{"entity": "researcher", "timestamp": "2026-07-20T01:40:38.237Z", "family": "Skalick\u00fd", "given": "Vladim\u00edr", "initials": "V", "orcid": "0000-0001-5369-8906", "affiliations": ["Laboratory of Growth Regulators, Faculty of Science, Palack\u00fd University and Institute of Experimental Botany of the Czech Academy of Sciences, CZ-78371, Olomouc, Czech Republic."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/aa0883baaa234b1eb8f80b6b5dc2ac07.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/aa0883baaa234b1eb8f80b6b5dc2ac07"}}, "publications": [{"entity": "publication", "iuid": "6f47d65ab69d4679a11ed59bb80b8255", "links": {"self": {"href": "https://publications.scilifelab.se/publication/6f47d65ab69d4679a11ed59bb80b8255.json"}, "display": {"href": "https://publications.scilifelab.se/publication/6f47d65ab69d4679a11ed59bb80b8255"}}, "title": "Apoptotic bodies in phytoplankton suggest evolutionary conservation of cell death mechanisms.", "authors": [{"family": "Corredor", "given": "Luisa", "initials": "L", "orcid": "0000-0003-0274-4962", "researcher": {"href": "https://publications.scilifelab.se/researcher/1b51b1c56fdc49fbb0596c6ca8448315.json"}}, {"family": "Vergou", "given": "Georgia Antonia", "initials": "GA", "orcid": "0009-0004-0913-7203", "researcher": {"href": "https://publications.scilifelab.se/researcher/fe3d2255d757451a8565911e9924486c.json"}}, {"family": "Skalick\u00fd", "given": "Vladim\u00edr", "initials": "V", "orcid": "0000-0001-5369-8906", "researcher": {"href": "https://publications.scilifelab.se/researcher/aa0883baaa234b1eb8f80b6b5dc2ac07.json"}}, {"family": "Antoniadi", "given": "Ioanna", "initials": "I", "orcid": "0000-0001-9053-2788", "researcher": {"href": "https://publications.scilifelab.se/researcher/60bcbb474ded4df08079520c92de49bf.json"}}, {"family": "Wheaton", "given": "Benjamin J", "initials": "BJ", "orcid": "0000-0003-0619-4777", "researcher": {"href": "https://publications.scilifelab.se/researcher/399d29926ef74784aeda6c6ada5cedb4.json"}}, {"family": "Ljung", "given": "Karin", "initials": "K", "orcid": "0000-0003-2901-189X", "researcher": {"href": "https://publications.scilifelab.se/researcher/f91b1e1f90c24559b915ebcd265804a4.json"}}, {"family": "Gorzs\u00e1s", "given": "Andr\u00e1s", "initials": "A", "orcid": "0000-0002-2298-8844", "researcher": {"href": "https://publications.scilifelab.se/researcher/8070792ccead4c809c89943d84cf0e03.json"}}, {"family": "Funk", "given": "Christiane", "initials": "C", "orcid": "0000-0002-7897-4038", "researcher": {"href": "https://publications.scilifelab.se/researcher/5db6c5fa2a754060b1583caf37bba1b8.json"}}], "type": "journal article", "published": "2025-09-25", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "16", "issue": "1", "pages": "8427", "issn-l": "2041-1723"}, "abstract": "Programmed Cell Death (PCD) in eukaryotes is a regulated process occurring during development, cell differentiation and aging. Apoptosis is a particularly well studied morphotype of PCD, only observed in animal cells (metazoan). Its most definitive hallmark is the formation and release of membrane-enclosed extracellular vesicles called Apoptotic Bodies (ABs). Although apoptotic-like features have been described in plants, yeast, protozoa and phytoplankton, the production of ABs has been thought to be limited to multicellular animals. Here we report the production and release of extracellular ABs in a non-metazoan unicellular eukaryote, the cryptophyte alga Guillardia theta. Morphologies of G. theta cells during aging and pharmacologically-induced cell death confirm the presence of ABs and apoptosis in phytoplankton. G. theta ABs have similar composition to metazoan ABs, carrying DNA, proteins, lipids, carbohydrates, fragments of organelles and cytosol portions. Our results demonstrate that G. theta, a microalga that arose from secondary endosymbiosis, experiences apoptotic cell death in physiological conditions, similar to animal cells. Since secondary endosymbiosis occurred prior to the origin of multicellularity, our discovery questions the evolutionary origin of PCD.", "doi": "10.1038/s41467-025-63956-4", "pmid": "40998840", "labels": {"Cryo-EM": "Service", "Integrated Microscopy Technologies