{"entity": "researcher", "timestamp": "2026-08-15T07:36:44.629Z", "family": "Ryd\u00e9n", "given": "Mikael", "initials": "M", "orcid": "0000-0003-4785-1876", "affiliations": ["Department of Medicine (H7), Karolinska Institutet, Karolinska University Hospital Huddinge, Huddinge, Sweden. mikael.ryden@ki.se."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/ab61ac66c36749019ce5066fb1a5061e.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/ab61ac66c36749019ce5066fb1a5061e"}}, "publications": [{"entity": "publication", "iuid": "c7eea563d7774ae5aa7338fb67bc2b74", "links": {"self": {"href": "https://publications.scilifelab.se/publication/c7eea563d7774ae5aa7338fb67bc2b74.json"}, "display": {"href": "https://publications.scilifelab.se/publication/c7eea563d7774ae5aa7338fb67bc2b74"}}, "title": "Reduced adipocyte glutaminase activity promotes energy expenditure and metabolic health.", "authors": [{"family": "Lecoutre", "given": "Simon", "initials": "S", "orcid": "0000-0002-2536-6119", "researcher": {"href": "https://publications.scilifelab.se/researcher/ac1619986a25402b994efa1811b5cfff.json"}}, {"family": "Maqdasy", "given": "Salwan", "initials": "S", "orcid": "0000-0001-5164-9879", "researcher": {"href": "https://publications.scilifelab.se/researcher/809a82e9fa1e4173ba1dd2133a91d5c5.json"}}, {"family": "Rizo-Roca", "given": "David", "initials": "D", "orcid": "0000-0002-9379-7543", "researcher": {"href": "https://publications.scilifelab.se/researcher/52016d47cd9c4dcab663c166cc1afa68.json"}}, {"family": "Renzi", "given": "Gianluca", "initials": "G"}, {"family": "Vlassakev", "given": "Ivan", "initials": "I"}, {"family": "Alaeddine", "given": "Lynn M", "initials": "LM", "orcid": "0000-0001-8494-7514", "researcher": {"href": "https://publications.scilifelab.se/researcher/3d7386fee9ac4d6da8f738bb573302ac.json"}}, {"family": "Higos", "given": "Romane", "initials": "R"}, {"family": "Jalkanen", "given": "Jutta", "initials": "J", "orcid": "0000-0002-0219-1096", "researcher": {"href": "https://publications.scilifelab.se/researcher/96620636aff142c484d07894c573f3d2.json"}}, {"family": "Zhong", "given": "Jiawei", "initials": "J", "orcid": "0000-0002-2802-0770", "researcher": {"href": "https://publications.scilifelab.se/researcher/3d30cbe9680a42c1b6478921e4b63287.json"}}, {"family": "Zareifi", "given": "Danae S", "initials": "DS", "orcid": "0000-0003-2108-4955", "researcher": {"href": "https://publications.scilifelab.se/researcher/90ffdf76251f455bb8f15d9de4f0e86a.json"}}, {"family": "Frendo-Cumbo", "given": "Scott", "initials": "S", "orcid": "0000-0002-9047-4650", "researcher": {"href": "https://publications.scilifelab.se/researcher/4bfa1b27416e47fd90a77657fcb0a34e.json"}}, {"family": "Massier", "given": "Lucas", "initials": "L", "orcid": "0000-0002-6199-2643", "researcher": {"href": "https://publications.scilifelab.se/researcher/94411e29fc224eb3b8b215f984f05ee9.json"}}, {"family": "Hodek", "given": "Ondrej", "initials": "O", "orcid": "0000-0001-8307-9575", "researcher": {"href": "https://publications.scilifelab.se/researcher/0e3bcef870804d4f9ec3d3e257cbc323.json"}}, {"family": "Juvany", "given": "Marta", "initials": "M"}, {"family": "Moritz", "given": "Thomas", "initials": "T"}, {"family": "de Castro Barbosa", "given": "Thais", "initials": "T", "orcid": "0000-0002-3040-9873", "researcher": {"href": "https://publications.scilifelab.se/researcher/b8991ec28185464eaf5c8ee076b9d071.json"}}, {"family": "Omar-Hmeadi", "given": "Muhmmad", "initials": "M", "orcid": "0000-0001-8893-7348", "researcher": {"href": "https://publications.scilifelab.se/researcher/4776032637a2418682320dc68bf9417f.json"}}, {"family": "L\u00f3pez-Yus", "given": "Marta", "initials": "M", "orcid": "0000-0002-5822-9823", "researcher": {"href": "https://publications.scilifelab.se/researcher/ecc979a3cc074d69860f44f6c51a1d1b.json"}}, {"family": "Merabtene", "given": "Fatiha", "initials": "F"}, {"family": "Abatan", "given": "Jimon