{"entity": "researcher", "timestamp": "2026-07-12T08:54:44.696Z", "family": "Tunstrom", "given": "Kalle", "initials": "K", "orcid": "0000-0002-5285-1531", "affiliations": ["Department of Zoology, Stockholm University, Stockholm, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/abd0ddb97d724542b6e7c46f782f3bbd.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/abd0ddb97d724542b6e7c46f782f3bbd"}}, "publications": [{"entity": "publication", "iuid": "df60b381dc4943cdbbccf12206421777", "links": {"self": {"href": "https://publications.scilifelab.se/publication/df60b381dc4943cdbbccf12206421777.json"}, "display": {"href": "https://publications.scilifelab.se/publication/df60b381dc4943cdbbccf12206421777"}}, "title": "Lineage-specific targets of positive selection in three leaf beetles correspond with defence capacity against their shared parasitoid wasp.", "authors": [{"family": "Yang", "given": "Xuyue", "initials": "X"}, {"family": "Tunstr\u00f6m", "given": "Kalle", "initials": "K", "orcid": "0000-0002-5285-1531", "researcher": {"href": "https://publications.scilifelab.se/researcher/abd0ddb97d724542b6e7c46f782f3bbd.json"}}, {"family": "Slotte", "given": "Tanja", "initials": "T"}, {"family": "Wheat", "given": "Christopher W", "initials": "CW", "orcid": "0000-0003-1863-2340", "researcher": {"href": "https://publications.scilifelab.se/researcher/7e498f04977a48c89ffcd0bae890d4cb.json"}}, {"family": "Hamb\u00e4ck", "given": "Peter A", "initials": "PA", "orcid": "0000-0001-6362-6199", "researcher": {"href": "https://publications.scilifelab.se/researcher/1ddfc67c7c774583861a5ea3774eaa1a.json"}}], "type": "journal article", "published": "2025-09-00", "journal": {"title": "Heredity (Edinb)", "issn": "1365-2540", "volume": "134", "issue": "9", "pages": "567-575", "issn-l": "0018-067X"}, "abstract": "Parasitoid wasps are major causes of mortality of many species, making host immune defences a common target of adaptive evolution, though such targets outside model species are poorly understood. In this study, we used two tests of positive selection to compare across three closely related Galerucella leaf beetles that show substantial differences in their phenotypic response to the shared parasitoid wasp Asecodes parviclava, their main natural enemy. Using a codon-based test, which detects excess amino acid fixations per locus along each species' lineage, we found more evidence of positive selection on parasitoid-relevant immune genes in the species with the strongest immunocompetence (G. pusilla) compared with the species having weaker immunocompetence (G. tenella and G. calmariensis). Moreover, genes coding for the early phases in the immune response cascade were predominantly among the positively selected immune genes, providing targets for future functional genomic study to pin-point connections between genotypic and phenotypic differences in defences towards a parasitoid wasp. In contrast, genome-wide analyses of the haplotype frequency spectrum, which quantify selection over recent evolutionary time scales, revealed similar signatures of positive selection on immune genes across species. These results advance the field of host-parasitoid dynamics by providing novel insights into the tempo and mode of insect host evolutionary dynamics, and offering a framework for making genotype to phenotype connections for immunocompetence phenotypes.", "doi": "10.1038/s41437-025-00794-6", "pmid": "40921792", "labels": {"NGI Short read": "Service", "National Genomics Infrastructure": "Service", "NGI Stockholm (Genomics