{"entity": "researcher", "timestamp": "2026-08-17T02:29:52.733Z", "family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "affiliations": ["Division of Micro and Nanosystems, KTH Royal Institute of Technology, Malvinas v\u00e4g 10, Stockholm, 100 44, Sweden.", "AIMES - Center for the Advancement of Integrated Medical and Engineering Sciences, Department of Neuroscience, Karolinska Institute, Stockholm, 17177, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/a9d4508938be49ddabacb3a1f39969d4.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/a9d4508938be49ddabacb3a1f39969d4"}}, "publications": [{"entity": "publication", "iuid": "f992edd214ec4d1582d8ac65d305d0a3", "links": {"self": {"href": "https://publications.scilifelab.se/publication/f992edd214ec4d1582d8ac65d305d0a3.json"}, "display": {"href": "https://publications.scilifelab.se/publication/f992edd214ec4d1582d8ac65d305d0a3"}}, "title": "Generation of Human iPSC-Derived Astrocytes with a mature star-shaped phenotype for CNS modeling.", "authors": [{"family": "Voulgaris", "given": "Dimitrios", "initials": "D"}, {"family": "Nikolakopoulou", "given": "Polyxeni", "initials": "P"}, {"family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "researcher": {"href": "https://publications.scilifelab.se/researcher/a9d4508938be49ddabacb3a1f39969d4.json"}}], "type": "journal article", "published": "2022-10-00", "journal": {"title": "Stem Cell Rev Rep", "issn": "2629-3277", "volume": "18", "issue": "7", "pages": "2494-2512", "issn-l": null}, "abstract": "The generation of astrocytes from human induced pluripotent stem cells has been hampered by either prolonged differentiation-spanning over two months-or by shorter protocols that generate immature astrocytes, devoid of salient mature astrocytic traits pivotal for central nervous system (CNS) modeling. We directed stable hiPSC-derived neuroepithelial stem cells to human iPSC-derived Astrocytes (hiAstrocytes) with a high percentage of star-shaped cells by orchestrating an astrocytic-tuned culturing environment in 28 days. We employed RT-qPCR and ICC to validate the astrocytic commitment of the neuroepithelial stem cells. To evaluate the inflammatory phenotype, we challenged the hiAstrocytes with the pro-inflammatory cytokine IL-1\u03b2 (interleukin 1 beta) and quantitatively assessed the secretion profile of astrocyte-associated cytokines and the expression of intercellular adhesion molecule 1 (ICAM-1). Finally, we quantitatively assessed the capacity of hiAstrocytes to synthesize and export the antioxidant glutathione. In under 28 days, the generated cells express canonical and mature astrocytic markers, denoted by the expression of GFAP, AQP4 and ALDH1L1. In addition, the notion of a mature phenotype is reinforced by the expression of both astrocytic glutamate transporters EAAT1 and EAAT2. Thus, hiAstrocytes have a mature phenotype that encompasses traits critical in CNS modeling, including glutathione synthesis and secretion, upregulation of ICAM-1 and a cytokine secretion profile on a par with human fetal astrocytes. This protocol generates a multifaceted astrocytic model suitable for in vitro CNS disease modeling and personalized medicine.", "doi": "10.1007/s12015-022-10376-2", "pmid": "35488987", "labels": {"Affinity Proteomics Uppsala": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC9489586"}, {"db": "pii", "key": "10.1007/s12015-022-10376-2"}], "notes": [], "created": "2022-12-02T11:21:49.530Z", "modified": "2022-12-02T11:21:49.558Z"}, {"entity": "publication", "iuid": "28fbca31a083484e900428034205c04c", "links": {"self": {"href": "https://publications.scilifelab.se/publication/28fbca31a083484e900428034205c04c.json"}, "display": {"href": "https://publications.scilifelab.se/publication/28fbca31a083484e900428034205c04c"}}, "title": "3D Microvascularized Tissue Models by Laser-Based Cavitation Molding of Collagen.", "authors": [{"family": "Enrico", "given": "Alessandro", "initials": "A", "orcid": "0000-0002-8821-6759", "researcher": {"href": "https://publications.scilifelab.se/researcher/b30502c4fa3449cc9c24beb008784119.json"}}, {"family": "Voulgaris", "given": "Dimitrios", "initials": "D", "orcid": "0000-0003-4574-1702", "researcher": {"href": "https://publications.scilifelab.se/researcher/cbe852335b244342ba06b855e4135205.json"}}, {"family": "\u00d6stmans", "given": "Rebecca", "initials": "R"}, {"family": "Sundaravadivel", "given": "Naveen", "initials": "N"}, {"family": "Moutaux", "given": "Lucille", "initials": "L"}, {"family": "Cordier", "given": "Aur\u00e9lie", "initials": "A"}, {"family": "Niklaus", "given": "Frank", "initials": "F", "orcid": "0000-0002-0525-8647", "researcher": {"href": "https://publications.scilifelab.se/researcher/b001b91a6d9a4ec0aafb93bdd5e3be17.json"}}, {"family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "researcher": {"href": "https://publications.scilifelab.se/researcher/a9d4508938be49ddabacb3a1f39969d4.json"}}, {"family": "Stemme", "given": "G\u00f6ran", "initials": "G", "orcid": "0000-0001-9552-4234", "researcher": {"href": "https://publications.scilifelab.se/researcher/446ac390fcad4ced98cd8d7c89e1d4da.json"}}], "type": "journal article", "published": "2022-01-14", "journal": {"title": "Adv Mater", "issn": "1521-4095", "pages": "e2109823", "issn-l": null}, "abstract": "3D tissue models recapitulating human physiology are important for fundamental biomedical research, and they hold promise to become a new tool in drug development. An integrated and defined microvasculature in 3D tissue models is necessary for optimal cell functions. However, conventional bioprinting only allows the fabrication of hydrogel scaffolds containing vessel-like structures with large diameters (>100 \u00b5m) and simple geometries. Recent developments in laser photoablation enable the generation of this type of structure with higher resolution and complexity, but the photo-thermal process can compromise cell viability and hydrogel integrity. To address these limitations, the present work reports in situ 3D patterning of collagen hydrogels by femtosecond laser irradiation to create channels and cavities with diameters ranging from 20 to 60 \u00b5m. In this process, laser irradiation of the hydrogel generates cavitation gas bubbles that rearrange the collagen fibers, thereby creating stable microchannels. Such 3D channels can be formed in cell- and organoid-laden hydrogel without affecting the viability outside the lumen and can enable the formation of artificial microvasculature by the culture of endothelial cells and cell media perfusion. Thus, this method enables organs-on-a-chip and 3D tissue models featuring complex microvasculature.", "doi": "10.1002/adma.202109823", "pmid": "35029309", "labels": {"Integrated Microscopy Technologies Stockholm": "Service"}, "xrefs": [], "notes": [], "created": "2022-02-16T15:24:31.224Z", "modified": "2022-02-16T15:24:31.439Z"}]}