Boniface", "initials": "JB"}, {"family": "Marcelin", "given": "Genevi\u00e8ve", "initials": "G"}, {"family": "El Hachem", "given": "Elie-Julien", "initials": "EJ", "orcid": "0000-0001-5033-6117", "researcher": {"href": "https://publications.scilifelab.se/researcher/0862046052b9458fa7710d2263a133e2.json"}}, {"family": "Rouault", "given": "Christine", "initials": "C"}, {"family": "Bergo", "given": "Martin O", "initials": "MO"}, {"family": "Petrus", "given": "Paul", "initials": "P", "orcid": "0000-0001-8297-3874", "researcher": {"href": "https://publications.scilifelab.se/researcher/baffd8ee268542d6a3702ab97b985d14.json"}}, {"family": "Zierath", "given": "Juleen R", "initials": "JR", "orcid": "0000-0001-6891-7497", "researcher": {"href": "https://publications.scilifelab.se/researcher/f4ef2b94ba2d44dd9b41192567a7af53.json"}}, {"family": "Cl\u00e9ment", "given": "Karine", "initials": "K", "orcid": "0000-0002-2489-3355", "researcher": {"href": "https://publications.scilifelab.se/researcher/840fc2b0de56464cabf3c130dfedf57f.json"}}, {"family": "Krook", "given": "Anna", "initials": "A", "orcid": "0000-0002-0891-0258", "researcher": {"href": "https://publications.scilifelab.se/researcher/f43339bcd571486b845a3b10e241bcc4.json"}}, {"family": "Mejhert", "given": "Niklas", "initials": "N", "orcid": "0000-0003-1785-833X", "researcher": {"href": "https://publications.scilifelab.se/researcher/81eb60b84ea14ad9ba7e4d3433c37a3e.json"}}, {"family": "Ryd\u00e9n", "given": "Mikael", "initials": "M", "orcid": "0000-0003-4785-1876", "researcher": {"href": "https://publications.scilifelab.se/researcher/ab61ac66c36749019ce5066fb1a5061e.json"}}], "type": "journal article", "published": "2024-07-00", "journal": {"title": "Nat Metab", "issn": "2522-5812", "volume": "6", "issue": "7", "pages": "1329-1346", "issn-l": "2522-5812"}, "abstract": "Glutamine and glutamate are interconverted by several enzymes and alterations in this metabolic cycle are linked to cardiometabolic traits. Herein, we show that obesity-associated insulin resistance is characterized by decreased plasma and white adipose tissue glutamine-to-glutamate ratios. We couple these stoichiometric changes to perturbed fat cell glutaminase and glutamine synthase messenger RNA and protein abundance, which together promote glutaminolysis. In human white adipocytes, reductions in glutaminase activity promote aerobic glycolysis and mitochondrial oxidative capacity via increases in hypoxia-inducible factor 1\u03b1 abundance, lactate levels and p38 mitogen-activated protein kinase signalling. Systemic glutaminase inhibition in male and female mice, or genetically in adipocytes of male mice, triggers the activation of thermogenic gene programs in inguinal adipocytes. Consequently, the knockout mice display higher energy expenditure and improved glucose tolerance compared to control littermates, even under high-fat diet conditions. Altogether, our findings highlight white adipocyte glutamine turnover as an important determinant of energy expenditure and metabolic health.", "doi": "10.1038/s42255-024-01083-y", "pmid": "39009762", "labels": {"Swedish Metabolomics Centre": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC11272588"}, {"db": "pii", "key": "10.1038/s42255-024-01083-y"}], "notes": [], "created": "2024-11-26T10:26:41.024Z", "modified": "2025-10-17T13:03:13.077Z"}, {"entity": "publication", "iuid": "146c0055084542878ac0e9cb73932a95", "links": {"self": {"href": "https://publications.scilifelab.se/publication/146c0055084542878ac0e9cb73932a95.json"}, "display": {"href": "https://publications.scilifelab.se/publication/146c0055084542878ac0e9cb73932a95"}}, "title": "An