Production)": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC12457636"}, {"db": "pii", "key": "10.1038/s41437-025-00794-6"}], "notes": [], "created": "2025-09-29T07:36:03.222Z", "modified": "2025-11-21T09:22:48.226Z"}, {"entity": "publication", "iuid": "34ee2d9d31294024a4bd99218824e5c7", "links": {"self": {"href": "https://publications.scilifelab.se/publication/34ee2d9d31294024a4bd99218824e5c7.json"}, "display": {"href": "https://publications.scilifelab.se/publication/34ee2d9d31294024a4bd99218824e5c7"}}, "title": "The genomics and evolution of inter-sexual mimicry and female-limited polymorphisms in damselflies.", "authors": [{"family": "Willink", "given": "Beatriz", "initials": "B", "orcid": "0000-0002-4579-6909", "researcher": {"href": "https://publications.scilifelab.se/researcher/b2484b770760479aa8102b95268b2de5.json"}}, {"family": "Tunstr\u00f6m", "given": "Kalle", "initials": "K", "orcid": "0000-0002-5285-1531", "researcher": {"href": "https://publications.scilifelab.se/researcher/abd0ddb97d724542b6e7c46f782f3bbd.json"}}, {"family": "Nil\u00e9n", "given": "Sofie", "initials": "S", "orcid": "0009-0002-0996-0182", "researcher": {"href": "https://publications.scilifelab.se/researcher/19b63b7c5ebd4b90b6431b33882c2f14.json"}}, {"family": "Chikhi", "given": "Rayan", "initials": "R"}, {"family": "Lemane", "given": "T\u00e9o", "initials": "T"}, {"family": "Takahashi", "given": "Michihiko", "initials": "M", "orcid": "0009-0000-9572-4463", "researcher": {"href": "https://publications.scilifelab.se/researcher/ff33a3b13f4e4a3d9a312d323f3ab2f4.json"}}, {"family": "Takahashi", "given": "Yuma", "initials": "Y", "orcid": "0000-0003-1490-7837", "researcher": {"href": "https://publications.scilifelab.se/researcher/6aaa231bbcb9421a9bca56d8c1a9b86f.json"}}, {"family": "Svensson", "given": "Erik I", "initials": "EI", "orcid": "0000-0001-9006-016X", "researcher": {"href": "https://publications.scilifelab.se/researcher/975e1e8172f64aa78265e6c7772990af.json"}}, {"family": "Wheat", "given": "Christopher West", "initials": "CW", "orcid": "0000-0003-1863-2340", "researcher": {"href": "https://publications.scilifelab.se/researcher/7e498f04977a48c89ffcd0bae890d4cb.json"}}], "type": "journal article", "published": "2023-11-06", "journal": {"title": "Nat Ecol Evol", "issn": "2397-334X", "issn-l": "2397-334X", "volume": null, "issue": null, "pages": null}, "abstract": "Sex-limited morphs can provide profound insights into the evolution and genomic architecture of complex phenotypes. Inter-sexual mimicry is one particular type of sex-limited polymorphism in which a novel morph resembles the opposite sex. While inter-sexual mimics are known in both sexes and a diverse range of animals, their evolutionary origin is poorly understood. Here, we investigated the genomic basis of female-limited morphs and male mimicry in the common bluetail damselfly. Differential gene expression between morphs has been documented in damselflies, but no causal locus has been previously identified. We found that male mimicry originated in an ancestrally sexually dimorphic lineage in association with multiple structural changes, probably driven by transposable element activity. These changes resulted in ~900 kb of novel genomic content that is partly shared by male mimics in a close relative, indicating that male mimicry is a trans-species polymorphism. More recently, a third morph originated following the translocation of part of the male-mimicry sequence into a genomic position ~3.5 mb apart. We provide evidence of balancing selection maintaining male mimicry, in line with previous field population studies. Our results underscore how structural variants affecting a handful of potentially regulatory genes and morph-specific genes can give rise to novel and complex phenotypic polymorphisms.", "doi": "10.1038/s41559-023-02243-1", "pmid": "37932383", "labels": {"NGI Uppsala (Uppsala Genome Center)": "Service", "NGI Long