{"entity": "researcher", "timestamp": "2026-07-15T17:13:32.653Z", "family": "Vallianatou", "given": "Theodosia", "initials": "T", "orcid": "0000-0002-1477-7756", "affiliations": ["Medical Mass Spectrometry Imaging, Department of Pharmaceutical Biosciences, Uppsala University, Uppsala, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/ae610b7669754328a119654fdfcd6af4.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/ae610b7669754328a119654fdfcd6af4"}}, "publications": [{"entity": "publication", "iuid": "6072fdc2c2a64ec5ad2b7aa1a93ddf0b", "links": {"self": {"href": "https://publications.scilifelab.se/publication/6072fdc2c2a64ec5ad2b7aa1a93ddf0b.json"}, "display": {"href": "https://publications.scilifelab.se/publication/6072fdc2c2a64ec5ad2b7aa1a93ddf0b"}}, "title": "Met-ID: An Open-Source Software for Comprehensive Annotation of Multiple On-Tissue Chemical Modifications in MALDI-MSI.", "authors": [{"family": "Bj\u00e4rterot", "given": "Patrik", "initials": "P"}, {"family": "Nilsson", "given": "Anna", "initials": "A"}, {"family": "Shariatgorji", "given": "Reza", "initials": "R"}, {"family": "Vallianatou", "given": "Theodosia", "initials": "T", "orcid": "0000-0002-1477-7756", "researcher": {"href": "https://publications.scilifelab.se/researcher/ae610b7669754328a119654fdfcd6af4.json"}}, {"family": "Kaya", "given": "Ibrahim", "initials": "I"}, {"family": "Svenningsson", "given": "Per", "initials": "P"}, {"family": "K\u00e4ll", "given": "Lukas", "initials": "L", "orcid": "0000-0001-5689-9797", "researcher": {"href": "https://publications.scilifelab.se/researcher/9c9f4444f83e43d79c4772a430ca3969.json"}}, {"family": "Andr\u00e9n", "given": "Per E", "initials": "PE", "orcid": "0000-0002-4062-7743", "researcher": {"href": "https://publications.scilifelab.se/researcher/64f6381de42949db8d30b56b526f3e26.json"}}], "type": "journal article", "published": "2025-04-29", "journal": {"title": "Anal. Chem.", "issn": "1520-6882", "volume": "97", "issue": "16", "pages": "9033-9041", "issn-l": "0003-2700"}, "abstract": "Here, we introduce Met-ID, a graphical user interface software designed to efficiently identify metabolites from MALDI-MSI data sets. Met-ID enables annotation of m/z features from any type of MALDI-MSI experiment, involving either derivatizing or conventional matrices. It utilizes structural information for derivatizing matrices to generate a subset of targets that contain only functional groups specific to the derivatization agent. The software is able to identify multiple derivatization sites on the same molecule, facilitating identification of the derivatized compound. This ability is exemplified by FMP-10, a reactive matrix that assists the covalent charge-tagging of molecules containing phenolic hydroxyl and/or primary or secondary amine groups. Met-ID also permits users to recalibrate data with known m/z ratios, boosting confidence in mass match results. Furthermore, Met-ID includes a database featuring MS2 spectra of numerous chemical standards, consisting of neurotransmitters and metabolites derivatized with FMP-10, alongside peaks for FMP-10 itself, all accessible directly through the software. The MS2 spectral database supports user-uploaded spectra and enables comparison of these spectra with user-provided tissue MS2 spectra for similarity assessment. Although initially installed with basic data, Met-ID is designed to be customizable, encouraging users to tailor the software to their specific needs. While several MSI-oriented software solutions exist, Met-ID combines both MS1 and MS2 functionalities. Developed in alignment with the FAIR Guiding Principles for scientific software, Met-ID is freely available as an open-source tool on