{"entity": "researcher", "timestamp": "2026-08-07T18:48:40.504Z", "family": "Da Costa", "given": "Mark", "initials": "M", "orcid": "0000-0001-7713-2608", "affiliations": ["C\u00daRAM SFI Research Centre for Medical Devices, National University of Ireland Galway, Galway, Ireland. abhay.pandit@nuigalway.ie mark.dacosta@nuigalway.ie."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/a18320fbed7b43299aedc829736f4eb9.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/a18320fbed7b43299aedc829736f4eb9"}}, "publications": [{"entity": "publication", "iuid": "bad5b686c16e48c0a5d5984892016a93", "links": {"self": {"href": "https://publications.scilifelab.se/publication/bad5b686c16e48c0a5d5984892016a93.json"}, "display": {"href": "https://publications.scilifelab.se/publication/bad5b686c16e48c0a5d5984892016a93"}}, "title": "Reproducing extracellular matrix adverse remodelling of non-ST myocardial infarction in a large animal model.", "authors": [{"family": "Contessotto", "given": "Paolo", "initials": "P", "orcid": "0000-0002-6990-9883", "researcher": {"href": "https://publications.scilifelab.se/researcher/cd9eff0e573f4f7097fb575bf6479f8e.json"}}, {"family": "Spelat", "given": "Renza", "initials": "R", "orcid": "0000-0001-5794-8976", "researcher": {"href": "https://publications.scilifelab.se/researcher/c7974e486fb24bbf979eb920f9d49380.json"}}, {"family": "Ferro", "given": "Federico", "initials": "F", "orcid": "0000-0002-6023-8227", "researcher": {"href": "https://publications.scilifelab.se/researcher/27810903638948feb4391fa537a7eda3.json"}}, {"family": "Vysockas", "given": "Vaidas", "initials": "V", "orcid": "0000-0002-2179-2348", "researcher": {"href": "https://publications.scilifelab.se/researcher/0c01194476544942a0fd0c153a44487e.json"}}, {"family": "Krivickien\u0117", "given": "Au\u0161ra", "initials": "A", "orcid": "0000-0003-4978-2915", "researcher": {"href": "https://publications.scilifelab.se/researcher/29901a96314b4fc489e3aa1712004ae7.json"}}, {"family": "Jin", "given": "Chunsheng", "initials": "C", "orcid": "0000-0002-0229-102X", "researcher": {"href": "https://publications.scilifelab.se/researcher/458804f3f7274b8a9efe7a90469d0e56.json"}}, {"family": "Chantepie", "given": "Sandrine", "initials": "S"}, {"family": "Chinello", "given": "Clizia", "initials": "C"}, {"family": "Pauza", "given": "Audrys G", "initials": "AG", "orcid": "0000-0001-9635-8872", "researcher": {"href": "https://publications.scilifelab.se/researcher/cb08209745534c21878f607cc414aab8.json"}}, {"family": "Valente", "given": "Camilla", "initials": "C", "orcid": "0000-0003-3084-1102", "researcher": {"href": "https://publications.scilifelab.se/researcher/7cf678853015478daf234ab0f854584e.json"}}, {"family": "Rackauskas", "given": "Mindaugas", "initials": "M", "orcid": "0000-0002-2503-3437", "researcher": {"href": "https://publications.scilifelab.se/researcher/d2edbecde7f94bcfbea7f0776b69bbb2.json"}}, {"family": "Casara", "given": "Alvise", "initials": "A", "orcid": "0000-0002-5056-8732", "researcher": {"href": "https://publications.scilifelab.se/researcher/b3bfb0f2394e44cda5c96d23c6c3b32e.json"}}, {"family": "Zigmantait\u0117", "given": "Vilma", "initials": "V", "orcid": "0000-0002-0410-1533", "researcher": {"href": "https://publications.scilifelab.se/researcher/baa4c86fec6e4f0bbdd6e980a9796ff6.json"}}, {"family": "Magni", "given": "Fulvio", "initials": "F", "orcid": "0000-0002-8663-0374", "researcher": {"href": "https://publications.scilifelab.se/researcher/3d2bb884997b4437924cc2659b823637.json"}}, {"family": "Papy-Garcia", "given": "Dulce", "initials": "D", "orcid": "0000-0003-2225-9331", "researcher": {"href": "https://publications.scilifelab.se/researcher/697039fd70604a79804beb2e8179d9cf.json"}}, {"family": "Karlsson", "given": "Niclas