read": "Service", "National Genomics Infrastructure": "Service", "NGI Short read": "Service", "NGI Stockholm (Genomics Production)": null, "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pii", "key": "10.1038/s41559-023-02243-1"}], "notes": [], "created": "2023-11-19T20:04:57.151Z", "modified": "2024-01-16T13:48:31.695Z"}, {"entity": "publication", "iuid": "e7f6c7b32a7744319eae28a8250ba884", "links": {"self": {"href": "https://publications.scilifelab.se/publication/e7f6c7b32a7744319eae28a8250ba884.json"}, "display": {"href": "https://publications.scilifelab.se/publication/e7f6c7b32a7744319eae28a8250ba884"}}, "title": "Evidence for a single, ancient origin of a genus-wide alternative life history strategy.", "authors": [{"family": "Tunstr\u00f6m", "given": "Kalle", "initials": "K", "orcid": "0000-0002-5285-1531", "researcher": {"href": "https://publications.scilifelab.se/researcher/abd0ddb97d724542b6e7c46f782f3bbd.json"}}, {"family": "Woronik", "given": "Alyssa", "initials": "A", "orcid": "0000-0003-3017-6069", "researcher": {"href": "https://publications.scilifelab.se/researcher/841b99e196ed4fe7ad25f76200c98ec6.json"}}, {"family": "Hanly", "given": "Joseph J", "initials": "JJ", "orcid": "0000-0002-9459-9776", "researcher": {"href": "https://publications.scilifelab.se/researcher/92b6972ffe974d0d95f7350a62ec209e.json"}}, {"family": "Rastas", "given": "Pasi", "initials": "P", "orcid": "0000-0003-2768-1339", "researcher": {"href": "https://publications.scilifelab.se/researcher/5a8f5f2160154bdfa0b21dfc132d4871.json"}}, {"family": "Chichvarkhin", "given": "Anton", "initials": "A"}, {"family": "Warren", "given": "Andrew D", "initials": "AD"}, {"family": "Kawahara", "given": "Akito Y", "initials": "AY", "orcid": "0000-0002-3724-4610", "researcher": {"href": "https://publications.scilifelab.se/researcher/fe112868785c4b8187cdc164e25ef9ae.json"}}, {"family": "Schoville", "given": "Sean D", "initials": "SD", "orcid": "0000-0001-7364-434X", "researcher": {"href": "https://publications.scilifelab.se/researcher/c3a4dc2fb0c8434994e065eb9ffad9f5.json"}}, {"family": "Ficarrotta", "given": "Vincent", "initials": "V", "orcid": "0000-0002-4021-4906", "researcher": {"href": "https://publications.scilifelab.se/researcher/3535033454414fc8be454e9880f7dcee.json"}}, {"family": "Porter", "given": "Adam H", "initials": "AH", "orcid": "0000-0002-8154-4207", "researcher": {"href": "https://publications.scilifelab.se/researcher/f49a80fbd4d441e1bf2281a57751b79b.json"}}, {"family": "Watt", "given": "Ward B", "initials": "WB"}, {"family": "Martin", "given": "Arnaud", "initials": "A", "orcid": "0000-0002-5980-2249", "researcher": {"href": "https://publications.scilifelab.se/researcher/d248adda76964813a0fe5de5a84de2cd.json"}}, {"family": "Wheat", "given": "Christopher W", "initials": "CW", "orcid": "0000-0003-1863-2340", "researcher": {"href": "https://publications.scilifelab.se/researcher/7e498f04977a48c89ffcd0bae890d4cb.json"}}], "type": "journal article", "published": "2023-03-22", "journal": {"title": "Sci Adv", "issn": "2375-2548", "volume": "9", "issue": "12", "pages": "eabq3713", "issn-l": "2375-2548"}, "abstract": "Understanding the evolutionary origins and factors maintaining alternative life history strategies (ALHS) within species is a major goal of evolutionary research. While alternative alleles causing discrete ALHS are expected to purge or fix over time, one-third of the ~90 species of Colias butterflies are polymorphic for a female-limited ALHS called Alba. Whether Alba arose once, evolved in parallel, or has been exchanged among taxa is currently unknown. Using comparative genome-wide association study (GWAS) and population genomic analyses, we placed the genetic basis of Alba in time-calibrated phylogenomic