{"entity": "researcher", "timestamp": "2026-07-11T05:03:31.997Z", "family": "H\u00f6gelin", "given": "Emil Rydell", "initials": "ER", "orcid": "0000-0002-0155-6655", "affiliations": ["Department of Medicine and Geriatrics, Region J\u00f6nk\u00f6ping County, Eksj\u00f6, Sweden.", "Department of Biomedical and Clinical Sciences, Link\u00f6ping University, Link\u00f6ping, Sweden.", "Division of Pediatric Neurology, Department of Women's and Children's Health, Karolinska Institute, Stockholm, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/a797176a0ef44f38b35727870aa42099.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/a797176a0ef44f38b35727870aa42099"}}, "publications": [{"entity": "publication", "iuid": "0a1bc06aed8944028dbc6a1adb526828", "links": {"self": {"href": "https://publications.scilifelab.se/publication/0a1bc06aed8944028dbc6a1adb526828.json"}, "display": {"href": "https://publications.scilifelab.se/publication/0a1bc06aed8944028dbc6a1adb526828"}}, "title": "Skeletal Muscle Microbiopsies in Children and Adults-Tolerability, Sample Yield, and Analyzability.", "authors": [{"family": "H\u00f6gelin", "given": "Emil Rydell", "initials": "ER", "orcid": "0000-0002-0155-6655", "researcher": {"href": "https://publications.scilifelab.se/researcher/a797176a0ef44f38b35727870aa42099.json"}}, {"family": "Edman", "given": "Sebastian", "initials": "S"}, {"family": "Jannig", "given": "Paulo R", "initials": "PR"}, {"family": "L\u00f6fgren", "given": "Axel", "initials": "A"}, {"family": "Thulin", "given": "Kajsa", "initials": "K"}, {"family": "Michno", "given": "Piotr", "initials": "P"}, {"family": "Norrbom", "given": "Jessica", "initials": "J"}, {"family": "Alkner", "given": "Bj\u00f6rn A", "initials": "BA"}, {"family": "von Walden", "given": "Ferdinand", "initials": "F", "orcid": "0000-0003-1134-2252", "researcher": {"href": "https://publications.scilifelab.se/researcher/903e0b7523da4a49960e677009942f67.json"}}, {"family": "Fornander", "given": "Lotta", "initials": "L", "orcid": "0000-0003-2077-8445", "researcher": {"href": "https://publications.scilifelab.se/researcher/1bceecb024cd4adeb8032e41449c7a36.json"}}], "type": "journal article", "published": "2026-01-30", "journal": {"title": "Muscle Nerve", "issn": "1097-4598", "issn-l": null}, "abstract": "Traditional methods of sampling skeletal muscle tissue are invasive. This study aimed to evaluate a sub-millimeter core-biopsy (microbiopsy) as a potentially more tolerable method, with further regard to tissue yield and analyzability of RNA expression.\n\nChildren (9-13 years, n = 11) and adults (18-50 years, n = 16) were recruited. Microbiopsy and venipuncture were performed, with prior application of local anesthesia cream. Additionally, adults underwent a Bergstr\u00f6m muscle biopsy, with infiltrative local anesthesia. Pain was rated using the visual analog scale (VAS), reported as medians (95% CI). Microbiopsy samples were freeze-dried and weighed. To evaluate RNA sequencing performance at low tissue sample weights, a six-step incremental tissue ladder (10-500 \u03bcg) was analyzed.\n\nChildren rated venipunctures and microbiopsies low, at VAS = 0.1 (0.0-0.6) and 1.6 (0.9-3.9), respectively. Microbiopsy pain ratings were slightly higher than venipuncture, p < 0.001. Pain ratings in adults were 0.0 (0.0-0.5), 1.8 (1.3-2.4), 2.9 (2.4-3.8), and 2.7 (2.2-3.8) for venipuncture, microbiopsy, Bergstr\u00f6m biopsy, and infiltrative local anesthesia, respectively. Microbiopsy was rated less painful than Bergstr\u00f6m biopsy and local anesthesia (p < 0.05). Children did not rate microbiopsy more painful than adults (p = 0.82). Microbiopsies yielded on average 303 (SD 121.8) \u03bcg. RNA sequencing detected similar transcriptomic signatures across the tissue ladder.\n\nThe generally low pain ratings for the microbiopsy procedure support its use as a tolerable method of acquiring skeletal muscle samples in both children and adults. It represents a less painful alternative to Bergstr\u00f6m biopsies while still rendering adequate material for RNA sequencing.", "doi": "10.1002/mus.70161", "pmid": "41618578", "labels": {"National Genomics Infrastructure": "Service", "NGI Stockholm (Genomics Production)": "Service", "NGI Short read": "Service"}, "xrefs": [], "notes": [], "created": "2026-02-09T12:51:02.135Z", "modified": "2026-02-09T12:51:03.068Z"}]}