GitHub, ensuring wide accessibility and collaboration.", "doi": "10.1021/acs.analchem.5c00633", "pmid": "40253716", "labels": {"Spatial Mass Spectrometry": "Technology development"}, "xrefs": [{"db": "pmc", "key": "PMC12044586"}], "notes": [], "created": "2025-11-21T09:59:55.456Z", "modified": "2025-11-21T09:59:55.515Z"}, {"entity": "publication", "iuid": "ad5916e84efd42529a1c95bb30f10292", "links": {"self": {"href": "https://publications.scilifelab.se/publication/ad5916e84efd42529a1c95bb30f10292.json"}, "display": {"href": "https://publications.scilifelab.se/publication/ad5916e84efd42529a1c95bb30f10292"}}, "title": "Applying Spatial Metabolomics To Investigate Age- and Drug-Induced Neurochemical Changes.", "authors": [{"family": "Vallianatou", "given": "Theodosia", "initials": "T", "orcid": "0000-0002-1477-7756", "researcher": {"href": "https://publications.scilifelab.se/researcher/ae610b7669754328a119654fdfcd6af4.json"}}, {"family": "Angerer", "given": "Tina B", "initials": "TB", "orcid": "0000-0003-3852-6254", "researcher": {"href": "https://publications.scilifelab.se/researcher/c3f5ed541c9f4e2bb6c7c7519d6ad36b.json"}}, {"family": "Kaya", "given": "Ibrahim", "initials": "I"}, {"family": "Nilsson", "given": "Anna", "initials": "A"}, {"family": "Shariatgorji", "given": "Reza", "initials": "R"}, {"family": "Svenningsson", "given": "Per", "initials": "P"}, {"family": "Andr\u00e9n", "given": "Per E", "initials": "PE", "orcid": "0000-0002-4062-7743", "researcher": {"href": "https://publications.scilifelab.se/researcher/64f6381de42949db8d30b56b526f3e26.json"}}], "type": "journal article", "published": "2024-08-07", "journal": {"title": "ACS Chem Neurosci", "issn": "1948-7193", "volume": "15", "issue": "15", "pages": "2822-2829", "issn-l": "1948-7193"}, "abstract": "In an era when population aging is increasing the burden of neurodegenerative conditions, deciphering the mechanisms underlying brain senescence is more important than ever. Here, we present a spatial metabolomics analysis of age-induced neurochemical alterations in the mouse brain using negative ionization mode mass spectrometry imaging. The age-dependent effects of the acetylcholinesterase inhibitor tacrine were simultaneously examined. For ultrahigh mass resolution analysis, we utilized a Fourier-transform ion cyclotron resonance spectrometer. To complement this, a trapped ion mobility spectrometry time-of-flight analyzer provided high speed and lateral resolution. The chosen approach facilitated the detection and identification of a wide range of metabolites, from amino acids to sphingolipids. We reported significant, age-dependent alterations in brain lipids which were most evident for sulfatides and lysophosphatidic acids. Sulfatide species, which are mainly localized to white matter, either increased or decreased with age, depending on the carbon chain length and hydroxylation stage. Lysophosphatidic acids were found to decrease with age in the detailed cortical and hippocampal subregions. An age-dependent increase in the glutamine/glutamate ratio, an indicator of glia-neuron interconnection and neurotoxicity, was detected after tacrine administration. The presented metabolic mapping approach was able to provide visualizations of the lipid signaling and neurotransmission alterations induced by early aging and can thus be beneficial to further elucidating age-related neurochemical pathways.", "doi": "10.1021/acschemneuro.4c00199", "pmid": "39072364", "labels": {"Spatial Mass