integrated single cell and spatial transcriptomic map of human white adipose tissue.", "authors": [{"family": "Massier", "given": "Lucas", "initials": "L"}, {"family": "Jalkanen", "given": "Jutta", "initials": "J", "orcid": "0000-0002-0219-1096", "researcher": {"href": "https://publications.scilifelab.se/researcher/96620636aff142c484d07894c573f3d2.json"}}, {"family": "Elmastas", "given": "Merve", "initials": "M", "orcid": "0000-0002-1346-5080", "researcher": {"href": "https://publications.scilifelab.se/researcher/a4055754f8cb4035ac1e160cc3cad356.json"}}, {"family": "Zhong", "given": "Jiawei", "initials": "J", "orcid": "0000-0002-2802-0770", "researcher": {"href": "https://publications.scilifelab.se/researcher/3d30cbe9680a42c1b6478921e4b63287.json"}}, {"family": "Wang", "given": "Tongtong", "initials": "T"}, {"family": "Nono Nankam", "given": "Pamela A", "initials": "PA", "orcid": "0000-0001-9301-4556", "researcher": {"href": "https://publications.scilifelab.se/researcher/ff19be55f9dd48f6becb1a0c47000d68.json"}}, {"family": "Frendo-Cumbo", "given": "Scott", "initials": "S", "orcid": "0000-0002-9047-4650", "researcher": {"href": "https://publications.scilifelab.se/researcher/4bfa1b27416e47fd90a77657fcb0a34e.json"}}, {"family": "B\u00e4ckdahl", "given": "Jesper", "initials": "J"}, {"family": "Subramanian", "given": "Narmadha", "initials": "N"}, {"family": "Sekine", "given": "Takuya", "initials": "T"}, {"family": "Kerr", "given": "Alastair G", "initials": "AG", "orcid": "0000-0003-2085-1542", "researcher": {"href": "https://publications.scilifelab.se/researcher/076ba1b03b78451ba1ddb95b04e74b27.json"}}, {"family": "Tseng", "given": "Ben T P", "initials": "BTP"}, {"family": "Laurencikiene", "given": "Jurga", "initials": "J"}, {"family": "Buggert", "given": "Marcus", "initials": "M", "orcid": "0000-0003-0633-1719", "researcher": {"href": "https://publications.scilifelab.se/researcher/9a54e4b5136642eeafa77ac5118a0c81.json"}}, {"family": "Lourda", "given": "Magda", "initials": "M", "orcid": "0000-0003-3155-1123", "researcher": {"href": "https://publications.scilifelab.se/researcher/64d9a0bc6b5d4039af5bc308a97eaad6.json"}}, {"family": "Kublickiene", "given": "Karolina", "initials": "K", "orcid": "0000-0002-4841-6836", "researcher": {"href": "https://publications.scilifelab.se/researcher/42acd99d04974333989f148a1f960207.json"}}, {"family": "Bhalla", "given": "Nayanika", "initials": "N", "orcid": "0000-0002-6800-0432", "researcher": {"href": "https://publications.scilifelab.se/researcher/add89d1454ce490ab138768993411d70.json"}}, {"family": "Andersson", "given": "Alma", "initials": "A"}, {"family": "Valsesia", "given": "Armand", "initials": "A", "orcid": "0000-0003-0746-9664", "researcher": {"href": "https://publications.scilifelab.se/researcher/b480c6022f2f43bdb95e7a37e11d5235.json"}}, {"family": "Astrup", "given": "Arne", "initials": "A", "orcid": "0000-0001-8968-8996", "researcher": {"href": "https://publications.scilifelab.se/researcher/aa3d74e15326474282c62b26c4c1507b.json"}}, {"family": "Blaak", "given": "Ellen E", "initials": "EE", "orcid": "0000-0002-2496-3464", "researcher": {"href": "https://publications.scilifelab.se/researcher/4fa433d6fe8d4b6b811c20a88378e4eb.json"}}, {"family": "St\u00e5hl", "given": "Patrik