Ume\u00e5": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC12462507"}, {"db": "pii", "key": "10.1038/s41467-025-63956-4"}], "notes": [], "created": "2025-09-30T12:06:57.996Z", "modified": "2025-10-30T12:08:13.065Z"}, {"entity": "publication", "iuid": "a5f8668f28494e83a69d5f25e8db3fde", "links": {"self": {"href": "https://publications.scilifelab.se/publication/a5f8668f28494e83a69d5f25e8db3fde.json"}, "display": {"href": "https://publications.scilifelab.se/publication/a5f8668f28494e83a69d5f25e8db3fde"}}, "title": "Fluorescence-activated multi-organelle mapping of subcellular plant hormone distribution.", "authors": [{"family": "Skalick\u00fd", "given": "Vladim\u00edr", "initials": "V", "orcid": "0000-0001-5369-8906", "researcher": {"href": "https://publications.scilifelab.se/researcher/aa0883baaa234b1eb8f80b6b5dc2ac07.json"}}, {"family": "Antoniadi", "given": "Ioanna", "initials": "I", "orcid": "0000-0001-9053-2788", "researcher": {"href": "https://publications.scilifelab.se/researcher/60bcbb474ded4df08079520c92de49bf.json"}}, {"family": "P\u011bn\u010d\u00edk", "given": "Ale\u0161", "initials": "A", "orcid": "0000-0002-1314-2249", "researcher": {"href": "https://publications.scilifelab.se/researcher/b13dbbec35c441daa7ed84daa05cd6e7.json"}}, {"family": "Chamr\u00e1d", "given": "Ivo", "initials": "I", "orcid": "0000-0003-3812-6314", "researcher": {"href": "https://publications.scilifelab.se/researcher/83a3faec745445f0a9b0b0d2cd7538cb.json"}}, {"family": "Lenobel", "given": "Ren\u00e9", "initials": "R", "orcid": "0000-0001-7387-0056", "researcher": {"href": "https://publications.scilifelab.se/researcher/1283e2c432974d159fe2b24c4fa9f48f.json"}}, {"family": "Kube\u0161", "given": "Martin F", "initials": "MF", "orcid": "0000-0003-2182-4490", "researcher": {"href": "https://publications.scilifelab.se/researcher/e50ec82af0434dbd8b159dc6bbd7f1c6.json"}}, {"family": "Zatloukal", "given": "Marek", "initials": "M", "orcid": "0000-0001-9962-2798", "researcher": {"href": "https://publications.scilifelab.se/researcher/aa91c54a8d7e413b8bb581dae3a11556.json"}}, {"family": "\u017dukauskait\u0117", "given": "Asta", "initials": "A", "orcid": "0000-0001-6759-8789", "researcher": {"href": "https://publications.scilifelab.se/researcher/d2f55d830fe14a6db1f912a852666646.json"}}, {"family": "Strnad", "given": "Miroslav", "initials": "M", "orcid": "0000-0002-2806-794X", "researcher": {"href": "https://publications.scilifelab.se/researcher/141c6516a148415c8d089a5b2ce40f75.json"}}, {"family": "Ljung", "given": "Karin", "initials": "K", "orcid": "0000-0003-2901-189X", "researcher": {"href": "https://publications.scilifelab.se/researcher/f91b1e1f90c24559b915ebcd265804a4.json"}}, {"family": "Nov\u00e1k", "given": "Ond\u0159ej", "initials": "O", "orcid": "0000-0003-3452-0154", "researcher": {"href": "https://publications.scilifelab.se/researcher/8c19165acb9a4ff79dd96af7fccdc5f8.json"}}], "type": "journal article", "published": "2023-12-00", "journal": {"title": "Plant J.", "issn": "1365-313X", "volume": "116", "issue": "6", "pages": "1825-1841", "issn-l": "0960-7412"}, "abstract": "Auxins and cytokinins are two major families of phytohormones that control most aspects of plant growth, development and plasticity. Their distribution in plants has been described, but the importance of cell- and subcellular-type specific phytohormone homeostasis remains undefined. Herein, we revealed auxin and cytokinin distribution maps showing their different organelle-specific allocations within the Arabidopsis plant cell. To do so, we have developed Fluorescence-Activated multi-Organelle Sorting (FAmOS), an innovative subcellular fractionation technique based on flow cytometric principles. FAmOS allows the simultaneous sorting of four differently labelled organelles based on their individual light scatter and fluorescence parameters while ensuring hormone metabolic stability. Our data showed different subcellular distribution of auxin and cytokinins, revealing the formation of phytohormone gradients that have been suggested by the subcellular localization of auxin and cytokinin transporters, receptors and metabolic enzymes. Both hormones showed enrichment in vacuoles, while cytokinins were also accumulated in the endoplasmic reticulum.", "doi": "10.1111/tpj.16456", "pmid": "37682018", "labels": {"Swedish Metabolomics Centre": "Service"}, "xrefs": [], "notes": [], "created": "2024-01-18T14:45:38.272Z", "modified": "2025-10-17T13:03:13.273Z"}]}