Spectrometry": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC11311129"}], "notes": [], "created": "2024-11-20T10:41:26.891Z", "modified": "2024-11-20T10:41:27.370Z"}, {"entity": "publication", "iuid": "a9801178dbec4953a293f9964bcbc340", "links": {"self": {"href": "https://publications.scilifelab.se/publication/a9801178dbec4953a293f9964bcbc340.json"}, "display": {"href": "https://publications.scilifelab.se/publication/a9801178dbec4953a293f9964bcbc340"}}, "title": "Spatial lipidomics reveals brain region-specific changes of sulfatides in an experimental MPTP Parkinson's disease primate model.", "authors": [{"family": "Kaya", "given": "Ibrahim", "initials": "I"}, {"family": "Nilsson", "given": "Anna", "initials": "A"}, {"family": "Lupt\u00e1kov\u00e1", "given": "Dominika", "initials": "D", "orcid": "0000-0002-3691-2584", "researcher": {"href": "https://publications.scilifelab.se/researcher/d87af7268aa8418db2b32c560c291d57.json"}}, {"family": "He", "given": "Yachao", "initials": "Y", "orcid": "0000-0002-0241-2901", "researcher": {"href": "https://publications.scilifelab.se/researcher/80733d0ca6fa45d7b9133ac217de6e18.json"}}, {"family": "Vallianatou", "given": "Theodosia", "initials": "T", "orcid": "0000-0002-1477-7756", "researcher": {"href": "https://publications.scilifelab.se/researcher/ae610b7669754328a119654fdfcd6af4.json"}}, {"family": "Bj\u00e4rterot", "given": "Patrik", "initials": "P", "orcid": "0000-0002-4350-5530", "researcher": {"href": "https://publications.scilifelab.se/researcher/bea1b606a9e149c8846bf0e155c80c6c.json"}}, {"family": "Svenningsson", "given": "Per", "initials": "P", "orcid": "0000-0001-6727-3802", "researcher": {"href": "https://publications.scilifelab.se/researcher/5199496295334771ba3c5621a83a6f43.json"}}, {"family": "Bezard", "given": "Erwan", "initials": "E", "orcid": "0000-0002-0410-4638", "researcher": {"href": "https://publications.scilifelab.se/researcher/c33e3029113549ea96e60c7952e5a19a.json"}}, {"family": "Andr\u00e9n", "given": "Per E", "initials": "PE", "orcid": "0000-0002-4062-7743", "researcher": {"href": "https://publications.scilifelab.se/researcher/64f6381de42949db8d30b56b526f3e26.json"}}], "type": "journal article", "published": "2023-07-26", "journal": {"title": "NPJ Parkinsons Dis", "issn": "2373-8057", "volume": "9", "issue": "1", "pages": "118", "issn-l": null}, "abstract": "Metabolism of MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) to the neurotoxin MPP+ in the brain causes permanent Parkinson's disease-like symptoms by destroying dopaminergic neurons in the pars compacta of the substantia nigra in humans and non-human primates. However, the complete molecular pathology underlying MPTP-induced parkinsonism remains poorly understood. We used dual polarity matrix-assisted laser desorption/ionization mass spectrometry imaging to thoroughly image numerous glycerophospholipids and sphingolipids in coronal brain tissue sections of MPTP-lesioned and control non-human primate brains (Macaca mulatta). The results revealed specific distributions of several sulfatide lipid molecules based on chain-length, number of double bonds, and importantly, hydroxylation stage. More specifically, certain long-chain hydroxylated sulfatides with polyunsaturated chains in the molecular structure were depleted within motor-related brain regions in the MPTP-lesioned animals, e.g., external and internal segments of globus pallidus and substantia nigra pars reticulata. In contrast, certain long-chain non-hydroxylated sulfatides were found to be elevated within the same brain regions. These findings demonstrate region-specific dysregulation of sulfatide metabolism within the MPTP-lesioned macaque brain. The depletion of long-chain hydroxylated sulfatides in the MPTP-induced pathology indicates oxidative stress and oligodendrocyte/myelin damage within the pathologically relevant brain regions. Hence, the presented findings