se/publication/d28325d47fdb47cda393a1e60d677055.json"}, "display": {"href": "https://publications.scilifelab.se/publication/d28325d47fdb47cda393a1e60d677055"}}, "title": "Multi-compartmental diversification of neutralizing antibody lineages dissected in SARS-CoV-2 spike-immunized macaques.", "authors": [{"family": "Mandolesi", "given": "Marco", "initials": "M", "orcid": "0000-0003-2927-7831", "researcher": {"href": "https://publications.scilifelab.se/researcher/29e6c31be1704c97a2853aca0833f6b0.json"}}, {"family": "Das", "given": "Hrishikesh", "initials": "H", "orcid": "0000-0001-7495-7065", "researcher": {"href": "https://publications.scilifelab.se/researcher/b150c8ccf1164aaab678a9f6c1acd97b.json"}}, {"family": "de Vries", "given": "Liset", "initials": "L", "orcid": "0000-0002-4797-086X", "researcher": {"href": "https://publications.scilifelab.se/researcher/a6b05b7eec7c4120b4bee2ff15ac8035.json"}}, {"family": "Yang", "given": "Yiqiu", "initials": "Y"}, {"family": "Kim", "given": "Changil", "initials": "C"}, {"family": "Dhinakaran", "given": "Manojj", "initials": "M", "orcid": "0000-0003-2536-8699", "researcher": {"href": "https://publications.scilifelab.se/researcher/de859a6247424dd285f56b841dc23a36.json"}}, {"family": "Castro Dopico", "given": "Xaquin", "initials": "X", "orcid": "0000-0002-9005-6774", "researcher": {"href": "https://publications.scilifelab.se/researcher/cf1d266d61654951a684193993ca53b1.json"}}, {"family": "Fischbach", "given": "Julian", "initials": "J", "orcid": "0000-0001-9411-9181", "researcher": {"href": "https://publications.scilifelab.se/researcher/20e4fea1f9f54727b1bf3ef1cb8f81a4.json"}}, {"family": "Kim", "given": "Sungyong", "initials": "S"}, {"family": "Guryleva", "given": "Mariia V", "initials": "MV"}, {"family": "\u00c0dori", "given": "Monika", "initials": "M"}, {"family": "Chernyshev", "given": "Mark", "initials": "M", "orcid": "0000-0003-1622-9240", "researcher": {"href": "https://publications.scilifelab.se/researcher/678134399edb44afac5e7eacc8622fcb.json"}}, {"family": "St\u00e5lmarck", "given": "Aron", "initials": "A"}, {"family": "Hanke", "given": "Leo", "initials": "L", "orcid": "0000-0001-5514-2418", "researcher": {"href": "https://publications.scilifelab.se/researcher/ece050a286f946f6807170cffc9320e7.json"}}, {"family": "McInerney", "given": "Gerald M", "initials": "GM", "orcid": "0000-0003-2257-7241", "researcher": {"href": "https://publications.scilifelab.se/researcher/5ac2f68095fe4426b97ec070865e5091.json"}}, {"family": "Sheward", "given": "Daniel J", "initials": "DJ", "orcid": "0000-0002-0227-5636", "researcher": {"href": "https://publications.scilifelab.se/researcher/6105f3617cdf4241bf335299ac957ee5.json"}}, {"family": "Corcoran", "given": "Martin", "initials": "M"}, {"family": "H\u00e4llberg", "given": "B Martin", "initials": "BM", "orcid": "0000-0002-6781-0345", "researcher": {"href": "https://publications.scilifelab.se/researcher/2b7e4327d0ac48288afd6061f149156d.json"}}, {"family": "Murrell", "given": "Ben", "initials": "B", "orcid": "0000-0002-0393-4445", "researcher": {"href": "https://publications.scilifelab.se/researcher/8a899203048943489bf7b6310a32b19f.json"}}, {"family": "Karlsson Hedestam", "given": "Gunilla B", "initials": "GB", "orcid": "0000-0001-7255-9047", "researcher": {"href": "https://publications.scilifelab.se/researcher/ad611ac9c76e44989e585edc4d7ff713.json"}}], "type": "journal article", "published": "2024-07-27", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "15", "issue": "1", "pages": "6338", "issn-l": "2041-1723"}, "abstract": "The continued evolution of SARS-CoV-2 underscores the need to understand qualitative aspects of the humoral immune response elicited by spike immunization. Here, we combine monoclonal antibody (mAb) isolation with deep B cell receptor (BCR) repertoire sequencing of rhesus macaques immunized with prefusion-stabilized spike glycoprotein. Longitudinal tracing of spike-sorted B cell lineages in multiple immune compartments demonstrates increasing somatic hypermutation and broad dissemination of vaccine-elicited B cells in draining and non-draining lymphoid compartments, including the bone marrow, spleen and, most notably, periaortic lymph nodes. Phylogenetic analysis of spike-specific monoclonal antibody lineages identified through deep repertoire sequencing delineates extensive intra-clonal diversification that shaped neutralizing activity. Structural analysis of the spike in complex with a broadly neutralizing mAb provides a molecular basis for the observed differences in neutralization breadth between clonally related antibodies. Our findings highlight that immunization leads to extensive intra-clonal B cell evolution where members of the same lineage can both retain the original epitope specificity and evolve to recognize additional spike variants not previously encountered.", "doi": "10.1038/s41467-024-50286-0", "pmid": "39068149", "labels": {"NGI Single cell": "Service", "NGI Stockholm (Genomics Production)": "Service", "National Genomics Infrastructure": "Service", "NGI Stockholm (Genomics Applications)": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11283548"}, {"db": "pii", "key": "10.1038/s41467-024-50286-0"}], "notes": [], "created": "2024-10-14T13:07:16.627Z", "modified": "2024-11-25T10:17:09.411Z"}]}