{"entity": "researcher", "timestamp": "2026-11-08T01:46:02.087Z", "family": "Land", "given": "Henrik", "initials": "H", "orcid": "0000-0003-3073-5641", "affiliations": ["Department of Chemistry - \u00c5ngstr\u00f6m Laboratory, Uppsala University, Uppsala, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/a73a6c9bd00b411d8127cc72a6ac78d1.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/a73a6c9bd00b411d8127cc72a6ac78d1"}}, "publications": [{"entity": "publication", "iuid": "1d386ae4e42446aa8ac9098ad1a0852a", "links": {"self": {"href": "https://publications.scilifelab.se/publication/1d386ae4e42446aa8ac9098ad1a0852a.json"}, "display": {"href": "https://publications.scilifelab.se/publication/1d386ae4e42446aa8ac9098ad1a0852a"}}, "title": "Beyond Canonical CO Oxidation: Structural and Evolutionary Insights Into a Non-Canonical Carbon Monoxide Dehydrogenase.", "authors": [{"family": "B\u00f6hm", "given": "Maximilian", "initials": "M", "orcid": "0000-0003-0205-8030", "researcher": {"href": "https://publications.scilifelab.se/researcher/cafd59c4b0a247468fedeac8da3d3bd5.json"}}, {"family": "Srinivas", "given": "Vivek", "initials": "V", "orcid": "0000-0002-0265-1873", "researcher": {"href": "https://publications.scilifelab.se/researcher/a6a2692ce1ff4d07ba13f6a180e9232e.json"}}, {"family": "Wiseman", "given": "Benjamin", "initials": "B", "orcid": "0000-0002-2994-5839", "researcher": {"href": "https://publications.scilifelab.se/researcher/2098646fa9de4dbc8f7cca8aeabad319.json"}}, {"family": "Huang", "given": "Ping", "initials": "P", "orcid": "0000-0002-7676-6905", "researcher": {"href": "https://publications.scilifelab.se/researcher/f6e89aa29dfb4e06bd4c1530183dd8fb.json"}}, {"family": "Senger", "given": "Moritz", "initials": "M", "orcid": "0000-0001-9225-4910", "researcher": {"href": "https://publications.scilifelab.se/researcher/da88e9696aa94341a7654e52a30604a2.json"}}, {"family": "H\u00f6gbom", "given": "Martin", "initials": "M", "orcid": "0000-0001-5574-9383", "researcher": {"href": "https://publications.scilifelab.se/researcher/d968fcccace74b689185bb9fa2d33bf8.json"}}, {"family": "Land", "given": "Henrik", "initials": "H", "orcid": "0000-0003-3073-5641", "researcher": {"href": "https://publications.scilifelab.se/researcher/a73a6c9bd00b411d8127cc72a6ac78d1.json"}}], "type": "journal article", "published": "2026-09-21", "journal": {"title": "Angew. Chem. Int. Ed. Engl.", "issn": "1521-3773", "volume": "65", "issue": "39", "pages": "e1702233", "issn-l": "1433-7851"}, "abstract": "Carbon monoxide dehydrogenases (CODHs) catalyse the reversible oxidation of CO to CO2 and play central roles in microbial carbon metabolism. While well-characterised CODHs from different phylogenetic backgrounds exhibit high bidirectional activity, the enigmatic clade B remains functionally uncharacterised. Here, we present the first structural and biochemical characterisation of a clade B CODH from Ruminococcus flavefaciens (RfCODH). It reveals striking divergence from canonical enzymes. A new anaerobic cryo-EM workflow was developed, carried out entirely under anoxic conditions by manual blotting and plunge freezing. It resulted in a 2.53 \u00c5 RfCODH structure. The structure adopts the typical CODH fold, but exhibits blocked gas channels, a compromised proton transfer pathway and disrupted cofactor coordination. This provides a structural rationale for RfCODH's severely attenuated CO oxidation activity (13 mU/mg vs. 900 U/mg for the well-studied ChCODH-II). EPR spectroscopy reveals unique oxidised C-cluster states not previously characterised in CODHs. Mirror tree analysis hints to co-evolution between clade B CODHs and associated ABC transporter substrate-binding proteins, suggesting these enzymes function in metabolism of substrates imported via the ABC transporter module. All findings indicate evolutionary repurposing of the CODH scaffold for alternative physiological functions.", "doi": "10.1002/anie.1702233", "pmid": "42495917", "labels": {"Cryo-EM": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC13592312"}], "notes": [], "created": "2026-11-05T14:00:04.751Z", "modified": "2026-11-05T14:00:04.864Z"}]}