improve our current understanding of the molecular pathology of MPTP-induced parkinsonism within primate brains, and provide a basis for further research regarding the role of dysregulated sulfatide metabolism in PD.", "doi": "10.1038/s41531-023-00558-1", "pmid": "37495571", "labels": {"Spatial Mass Spectrometry": "Technology development"}, "xrefs": [{"db": "pmc", "key": "PMC10372136"}, {"db": "pii", "key": "10.1038/s41531-023-00558-1"}], "notes": [], "created": "2023-11-30T10:15:39.072Z", "modified": "2023-11-30T10:15:39.211Z"}, {"entity": "publication", "iuid": "427143398ef547e2b13bbc005c229189", "links": {"self": {"href": "https://publications.scilifelab.se/publication/427143398ef547e2b13bbc005c229189.json"}, "display": {"href": "https://publications.scilifelab.se/publication/427143398ef547e2b13bbc005c229189"}}, "title": "Region-Specific and Age-Dependent Multitarget Effects of Acetylcholinesterase Inhibitor Tacrine on Comprehensive Neurotransmitter Systems.", "authors": [{"family": "Fridjonsdottir", "given": "Elva", "initials": "E"}, {"family": "Vallianatou", "given": "Theodosia", "initials": "T", "orcid": "0000-0002-1477-7756", "researcher": {"href": "https://publications.scilifelab.se/researcher/ae610b7669754328a119654fdfcd6af4.json"}}, {"family": "Mantas", "given": "Ioannis", "initials": "I"}, {"family": "Shariatgorji", "given": "Reza", "initials": "R", "orcid": "0000-0001-9484-0921", "researcher": {"href": "https://publications.scilifelab.se/researcher/7762e9f6779c4780a4077c557eb7a3b6.json"}}, {"family": "Nilsson", "given": "Anna", "initials": "A"}, {"family": "Schembri", "given": "Luke S", "initials": "LS", "orcid": "0000-0001-5588-1077", "researcher": {"href": "https://publications.scilifelab.se/researcher/9dc760b581324125816ed40eb748104c.json"}}, {"family": "Odell", "given": "Luke R", "initials": "LR", "orcid": "0000-0001-7658-5103", "researcher": {"href": "https://publications.scilifelab.se/researcher/7653ba79ce9d4e4a80be4b42f516680b.json"}}, {"family": "Svenningsson", "given": "Per", "initials": "P"}, {"family": "Andr\u00e9n", "given": "Per E", "initials": "PE", "orcid": "0000-0002-4062-7743", "researcher": {"href": "https://publications.scilifelab.se/researcher/64f6381de42949db8d30b56b526f3e26.json"}}], "type": "journal article", "published": "2022-01-21", "journal": {"title": "ACS Chem. Biol.", "issn": "1554-8937", "volume": "17", "issue": "1", "pages": "147-158", "issn-l": "1554-8929"}, "abstract": "Regional brain distribution and metabolism of neurotransmitters and their response to drug treatment are fundamentally important for understanding the central effects of neuroactive substances. We used matrix-assisted laser desorption/ionization mass spectrometry imaging in combination with multivariate analysis to visualize in anatomical detail metabolic effects of aging and tacrine-mediated acetylcholinesterase inhibition on comprehensive neurotransmitter systems in multiple mouse brain regions of 12-week-old and 14-month-old mice. We detected age-related increases in 3,4-dihydroxyphenylacetaldehyde and histamine, indicating oxidative stress and aging deficits in astrocytes. Tacrine had a significant impact on the metabolism of neurotransmitters in both age groups; predominantly, there was an increased norepinephrine turnover throughout the brain and decreased 3-methoxy tyramine, a marker for dopamine release, in the striatum. The striatal levels of histamine were only elevated after tacrine administration in the older animals. Our results demonstrated that tacrine is a multitarget and region-specific neuroactive agent, inducing age-specific responses. Although well-studied, the complete mechanisms of the action of tacrine are not