G", "initials": "NG", "orcid": "0000-0002-3045-2628", "researcher": {"href": "https://publications.scilifelab.se/researcher/62e0b7440ff1447c9611d938d185fbc7.json"}}, {"family": "Ereminien\u0117", "given": "Egl\u0117", "initials": "E", "orcid": "0000-0002-8332-788X", "researcher": {"href": "https://publications.scilifelab.se/researcher/8ab7bba2798e40f3b67a791e6be512bc.json"}}, {"family": "Pandit", "given": "Abhay", "initials": "A"}, {"family": "Da Costa", "given": "Mark", "initials": "M", "orcid": "0000-0001-7713-2608", "researcher": {"href": "https://publications.scilifelab.se/researcher/a18320fbed7b43299aedc829736f4eb9.json"}}], "type": "journal article", "published": "2023-02-22", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "14", "issue": "1", "pages": "995", "issn-l": "2041-1723"}, "abstract": "The rising incidence of non-ST-segment elevation myocardial infarction (NSTEMI) and associated long-term high mortality constitutes an urgent clinical issue. Unfortunately, the study of possible interventions to treat this pathology lacks a reproducible pre-clinical model. Indeed, currently adopted small and large animal models of MI mimic only full-thickness, ST-segment-elevation (STEMI) infarcts, and hence cater only for an investigation into therapeutics and interventions directed at this subset of MI. Thus, we develop an ovine model of NSTEMI by ligating the myocardial muscle at precise intervals parallel to the left anterior descending coronary artery. Upon histological and functional investigation to validate the proposed model and comparison with STEMI full ligation model, RNA-seq and proteomics show the distinctive features of post-NSTEMI tissue remodelling. Transcriptome and proteome-derived pathway analyses at acute (7 days) and late (28 days) post-NSTEMI pinpoint specific alterations in cardiac post-ischaemic extracellular matrix. Together with the rise of well-known markers of inflammation and fibrosis, NSTEMI ischaemic regions show distinctive patterns of complex galactosylated and sialylated N-glycans in cellular membranes and extracellular matrix. Identifying such changes in molecular moieties accessible to infusible and intra-myocardial injectable drugs sheds light on developing targeted pharmacological solutions to contrast adverse fibrotic remodelling.", "doi": "10.1038/s41467-023-36350-1", "pmid": "36813782", "labels": {"Glycoproteomics and MS Proteomics": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC9945840"}, {"db": "pii", "key": "10.1038/s41467-023-36350-1"}], "notes": [], "created": "2023-12-04T10:23:03.795Z", "modified": "2024-01-16T13:46:27.924Z"}, {"entity": "publication", "iuid": "903f912e2763491b8c61bc2d0cdb703e", "links": {"self": {"href": "https://publications.scilifelab.se/publication/903f912e2763491b8c61bc2d0cdb703e.json"}, "display": {"href": "https://publications.scilifelab.se/publication/903f912e2763491b8c61bc2d0cdb703e"}}, "title": "Elastin-like recombinamers-based hydrogel modulates post-ischemic remodeling in a non-transmural myocardial infarction in sheep.", "authors": [{"family": "Contessotto", "given": "Paolo", "initials": "P", "orcid": "0000-0002-6990-9883", "researcher": {"href": "https://publications.scilifelab.se/researcher/cd9eff0e573f4f7097fb575bf6479f8e.json"}}, {"family": "Orbani\u0107", "given": "Doriana", "initials": "D", "orcid": "0000-0002-9723-7279", "researcher": {"href": "https://publications.scilifelab.se/researcher/07e26f981b9d44c88b3c72e5ca9acc68.json"}}, {"family": "Da Costa", "given": "Mark", "initials": "M", "orcid": "0000-0001-7713-2608", "researcher": {"href": "https://publications.scilifelab.se/researcher/a18320fbed7b43299aedc829736f4eb9.json"}}, {"family": "Jin", "given": "Chunsheng", "initials": "C", "orcid": "0000-0002-0229-102X", "researcher": {"href": "https://publications.scilifelab.se/researcher/458804f3f7274b8a9efe7a90469d0e56.json"}}, {"family": "Owens", "given": "Peter", "initials": "P", "orcid": "0000-0003-1117-1035", "researcher": {"href": "https://publications.scilifelab.se/researcher/d2c23314e1964d9182b0e292ac0cdb7a.json"}}, {"family": "Chantepie", "given": "Sandrine", "initials": "S", "orcid": "0000-0003-1652-432X", "researcher": {"href