L", "initials": "PL", "orcid": "0000-0002-2207-7370", "researcher": {"href": "https://publications.scilifelab.se/researcher/6ea50cf03c6748c086846c3a28882979.json"}}, {"family": "Viguerie", "given": "Nathalie", "initials": "N", "orcid": "0000-0002-1730-9915", "researcher": {"href": "https://publications.scilifelab.se/researcher/a1b76daf0c9e478b974d0fa9fb4aa5a1.json"}}, {"family": "Langin", "given": "Dominique", "initials": "D", "orcid": "0000-0002-2669-7825", "researcher": {"href": "https://publications.scilifelab.se/researcher/689d03a8a3614168bc04d897a7cfd0c2.json"}}, {"family": "Wolfrum", "given": "Christian", "initials": "C", "orcid": "0000-0002-3862-6805", "researcher": {"href": "https://publications.scilifelab.se/researcher/43777b7ae29b470793e30b6d32d2674f.json"}}, {"family": "Bl\u00fcher", "given": "Matthias", "initials": "M"}, {"family": "Ryd\u00e9n", "given": "Mikael", "initials": "M", "orcid": "0000-0003-4785-1876", "researcher": {"href": "https://publications.scilifelab.se/researcher/ab61ac66c36749019ce5066fb1a5061e.json"}}, {"family": "Mejhert", "given": "Niklas", "initials": "N", "orcid": "0000-0003-1785-833X", "researcher": {"href": "https://publications.scilifelab.se/researcher/81eb60b84ea14ad9ba7e4d3433c37a3e.json"}}], "type": "meta-analysis", "published": "2023-03-15", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "14", "issue": "1", "pages": "1438", "issn-l": "2041-1723"}, "abstract": "To date, single-cell studies of human white adipose tissue (WAT) have been based on small cohort sizes and no cellular consensus nomenclature exists. Herein, we performed a comprehensive meta-analysis of publicly available and newly generated single-cell, single-nucleus, and spatial transcriptomic results from human subcutaneous, omental, and perivascular WAT. Our high-resolution map is built on data from ten studies and allowed us to robustly identify >60 subpopulations of adipocytes, fibroblast and adipogenic progenitors, vascular, and immune cells. Using these results, we deconvolved spatial and bulk transcriptomic data from nine additional cohorts to provide spatial and clinical dimensions to the map. This identified cell-cell interactions as well as relationships between specific cell subtypes and insulin resistance, dyslipidemia, adipocyte volume, and lipolysis upon long-term weight changes. Altogether, our meta-map provides a rich resource defining the cellular and microarchitectural landscape of human WAT and describes the associations between specific cell types and metabolic states.", "doi": "10.1038/s41467-023-36983-2", "pmid": "36922516", "labels": {"NGI Short read": "Service", "National Genomics Infrastructure": "Service", "NGI Stockholm (Genomics Production)": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC10017705"}, {"db": "pii", "key": "10.1038/s41467-023-36983-2"}], "notes": [], "created": "2023-10-04T12:19:09.279Z", "modified": "2023-10-19T10:08:08.367Z"}, {"entity": "publication", "iuid": "17cbfb54c2314acab08b8b2221e6e82f", "links": {"self": {"href": "https://publications.scilifelab.se/publication/17cbfb54c2314acab08b8b2221e6e82f.json"}, "display": {"href": "https://publications.scilifelab.se/publication/17cbfb54c2314acab08b8b2221e6e82f"}}, "title": "Impaired phosphocreatine metabolism in white adipocytes promotes inflammation.", "authors": [{"family": "Maqdasy", "given": "Salwan", "initials": "S", "orcid": "0000-0001-5164-9879", "researcher": {"href": "https://publications.scilifelab.se/researcher/809a82e9fa1e4173ba1dd2133a91d5c5.json"}}, {"family": "Lecoutre", "given": "Simon", "initials": "S", "orcid": "0000-0002-2536-6119", "researcher": {"href": "https://publications.scilifelab.se/researcher/ac1619986a25402b994efa1811b5cfff.json"}}, {"family": "Renzi", "given": "Gianluca", "initials": "G"}, {"family": "Frendo-Cumbo", "given": "Scott", "initials": "S", "orcid": "0000-0002-9047-4650", "researcher": {"href": "https://publications.scilifelab.se/researcher/4bfa1b27416e47fd90a77657fcb0a34e.json"}}, {"family": "Rizo-Roca", "given": "David