fully understood, and the current findings reveal features that may help explain its treatment-related effectiveness and central side effects.", "doi": "10.1021/acschembio.1c00803", "pmid": "34932310", "labels": {"Spatial Mass Spectrometry": "Technology development"}, "xrefs": [{"db": "pmc", "key": "PMC8787750"}], "notes": [], "created": "2022-11-16T14:01:55.350Z", "modified": "2022-11-16T14:01:55.392Z"}, {"entity": "publication", "iuid": "374a9ca3be9f4190b84efde8e03ff7fb", "links": {"self": {"href": "https://publications.scilifelab.se/publication/374a9ca3be9f4190b84efde8e03ff7fb.json"}, "display": {"href": "https://publications.scilifelab.se/publication/374a9ca3be9f4190b84efde8e03ff7fb"}}, "title": "Neuropharmacokinetic visualization of regional and subregional unbound antipsychotic drug transport across the blood-brain barrier.", "authors": [{"family": "Lupt\u00e1kov\u00e1", "given": "Dominika", "initials": "D"}, {"family": "Vallianatou", "given": "Theodosia", "initials": "T", "orcid": "0000-0002-1477-7756", "researcher": {"href": "https://publications.scilifelab.se/researcher/ae610b7669754328a119654fdfcd6af4.json"}}, {"family": "Nilsson", "given": "Anna", "initials": "A"}, {"family": "Shariatgorji", "given": "Reza", "initials": "R", "orcid": "0000-0001-9484-0921", "researcher": {"href": "https://publications.scilifelab.se/researcher/7762e9f6779c4780a4077c557eb7a3b6.json"}}, {"family": "Hammarlund-Udenaes", "given": "Margareta", "initials": "M"}, {"family": "Loryan", "given": "Irena", "initials": "I", "orcid": "0000-0002-1557-4416", "researcher": {"href": "https://publications.scilifelab.se/researcher/63688cf99e7448b792734ebf77afef32.json"}}, {"family": "Andr\u00e9n", "given": "Per E", "initials": "PE", "orcid": "0000-0002-4062-7743", "researcher": {"href": "https://publications.scilifelab.se/researcher/64f6381de42949db8d30b56b526f3e26.json"}}], "type": "journal article", "published": "2021-09-03", "journal": {"title": "Mol. Psychiatry", "issn": "1476-5578", "issn-l": "1359-4184"}, "abstract": "Comprehensive determination of the extent of drug transport across the region-specific blood-brain barrier (BBB) is a major challenge in preclinical studies. Multiple approaches are needed to determine the regional free (unbound) drug concentration at which a drug engages with its therapeutic target. We present an approach that merges in vivo and in vitro neuropharmacokinetic investigations with mass spectrometry imaging to quantify and visualize both the extent of unbound drug BBB transport and the post-BBB cerebral distribution of drugs at regional and subregional levels. Direct imaging of the antipsychotic drugs risperidone, clozapine, and olanzapine using this approach enabled differentiation of regional and subregional BBB transport characteristics at 20-\u00b5m resolution in small brain regions, which could not be achieved by other means. Our approach allows investigation of heterogeneity in BBB transport and presents new possibilities for molecular psychiatrists by facilitating interpretation of regional target-site exposure results and decision-making.", "doi": "10.1038/s41380-021-01267-y", "pmid": "34480089", "labels": {"Spatial Mass Spectrometry": "Technology development"}, "xrefs": [{"db": "pii", "key": "10.1038/s41380-021-01267-y"}], "notes": [], "created": "2021-12-03T11:46:46.876Z", "modified": "2021-12-03T11:46:46.965Z"}, {"entity": "publication", "iuid": "1d98cc80efea4170b653e983cc7d9744", "links": {"self": {"href": "https://publications.scilifelab.se/publication/1d98cc80efea4170b653e983cc7d9744.json"}, "display": {"href": "https://publications.scilifelab.se/publication/1d98cc80efea4170b653e983cc7d9744"}}, "title": "Integration of Mass