": "https://publications.scilifelab.se/researcher/b080f580cda242108279d1eef8580735.json"}}, {"family": "Chinello", "given": "Clizia", "initials": "C", "orcid": "0000-0002-7921-7768", "researcher": {"href": "https://publications.scilifelab.se/researcher/da3a7a0cdecf441ba963745111e322c5.json"}}, {"family": "Newell", "given": "John", "initials": "J", "orcid": "0000-0001-7685-6655", "researcher": {"href": "https://publications.scilifelab.se/researcher/40a38064ac4c40a0b8a221cd906d3861.json"}}, {"family": "Magni", "given": "Fulvio", "initials": "F", "orcid": "0000-0002-8663-0374", "researcher": {"href": "https://publications.scilifelab.se/researcher/3d2bb884997b4437924cc2659b823637.json"}}, {"family": "Papy-Garcia", "given": "Dulce", "initials": "D", "orcid": "0000-0003-2225-9331", "researcher": {"href": "https://publications.scilifelab.se/researcher/697039fd70604a79804beb2e8179d9cf.json"}}, {"family": "Karlsson", "given": "Niclas G", "initials": "NG"}, {"family": "Kilcoyne", "given": "Michelle", "initials": "M", "orcid": "0000-0002-8870-1308", "researcher": {"href": "https://publications.scilifelab.se/researcher/d1d1606f98e540fbb09f7251adf921a2.json"}}, {"family": "Dockery", "given": "Peter", "initials": "P", "orcid": "0000-0001-5103-6589", "researcher": {"href": "https://publications.scilifelab.se/researcher/5355752094da43a39ef13af20f42ae4c.json"}}, {"family": "Rodr\u00edguez-Cabello", "given": "Jos\u00e9 C", "initials": "JC", "orcid": "0000-0002-3438-858X", "researcher": {"href": "https://publications.scilifelab.se/researcher/20649ea23f6d4c85ba4b49a49e474d68.json"}}, {"family": "Pandit", "given": "Abhay", "initials": "A", "orcid": "0000-0002-6292-4933", "researcher": {"href": "https://publications.scilifelab.se/researcher/b4611c209a394949bd971c8b12692fac.json"}}], "type": "journal article", "published": "2021-02-17", "journal": {"title": "Sci Transl Med", "issn": "1946-6242", "volume": "13", "issue": "581", "issn-l": "1946-6234"}, "abstract": "Ischemic heart disease is a leading cause of mortality due to irreversible damage to cardiac muscle. Inspired by the post-ischemic microenvironment, we devised an extracellular matrix (ECM)-mimicking hydrogel using catalyst-free click chemistry covalent bonding between two elastin-like recombinamers (ELRs). The resulting customized hydrogel included functional domains for cell adhesion and protease cleavage sites, sensitive to cleavage by matrix metalloproteases overexpressed after myocardial infarction (MI). The scaffold permitted stromal cell invasion and endothelial cell sprouting in vitro. The incidence of non-transmural infarcts has increased clinically over the past decade, and there is currently no treatment preventing further functional deterioration in the infarcted areas. Here, we have developed a clinically relevant ovine model of non-transmural infarcts induced by multiple suture ligations. Intramyocardial injections of the degradable ELRs-hydrogel led to complete functional recovery of ejection fraction 21 days after the intervention. We observed less fibrosis and more angiogenesis in the ELRs-hydrogel-treated ischemic core region compared to the untreated animals, as validated by the expression, proteomic, glycomic, and histological analyses. These findings were accompanied by enhanced preservation of GATA4+ cardiomyocytes in the border zone of the infarct. We propose that our customized ECM favors cardiomyocyte preservation in the border zone by modulating the ischemic core and a marked functional recovery. The functional benefits obtained by the timely injection of the ELRs-hydrogel in a clinically relevant MI model support the potential utility of this treatment for further clinical translation.", "doi": "10.1126/scitranslmed.aaz5380", "pmid": "33597263", "labels": {"Glycoproteomics and MS Proteomics": "Service"}, "xrefs": [{"db": "pii", "key": "13/581/eaaz5380"}], "notes": [], "created": "2021-12-09T21:53:00.845Z", "modified": "2024-01-16T13:46:30.797Z"}]}