framework, revealing that Alba evolved once near the base of the genus and has been subsequently maintained via introgression and balancing selection. CRISPR-Cas9 mutagenesis was then used to verify a putative cis-regulatory region of Alba, which we identified using phylogenetic foot printing. We hypothesize that this cis-regulatory region acts as a modular enhancer for the induction of the Alba ALHS, which has likely facilitated its long evolutionary persistence.", "doi": "10.1126/sciadv.abq3713", "pmid": "36947619", "labels": {"NGI Short read": "Service", "National Genomics Infrastructure": "Service", "NGI Stockholm (Genomics Production)": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC10032607"}], "notes": [], "created": "2023-10-04T12:34:51.747Z", "modified": "2023-10-19T11:53:35.081Z"}, {"entity": "publication", "iuid": "9d6ef0ad3fbc4458add397850c87d9d8", "links": {"self": {"href": "https://publications.scilifelab.se/publication/9d6ef0ad3fbc4458add397850c87d9d8.json"}, "display": {"href": "https://publications.scilifelab.se/publication/9d6ef0ad3fbc4458add397850c87d9d8"}}, "title": "A Genome for Edith's Checkerspot Butterfly: An Insect with Complex Host-Adaptive Suites and Rapid Evolutionary Responses to Environmental Changes.", "authors": [{"family": "Tunstrom", "given": "Kalle", "initials": "K", "orcid": "0000-0002-5285-1531", "researcher": {"href": "https://publications.scilifelab.se/researcher/abd0ddb97d724542b6e7c46f782f3bbd.json"}}, {"family": "Wheat", "given": "Christopher W", "initials": "CW", "orcid": "0000-0003-1863-2340", "researcher": {"href": "https://publications.scilifelab.se/researcher/7e498f04977a48c89ffcd0bae890d4cb.json"}}, {"family": "Parmesan", "given": "Camille", "initials": "C"}, {"family": "Singer", "given": "Michael C", "initials": "MC"}, {"family": "Mikheyev", "given": "Alexander S", "initials": "AS"}], "type": "journal article", "published": "2022-08-03", "journal": {"title": "Genome Biol Evol", "issn": "1759-6653", "issn-l": "1759-6653", "volume": "14", "issue": "8", "pages": null}, "abstract": "Insects have been key players in the assessments of biodiversity impacts of anthropogenically driven environmental change, including the evolutionary and ecological impacts of climate change. Populations of Edith's Checkerspot Butterfly (Euphydryas editha) adapt rapidly to diverse environmental conditions, with numerous high-impact studies documenting these dynamics over several decades. However, studies of the underlying genetic bases of these responses have been hampered by missing genomic resources, limiting the ability to connect genomic responses to environmental change. Using a combination of Oxford Nanopore long reads, haplotype merging, HiC scaffolding followed by Illumina polishing, we generated a highly contiguous and complete assembly (contigs n = 142, N50 = 21.2 Mb, total length = 607.8 Mb; BUSCOs n = 5,286, single copy complete = 97.8%, duplicated = 0.9%, fragmented = 0.3%, missing = 1.0%). A total of 98% of the assembled genome was placed into 31 chromosomes, which displayed large-scale synteny with other well-characterized lepidopteran genomes. The E. editha genome, annotation, and functional descriptions now fill a missing gap for one of the leading field-based ecological model systems in North America.", "doi": "10.1093/gbe/evac113", "pmid": "35876165", "labels": {"National Genomics Infrastructure": "Service", "NGI Short read": "Service", "NGI Stockholm (Genomics Applications)": "Service", "NGI Stockholm (Genomics Production)": "Service", "NGI Other": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC9348621"}, {"db": "pii", "key": "6649524"}], "notes": [], "created": "2022-08-19T08:38:20.953Z", "modified": "2024-01-16T13:48:35.474Z"}]}