", "initials": "D"}, {"family": "Moritz", "given": "Thomas", "initials": "T", "orcid": "0000-0002-4258-3190", "researcher": {"href": "https://publications.scilifelab.se/researcher/95ad5b7fe48f42eda1328f54a385e097.json"}}, {"family": "Juvany", "given": "Marta", "initials": "M"}, {"family": "Hodek", "given": "Ondrej", "initials": "O"}, {"family": "Gao", "given": "Hui", "initials": "H", "orcid": "0000-0002-7306-7471", "researcher": {"href": "https://publications.scilifelab.se/researcher/c212c77475f042e284e0cbe9ae4c38fa.json"}}, {"family": "Couchet", "given": "Morgane", "initials": "M"}, {"family": "Witting", "given": "Michael", "initials": "M", "orcid": "0000-0002-1462-4426", "researcher": {"href": "https://publications.scilifelab.se/researcher/b522a40ff2e84e76a4e6d83fa1e8ff0a.json"}}, {"family": "Kerr", "given": "Alastair", "initials": "A", "orcid": "0000-0003-2085-1542", "researcher": {"href": "https://publications.scilifelab.se/researcher/076ba1b03b78451ba1ddb95b04e74b27.json"}}, {"family": "Bergo", "given": "Martin O", "initials": "MO"}, {"family": "Choudhury", "given": "Robin P", "initials": "RP"}, {"family": "Aouadi", "given": "Myriam", "initials": "M", "orcid": "0000-0001-6256-7107", "researcher": {"href": "https://publications.scilifelab.se/researcher/7ea7bb2be61a4e818685466d1005659d.json"}}, {"family": "Zierath", "given": "Juleen R", "initials": "JR", "orcid": "0000-0001-6891-7497", "researcher": {"href": "https://publications.scilifelab.se/researcher/f4ef2b94ba2d44dd9b41192567a7af53.json"}}, {"family": "Krook", "given": "Anna", "initials": "A", "orcid": "0000-0002-0891-0258", "researcher": {"href": "https://publications.scilifelab.se/researcher/f43339bcd571486b845a3b10e241bcc4.json"}}, {"family": "Mejhert", "given": "Niklas", "initials": "N", "orcid": "0000-0003-1785-833X", "researcher": {"href": "https://publications.scilifelab.se/researcher/81eb60b84ea14ad9ba7e4d3433c37a3e.json"}}, {"family": "Ryd\u00e9n", "given": "Mikael", "initials": "M", "orcid": "0000-0003-4785-1876", "researcher": {"href": "https://publications.scilifelab.se/researcher/ab61ac66c36749019ce5066fb1a5061e.json"}}], "type": "journal article", "published": "2022-02-00", "journal": {"title": "Nat Metab", "issn": "2522-5812", "volume": "4", "issue": "2", "pages": "190-202", "issn-l": "2522-5812"}, "abstract": "The mechanisms promoting disturbed white adipocyte function in obesity remain largely unclear. Herein, we integrate white adipose tissue (WAT) metabolomic and transcriptomic data from clinical cohorts and find that the WAT phosphocreatine/creatine ratio is increased and creatine kinase-B expression and activity is decreased in the obese state. In human in vitro and murine in vivo models, we demonstrate that decreased phosphocreatine metabolism in white adipocytes alters adenosine monophosphate-activated protein kinase activity via effects on adenosine triphosphate/adenosine diphosphate levels, independently of WAT beigeing. This disturbance promotes a pro-inflammatory profile characterized, in part, by increased chemokine (C-C motif) ligand 2 (CCL2) production. These data suggest that the phosphocreatine/creatine system links cellular energy shuttling with pro-inflammatory responses in human and murine white adipocytes. Our findings provide unexpected perspectives on the mechanisms driving WAT inflammation in obesity and may present avenues to target adipocyte dysfunction.", "doi": "10.1038/s42255-022-00525-9", "pmid": "35165448", "labels": {"Swedish Metabolomics Centre": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC8885409"}, {"db": "pii", "key": "10.1038/s42255-022-00525-9"}], "notes": [], "created": "2022-12-05T08:10:40.997Z", "modified": "2025-10-17T13:03:15.360Z"}]}