{"entity": "researcher", "timestamp": "2026-07-20T13:37:54.807Z", "family": "Sandin", "given": "Sara", "initials": "S", "orcid": "0000-0001-6071-2761", "affiliations": ["School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.", "NTU Institute of Structural Biology, Nanyang Technological University, 59 Nanyang Drive, Singapore 636921, Singapore."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/a52c9c37f07b465781e64958a0edb780.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/a52c9c37f07b465781e64958a0edb780"}}, "publications": [{"entity": "publication", "iuid": "3cc6abe50acb4e6490f16794c487bd1b", "links": {"self": {"href": "https://publications.scilifelab.se/publication/3cc6abe50acb4e6490f16794c487bd1b.json"}, "display": {"href": "https://publications.scilifelab.se/publication/3cc6abe50acb4e6490f16794c487bd1b"}}, "title": "Uromodulin velcro sheets and their interaction with uropathogenic E. coli", "authors": [{"family": "Banjara", "given": "Suresh", "initials": "S", "orcid": "0000-0002-2123-8101", "researcher": {"href": "https://publications.scilifelab.se/researcher/b935b5b29c77445aa2e9e4ccd76afd4c.json"}}, {"family": "Wang", "given": "Han", "initials": "H", "orcid": "0000-0002-6445-2095", "researcher": {"href": "https://publications.scilifelab.se/researcher/9a6c888a8fa948a496030381158af8fb.json"}}, {"family": "Schaeffer", "given": "C\u00e9line", "initials": "C", "orcid": "0000-0001-5883-3951", "researcher": {"href": "https://publications.scilifelab.se/researcher/42307cb5448843d6ab2c6f449da28c57.json"}}, {"family": "Stsiapanava", "given": "Alena", "initials": "A", "orcid": "0000-0001-6560-011X", "researcher": {"href": "https://publications.scilifelab.se/researcher/7dd9089728fe4d41ab4b6db272e3ed76.json"}}, {"family": "Sandin", "given": "Sara", "initials": "S", "orcid": "0000-0001-6071-2761", "researcher": {"href": "https://publications.scilifelab.se/researcher/a52c9c37f07b465781e64958a0edb780.json"}}, {"family": "Carroni", "given": "Marta", "initials": "M", "orcid": "0000-0002-7697-6427", "researcher": {"href": "https://publications.scilifelab.se/researcher/e7f1bc1767024368abcb11a83184994a.json"}}, {"family": "Okumura", "given": "Hiroki", "initials": "H", "orcid": "0000-0001-6222-9634", "researcher": {"href": "https://publications.scilifelab.se/researcher/081a2481c86944cfbb399be7b5400620.json"}}, {"family": "Rampoldi", "given": "Luca", "initials": "L", "orcid": "0000-0002-0544-7042", "researcher": {"href": "https://publications.scilifelab.se/researcher/e09ff90439784c1f81cf41b93e9fcede.json"}}, {"family": "Sandblad", "given": "Linda", "initials": "L", "orcid": "0000-0003-3492-3287", "researcher": {"href": "https://publications.scilifelab.se/researcher/070825e0190a4e9a932e79663d2bc89f.json"}}, {"family": "Jovine", "given": "Luca", "initials": "L", "orcid": "0000-0002-2679-6946", "researcher": {"href": "https://publications.scilifelab.se/researcher/8507a7657f6b434ebc572453aa172b1e.json"}}], "type": "posted-content", "published": "2025-05-11", "journal": {"title": "biorxiv", "issn": null, "issn-l": null, "volume": null, "issue": null, "pages": null}, "abstract": null, "doi": "10.1101/2025.05.09.653020", "pmid": null, "labels": {"Cryo-EM": "Collaborative"}, "xrefs": [], "notes": [], "created": "2025-11-17T11:19:47.083Z", "modified": "2025-12-18T19:16:01.842Z"}, {"entity": "publication", "iuid": "60a28d8ccf1c42869def63646361a306", "links": {"self": {"href": "https://publications.scilifelab.se/publication/60a28d8ccf1c42869def63646361a306.json"}, "display": {"href": "https://publications.scilifelab.se/publication/60a28d8ccf1c42869def63646361a306"}}, "title": "Exploring TRF2-Dependent DNA Distortion Through Single-DNA Manipulation Studies.", "authors": [{"family": "Zhao", "given": "Xiaodan", "initials": "X"}, {"family": "Vogirala", "given": "Vinod Kumar", "initials": "VK"}, {"family": "Liu", "given": "Meihan", "initials": "M"}, {"family": "Zhou", "given": "Yu", "initials": "Y"}, {"family": "Rhodes", "given": "Daniela", "initials": "D", "orcid": "0000-0002-2363-2591", "researcher": {"href": "https://publications.scilifelab.se/researcher/fe153c046b604af492259157dd93e543.json"}}, {"family": "Sandin", "given": "Sara", "initials": "S", "orcid": "0000-0001-6071-2761", "researcher": {"href": "https://publications.scilifelab.se/researcher/a52c9c37f07b465781e64958a0edb780.json"}}, {"family": "Yan", "given": "Jie", "initials": "J", "orcid": "0000-0002-8555-7291", "researcher": {"href": "https://publications.scilifelab.se/researcher/f49e888eb6744ea3b607822def3c86ad.json"}}], "type": "journal article", "published": "2024-02-03", "journal": {"title": "Commun Biol", "issn": "2399-3642", "volume": "7", "issue": "1", "pages": "148", "issn-l": "2399-3642"}, "abstract": "TRF2 is a component of shelterin, a telomere-specific protein complex