Spectrometry Imaging and Machine Learning Visualizes Region-Specific Age-Induced and Drug-Target Metabolic Perturbations in the Brain.", "authors": [{"family": "Vallianatou", "given": "Theodosia", "initials": "T", "orcid": "0000-0002-1477-7756", "researcher": {"href": "https://publications.scilifelab.se/researcher/ae610b7669754328a119654fdfcd6af4.json"}}, {"family": "Shariatgorji", "given": "Reza", "initials": "R", "orcid": "0000-0001-9484-0921", "researcher": {"href": "https://publications.scilifelab.se/researcher/7762e9f6779c4780a4077c557eb7a3b6.json"}}, {"family": "Nilsson", "given": "Anna", "initials": "A"}, {"family": "Karlgren", "given": "Maria", "initials": "M"}, {"family": "Hulme", "given": "Heather", "initials": "H"}, {"family": "Fridjonsdottir", "given": "Elva", "initials": "E"}, {"family": "Svenningsson", "given": "Per", "initials": "P"}, {"family": "Andr\u00e9n", "given": "Per E", "initials": "PE", "orcid": "0000-0002-4062-7743", "researcher": {"href": "https://publications.scilifelab.se/researcher/64f6381de42949db8d30b56b526f3e26.json"}}], "type": "journal article", "published": "2021-05-19", "journal": {"title": "ACS Chem Neurosci", "issn": "1948-7193", "volume": "12", "issue": "10", "pages": "1811-1823", "issn-l": "1948-7193"}, "abstract": "Detailed metabolic imaging of specific brain regions in early aging may expose pathophysiological mechanisms and indicate effective neuropharmacological targets in the onset of cognitive decline. Comprehensive imaging of brain aging and drug-target effects is restricted using conventional methodology. We simultaneously visualized multiple metabolic alterations induced by normal aging in specific regions of mouse brains by integrating Fourier-transform ion cyclotron resonance mass spectrometry imaging and combined supervised and unsupervised machine learning models. We examined the interplay between aging and the response to tacrine-induced acetylcholinesterase inhibition, a well-characterized therapeutic treatment against dementia. The dipeptide carnosine (\u03b2-alanyl-l-histidine) and the vitamin \u03b1-tocopherol were significantly elevated by aging in different brain regions. l-Carnitine and acetylcholine metabolism were found to be major pathways affected by aging and tacrine administration in a brain region-specific manner, indicating altered mitochondrial function and neurotransmission. The highly interconnected hippocampus and retrosplenial cortex displayed different age-induced alterations in lipids and acylcarnitines, reflecting diverse region-specific metabolic effects. The subregional differences observed in the hippocampal formation of several lipid metabolites demonstrate the unique potential of the technique compared to standard mass spectrometry approaches. An age-induced increase of endogenous antioxidants, such as \u03b1-tocopherol, in the hippocampus was detected, suggesting an augmentation of neuroprotective mechanisms in early aging. Our comprehensive imaging approach visualized heterogeneous age-induced metabolic perturbations in mitochondrial function, neurotransmission, and lipid signaling, not always attenuated by acetylcholinesterase inhibition.", "doi": "10.1021/acschemneuro.1c00103", "pmid": "33939923", "labels": {"Spatial Mass Spectrometry": "Technology development"}, "xrefs": [{"db": "pmc", "key": "PMC8291481"}], "notes": [], "created": "2021-12-03T11:49:03.306Z", "modified": "2021-12-03T11:49:03.347Z"}, {"entity": "publication", "iuid": "aab5be8b28f949029e6e9dafb461eca8", "links": {"self": {"href": "https://publications.scilifelab.se/publication/aab5be8b28f949029e6e9dafb461eca8.json"}, "display": {"href": "https://publications.scilifelab.se/publication/aab5be8b28f949029e6e9dafb461eca8"}}, "title": "Mass spectrometry imaging identifies abnormally elevated brain l-DOPA levels and extrastriatal monoaminergic dysregulation in l-DOPA-induced dyskinesia.", "authors": [{"family": "Fridjonsdottir", "given": "Elva", "initials": "E", "orcid": "0000-0002-3779-3556", "researcher": {"href": "https://publications.scilifelab.se/researcher/d39a6e68683d4414bb87a0dc7033a7a4.json"}}, {"family": "Shariatgorji", "given": "Reza", "initials": "R", "orcid": "0000-0001-9484-0921", "researcher": {"href": "https://publications.scilifelab.se/researcher/7762e9f6779c4780a4077c557eb7a3b6.json"}}, {"family": "Nilsson", "given": "Anna", "initials": "A", "orcid": "0000-0002-6183-6931", "researcher": {"href": "https://publications.scilifelab.se/researcher/79c9fe163d31414e8b66aa0834538627.json"}}, {"family": "Vallianatou", "given": "Theodosia", "initials": "T", "orcid": "0000-0002-1477-7756", "researcher": {"href": "https://publications.scilifelab.se/researcher/ae610b7669754328a119654fdfcd6af4.json"}}, {"family": "Odell", "given": "Luke R", "initials": "LR", "orcid": "0000-0001-7658-5103", "researcher": {"href": "https://publications.scilifelab.se/researcher/7653ba79ce9d4e4a80be4b42f516680b.json"}}, {"family": "Schembri", "given": "Luke S", "initials": "LS", "orcid": "0000-0001-5588-1077", "researcher": {"href": "https://publications.scilifelab.se/researcher/9dc760b581324125816ed40eb748104c.json"}}, {"family": "Svenningsson", "given": "Per", "initials": "P", "orcid": "0000-0001-6727-3802", "researcher": {"href": "https://publications.scilifelab.se/researcher/5199496295334771ba3c5621a83a6f43.json"}}, {"family": "Fernagut", "given": "Pierre-Olivier", "initials": "P"}, {"family": "Crossman", "given": "Alan R", "initials": "AR"}, {"family": "Bezard", "given": "Erwan", "initials": "E", "orcid": "0000-0002-0410-4638", "researcher": {"href": "https://publications.scilifelab.se/researcher/c33e3029113549ea96e60c7952e5a19a.json"}}, {"family": "Andr\u00e9n", "given": "Per E", "initials": "PE", "orcid": "0000-0002-4062-7743", "researcher": {"href": "https://publications.scilifelab.se/researcher/64f6381de42949db8d30b56b526f3e26.json"}}], "type": "journal article", "published": "2021-01-00", "journal": {"title": "Sci Adv", "issn": "2375-2548", "issn-l": "2375-2548", "volume": "7", "issue": "2", "pages": null}, "abstract": "l-DOPA treatment for Parkinson's disease frequently leads to dyskinesias, the pathophysiology of which is poorly understood. We used MALDI-MSI to map the distribution of l-DOPA and monoaminergic pathways in brains of dyskinetic and nondyskinetic primates. We report elevated levels of l-DOPA, and its metabolite 3-O-methyldopa, in all measured brain regions of dyskinetic animals and increases in dopamine and metabolites in all regions analyzed except the striatum. In dyskinesia, dopamine levels correlated well with l-DOPA levels in extrastriatal regions, such as hippocampus, amygdala, bed nucleus of the stria terminalis, and cortical areas, but not in the striatum. Our results demonstrate that l-DOPA-induced dyskinesia is linked to a dysregulation of l-DOPA metabolism throughout the brain. The inability of extrastriatal brain areas to regulate the formation of dopamine during l-DOPA treatment introduces the potential of dopamine or even l-DOPA itself to modulate neuronal signaling widely across the brain, resulting in unwanted side effects.", "doi": "10.1126/sciadv.abe5948", "pmid": "33523980", "labels": {"Spatial Mass Spectrometry": "Technology development"}, "xrefs": [{"db": "pii", "key": "7/2/eabe5948"}, {"db": "pmc", "key": "PMC7787486"}], "notes": [], "created": "2021-03-26T12:47:07.296Z", "modified": "2024-02-09T14:58:49.154Z"}]}