that protects the ends of mammalian chromosomes from DNA damage signaling and improper repair. TRF2 functions as a homodimer and its interaction with telomeric DNA has been studied, but its full-length DNA-binding properties are unknown. This study examines TRF2's interaction with single-DNA strands and focuses on the conformation of the TRF2-DNA complex and TRF2's preference for DNA chirality. The results show that TRF2-DNA can switch between extended and compact conformations, indicating multiple DNA-binding modes, and TRF2's binding does not have a strong preference for DNA supercoiling chirality when DNA is under low tension. Instead, TRF2 induces DNA bending under tension. Furthermore, both the N-terminal domain of TRF2 and the Myb domain enhance its affinity for the telomere sequence, highlighting the crucial role of multivalent DNA binding in enhancing its affinity and specificity for telomere sequence. These discoveries offer unique insights into TRF2's interaction with telomeric DNA.", "doi": "10.1038/s42003-024-05838-x", "pmid": "38310140", "labels": {"Cryo-EM": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC10838314"}, {"db": "pii", "key": "10.1038/s42003-024-05838-x"}], "notes": [], "created": "2024-11-27T15:05:11.404Z", "modified": "2024-11-27T15:05:11.577Z"}, {"entity": "publication", "iuid": "f77e03baf25e48a9b38fbe8bf3f052e0", "links": {"self": {"href": "https://publications.scilifelab.se/publication/f77e03baf25e48a9b38fbe8bf3f052e0.json"}, "display": {"href": "https://publications.scilifelab.se/publication/f77e03baf25e48a9b38fbe8bf3f052e0"}}, "title": "Ultrastructure and nuclear architecture of telomeric chromatin revealed by correlative light and electron microscopy.", "authors": [{"family": "H\u00fcbner", "given": "Barbara", "initials": "B", "orcid": "0000-0001-5148-0823", "researcher": {"href": "https://publications.scilifelab.se/researcher/742d15c77e554de5a133698a3d0e5605.json"}}, {"family": "von Otter", "given": "Eric", "initials": "E", "orcid": "0000-0002-8769-920X", "researcher": {"href": "https://publications.scilifelab.se/researcher/3285dcd938a6463792660569e619d39e.json"}}, {"family": "Ahsan", "given": "Bilal", "initials": "B", "orcid": "0000-0003-1839-5023", "researcher": {"href": "https://publications.scilifelab.se/researcher/2560fd592afd438797d1dee864884f23.json"}}, {"family": "Wee", "given": "Mei Ling", "initials": "ML"}, {"family": "Henriksson", "given": "Sara", "initials": "S", "orcid": "0000-0003-1615-0583", "researcher": {"href": "https://publications.scilifelab.se/researcher/934e6f18e3c94684be715e7bdc28d9b7.json"}}, {"family": "Ludwig", "given": "Alexander", "initials": "A", "orcid": "0000-0002-0696-5298", "researcher": {"href": "https://publications.scilifelab.se/researcher/74bd2c3af5e24e4695c196bf060b127d.json"}}, {"family": "Sandin", "given": "Sara", "initials": "S", "orcid": "0000-0001-6071-2761", "researcher": {"href": "https://publications.scilifelab.se/researcher/a52c9c37f07b465781e64958a0edb780.json"}}], "type": "journal article", "published": "2022-05-20", "journal": {"title": "Nucleic Acids Res.", "issn": "1362-4962", "volume": "50", "issue": "9", "pages": "5047-5063", "issn-l": "0305-1048"}, "abstract": "Telomeres, the ends of linear chromosomes, are composed of repetitive DNA sequences, histones and a protein complex called shelterin. How DNA is packaged at telomeres is an outstanding question in the field with significant implications for human health and disease. Here, we studied the architecture of telomeres and their spatial association with other chromatin domains in different cell types using correlative light and electron microscopy. To this end, the shelterin protein TRF1 or TRF2 was fused in tandem to eGFP and the peroxidase APEX2, which provided a selective and electron-dense label to interrogate telomere organization by transmission electron microscopy, electron tomography and scanning electron microscopy. Together, our work reveals, for the first time, ultrastructural insight into telomere architecture. We show that telomeres are composed of a dense and highly compacted mesh of chromatin fibres. In addition, we identify marked differences in telomere size, shape and chromatin compaction between cancer and non-cancer cells and show that telomeres are in direct contact with other heterochromatin regions. Our work resolves the internal architecture of telomeres with unprecedented resolution and advances our understanding of how telomeres are organized in situ.", "doi": "10.1093/nar/gkac309", "pmid": "35489064", "labels": {"Integrated Microscopy Technologies Ume\u00e5": "Technology development"}, "xrefs": [{"db": "pii", "key": "6576360"}, {"db": "pmc", "key": "PMC9122609"}], "notes": [], "created": "2022-09-14T08:08:05.702Z", "modified": "2022-11-18T07:44:47.121Z"}]}